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Yingchao Ma, Linwen He, Li Huan, Xiaoping Lu, Guangce Wang
A mutant strain of Pyropia yezoensis, strain E, was isolated from the free living conchocelis of a pure strain (NA) treated with ethyl methane sulfonate. The incremental quantities of young strain E blades were higher than those of NA after 14 d of cultivation, indicating that young blades of mutant strain E released more archeospores. The mean length and weight of large E blades were both over three times greater than those of NA after 4 weeks of cultivation. The photosynthetic parameters (Fv/Fm, Y[I], Y[II], and O2 evolution rate) and pigment contents (including phycoerythrin and phycocyanin) of strain E blades were higher than those of NA (p<0...
February 5, 2019: Journal of Phycology
Shan Gao, Zhen Chi, Hailong Chen, Zhenbing Zheng, Yuxiang Weng, Guangce Wang
The thylakoid membranes of plants play a critical role in electron transfer and energy fixation, and are highly dynamic. So far, studies on the thylakoid membranes have mainly focused on microalgae and higher plants, yet very little information is available on the macroalgal thylakoids. Here, we studied the structure and organization of the thylakoid membranes in Ulva prolifera, a representative species of the green macroalgae. We found U. prolifera had few but long loosely-stacked membranes which lack the conventional grana found in higher plants...
October 8, 2018: Plant & Cell Physiology
Bin Yu, Jianfeng Niu, Jianhua Feng, Meiling Xu, Xiujun Xie, Wenhui Gu, Shan Gao, Guangce Wang
Pyropia yezoensis can survive the severe water loss that occurs during low tide, making it an ideal species to investigate the acclimation mechanism of intertidal seaweed to special extreme environments. In this study, we determined the effects of high salinity on photosynthesis using increasing salinity around algal tissues. Both electron transport rates, ETR (I) and ETR (II), showed continuous decreases as the salinity increased. However, the difference between these factors remained relatively stable, similar to the control...
2018: Frontiers in Plant Science
Xue Sun, Jun Wu, Guangce Wang, Yani Kang, Hong Sain Ooi, Tingting Shen, Fangjun Wang, Rui Yang, Nianjun Xu, Xiaodong Zhao
BACKGROUND: Red algae are economically valuable for food and in industry. However, their genomic information is limited, and the genomic data of only a few species of red algae have been sequenced and deposited recently. In this study, we annotated a draft genome of the macroalga Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta). RESULTS: The entire 88.98 Mb genome of Gp. lemaneiformis 981 was generated from 13,825 scaffolds (≥500 bp) with an N50 length of 30,590 bp, accounting for approximately 91% of this algal genome...
May 25, 2018: BMC Plant Biology
Peipei Zhao, Wenhui Gu, Aiyou Huang, Songcui Wu, Changheng Liu, Li Huan, Shan Gao, Xiujun Xie, Guangce Wang
Iron is a limiting factor that controls the phytoplankton biomass in the modern ocean, and iron fertilization of the ocean could lead to blooms dominated by diatoms. Thus, iron plays an important role in controlling the distribution of diatoms. In this study, we measured the growth rate and photosynthetic activity of the model diatom Phaeodactylum tricornutum cultured under different iron concentrations and found that it grew more rapidly and had a much higher photosynthetic efficiency under higher iron concentrations...
February 2018: Journal of Phycology
Li Huan, Wenhui Gu, Shan Gao, Guangce Wang
Free-floating Ulva prolifera is one of the causative species of green tides. When green tides occur, massive mats of floating U. prolifera thalli accumulate rapidly in surface waters with daily growth rates as high as 56%. The upper thalli of the mats experience environmental changes such as the change in carbon source, high salinity, and desiccation. In this study, the photosynthetic performances of PSI and PSII in U. prolifera thalli exposed to different atmospheric carbon dioxide (CO2 ) levels were measured...
December 2016: Journal of Phycology
Jian Sun, Liang Guo, Qianqian Li, Yangguo Zhao, Mengchun Gao, Zonglian She, Guangce Wang
The effects of heat pretreatment on waste sludge hydrolysis were investigated in this study. Heat pretreatment was conducted at 65°C, 80°C, 100°C and 121°C for 5min, 10min, 15min, 20min, 25min and 30min. Not only analyzed the changes of SCOD (Soluble chemical oxygen demand), carbohydrate and protein, but also evaluated the structural and functional properties of organics in extracellular polymeric substances (EPS) and dissolved organic matters (DOM) by using three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy with fluorescence regional integration (FRI) analysis...
