Lene R Sveen, Nicholas Robinson, Aleksei Krasnov, Rose Ruiz Daniels, Marianne Vaadal, Christian Karlsen, Elisabeth Ytteborg, Diego Robledo, Sara Salisbury, Binyam Dagnachew, Carlo C Lazado, Torstein Tengs
In this study, we present the first spatial transcriptomic atlas of Atlantic salmon skin using the Visium Spatial Gene Expression protocol. We utilized frozen skin tissue from four distinct sites, namely the operculum, pectoral and caudal fins, and scaly skin at the flank of the fish close to the lateral line, obtained from two Atlantic salmon (150 g). High quality frozen tissue sections were obtained by embedding tissue in O.C.T media prior to freezing and sectioning. Further, we generated libraries and spatial transcriptomic maps, achieving a minimum of 80 million reads per sample with mapping efficiencies ranging from 79...
September 19, 2023: G3: Genes—Genomes—Genetics