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Biofilm wound

Sarah J Morgan, Soyeon I Lippman, Gilbert E Bautista, Joe J Harrison, Christopher L Harding, Larry A Gallagher, Ann-Chee Cheng, Richard Siehnel, Sumedha Ravishankar, Marcia L Usui, John E Olerud, Philip Fleckman, Randall D Wolcott, Colin Manoil, Pradeep K Singh
While much is known about acute infection pathogenesis, the understanding of chronic infections has lagged. Here we sought to identify the genes and functions that mediate fitness of the pathogen Pseudomonas aeruginosa in chronic wound infections, and to better understand the selective environment in wounds. We found that clinical isolates from chronic human wounds were frequently defective in virulence functions and biofilm formation, and that many virulence and biofilm formation genes were not required for bacterial fitness in experimental mouse wounds...
March 20, 2019: PLoS Pathogens
Derek J Overstreet, Vajra S Badha, John M Heffernan, Erin P Childers, Rex C Moore, Brent L Vernon, Alex C McLaren
Local antimicrobial delivery is a promising strategy for improving treatment of deep surgical site infections (SSIs) by eradicating bacteria that remain in the wound or around its margins after surgical debridement. Eradication of biofilm bacteria can require sustained exposure to high antimicrobial concentrations (we estimate 100-1000 μg/mL sustained for 24 h) which are far in excess of what can be provided by systemic administration. We have previously reported the development of temperature-responsive hydrogels based on poly(N-isopropylacrylamide-co-dimethylbutyrolactone acrylate-co-Jeffamine M-1000 acrylamide) (PNDJ) that provide sustained antimicrobial release in vitro and are effective in treating a rabbit model of osteomyelitis when instilled after surgical debridement...
March 19, 2019: Drug Delivery and Translational Research
Moehamad Orliando Roeslan, Thaweephol Dechatiwongse Na Ayudhya, Boon-Ek Yingyongnarongkul, Sittichai Koontongkaew
AIMS: Clinacanthus nutans (C. nutans) has demonstrated anti-inflammatory activity, however, the active compound generating this activity remains unknown. The aim of this study was to identify the bioactive compound in C. nutans responsible for its anti-inflammatory, in-vitro wound healing, and anti-biofilm activities. MAIN METHODS: A pure compound was isolated from the chloroform extract (CE) of C. nutans leaves by chromatographic techniques and bioassay-guided fractionation...
March 13, 2019: Biomedicine & Pharmacotherapy
Eric D Roche, Emma J Woodmansey, Qingping Yang, Daniel J Gibson, Hongen Zhang, Gregory S Schultz
Biofilms are prevalent in non-healing chronic wounds and implicated in delayed healing. Tolerance to antimicrobial treatments and the host's immune system leave clinicians with limited interventions against biofilm populations. It is therefore essential that effective treatments be rigorously tested and demonstrate an impact on biofilm across multiple experimental models to guide clinical investigations and protocols. Cadexomer iodine has previously been shown to be effective against biofilm in various in vitro models, against methicillin-resistant Staphylococcus aureus biofilm in mouse wounds, and clinically in diabetic foot ulcers complicated by biofilm...
March 13, 2019: International Wound Journal
Alisha R Oropallo
Background: Chronic wounds represent a significant financial burden to the healthcare system and a quality-of-life burden to patients. Many chronic wounds have elevated bioburden in the form of biofilm, which has been associated with delayed wound healing. This study examined the use of a native type I collagen matrix with the antimicrobial polyhexamethylene biguanide (PCMP) in the management of bioburden and treatment of chronic, nonhealing wounds over 12 weeks. Methods: A prospective case series of PCMP enrolled adults ≥18 years old with a nonhealing wound...
January 2019: Plastic and Reconstructive Surgery. Global Open
James Goodwine, Joel Gil, Amber Doiron, Jose Valdes, Michael Solis, Alex Higa, Stephen Davis, Karin Sauer
The formation of biofilms is a developmental process initiated by planktonic cells transitioning to the surface, which comes full circle when cells disperse from the biofilm and transition to the planktonic mode of growth. Considering that pyruvate has been previously demonstrated to be required for the formation of P. aeruginosa biofilms, we asked whether pyruvate likewise contributes to the maintenance of the biofilm structure, with depletion of pyruvate resulting in dispersion. Here, we demonstrate that the enzymatic depletion of pyruvate coincided with the dispersion of established biofilms by S...
March 6, 2019: Scientific Reports
Emily Haesler, Terry Swanson, Karen Ousey, Keryln Carville
OBJECTIVE: To achieve international consensus relating to clinical indicators for a chronic wound, wound infection and biofilm presence to inform the development of international clinical guidance for assessing and managing wound infection. METHOD: An online Delphi consensus process of international key opinion leaders in infection was undertaken. A literature search underpinned the development of issue statements related to terminology, emerging topics and debate in the field of wound infection...