November 2016: Bioresource Technology
Hui Wang, Apeng Lin, Wenhui Gu, Li Huan, Shan Gao, Guangce Wang
Sporulation and spore release are essential phases of the life cycle in algae and land plants. Ulva prolifera, which is an ideal organism for studying sporulation and spore release, was used as the experimental material in the present study. The determination of photosynthetic parameters, combined with microscopic observation, treatment with photosynthetic inhibitors, limitation of carbon acquisition, and protein mass spectrometry, was employed in this experiment. Cycle electron transport (CEF) was found enhanced at the onset of sporangia formation...
April 22, 2016: Scientific Reports
Hongjin Qiao, Guangce Wang, Xiaojuan Zhang
Heterotrophic and anaerobic microalgae are of significance in both basic research and industrial application. A microalga strain was isolated from a wastewater treatment pond and identified as Chlorella sorokiniana Shihira et W. R. Krauss GXNN01 in terms of morphology, physiology, and phylogeny. The strain grows rapidly in heterotrophic or mixotrophic conditions with addition of various carbon sources, and even in anaerobic conditions. The maximum growth rate reached 0.28 d(-1) when using d,l-malate as the carbon source, and the protein content of the microalgae was 75...
October 2009: Journal of Phycology
Shan Gao, Guangce Wang, Ruiling Yang, Xiujun Xie, Guanghua Pan, Pu Xu, Jianyi Zhu
The formation of archeospores is characteristic of Porphyra yezoensis Ueda and is important for Porphyra aquaculture. Recently, it has been regarded as a valuable seed source for propagation of thalli in mariculture. Cell wall composition changes are associated with archeospore formation in P. yezoensis. Here, we report changes of cell walls of P. yezoensis during archeospore formation. The surfaces of vegetative cells that were originally smooth became rougher and more protuberant as archeosporangia were formed...
August 2011: Journal of Phycology
Xiaojie Wu, Jianfeng Niu, Aiyou Huang, Meiling Xu, Guangce Wang
Accurate gene quantification depends on the use of an appropriate internal control gene, which should be verified before its use for normalizing data. Housekeeping genes, which are expressed at relatively constant levels, are generally regarded as candidate internal control genes. To determine the ideal internal control for gene expression profiles for Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) at different life-history stages, we used absolute quantification to assess the expression levels of six housekeeping genes (18S ribosomal RNA, 30S ribosomal protein, glyceraldehyde-3-phosphate dehydrogenase, elongation factor 3, alpha-tubulin, and beta-tubulin) at the sporophyte and gametophyte stages...
August 2012: Journal of Phycology
Hongjin Qiao, Guangce Wang, Kai Liu, Wenhui Gu
The culture of microalgae using organic carbon sources decreases the cost of operation in closed systems. The effect of carbon sources on microalgae is thus an interesting problem in not only theoretical research but also practical production. The short-term effects of acetate and microaerobic conditions on the growth, photosynthesis, and respiration of the green microalga Chlorella sorokiniana I. Shihira & R.W. Krauss GXNN 01 were described after acetate addition to autotrophic cultures. As the acetate concentration increased, cells needed a longer lag phase to grow, and 243...
August 2012: Journal of Phycology
Zhaojun Jia, Jianfeng Niu, Li Huan, Xiaojie Wu, Guangce Wang, Zhaojun Hou
Porphyra haitanensis (T. J. Chang & B. F. Zheng) is an important economic alga found off the southern coast of China. It has evolved a strong tolerance against stress, which is an important survival characteristic. Cyclophilin has been shown to be involved in the stress response of plants and algae. To investigate the tolerance against stress in Porphyra, we isolated the cyclophilin PhCYP18 gene (Accession number JQ413239) and measured its expression over different generations and stress conditions. In P. haitanensis, cyclophilin PhCYP18 accumulated more in the filamentous sporophyte generation than in the blade gametophyte generation...
