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https://read.qxmd.com/read/30784759/effects-of-mir-181a-5p-abnormal-expression-on-zebrafish-danio-rerio-vascular-development-following-triclosan-exposure
#1
Yan Ma, Luxiu Zang, Danting Wang, Jiahui Jiang, Caihong Wang, Xuedong Wang, Fang Fang, Huili Wang
Triclosan (TCS), one of the important bactericides, is widely used in personal care products, and its chronic exposure leads to severe toxic effects on the growth and development of blood vessels in zebrafish (Danio rerio). Herein, we screened out three differentially expressed miRNAs (miR-181a-5p, miR-132-3p and miR-128-3p) by sequencing and qRT-PCR analyses of 4-96-hpf TCS-exposed zebrafish, among which miR-181a-5p was found to regulate many signaling pathways involved in fatty acid biosynthesis and phosphatidylimositol signaling systems...
February 11, 2019: Chemosphere
https://read.qxmd.com/read/30783440/regulation-of-tgf-%C3%AE-1-on-pi3kc3-and-its-role-in-hypertension-induced-vascular-injuries
#2
Qin Zhang, Hu Liu, Jun Yang
The aim of the present study was to investigate the expression and role of transforming growth factor (TGF)-β1/phosphatidylinositol 3-kinase catalytic subunit type 3 (PI3KC3) in the peripheral blood in patients with hypertension. A total of 28 patients with primary hypertension and 20 healthy control subjects were included. Peripheral blood samples were collected. The mRNA and protein expression levels were detected by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively...
March 2019: Experimental and Therapeutic Medicine
https://read.qxmd.com/read/30783422/clinical-efficacy-evaluation-of-treatment-of-different-degrees-of-retinal-vein-occlusion-with-ranibizumab-combined-with-an-argon-ion-laser
#3
Dingding Wang, Xiaoyi Wang, Kunfang Wu, Juanjuan Wang, Guihua Xu, Zilin Chen
The aim of this study was to evaluate the clinical efficacy of an intravitreal injection of ranibizumab combined with argon ion laser photocoagulation therapy in the treatment of different degrees of central retinal vein occlusion (CRVO). A total of 112 CRVO patients including 25 cases of trunk occlusion, 50 cases of branch occlusion and 37 cases of hemiretinal vein-occlusion were enrolled in this study. Patients were treated with an intravitreal injection of 0.5 mg ranibizumab, followed by argon ion laser photocoagulation therapy after 7 days...
March 2019: Experimental and Therapeutic Medicine
https://read.qxmd.com/read/30772801/isorhamnetin-protects-endothelial-cells-model-crl1730-from-oxidative-injury-by-hydrogen-peroxide
#4
Cheng Jiayi, Ning Tianyi, Teng Dan, Kang Tingguo, Wang Qingfeng, Zhang Qianqian
To explore the protective effect and mechanism of isorhamnetin against oxidative injury caused by H2 O2 to endothelial cell strain CRL1730 of human umbilical vein. H2 O2 and endothelial cell strain CRL1730 were used, as a model of injured endothelial cell. Three levels of crude drugs areorhamnetin, 22.8μg/ml, 11.4μg/mL and 5.7μg/mL was added to the injured cell strain CRL1730 respectively. The cell injury was measured in terms of necrotic rate, quantities of von Wilebr and factor (vWf) and thrombomodulin (TM), lactate dehydrogenase (LDH) and intracellular free calcium ions through flow cytometry, ELISA, fluorescent spectrometer and laser scanning confocal microscopy respectively...
January 2019: Pakistan Journal of Pharmaceutical Sciences
https://read.qxmd.com/read/30771831/histopathologic-differences-in-the-endovenous-laser-ablation-between-jacketed-and-radial-fibers-in-an-ex-vivo-dominant-extrafascial-tributary-of-the-great-saphenous-vein-in-an-in-vitro-model-using-histology-and-immunohistochemistry
#5
Henry F Ashpitel, Emma B Dabbs, Francisco J Salguero, Jaya L Nemchand, Roberto M La Ragione, Mark S Whiteley
OBJECTIVE: The study aimed to investigate the biologic effects of the 1470-nm endovenous laser (EVL), with a jacketed fiber and a radial fiber, during EVL ablation of an ex vivo dominant extrafascial tributary of the great saphenous vein in our in vitro model by histology and immunohistochemistry. METHODS: Ten segments of the dominant extrafascial tributary of the great saphenous vein were harvested by a consultant vascular surgeon from patients during routine varicose vein surgery...
