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Circular RNA in HCC

Zheng-Yang Wang, Zhu Zhu, Hua-Fei Wang, Bo Qin, Jing Liu, Xiao-Han Yao, Wen-Cai Li, Kui-Sheng Chen
Circular RNAs have been found to be aberrantly expressed in tumors and their significance in tumorigenesis has been focused on. The role of circDYNC1H1 in hepatocellular carcinoma (HCC) pathogenesis and its relationship with miR-140-5p were explored. The expression of circDYNC1H1, miR-140-5p, and SULT2B1 in HCC tissues and cells was measured, and Pearson's analysis was used to analyze their expression correlation. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays were performed to determine cell proliferation and migration...
March 12, 2019: Journal of Cellular Physiology
Liangliang Xu, Xinfu Feng, Xiangyong Hao, Peng Wang, Yanfang Zhang, Xiaobo Zheng, Lian Li, Shengsheng Ren, Ming Zhang, Mingqing Xu
BACKGROUND: Circular RNAs (circRNAs) play important roles in tumourigenesis and tumour progression. However, the expression profiles and functions of circRNAs in hepatocellular carcinoma (HCC) are largely unclear. METHODS: The expression profiles of circRNAs in HCC were identified through microarray analysis and were validated through quantitative reverse transcription polymerase chain reaction (qRT-PCR). Survival curves were plotted using the Kaplan-Meier method and compared using the log-rank test...
February 22, 2019: Journal of Experimental & Clinical Cancer Research: CR
Huihui Wang, Xiaoguang Yan, Haijun Zhang, Xiaorong Zhan
BACKGROUND Circular RNAs are important regulators in human cancers, including thyroid carcinoma. The circ_0067934 RNA is reported to participate in hepatocellular carcinoma, esophageal squamous cell carcinoma, and lung cancer. Whether it regulates thyroid carcinoma remains unclear. The purpose of this study was to research potential mechanisms of circ_0067934 in thyroid tumors to provide potential new diagnostic and treatment targets. MATERIAL AND METHODS The expression level of circ_0067934 in thyroid tumors, adjacent tissues, and cell lines was measured by qRT-PCR...
February 19, 2019: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
X Li, M Shen
OBJECTIVE: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide, particularly in China. In recent years, numerous studies have investigated the roles of circular RNAs (circRNAs) in tumour development because circRNAs generally act as microRNA (miRNA) sponges to regulate gene expression. However, whether circRNAs are also involved in HCC progression remains largely unknown. MATERIALS AND METHODS: In the present study, we identified a novel circRNA (hsa_circ_103809) and determined its expression in HCC tissues and cell lines by qRT-PCR assays...
February 2019: European Review for Medical and Pharmacological Sciences
Zuhua Li, Ye Zhou, Guohua Yang, Siying He, Xueping Qiu, Lei Zhang, Qianyun Deng, Fang Zheng
BACKGROUND: Hepatocellular carcinoma (HCC) is the most common malignant tumor worldwide. Circular RNAs (circRNAs), a new class of endogenous non-coding RNAs, are widespread and abundant in mammalian cells. Cumulative evidence showed that circRNAs play significant roles in the process of cancer. However, the expression and function of circRNAs in HCC remain to be investigated. METHODS: The expression of circular RNA SMARCA5 (circSMARCA5) in tissues and plasma samples was detected by quantitative real-time polymerase chain reaction (qRT-PCR)...
February 2, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
Bo Lei, Jian Zhou, Xiuyun Xuan, Zhiqiang Tian, Mengjie Zhang, Weiwu Gao, Yuxin Lin, Bing Ni, Hui Pang, Weiping Fan
Circular RNAs (circRNAs) are a large class of noncoding RNAs that have potential regulatory roles in disease pathogenesis and progression. Recently, circRNAs have been found to be expressed in hepatocellular carcinoma (HCC) tissues and involved in the development and metastasis of HCC. However, the significance of circRNAs in peripheral blood mononuclear cells (PBMCs) of HCC patients remains unclear. In this study, RNA sequencing analysis was performed to identify circRNAs from four HCC patients and three healthy controls to determine the expression pattern of circRNAs in the PBMCs and the circRNAs' molecular regulatory networks in HCC pathogenesis...
February 4, 2019: Cancer Medicine
Peisi Kou, Chenyue Zhang, Jiamao Lin, Haiyong Wang
Circular RNA (circRNA), a type of endogenous non-coding RNA, is a closed continuous loop of RNA with no poly(A) tail. Previously, studies have identified that circRNAs are closely associated with several cancer types. However, their function in hepatocellular carcinoma (HCC) has rarely been studied. Therefore, the aim of the current study was to screen differential circRNA expression between HCC tissues and adjacent non-cancerous tissues, and test the potential clinical value of individual circRNAs. CircRNA microarray was used to investigate global circRNA expression profiles...
