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https://read.qxmd.com/read/30761010/partial-amino-acid-metabolism-and-glutamine-synthesis-as-the-ammonia-defensive-strategies-during-aerial-exposure-in-chinese-loach-paramisgurnus-dabryanus
#1
Yun-Long Zhang, Guang-Yi Wang, Zi-Han Zhang, Yun-Yi Xie, Hui Jin, Zhao-Ran Dong
The Paramisgurnus dabryanus was exposed to air to assess the changes in plasma, liver and muscle free amino acid (FAA) contents. The FAA concentrations in plasma, liver and muscle of P. dabryanus were significantly affected by aerial exposure ( P < 0.05). After 12 h of aerial exposure, the plasma glutamate contents increased significantly ( P < 0.05) and reached peak value at 24 h of air exposure. With increasing air exposure time, the plasma alanine contents increased significantly and more dramatically than the control values ( P < 0...
2019: Frontiers in Physiology
https://read.qxmd.com/read/30671971/oxynoemacheilus-cemali-a-new-species-of-stone-loach-teleostei-nemacheilidae-from-the-%C3%A3-oruh-river-drainage-turkey
#2
Davut Turan, Cüneyt Kaya, Gökhan Kalayci, Esra Bayçelebi, İsmail Aksu
Oxynoemacheilus cemali sp. nov. is described from the Çoruh River drainage in the eastern Black Sea basin. One molecular marker (coI), 25 morphometric and 4 meristic characters were analysed. Oxynoemacheilus cemali is distinguished from O. kosswigi, O. banarescui, O. samanticus and O. angorae in the Black Sea basin by having a suborbital groove in males, an axillary lobe at the pelvic-fin base, no dorsal adipose crest on the caudal peduncle, a slightly-forked caudal fin and 7-15 dark grey dorsal saddles. Morever, Oxynoemacheilus cemali is distinguished by commonly having 9-15 irregularly-shaped dark-grey bars on the flank posterior to the dorsal-fin origin or, rarely having a mottled pattern or 4-6 irregularly shaped dark-grey bars on the flank posterior to the dorsal-fin origin...
January 23, 2019: Journal of Fish Biology
https://read.qxmd.com/read/30654137/mobilization-of-leukemic-cells-using-plerixafor-as-part-of-a-myeloablative-preparative-regimen-for-patients-with-aml-undergoing-allografting-assessment-of-safety-and-tolerability
#3
Fotios V Michelis, David W Hedley, Sonal Malhotra, Sue Chow, David Loach, Vikas Gupta, Dennis D Kim, John Kuruvilla, Jeffrey H Lipton, Auro Viswabandya, Hans A Messner
Allogeneic hematopoietic cell transplantation (HCT) is potentially curative for acute myeloid leukemia (AML), however a major cause for treatment failure is disease relapse. The purpose of this single center phase 1 study was to determine the safety and tolerability of administration of the CXCR4 inhibitor plerixafor (Mozobil, Sanofi Genzyme) along with myeloablative conditioning in patients with AML undergoing allogeneic HCT. The rationale was that plerixafor may mobilize leukemic stem cells making them more susceptible to the conditioning chemotherapy (registered at ClinicalTrials...
January 14, 2019: Biology of Blood and Marrow Transplantation
https://read.qxmd.com/read/30632094/impact-recovery-and-carryover-effect-of-roundup%C3%A2-on-predator-recognition-in-common-spiny-loach-lepidocephalichthys-thermalis
#4
Sandip D Tapkir, Sanjay S Kharat, Pradeep Kumkar, Sachin M Gosavi
Understanding the negative impact of a variety of environmental contaminants on aquatic animals is essential to curb biodiversity loss and stop degradation of ecological functions. Excessive and unrestricted use of pesticides is the most serious threat to aquatic animals including amphibians and fishes. Among the known pesticides, glyphosate based formulations have been shown to have lethal effects on many aquatic organisms. However, negative effects of pesticides on crucial ecological interactions such as prey-predator interactions are relatively unknown from tropics...
January 10, 2019: Ecotoxicology
https://read.qxmd.com/read/30599252/immunoglobulin-ig-heavy-chain-gene-locus-and-immune-responses-upon-parasitic-bacterial-and-fungal-infection-in-loach-misgurnus-anguillicaudatus
#5
Jie Xu, Yongyao Yu, Zhenyu Huang, Shuai Dong, Yanzhi Luo, Wei Yu, Yaxing Yin, Huili Li, Yangzhou Liu, Xiaoyun Zhou, Zhen Xu
Teleost fish are the most primitive bony vertebrates that contain immunoglobulin (Ig). Although teleost Ig is known to be important during tetrapod evolution and comparative immunology, little is known about the genomic organization of the immunoglobulin heavy-chain (IgH) locus. Here, three Ig isotype classes, IgM, IgD and IgT, were firstly identified in dojo loach (Misgurnus anguillicaudatus), and the IgH locus covering τ, μ and δ genes was also illustrated. Variable (V) gene segments lie upstream of two tandem diversity (D), joining (J) and constant (C) clusters and the genomic organization of the IgH locus presented as Vn -Dn -Jn -Cτ -Dn -Jn -Cμ -Cδ , similar to some other teleost fish...