March 2019: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://read.qxmd.com/read/30769060/endovenous-laser-ablation-with-1470-nm-diode-with-tumescence-anesthesia-and-saphenofemoral-ligation-propensity-score-match-comparison
#6
Marco Leopardi, Alessia Salerno, Angelica Dante, Vincenza Cofini, Stefano Necozione, Marco Ventura
OBJECTIVE: The aim of this study is to compare two groups of patients treated for great saphenous vein (GSV) reflux with open surgical saphenofemoral ligation (SFL) and EndoVenous Laser Ablation (EVLA) MATERIALS AND METHODS: Consecutive patients with primary unilateral GSV reflux undergoing EVLA treatment since 2014 were enrolled, and another series of patients treated with SFL was considered. The patients were stratified according to treatment and the results were compared using the propensity score (1:1)...
February 12, 2019: Annals of Vascular Surgery
https://read.qxmd.com/read/30744440/efficient-exosome-delivery-in-refractory-tissues-assisted-by-ultrasound-targeted-microbubble-destruction
#7
Wenqi Sun, Zhelong Li, Xueying Zhou, Guodong Yang, Lijun Yuan
Recently, exosomes have been emerged as promising drug delivery carriers, while certain tissues are intrinsically resistant to exosomes. Therapeutically improving the drug delivery efficiency in these tissues/organs would certainly broaden the potential application of exosomes in future. Ultrasound-targeted microbubble destruction (UTMD) is a promising technique for non-invasive, targeted drug delivery. In this study, we explore the possibility that UTMD assists exosome delivery in these intrinsically resistant tissues...
December 2019: Drug Delivery
https://read.qxmd.com/read/30742735/laser-ablation-of-embryonic-veins-in-children
#8
Mark Bittles, Diana Jodeh, Jennifer Mayer, Michael Gallant, S Alex Rottgers
INTRODUCTION: Klippel-Trenaunay syndrome (KTS) and related overgrowth syndromes are rare conditions that often present to academic pediatric units and multidisciplinary vascular anomaly clinics. These conditions are commonly associated with abnormalities of the superficial and deep venous structures and carry an increased risk of venous thromboembolism. METHODS: A retrospective chart review was completed of all patients treated at Johns Hopkins All Children's Hospital with endovenous laser therapy (EVLT) for management of dilated embryonic veins in the setting of limb overgrowth...
February 11, 2019: Pediatrics International: Official Journal of the Japan Pediatric Society
https://read.qxmd.com/read/30741534/non-invasive-tracking-and-regenerative-capabilities-of-transplanted-human-umbilical-cord-derived-mesenchymal-stem-cells-labeled-with-i-iii-iv-semiconducting-nanocrystals-in-liver-injured-living-mice
#9
Shashank Shankar Chetty, Selvarasu Praneetha, Kavitha Govarthanan, Rama Shanker Verma, Arumugam Vadivel Murugan
Acute liver injury is a critical syndrome ascribed to prevalent death of hepatocyte and imperatively requires a liver transplantation. Such methodology is certainly hampered due to deficit of healthy donors. In this regard, stem cells-based regenerative therapies are attractive in repairing injured tissues and organs for medical applications. However, it is crucial to understand the migration, engraftment and regeneration capabilities of transplanted stem cells in the living animal models. For the first time, we demonstrate rapid labeling of umbilical cord-derived mesenchymal stem cells (MSCs) with near-infrared (NIR) fluorescent CuInS2-ZnS nanocrystals (CIZS-NCs) to develop an innovative nano-bioconjugates (MSCs-CIZS-NBCs) that exhibit 98% labeling efficiency...
February 11, 2019: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/30722692/early-scatter-laser-photocoagulation-promotes-the-formation-of-collateral-vessels-in-branch-retinal-vein-occlusion
#10
Seoung Hyun An, Woo Jin Jeong
PURPOSE: To investigate the effect of early-scatter laser photocoagulation on the formation of collateral vessels in branch retinal vein occlusion. METHODS: The medical records of 40 cases (40 patients) of branch retinal vein occlusion with macular edema were retrospectively reviewed. Of them, 23 patients were treated with intravitreal bevacizumab injection and 17 patients underwent intravitreal bevacizumab injection with additional laser treatment. Early-scatter laser photocoagulation was applied for capillary non-perfusion areas, regardless of retinal neovascularization...
