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CD8 T helper

Christoph M Griessinger, Andreas M Schmid, Dominik Sonanini, Barbara F Schörg, Mohamed Ali Jarboui, Daniel Bukala, Natalie Mucha, Birgit Fehrenbacher, Julia Steinhilber, Manuela Martella, Ursula Kohlhofer, Martin Schaller, Lars Zender, Hans-Georg Rammensee, Leticia Quintanilla-Martinez, Martin Röcken, Manfred Kneilling, Bernd J Pichler
Cancer treatment with adoptively transferred tumor-associated antigen-specific CD4+ T-helper cells is a promising immunotherapeutic approach. In the pancreatic cancer model RIP-Tag2, the intraperitoneal (i.p.) application of Tag-specific TH1 cells exhibited a profound antitumoral efficiency. We investigated, whether an intravenous (i.v.) application of Tag-TH1 cells induces an equivalent therapeutic effect. Adoptively transferred fluorescent Tag-TH1 cells revealed a pronounced homing to the tumors after either i...
February 7, 2019: Carcinogenesis
Ruru Guo, Ting Zhang, Xinyu Meng, Zhen Lin, Jinran Lin, Yu Gong, Xuesong Liu, Yuetian Yu, Guilin Zhao, Xianting Ding, Xiaoxiang Chen, Liangjing Lu
Psoriasis (PS) is a systemic, immune-mediated inflammatory disorder. However, the whole lymphocyte compartment and the potential pathologies of PS have not been fully characterized. In the present study, we examined whole lymphocyte subsets and signal transduction proteins using high-dimensional single-cell mass cytometry and a bioinformatics pipeline for an in-depth characterization of the immune cell subsets and protein profiles involved in pathways in the peripheral blood of patients with PS. We identified 15 major immune cell populations in T cell lineages, and characterized various CD3+CD4+T helper and CD3+CD8+T cytotoxic cell populations simultaneously across 24 leukocyte markers and 7 proteins related to the signal transduction pathways...
February 12, 2019: JCI Insight
Julia Bier, Geetha Rao, Kathryn Payne, Henry Brigden, Elise French, Simon J Pelham, Anthony Lau, Helen Lenthall, Emily S J Edwards, Joanne M Smart, Theresa S Cole, Sharon Choo, Avni Y Joshi, Roshini S Abraham, Michael O'Sullivan, Kaan Boztug, Isabelle Meyts, Paul E Gray, Lucinda J Berglund, Peter Hsu, Melanie Wong, Steven M Holland, Luigi D Notarangelo, Gulbu Uzel, Cindy S Ma, Robert Brink, Stuart G Tangye, Elissa K Deenick
BACKGROUND: Gain of function (GOF) mutations in PIK3CD cause a primary immunodeficiency characterized by recurrent respiratory tract infections, susceptibility to herpes virus infections and impaired antibody responses. Previous work revealed defects in CD8+ T and B cells that contribute to this clinical phenotype but less is understood about the role of CD4+ T cells in disease pathogenesis. OBJECTIVE: To dissect the effects of increased phosphoinositide-3 kinase (PI3K) signaling on CD4+ T cell function...
February 6, 2019: Journal of Allergy and Clinical Immunology
D Zhu, R Ding, L J Li, Y M Zheng, H Wang
This study aimed to investigate the effect of T cell subsets with different proportions on renal function and blood lipids in patients with preeclampsia (PE). Ninety pregnant women were included in this experiment and classified into 3 groups: group A (30 with severe PE), group B (30 with mild PE) and group C (30 healthy pregnant women). Clinical characteristics were examined. The ratio of T cell subsets in the three groups was detected by flow cytometry. The relationship between PE and T cell subsets was determined...
February 8, 2019: Journal of Biological Regulators and Homeostatic Agents
Niclas C Blessin, Ronald Simon, Martina Kluth, Kristine Fischer, Claudia Hube-Magg, Wenchao Li, Georgia Makrypidi-Fraune, Björn Wellge, Tim Mandelkow, Nicolaus F Debatin, Doris Höflmayer, Maximilian Lennartz, Guido Sauter, Jakob R Izbicki, Sarah Minner, Franziska Büscheck, Ria Uhlig, David Dum, Till Krech, Andreas M Luebke, Corinna Wittmer, Frank Jacobsen, Eike-Christian Burandt, Stefan Steurer, Waldemar Wilczak, Andrea Hinsch
TIGIT is an inhibitory immune checkpoint receptor and a putative target for novel immune therapies. Here, we analysed two different types of tissue microarrays of healthy lymphatic and various inflamed tissues, colorectal and lung cancers, as well as >1700 tumour samples from 86 different tumour entities for TIGIT and/or PD-1 by bright field and/or multiplex fluorescence immunohistochemistry. TIGIT was detected in CD8+ cytotoxic T cells, CD4+ T helper cells, FOXP3+ regulatory T cells, and NK cells, but not in CD11c+ dendritic cells, CD68+ macrophages, and CD20+ B lymphocytes...