February 2019: Oncology Letters
Yuanming Ma, Chunjun Zhang, Bo Zhang, Haibo Yu, Qingsong Yu
Hepatocellular carcinoma (HCC) is a heterogeneous malignancy type with limited approaches for treatment. Additionally, inappropriate immune therapy indicates that the understanding the underlying mechanism of HCC is necessary. The aim of the present study was to investigate the influence of a novel circular RNA (circRNA), circRNA of AR-suppressed PABPC1 91 bp (CircARSP91), on immune surveillance induced by natural killer (NK) cells. An in vitro cell cytotoxicity assay was performed to determine the cytotoxicity of NK cells against HCC cells...
January 2019: Oncology Letters
Huifei Zheng, Tengfei Chen, Chang Li, Chun Xu, Cheng Ding, Jun Chen, Sheng Ju, Zhiwei Zhang, Zhipan Liang, Zihan Cui, Jun Zhao
Purpose: Most recently, circular RNAs (circRNAs) were considered playing regulatory roles in tumor initiation and development. The specific function of circRNAs in hepatocellular carcinoma (HCC) remains unknown. This study was designed to detect specific roles of a circRNA hsa_circ_0079299 in HCC. Methods: The expression of hsa_circ_0079299 in HCC and tumor cell lines was detected using quantitative PCR (qPCR). Cell proliferation, migration, cell cycle and apoptosis after overexpression of the circRNA were measured using cell counting kit-8 (CCK8) assay, colony formation, 5-ethynyl-2'-deoxyuridine (EdU) assay, wound healing assay, transwell culture system and flow cytometry...
2019: Cancer Management and Research
Juan Zhang, Yuxia Chang, Lijun Xu, Lijie Qin
Hepatocellular carcinoma (HCC) is one of the most frequent malignancies and a main cause of global cancer mortality. In the past decade, circular RNAs (circRNAs) have been proved to play key roles in various cancers. Previously, circ_0008450 was identified upregulated in HCC tissues by high-throughput circRNA sequencing. In this study, quantitative real-time polymerase chain reaction was used to evaluate the expression level of circ_0008450 in human HCC tumor and corresponding nontumor tissue samples, and the association between circ_0008450 expression and clinicopathologic features of patients with HCC was also analyzed...
December 16, 2018: Journal of Cellular Biochemistry
Haiyang Zhang, Ting Deng, Shaohua Ge, Ying Liu, Ming Bai, Kegan Zhu, Qian Fan, Jialu Li, Tao Ning, Fei Tian, Hongli Li, Wu Sun, Guoguang Ying, Yi Ba
Hepatocellular carcinoma (HCC), the major form of liver cancer, has shown increasing incidence and poor prognosis. Adipose tissue is known to function in energy storage and metabolism regulation by the secretion of adipokines. Circular RNAs (circRNAs), a novel type of noncoding RNA, have recently been recognized as key factors in tumor development, but the role of exosome circRNAs derived from adipose tissues has not been defined yet. Here, adipose-secreted circRNAs were found to regulate deubiquitination in HCC, thus facilitating cell growth...
December 13, 2018: Oncogene
Liman Qiu, Yanbing Huang, Zhenli Li, Xiuqing Dong, Geng Chen, Haipo Xu, Yongyi Zeng, Zhixiong Cai, Xiaolong Liu, Jingfeng Liu
Circular RNAs (circRNAs) can participate in various biological processes, including tumorigenesis, through their microRNA response elements (MREs). Alterations in circRNA profiles during hepatocellular carcinoma (HCC) progression and the clinical significance thereof remain unclear. Here, we present extensive analysis of circRNA profiles in tumor and matched peritumor tissues collected from 10 HCC patients, conducted to identify circRNAs related to HCC progression. A total of 42 dysregulated circRNAs (38 down-regulated and 4 up-regulated) were identified in HCC tumor tissues when compared with matched peritumor tissues, revealing the heterogeneity of circRNA profiles in HCC...
December 8, 2018: Molecular Oncology
Chenxing Zhang, Chenyue Zhang, Jiamao Lin, Haiyong Wang
BACKGROUND/AIMS: An increasing number of studies have suggested that circular RNAs (circRNAs) have vital roles in carcinogenesis and tumor progression. However, the function of circRNAs in hepatocellular carcinoma (HCC) remains poorly characterized. METHODS: We investigated the levels of circRNAs in patients with HCC to identify potential diagnostic biomarkers. We examined circRNA expression profiles in liver tumors and paired non-cancerous liver tissues from three HCC patients with cancer thrombus using a circRNA microarray...