February 5, 2019: European Journal of Ophthalmology
https://read.qxmd.com/read/30707327/effects-of-1064-nm-nd-yag-long-pulse-laser-on-polidocanol-microfoam-injected-for-varicose-vein-treatment-a-controlled-observational-study-of-404-legs-after-5-year-long-treatment
#11
Javier Moreno-Moraga, Mihail L Pascu, Justo M Alcolea, Adriana Smarandache, Josefina Royo, Fernández David, Mario A Trelles
Sclerotherapy continues to be the treatment of choice for varicose veins in the legs. However, isolated treatment using microfoam or lasers requires a high number of sessions to eliminate them. In 2013, we published results about the efficacy and safety 3 years after the combined treatment with microfoam injections and subsequent application of Nd:YAG laser. The aim of this paper is to clinically evaluate the treatment of varices in a control visit after 5 years, when polidocanol microfoam is used and is immediately irradiated in the tissue with 1064-nm Nd:YAG laser beam...
February 1, 2019: Lasers in Medical Science
https://read.qxmd.com/read/30706162/microglia-contribute-to-the-glia-limitans-around-arteries-capillaries-and-veins-under-physiological-conditions-in-a-model-of-neuroinflammation-and-in-human-brain-tissue
#12
Emely Joost, Marta J C Jordão, Bianca Mages, Marco Prinz, Ingo Bechmann, Martin Krueger
Microglia represent resident immune cells of the central nervous system (CNS), which have been shown to be involved in the pathophysiology of practically every neuropathology. As microglia were described to participate in the formation of the astroglial glia limitans around CNS vessels, they are part of the neurovascular unit (NVU). Since the NVU is a highly specialized structure, being functionally and morphologically adapted to differing demands in the arterial, capillary, and venous segments, the present study was aimed to systematically investigate the microglial contribution to the glia limitans along the vascular tree...
January 31, 2019: Brain Structure & Function
https://read.qxmd.com/read/30705242/endovenous-ablation-and-surgery-in-great-saphenous-vein-reflux-a-systematic-review-and-network-meta-analysis-of-randomised-controlled-trials-protocol
#13
Boonying Siribumrungwong, Kanoklada Srikuea, Saritphat Orrapin, Thoetphum Benyakorn, Kittipan Rerkasem, Ammarin Thakkinstian
INTRODUCTION: Endovenous ablations are the new standard procedures for treatment of great saphenous vein reflux including endovenous laser ablation (EVLA), radio frequency ablation (RFA), endovenous steam ablation (EVSA), mechanochemical ablation (MOCA), cyanoacrylate injection and ultrasound-guided foam sclerotherapy (UGFS). EVLA and RFA have demonstrated similar anatomical success for short-term outcome, but results are controversial for longer term (≥5 years). Additional evidences from randomised controlled trials have been published...
January 30, 2019: BMJ Open
https://read.qxmd.com/read/30703390/a-novel-rat-model-of-cerebral-artery-occlusion-complicated-with-prior-venous-stagnation
#14
Nan-Nan Zhang, Kai-Tao Zhao, Zi-Ai Zhao, Wan-Li Chen, Hai-Bin Xu, Hui-Sheng Chen
BACKGROUND: To establish a novel rat model of middle cerebral artery occlusion (MCAO) complicated with prior venous stagnation, and to investigate the role of cerebral venous drainage in neural injury after acute cerebral infarction. NEW METHOD: Eighteen SD rats were randomly divided into two groups: control group and jugular vein ligation group. The left jugular vein ligation was performed to produce the jugular venous stagnation. In the control group, the jugular vein was exposed but not ligated...
January 28, 2019: Journal of Neuroscience Methods
https://read.qxmd.com/read/30697430/the-nd-yag-laser-as-first-line-treatment-for-fibrin-pupillary-block-glaucoma-following-uncomplicated-cataract-surgery
#15
Selina Khan, Mohamed Elashry
A 43-year-old lady presented with painful, loss of vision in the right eye 2 weeks after cataract surgery. This is on a background of previous central retinal vein occlusion in the right eye and bilateral diabetic retinopathy. Her past medical history included mixed connective tissue disease for which she is on systemic immunosuppressants. Ocular examination revealed significant corneal oedema, a shallow anterior chamber with an intraocular pressure of 45 mmHg. Intraocular pressure lowering medications were administered and repeat examination identified a fine fibrinous membrane attached to the pupillary margin with a substantial third space that had formed between the posterior edge of the membrane and the anterior lens surface...