2019: Disease Markers
Nadia Mensali, Amalie Grenov, Niladri Bhusan Pati, Pierre Dillard, Marit Renée Myhre, Gustav Gaudernack, Gunnar Kvalheim, Else Marit Inderberg, Oddmund Bakke, Sébastien Wälchli
Eradication of tumors by the immune system relies on the efficient activation of a T-cell response. For many years, the main focus of cancer immunotherapy has been on cytotoxic CD8 T-cell. However, stimulation of CD4 helper T cells is critical for the promotion and maintenance of immune memory, thus a good vaccine should evoke a two-dimensional T-cell response. The invariant chain (Ii) is required for the MHC class II heterodimer to be correctly guided through the cell, loaded with peptide, and expressed on the surface of antigen presenting cells (APC)...
2019: Oncoimmunology
Axel Lechner, Hans A Schlößer, Martin Thelen, Kerstin Wennhold, Sacha I Rothschild, Ramona Gilles, Alexander Quaas, Oliver G Siefer, Christian U Huebbers, Engin Cukuroglu, Jonathan Göke, Axel Hillmer, Birgit Gathof, Moritz F Meyer, Jens P Klussmann, Alexander Shimabukuro-Vornhagen, Sebastian Theurich, Dirk Beutner, Michael von Bergwelt-Baildon
B lymphocytes are important players in immune responses to cancer. However, their composition and function in head and neck squamous cell carcinoma (HNSCC) has not been well described. Here, we analyzed B cell subsets in HNSCC (n = 38), non-cancerous mucosa (n = 14) and peripheral blood from HNSCC patients (n = 38) and healthy controls (n = 20) by flow cytometry. Intratumoral B cells contained high percentages of activated (CD86+ ), antigen-presenting (CD86+ /CD21- ) and memory B cells (IgD- /CD27+ )...
2019: Oncoimmunology
Pascal Ickrath, Agmal Scherzad, Norbert Kleinsasser, Chr Ginzkey, Rudolf Hagen, Stephan Hackenberg
T cell subpopulations in nasal polyps differ from peripheral lymphocytes in patients with chronic rhinosinusitis with nasal polyps (CRSwNP). However, little is known about the modulatory influence of the inflamed nasal polyp epithelial cells on the phenotype of the T cells. The aim of the present study was to assess this interaction. Tissue and blood samples were collected from 16 patients undergoing paranasal sinus surgery. Polypoid tissue was cultured under air-liquid interface conditions. Subsequently, cluster of differentiation (CD)3/CD28 activated peripheral lymphocytes from the same patients were added...
February 2019: Biomedical Reports
Charlotte M Mousset, Willemijn Hobo, Rob Woestenenk, Frank Preijers, Harry Dolstra, Anniek B van der Waart
The T cell compartment can form a powerful defense against extrinsic (e.g., pathogens) and intrinsic danger (e.g., malignant cells). At the same time, specific subsets of T cells control this process to keep the immune system in check and prevent autoimmunity. A wide variety in T cell functionalities exists, which is dependent on the differentiation and maturation state of the T cells. In this review, we report an overview for the identification of CD4+ T-αβ cells (T-helper (Th)1, Th2, Th9, Th17, Th22, and CD4+ regulatory T cells), CD8+ T-αβ cells (cytotoxic T lymphocyte (Tc)1, Tc2, Tc9, Tc17, and CD8+ regulatory T cells), and their additional effector memory status (naïve, stem cell memory, central memory, effector memory, and effector) using flow cytometry...