December 29, 2018: Fish & Shellfish Immunology
https://read.qxmd.com/read/30594644/cloning-of-fatty-acid-binding-protein-2-fabp2-in-loach-misgurnus-anguillicaudatus-and-its-expression-in-response-to-dietary-oxidized-fish-oil
#6
Yin Zhang, Xiaojuan Cao, Jian Gao
Fatty acid-binding protein 2 (FABP2) is involved in the uptake of dietary fatty acids and intracellular fatty acid transport. In the present study, cDNA of fabp2 in loach (Misgurnus anguillicaudatus) was cloned and its full length was 956 bp, encoding 134 amino acids. Gene expression of fabp2 was investigated in different development stages and different tissues of loach, showing that the expression of fabp2 was recorded at 2 days after hatching (DAH), 10DAH, 20DAH and 35DAH, and higher in loach intestine, muscle and brain, compared with other tissues...
December 27, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30586807/microplastic-pollution-in-rice-fish-co-culture-system-a-report-of-three-farmland-stations-in-shanghai-china
#7
Weiwei Lv, Wenzong Zhou, Shibo Lu, Weiwei Huang, Quan Yuan, Minglu Tian, Weiguang Lv, Defu He
Microplastics are emerging contaminants of increasing concern. Despite the occurrence of microplastics in farmland soils, the knowledge on microplastics in rice-fish co-culture ecosystems is limited. In this study, we investigated the distribution of microplastics in three rice-fish culture stations in Shanghai. During non-rice and rice-planting periods, microplastics in water, soils and aquatic animals (eel, loach and crayfish) were systematically assayed using methods of NaCl density extraction, H2 O2 digestion and micro-fourier transform infrared spectroscopy...
February 20, 2019: Science of the Total Environment
https://read.qxmd.com/read/30485793/molecular-characterization-and-expression-analysis-of-t-cell-receptor-tcr-%C3%AE-and-%C3%AE-genes-in-dojo-loach-misgurnus-anguillicaudatus-in-response-to-bacterial-parasitic-and-fungal-challenge
#8
Yanzhi Luo, Wei Yu, Yongyao Yu, Shuai Dong, Yaxing Yin, Zhenyu Huang, Xinyu Wan, Liqiang Zhang, Yunzhen Yu, Taoshan Ai, Qingchao Wang, Zhen Xu
In mammalian, T-cell receptors (TCRs) play a key role in recognizing the presented antigen from external to protect organisms against environmental pathogens. To understand the potential roles of TCRγ and TCRδ in dojo loach (Misgurnus anguillicaudatus), Ma-TCRγ and Ma-TCRδ cDNAs were cloned and their gene expression profiles were investigated after bacterial, parasitic and fungal challenge. The open reading frame (ORF) of Ma-TCRγ and Ma-TCRδ cDNAs contained 948 and 867 bp, encoding 316 and 288 amino acid residues, respectively...
November 25, 2018: Fish & Shellfish Immunology
https://read.qxmd.com/read/30473287/relationship-between-heavy-metals-and-dissolved-organic-matter-released-from-sediment-by-bioturbation-bioirrigation
#9
Yi He, Bin Men, Xiaofang Yang, Yaxuan Li, Hui Xu, Dongsheng Wang
Organic matter (OM) is an important component of sediment. Bioturbation/bioirrigation can remobilize OM and heavy metals that were previously buried in the sediment. The remobilization of buried organic matter, thallium (Tl), cadmium (Cd), copper (Cu) and zinc (Zn) from sediment was studied in a laboratory experiment with three organisms: tubificid, chironomid larvae and loach. Results showed that bioturbation/bioirrigation promoted the release of dissolved organic matter (DOM) and dissolved Tl, Cd, Cu and Zn, but only dissolved Zn concentrations decreased with exposure time in overlying water...
January 2019: Journal of Environmental Sciences (China)
https://read.qxmd.com/read/30370880/intra-ooplasmic-injection-of-a-multiple-number-of-sperm-to-induce-androgenesis-and-polyploidy-in-the-dojo-loach-misgurnus-anguillicaudatus-teleostei-cobitidae
#10
George Shigueki Yasui, Taiju Saito, Yan Zhao, Takafumi Fujimoto, Etsuro Yamaha, Katsutoshi Arai
SummaryPolyspermy was initiated by microinjecting a multiple number of sperm into the activated and dechorionated eggs of dojo loach Misgurnus anguillicaudatus (Teleostei: Cobitidae). A 10-nl sperm suspension from an albino (recessive trait) male (105, 106, 107 or 108 sperm ml -1) was microinjected into eggs from a wild-type female. Although the rates of embryos developing into the blastula stage in the injection group at the highest sperm concentration were similar to that of the control group, the hatching rates of the injection group were much lower...
