Bianca de Jonckheere, Ferdinand Kollotzek, Patrick Münzer, Vanessa Göb, Melina Fischer, Kristina Mott, Cristina Coman, Nina Nicole Troppmair, Mailin-Christin Manke, Monika Zdanyte, Tobias Harm, Manuel Sigle, Dominik Kopczynski, Andrea Bileck, Christopher Gerner, Nils Hoffmann, David Heinzmann, Alice Assinger, Meinrad Gawaz, David Stegner, Harald Schulze, Oliver Borst, Robert Ahrends
During megakaryopoiesis, megakaryocytes (MK) undergo cellular morphological changes with strong modification of membrane composition and lipid signaling. Here we adopt a lipid-centric multiomics approach to create a quantitative map of the MK lipidome during maturation and proplatelet formation. Data reveal that MK differentiation is driven by an increased fatty acyl import and de novo lipid synthesis, resulting in an anionic membrane phenotype. Pharmacological perturbation of fatty acid import and phospholipid synthesis blocked membrane remodeling and directly reduced MK polyploidization and proplatelet formation resulting in thrombocytopenia...
September 2023: Nat Cardiovasc Res