February 4, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Cing-Syuan Lei, Jin-Ming Wu, Po-Chu Lee, Ting-Chun Kuo, Po-Da Chen, Yu-Chen Hou, Sung-Ling Yeh, Ming-Tsan Lin
BACKGROUND: Sepsis is a syndrome with CD4+ T-cell dysfunction and dysregulation of T helper (Th) and regulatory T (Treg) cells. Glutamine (Gln) is a nutrient with immunomodulatory properties. This study investigated the effects of dietary Gln pretreatment on Th and Treg cell homeostasis and lung injury in mice with gut-derived polymicrobial sepsis. METHODS: Mice were randomly assigned to 4 groups with 2 control (C and G) and 2 sepsis groups (SC and SG). The C and SC groups were fed a common semipurified diet, whereas the G and SG groups received an identical diet except that part of the casein was replaced by Gln...
February 4, 2019: JPEN. Journal of Parenteral and Enteral Nutrition
Soheil Najafi, Abbas Mirshafiey
T helper 17 (Th17) cells were first described as a novel T helper cell lineage independent from Th1 and Th2 subsets. Th17 cells play vital roles in inflammation and tumor immunity. It causes the dissipation of antitumor immunity and contribution to the survival of tumor cells, worsening tumor growth and metastasis. Tumor-infiltrating Th17 cells were seen innumerous cancers in mice and humans. There has been an association between intratumoral Th17 cell infiltration and both good and bad prognoses. Besides the protumoral roles defined for IL-17 andTh17 cells, several reports have shown that Th17 cells also drive antitumoral immunity...
February 4, 2019: Immunopharmacology and Immunotoxicology
Davood Rostamzadeh, Mehdi Yousefi, Mohammad Reza Haghshenas, Majid Ahmadi, Sanam Dolati, Zohreh Babaloo
The mammalian target of rapamycin (mTOR) is a member of the evolutionary phosphatidylinositol kinase-related kinases (PIKKs). mTOR plays a pivotal role in the regulation of diverse aspects of cellular physiology such as body metabolism, cell growth, protein synthesis, cell size, autophagy, and cell differentiation. Immunologically, mTOR has a fundamental part in controlling and shaping diverse functions of innate and adaptive immune cells, in particular, T-cell subsets differentiation, survival, and metabolic reprogramming to ultimately regulate the fate of diverse immune cell types...
February 2, 2019: Journal of Cellular Physiology
Achilles Ntranos, Vasilis Ntranos, Valentina Bonnefil, Jia Liu, Seunghee Kim-Schulze, Ye He, Yunjiao Zhu, Rachel Brandstadter, Corey T Watson, Andrew J Sharp, Ilana Katz Sand, Patrizia Casaccia
Cell-permeable formulations of metabolites, such as fumaric acid esters, have been used as highly effective immunomodulators in patients with multiple sclerosis and yet their mechanism of action remains elusive. Since fumaric acid esters are metabolites, and cell metabolism is highly intertwined with the epigenetic regulation of gene expression, we investigated whether this metabolic-epigenetic interplay could be leveraged for therapeutic purposes. To this end we recruited 47 treatment-naïve and 35 fumaric acid ester-treated patients with multiple sclerosis, as well as 16 glatiramer acetate-treated patients as a non-metabolite treatment control...
January 28, 2019: Brain: a Journal of Neurology
Sharon Witonsky, Virginia Buechner-Maxwell, Amy Santonastasto, Robert Pleasant, Stephen Werre, Bettina Wagner, Siobhan Ellison, David Lindsay
BACKGROUND: Equine protozoal myeloencephalitis (EPM) is a common and devastating neurologic disease of horses in the United States. Because some EPM-affected horses have decreased immune responses, immunomodulators such as levamisole have been proposed as supplemental treatments. However, little is known about levamisole's effects or its mechanism of action in horses. OBJECTIVE: Levamisole in combination with another mitogen will stimulate a macrophage 1 (M1), dendritic cell 1 (DC1), T-helper 1 (CD4 Th1), and T-cytotoxic (CD8) immune response in equine peripheral blood mononuclear cells (PBMCs) in vitro as compared to mitogen alone...
January 29, 2019: Journal of Veterinary Internal Medicine
Anna Niezgoda, Andrzej Winnicki, Tomasz Kosmalski, Bogna Kowaliszyn, Jerzy Krysiński, Rafał Czajkowski
BACKGROUND: Vitiligo is a chronic skin disorder presenting with depigmentation, the pathogenesis of which is complex but the autoimmune theory is now preferred. Multiple immunologic processes, including stimulation of the T-helper (Th)1 response, formation of autoreactive melanocyte-specific CD8+ T lymphocytes, a decrease in the blood concentration of T regulatory (Treg) cells, and an increase in interleukin (IL)-17 and interferon (IFN) concentration, have been shown to contribute to vitiligo progression and maintenance...