October 29, 2018: Zygote: the Biology of Gametes and Early Embryos
https://read.qxmd.com/read/30343118/molecular-characterization-and-expression-analysis-of-interleukin-15-il15-and-interleukin-15-receptor-subunit-alpha-il15r%C3%AE-in-dojo-loach-misgurnus-anguillicaudatus-their-salient-roles-during-bacterial-parasitic-and-fungal-infection
#11
Xiaoyao Chen, Weiguang Kong, Yongyao Yu, Shuai Dong, Zhenyu Huang, Wei Yu, Jie Xu, Yanzhi Luo, Qingchao Wang, Zhen Xu
Interleukin 15 (IL15) is a pleiotropic cytokine that participates in innate and adaptive immunity along with its receptor α-chain (IL15Rα). In order to investigate the potential roles of IL15 and IL15Rα in dojo loach (Misgurnus anguillicaudatus), we firstly cloned the cDNA sequence of Ma-IL15 and Ma-IL15Rα, which contain 1096bp and 1236bp and code proteins of 193 amino acids and 210 amino acids, respectively. A short signal peptide and Pfam IL15 domain were found in Ma-IL15, while a highly conserved sushi domain existed in Ma-IL15Rα...
November 2018: Molecular Immunology
https://read.qxmd.com/read/30313420/schistura-alboguttata-a-new-loach-species-of-the-family-nemacheilidae-pisces-cypriniformes-from-the-pearl-river-basin-in-guangxi-south-china
#12
Liang Cao, E Zhang
Schistura alboguttata, a new species of nemacheilid loach, is herein described from the Leli-He, a tributary flowing to the You-Jiang of the Pearl River basin (Zhu-Jiang in Chinese) at Tianlin County, Guangxi, South China. This new species can be readily distinguished from all other Chinese species of Schistura by its striking body coloration consisting of irregular white spots scattered over the dorsal and lateral regions of the body, with occasional irregular bars with narrow interspaces on the predorsal region...
September 4, 2018: Zootaxa
https://read.qxmd.com/read/30308027/diversity-of-spined-loaches-from-asia-minor-in-a-phylogenetic-context-teleostei-cobitidae
#13
Anabel Perdices, Cevher S Ozeren, Füsun Erkakan, Jörg Freyhof
Accurate determination of species diversity in areas of high endemicity, particularly those lacking comprehensive systematic knowledge, represents a challenge for both taxonomists and conservationists. This need is particularly evident in areas greatly affected by anthropogenic disturbances such as the Eastern Mediterranean and its freshwater environments. To improve our knowledge of Eastern Mediterranean freshwater fishes, we phylogenetically studied Western Palearctic Cobitis species, focusing on those found in Turkey...
2018: PloS One
https://read.qxmd.com/read/30240801/the-growth-performance-and-nonspecific-immunity-of-loach-paramisgurnus-dabryanus-as-affected-by-dietary-%C3%AE-1-3-glucan
#14
Ming Zhu, Shengjun Wu
Four diets supplemented with three different levels of β-1,3-glucan (0.5, 1, and 2 g kg-1 ) were formulated and tested for growth, intestine digestive-enzyme activities, PO, SOD and GPx activities and erythrocytes number, phagocytic percentage of erythrocytes and lysozyme activity and resistance to Aeromonas hydrophila of loach Paramisgurnus dabryanus. A diet without any β-1,3-glucan served as the control. After 60 days of feeding, the growth performance, intestine digestive-enzyme activities, nonspecific immunity, and resistance to Aeromonas hydrophila of P...
December 2018: Fish & Shellfish Immunology
https://read.qxmd.com/read/30189558/toxic-effects-of-copper-sulfate-on-diploid-and-triploid-fin-cell-lines-in-misgurnus-anguillicaudatus
#15
Yanjie Qin, Xia Li, Yanjin Yang, Zhuangzhuang Li, Yan Liang, Xiaoyu Zhang, Shan Jiang
The effects of different concentrations of copper sulfate on diploid and triploid fin cell lines (named DIMF and TRMF, respectively) in Misgurnus anguillicaudatus were studied. The LC50 of copper sulfate estimated by an MTT assay was 268.39 in DIMF cells, and 311.54 μmol/L in TRMF cells, respectively. Activity of superoxide dismutase (SOD) in DIMF cells gradually increased as the concentration of copper sulfate increased (up to 200 μmol/L), and then gradually decreased. SOD activity in triploid loach fin cells, as well as glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activity in both diploid and triploid cells, decreased as the concentration of copper sulfate increased, which suggested that excessive copper exposure at the concentrations tested in this study was detrimental to anti-oxidative capability...