January 2019: Oxford Medical Case Reports
https://read.qxmd.com/read/30696892/detecting-and-mapping-harmful-chemicals-in-fruit-and-vegetables-using-nanoparticle-enhanced-laser-induced-breakdown-spectroscopy
#16
Xiande Zhao, Chunjiang Zhao, Xiaofan Du, Daming Dong
Residues of harmful chemicals in fruit and vegetables pose risks to human health. Ordinary laser-induced breakdown spectroscopy (LIBS) techniques are unsatisfactory for detecting harmful chemicals in fruit and vegetables. In this study, we applied metal nanoparticles to fruit and vegetables samples to improve the ability of LIBS to detect trace pesticide and heavy metal residues in the samples. The nanoparticle-enhanced LIBS technique gave pesticide residue detection limits for fruit and vegetables two orders of magnitude lower than achieved using standard LIBS and heavy metal detection limits markedly better than achieved using standard LIBS...
January 29, 2019: Scientific Reports
https://read.qxmd.com/read/30695815/understanding-quality-of-life-impact-in-people-with-retinal-vein-occlusion-a-qualitative-inquiry
#17
Mallika Prem Senthil, Jyoti Khadka, Jagjit S Gilhotra, Sumu Simon, Eva K Fenwick, Ecosse Lamoureux, Konrad Pesudovs
BACKGROUND: Although being the second most common sight-threatening retinal vascular disease after diabetic retinopathy, the patient-centred impact of retinal vein occlusion has not been well studied. This study aims to understand the quality of life issues in people with retinal vein occlusion using a qualitative methodology. METHODS: In-depth semi-structured interviews were conducted with 17 patients with retinal vein occlusion. All the interviews were digitally recorded and transcribed verbatim...
January 29, 2019: Clinical & Experimental Optometry: Journal of the Australian Optometrical Association
https://read.qxmd.com/read/30679981/comparative-study-in-leg-telangiectasias-treatment-with-nd-yag-laser-and-sclerotherapy
#18
Gabriel Ianosi, Simona Ianosi, Madalina Xenia Calbureanu-Popescu, Cristina Tutunaru, Daniela Calina, Daniela Neagoe
Telangiectasias and reticular veins of the lower extremities are common lesions. Sclerotherapy is considered the gold standard for treatment. The aim of our prospective randomized study was to compare the efficacy and safety of hypertonic 20% saline/2% lignocaine (HS) versus polidocanol 0.5% (POL) versus long-pulsed neodymium:ytrium aluminium garnet (Nd:YAG) laser (LAS) treatments of leg telangiectasias in women, using each patient as her own control. We included in this study 285 women with primary leg telangiectasias and reticular veins (C1A Ep AS1 PN ) in order to be treated with sclerotherapy or laser...
February 2019: Experimental and Therapeutic Medicine
https://read.qxmd.com/read/30663158/exosomes-from-human-umbilical-cord-mesenchymal-stem-cells-enhance-fracture-healing-through-hif-1%C3%AE-mediated-promotion-of-angiogenesis-in-a-rat-model-of-stabilized-fracture
#19
Yuntong Zhang, Zichen Hao, Panfeng Wang, Yan Xia, Jianghong Wu, Demeng Xia, Shuo Fang, Shuogui Xu
OBJECTIVES: Exosomes, as important players in intercellular communication due to their ability to transfer certain molecules to target cells, are believed to take similar effects in promoting bone regeneration with their derived stem cells. Studies have suggested that umbilical cord mesenchymal stem cells (uMSCs) could promote angiogenesis. This study investigated whether exosomes derived from uMSCs (uMSC-Exos) could enhance fracture healing as primary factors by promoting angiogenesis...
January 20, 2019: Cell Proliferation
https://read.qxmd.com/read/30658846/development-of-a-novel-model-of-central-retinal-vascular-occlusion-and-the-therapeutic-potential-of-the-adrenomedullin-receptor-activity-modifying-protein-2-system
#20
Kazutaka Hirabayashi, Masaaki Tanaka, Akira Imai, Yuichi Toriyama, Yasuhiro Iesato, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Hisaka Kawate, Megumu Tanaka, Kun Dai, Nanqi Cui, Yangxuan Wei, Keisei Nakamura, Shiho Iida, Shuhei Matsui, Akihiro Yamauchi, Toshinori Murata, Takayuki Shindo
Central retinal vein occlusion (CRVO) is an intractable disease that causes visual acuity loss with retinal ischemia, hemorrhage, and edema. In this study, we developed an experimental CRVO model in mice and evaluated the therapeutic potential of the pleiotropic peptide adrenomedullin (ADM) and its receptor activity-modifying protein 2 (RAMP2). The CRVO model, which had phenotypes resembling those seen in the clinic, was produced by combining i.p. injection of Rose bengal, a photoactivator dye enhancing thrombus formation, with laser photocoagulation...
January 10, 2019: American Journal of Pathology
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