January 25, 2019: Trials
Amir Hossein Mohseni, Sedigheh Taghinezhad-S, Hossein Keyvani, Vadood Razavilar
BACKGROUND: We aimed at constructing Lactococcus lactis strains expressing HPV-16 recombinant E7 (rE7) oncoprotein and examining its overproduction ability followed by optimizing batch and fed-batch fermentations. Thereafter, in order to assess the immunogenicity of recombinant L. lactis cells, C57BL/6 mice were immunized by oral gavage. RESULTS: The results suggested that recombinant strains harboring optiE7 and E7 genes produced a maximum of 4.84 and 1.91 μg/mL of rE7 in static flask experiments, while the corresponding strains gave a maximum yield of 35...
January 24, 2019: BMC Biotechnology
Annie Elong Ngono, Matthew P Young, Maximilian Bunz, Zhigang Xu, Sararat Hattakam, Edward Vizcarra, Jose Angel Regla-Nava, William W Tang, Montarop Yamabhai, Jinsheng Wen, Sujan Shresta
Several Zika virus (ZIKV) vaccines designed to elicit protective antibody (Ab) responses are currently under rapid development, but the underlying mechanisms that control the magnitude and quality of the Ab response remain unclear. Here, we investigated the CD4+ T cell response to primary intravenous and intravaginal infection with ZIKV. Using the LysMCre+Ifnar1fl/fl (myeloid type I IFN receptor-deficient) C57BL/6 mouse models, we identified six I-Ab-restricted ZIKV epitopes that stimulated CD4+ T cells with a predominantly cytotoxic Th1 phenotype in mice primed with ZIKV...
January 2019: PLoS Pathogens
Jing Guan, Yingying Sun, Rong Fu, Huaquan Wang, Erbao Ruan, Xiaoming Wang, Wen Qu, Guojin Wang, Hong Liu, Yuhong Wu, Jia Song, Limin Xing, Lijuan Li, Hui Liu, Chunyan Liu, Zonghong Shao
To investigate if variations in immune and hematopoietic parameters correlated with immunosuppressive therapy (IST) in severe aplastic anemia (SAA) patients.A total of 115 SAA patients who received IST were included. Their immune and hematopoietic functionality changes had been evaluated at 0, 0.5, 1, 2, and 3-year(s) IST.For SAA patients with complete remission (CR), the CD4/CD8T cell ratio continued to increase after a year of IST. The T helper (Th)1/Th2 ratio continued to decrease after 6 months of IST, as did the activated CD8 T cell percentage...
January 2019: Medicine (Baltimore)
Mansour Arab, Maryam Razzaghy-Azar, Zahra Salehi, Maryam Keshavarz, Ensieh Nasli-Esfahani, Mahdi Shekarabi, Maryam Izad
 Type 1 diabetes (T1D) is an autoimmune disease resulting from the damage of pancreatic B-cells mediated by autoreactive CD4+ and CD8+ T cells. In recent years, follicular T helper (Tfh) cells have been recognized as a subpopulation of CD4+ T cells providing help for B cells differentiation and antibody production. In this study, we examined the frequency of circulating CD4+CXCR5+ and CD4+CXCR5+ICOS+ (representing Tfh) cells as well as serum levels of anti-glutamic acid decarboxylase 65 (GAD65) and islet cell autoantibodies (ICA) in children with type I diabetes...
December 3, 2018: Iranian Journal of Allergy, Asthma, and Immunology
Anna Wiszniewsky, Manuel Ritter, Vanessa Krupp, Sandy Schulz, Kathrin Arndts, Heike Weighardt, Samuel Wanji, Achim Hoerauf, Laura E Layland
Worldwide approximately 68 million people are infected with lymphatic filariasis (Lf), provoked by Wuchereria bancrofti, Brugia malayi and Brugia timori. This disease can lead to massive swelling of the limbs (elephantiasis) and disfigurement of the male genitalia (hydrocele). Filarial induced immune regulation is characterised by dominant type 2 helper T cell and regulatory immune responses. In vitro studies have provided evidence that signalling via Toll-like receptor-mediated pathways is triggered by filarial associated factors...
January 15, 2019: Parasitology Research
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