December 1, 2018: Science of the Total Environment
https://read.qxmd.com/read/30153500/phylogeny-of-the-hillstream-loach-genus-mesonoemacheilus-reveals-widespread-diversification-through-ancient-drainage-connections-in-the-western-ghats-biodiversity-hotspot
#16
V K Anoop, Neelesh Dahanukar, Siby Philip, Liju Thomas, Rajeev Raghavan
Rivers draining the Western Ghats (WG) mountain ranges in peninsular India harbor an exceptionally diverse, unique and evolutionarily distinct assemblage of lower vertebrates with high levels of endemism, attributed to their evolution and potentially long history of isolation during the Late Cretaceous or Early Tertiary. A molecular phylogeny of hillstream loaches of the genus Mesonoemacheilus endemic to the WG revealed the presence of four clades which we designate as 'species groups'. A consensus of various species delimitation methods indicates the likelihood of 'at least' seven more undescribed species within Mesonoemacheilus...
December 2018: Molecular Phylogenetics and Evolution
https://read.qxmd.com/read/30112144/muscle-fatty-acid-content-in-selected-freshwater-fish-from-bukit-merah-reservoir-perak-malaysia
#17
Annette Jaya-Ram, Farhana Fuad, Mohd Shafiq Zakeyuddin, Amir Shah Ruddin Md Sah
One of the oldest reservoirs in Peninsular Malaysia, Bukit Merah Reservoir, is a place in which locals participate in fishing activities. Inland fisheries are important to individuals, society and the environment; whereby they generate a source of income and food security. It is essential to gauge the nutrition value of fish caught in this location as food source, especially in terms of fatty acid composition, to better demonstrate its potential towards the betterment of human health and general well-being...
July 2018: Tropical Life Sciences Research
https://read.qxmd.com/read/30099364/the-effect-of-biochar-on-the-mitigation-of-the-chiral-insecticide-fipronil-and-its-metabolites-burden-on-loach-misgurnus-anguillicaudatus
#18
Han Qu, Ruixue Ma, Fang Wang, Jing Gao, Peng Wang, Zhiqiang Zhou, Donghui Liu
In this work, the enantioselective toxicity, enrichment, and distribution of fipronil and its main metabolites (fipronil sulfone, fipronil sulfide, and fipronil desulfinyl) in loach (Misgurnus anguillicaudatus) were studied. The influence of maize-straw derived biochar on acute toxicity and bioaccumulation of contaminants were also investigated. The three main metabolites were more toxic to loach than parent fipronil. Meanwhile, loach exhibited more sensitive to S-enantiomer. The alleviated toxic response of loach was observed in the presence of biochar during 72-h acute toxicity test...
October 15, 2018: Journal of Hazardous Materials
https://read.qxmd.com/read/30076392/full-length-transcriptome-of-misgurnus-anguillicaudatus-provides-insights-into-evolution-of-genus-misgurnus
#19
Shaokui Yi, Xiaoyun Zhou, Jie Li, Manman Zhang, Shuangshuang Luo
Reconstruction and annotation of transcripts, particularly for a species without reference genome, plays a critical role in gene discovery, investigation of genomic signatures, and genome annotation in the pre-genomic era. This study generated 33,330 full-length transcripts of diploid M. anguillicaudatus using PacBio SMRT Sequencing. A total of 6,918 gene families were identified with two or more isoforms, and 26,683 complete ORFs with an average length of 1,497 bp were detected. Totally, 1,208 high-confidence lncRNAs were identified, and most of these appeared to be precursor transcripts of miRNAs or snoRNAs...
August 3, 2018: Scientific Reports
https://read.qxmd.com/read/30063289/employing-photoredox-catalysis-for-dna-encoded-chemistry-decarboxylative-alkylation-of-%C3%AE-amino-acids
#20
Dominik K Kölmel, Richard P Loach, Thomas Knauber, Mark E Flanagan
A new procedure for the photoredox-mediated conjugate addition of radicals that can be conveniently generated from α-amino acids to DNA-tagged Michael acceptors and styrenes is presented. This C(sp3 )-C(sp3 ) coupling tolerates a broad array of structurally diverse radical precursors, including all of the 20 proteinogenic amino acids. Importantly, this reaction proceeds under mild conditions and in DNA-compatible aqueous media. Furthermore, the presented reaction conditions are compatible with DNA, making this reaction platform well suited for the construction of DNA-encoded libraries...
July 31, 2018: ChemMedChem
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