2018: Cellular Physiology and Biochemistry
Xiao Zhang, Yanfeng Xu, Zijun Qian, Weisheng Zheng, Qi Wu, Yan Chen, Guoqing Zhu, Ya Liu, Zhixuan Bian, Wen Xu, Yue Zhang, Fenyong Sun, Qiuhui Pan, Jiayi Wang, Lutao Du, Yongchun Yu
Some types of circular RNA (circRNA) are aberrantly expressed in human diseases including hepatocellular carcinoma (HCC). However, its regulation mechanism and diagnostic roles are largely unknown. Here, we identified that circRNA_104075 (circ_104075) was highly expressed in HCC tissues, cell lines and serum. Mechanistically, HNF4a bound to the -1409 to -1401 region of the circ_104075 promoter to stimulate the expression of circ_104075. Moreover, circ_104075 acted as a ceRNA to upregulate YAP expression by absorbing miR-582-3p...
October 25, 2018: Cell Death & Disease
Changlong Song, Dianqiu Li, Hongyu Liu, Hongyan Sun, Zhen Liu, Lirong Zhang, Yu Hu
Emerging evidence have discovered that circular RNAs (circRNAs) may serve as diagnostic or tumor promising biomarkers. This study aimed to investigate how circular RNA ADAMTS14 (circADAMTS14) regulates microRNA-572/ regulator of calcineurin 1(miR-572/ RCAN1) in hepatocellular carcinoma (HCC). The expression profiles of circRNA/microRNA (mRNA) between HCC tissues and paired adjacent tissues were analyzed via microarray analysis. The expressions of circADAMTS14, miR-572, and RCAN1 were measured by real-time polymerase chain reaction (PCR)...
March 2019: Journal of Cellular Physiology
Shuang Cao, Guohua Wang, Jia Wang, Cheng Li, Le Zhang
In this study, we sought to predict the effects of a certain circular RNA (circRNA), hsa_circ_101280 (also known as hsa_circ_0100929 and hsa_circ_SLAIN1), on hepatocellular carcinoma (HCC) cells and to determine the potential mechanism. After screening differentially expressed circRNAs in HCC tissues through GEO data analysis, hsa_circ_101280 was found to be highly expressed, and its high expression was verified in HCC cell lines with qRT-PCR along with the low expression of its downstream miRNA miR-375. Colony formation and flow cytometry assays showed that both hsa_circ_101280 silencing and miR-375 overexpression restrained proliferation and promoted apoptosis in HCC cells...
October 9, 2018: Immunology and Cell Biology
Anastasiya Kostyusheva, Dmitry Kostyushev, Sergey Brezgin, Elena Volchkova, Vladimir Chulanov
. Chronic hepatitis B virus (HBV) infection has long remained a critical global health issue. Covalently closed circular DNA (cccDNA) is a persistent form of the HBV genome that maintains HBV chronicity. Decades of extensive research resulted in the two therapeutic options currently available: nucleot(s)ide analogs and interferon (IFN) therapy. A plethora of reliable markers to monitor HBV patients has been established, including the recently discovered encapsidated pregenomic RNA in serum, which can be used to determine treatment end-points and to predict the susceptibility of patients to IFN...
October 5, 2018: Genes
Shaling Li, Huimin Gu, Yan Huang, Qian Peng, Rongrong Zhou, Panpan Yi, Ruochan Chen, Zebing Huang, Xingwang Hu, Yun Huang, Daolin Tang
Hepatocellular carcinoma (HCC), one of the most common type of cancers, is highly refractory to most systemic therapies. Understanding the genomic dysregulations, in particularly non-coding RNA (ncRNA) dysregulations, in HCC may provide novel strategies to HCC treatment. In our previous study, we demonstrated the key role of miR-200a-mediated HMGB1/RAGE signaling in HCC carcinogenesis. In the present study, we identified circular RNA (circRNA)-miRNA pair that might modulate the migration of HCC cell lines based on previously reported GEO database (GSE78520 and GSE43445) and investigated the function and molecular mechanism...
2018: Cell Cycle
Emilia Hadziyannis, Andreas Laras
Viral biomarkers are important tools for monitoring chronic hepatitis B virus (HBV) hepatitis B early antigen (HBeAg) negative infection, both in its natural course as well as during and after treatment. The biomarkers consist of antibodies against viral epitopes, viral proteins, and molecular surrogate markers of the quantity and transcriptional activity of the stable episomal HBV covalently closed circular DNA (cccDNA) which is located in the nuclei of the infected hepatocytes. HBV deoxyribonucleic acid (DNA) or else viral load measurement in plasma or serum is a marker of HBV replication of major clinical importance...
September 27, 2018: Genes
Bisha Ding, Weiyang Lou, Liang Xu, Weimin Fan
Hepatocellular carcinoma (HCC) has been one of the most highly lethal cancers. The acquisition of drug resistance accounts for the majority of poor effects of chemotherapy in HCC. Non-coding RNAs (ncRNAs) including miRNAs, long ncRNAs (lncRNAs), and circular RNA (circRNA) have been well-documented to participate in cancer occurrence and progression. Recently, multiple studies have highlighted the key roles of ncRNAs in chemoresistance of HCC. In addition, accumulating evidence has demonstrated that they can serve as biomarkers in diagnosis, treatment, and prognosis of HCC...
October 31, 2018: Bioscience Reports
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