March 2, 2019: Journal of Wound Care
Lauren J Delaney, Daniel MacDonald, Jay Leung, Keith Fitzgerald, Alex M Sevit, John R Eisenbrey, Neil Patel, Flemming Forsberg, Christopher K Kepler, Taolin Fang, Steven M Kurtz, Noreen J Hickok
Despite aggressive peri-operative antibiotic treatments, up to 10% of patients undergoing instrumented spinal surgery develop an infection. Like most implant-associated infections, spinal infections persist through colonization and biofilm formation on spinal instrumentation, which can include metal screws and rods for fixation and an intervertebral cage commonly comprised of polyether ether ketone (PEEK). We have designed a PEEK antibiotic reservoir that would clip to the metal fixation rod and that would achieve slow antibiotic release over several days, followed by a bolus release of antibiotics triggered by ultrasound (US) rupture of a reservoir membrane...
February 28, 2019: Acta Biomaterialia
Daniel J Yoon, Daniel R Fregoso, Duc Nguyen, Vivien Chen, Natasa Strbo, Jaime J Fuentes, Marjana Tomic-Canic, Robert Crawford, Irena Pastar, R Rivkah Isseroff
The prevalence of infection in chronic wounds is well documented in the literature, but not optimally studied due to the drawbacks of current methodologies. Here, we describe a tractable and simplified ex vivo human skin model of infection that addresses the critical drawbacks of high costs and limited translatability. Wounds were generated from excised abdominal skin from cosmetic procedures and cultured, inoculated with Staphylococcus aureus strain UAMS-1, or under aseptic conditions. After three days, the infected wounds exhibited biofilm formation and significantly impaired re-epithelialization compared to the control...
March 1, 2019: Wound Repair and Regeneration
Noëlle Mistretta, Marina Brossaud, Fabienne Telles, Violette Sanchez, Philippe Talaga, Bachra Rokbi
Wall teichoic acid (WTA) are major constituents of Staphylococcus aureus (S. aureus) cell envelopes with important roles in the bacteria's physiology, resistance to antimicrobial molecules, host interaction, virulence and biofilm formation. They consist of ribitol phosphate repeat units in which the ribitol residue is substituted with D-alanine (D-Ala) and N-acetyl-D-glucosamine (GlcNAc). The complete S. aureus WTA biosynthesis pathways was recently revealed with the identification of the two glycosyltransferases, TarM and TarS, respectively responsible for the α- and β-GlcNAc anomeric substitutions...
March 1, 2019: Scientific Reports
Yutaka Ueda, Kota Mashima, Motoyasu Miyazaki, Shuuji Hara, Tohru Takata, Hidetoshi Kamimura, Satoshi Takagi, Shiro Jimi
Methicillin-resistant Staphylococcus aureus (MRSA) forms biofilms on necrotic tissues and medical devices, and causes persistent infections. Surfactants act on biofilms, but their mode of action is still unknown. If used in the clinic, cytotoxicity in tissues should be minimized. In this study, we investigated the inhibitory effect of four different surfactants on MRSA biofilm formation, and found that a nonionic surfactant, polysorbate 80 (PS80), was the most suitable. The biofilm inhibitory effects resulted from the inhibition of bacterial adhesion to substrates rather than biofilm disruption, and the effective dose was less cytotoxic for 3T3 fibroblasts...
February 28, 2019: Scientific Reports
Raghavendra Ramalingam, Chetna Dhand, Chak Ming Leung, Seow Theng Ong, Sathesh Kumar Annamalai, Mohammed Kamruddin, Navin Kumar Verma, Seeram Ramakrishna, Rajamani Lakshminarayanan, Kantha Deivi Arunachalam
Wound care management presents one of the substantial and tenacious challenges to the healthcare systems worldwide. Microbial colonization and subsequent biofilm formation after injury have garnered much attention, as there is an appreciable correlation between biofilms formation and delayed healing in chronic wounds. Nanotechnology has emerged as a potential platform for the management of treating acute and chronic wounds. This study presents the utility of electrospun nanofiber mats containing a natural extract (Gymnema sylvestre) that averts biofilm formation but supports human dermal fibroblasts (hDFs) attachment...
May 2019: Materials Science & Engineering. C, Materials for Biological Applications
Joseph W Gorvetzian, Ryan P Kunkel, Christopher P Demas
Objective: To determine whether use of absorbable antibiotic-imbued beads in chronic soft tissue wounds presents a viable therapeutic modality. Approach: Retrospective analysis of all cases utilizing calcium sulfate antibiotic beads was conducted. Cases comprised complex wound and breast reconstruction performed by the senior author (C.P.D.) over 4 years at the University of New Mexico Hospital. All-cause need for reoperation and reoperation for infection in the 90 days following bead-assisted surgery were compared to traditional surgical intervention in the 90-day period preceding bead-assisted surgery...