February 2013: Journal of Phycology
Li Huan, Linwen He, Baoyu Zhang, Jianfeng Niu, Apeng Lin, Guangce Wang
Gracilaria lemaneiformis (Bory de Saint-Vincent) Greville, an important marine alga, has great economic and nutritional value. However, during the nonreproductive period, it is difficult to distinguish the sporophyte, male gametophyte, and female gametophyte from each other by appearance. Amplified fragment length polymorphism (AFLP) is a multilocus marker technique, which was used in this study to identify markers associated with G. lemaneiformis sex type. By applying 80 primer combinations in the screening process, three fragments were found that were specific to male or female forms of the alga...
August 2013: Journal of Phycology
Linwen He, Jianyi Zhu, Qinqin Lu, Jianfeng Niu, Baoyu Zhang, Apeng Lin, Guangce Wang
Pyropia yezoensis (Ueda) M. S. Hwang et H. G. Choi (previously called Porphyra yezoensis) is an economically important alga. The blades generated from conchospores are genetic chimeras, which are not suitable for genetic similarity analysis. In this study, two types of blades from a single filament of P. yezoensis sporophyte filament were obtained. One type, ConB, consisted of 40 blades that had germinated from conchospores. The other type, ArcB, consisted of 88 blades that had germinated from archeospores released from ConB...
June 2013: Journal of Phycology
Shan Gao, Zhenbing Zheng, Li Huan, Guangce Wang
Photosynthetic performances and glucose-6-phosphate dehydrogenase (G6PDH) activity in Physcomitrella patens changed greatly during salt stress and recovery. In P. patens, the cyclic electron flow around photosystem (PS) I was much more tolerant to high salt stress than PSII. After high salt stress, the PSII activity recovered much more slowly than that of PSI, which was rapidly restored to pretreatment levels even as PSII was almost inactivate. This result suggested that after salt stress the recovery of the cyclic electron flow around PSI was independent of PSII activity...
February 18, 2016: Scientific Reports
Xiujun Xie, Aiyou Huang, Wenhui Gu, Zhengrong Zang, Guanghua Pan, Shan Gao, Linwen He, Baoyu Zhang, Jianfeng Niu, Apeng Lin, Guangce Wang
The development of microalgae on an industrial scale largely depends on the economic feasibility of mass production. High light induces productive suspensions during cultivation in a tubular photobioreactor. Herein, we report that high light, which inhibited the growth of Chlorella sorokiniana under autotrophic conditions, enhanced the growth of this alga in the presence of acetate. We compared pigments, proteomics and the metabolic flux ratio in C. sorokiniana cultivated under high light (HL) and under low light (LL) in the presence of acetate...
February 2016: New Phytologist
Xiaoping Lu, Li Huan, Shan Gao, Linwen He, Guangce Wang
Pyropia yezoensis (Bangiales, Rhodophyta) is a representative species of high-intertidal macroalgae, whose blades can tolerate extreme stresses, such as salt stress and desiccation. In this study, the photosystem (PS) responses of P. yezoensis blades under salt stress were studied. Our results showed that when the effective photochemical quantum yield of PS (Y) II decreased to almost zero under high salt stress, YI still had a relatively high activity rate. PSII was therefore more sensitive to salt stress than PSI...
April 2016: Physiologia Plantarum
Songcui Wu, Aiyou Huang, Baoyu Zhang, Li Huan, Peipei Zhao, Apeng Lin, Guangce Wang
BACKGROUND: Rising CO2 concentration was reported to increase phytoplankton growth rate as well as lipid productivity. This has raised questions regarding the NADPH supply for high lipid synthesis as well as rapid growth of algal cells. RESULTS: In this study, growth, lipid content, photosynthetic performance, the activity, and expression of key enzymes in Calvin cycle and oxidative pentose phosphate pathway (OPPP) were analyzed in the marine diatom Phaeodactylum tricornutum under three different CO2 concentrations (low CO2 (0...
2015: Biotechnology for Biofuels
Aiyou Huang, Lixia Liu, Chen Yang, Guangce Wang
BACKGROUND: Microalgae are potential sources of biofuels and high-value compounds. Mixotrophic conditions usually promote growth of microalgae. The pennate diatom Phaeodactylum tricornutum, with its short life cycle, completely sequenced genome, and ease of transformation, can be used as a model for studying carbon metabolism in microalgae. RESULTS: We compared the growth rate of P. tricornutum (IOCAS-001) under different conditions and labeled the cells using [(13)C]glycerol (GL)...
2015: Biotechnology for Biofuels
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