February 1, 2019: Advances in Wound Care
Chandan K Sen
Significance: A 2018 retrospective analysis of Medicare beneficiaries identified that ∼8.2 million people had wounds with or without infections. Medicare cost estimates for acute and chronic wound treatments ranged from $28.1 billion to $96.8 billion. Highest expenses were for surgical wounds followed by diabetic foot ulcers, with a higher trend toward costs associated with outpatient wound care compared with inpatient. Increasing costs of health care, an aging population, recognition of difficult-to-treat infection threats such as biofilms, and the continued threat of diabetes and obesity worldwide make chronic wounds a substantial clinical, social, and economic challenge...
February 1, 2019: Advances in Wound Care
Mia Mae Kiamco, Hannah M Zmuda, Abdelrhman Mohamed, Douglas R Call, Yash S Raval, Robin Patel, Haluk Beyenal
Biofilm formation causes prolonged wound infections due to the dense biofilm structure, differential gene regulation to combat stress, and production of extracellular polymeric substances. Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa are three difficult-to-treat biofilm-forming bacteria frequently found in wound infections. This work describes a novel wound dressing in the form of an electrochemical scaffold (e-scaffold) that generates controlled, low concentrations of hypochlorous acid (HOCl) suitable for killing biofilm communities without substantially damaging host tissue...
February 25, 2019: Scientific Reports
Lucia Grassi, Giovanna Batoni, Lisa Ostyn, Petra Rigole, Sara Van den Bossche, Andrea C Rinaldi, Giuseppantonio Maisetta, Semih Esin, Tom Coenye, Aurélie Crabbé
Antimicrobial peptides (AMPs) are promising templates for the development of novel antibiofilm drugs. Despite the large number of studies on screening and optimization of AMPs, only a few of these evaluated the antibiofilm activity in physiologically relevant model systems. Potent in vitro activity of AMPs often does not translate into in vivo effectiveness due to the interference of the host microenvironment with peptide stability/availability. Hence, mimicking the complex environment found in biofilm-associated infections is essential to predict the clinical potential of novel AMP-based antimicrobials...
2019: Frontiers in Microbiology
Steven L Percival, Anne-Marie Salisbury, Rui Chen
Silver is added to an array of commercially available healthcare products including wound dressings. However, overuse of silver is being raised as a potential health concern due to the possible selection of tolerant or resistant bacteria and as a factor that may induce cross resistance to antibiotics. To date, there are only a limited number of studies that have documented evidence of silver resistance in bacteria isolated from medical situations. These studies have indicated low levels of silver resistance in bacteria...
February 22, 2019: Critical Reviews in Microbiology
Rebecca M Haley, Victoria R Qian, Greg D Learn, Horst A von Recum
Surgical sutures are vulnerable to bacterial infections and biofilm formation. At the suture site, pain and undesirable, excess inflammation are additionally detrimental to wound healing. The development of a polymerized cyclodextrin (pCD) coated surgical suture introduces the capability to locally deliver both anti-inflammatory and anti-microbial drugs throughout the phases of acute and chronic healing. Local delivery allows for the improvement of wound healing while reducing related systemic side effects and drug resistance...
February 16, 2019: Journal of Biomedical Materials Research. Part A
Hassan Mahmoudi, Maryam Pourhajibagher, Nasim Chiniforush, Ali Reza Soltanian, Mohammad Yousef Alikhani, Abbas Bahador
OBJECTIVE: To investigate the relationship between biofilm formation and antibiotic resistance patterns in meticillin-resistant and meticillin-sensitive Staphylococcus aureus, isolated from burns. METHODS: In a cross-sectional study, pus/wound swab samples were obtained from burns. Presence of Staphylococcus aureus was confirmed, and biofilm formation-related icaABCDR and eta, etb genes were detected by polymerase chain reaction. Biofilm formation assay was assessed using the microtiter plate method...
February 2, 2019: Journal of Wound Care
Devendra H Dusane, Varun Lochab, Travis Jones, Casey W Peters, Devin Sindeldecker, Amitava Das, Sashwati Roy, Chandan K Sen, Vish V Subramaniam, Daniel J Wozniak, Shaurya Prakash, Paul Stoodley
Electroceutical wound dressings, especially those involving current flow with silver based electrodes, show promise for treating biofilm infections. However, their mechanism of action is poorly understood. We have developed an in vitro agar based model using a bioluminescent strain of Pseudomonas aeruginosa to measure loss of activity and killing when direct current was applied. Silver electrodes were overlaid with agar and lawn biofilms grown for 24 h. A 6 V battery with 1 kΩ ballast resistor was used to treat the biofilms for 1 h or 24 h...
February 14, 2019: Scientific Reports
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