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Caitlin E Dudley, Alexandra J Morell, Michael E Duffey, Sangita P Patel
PURPOSE: An adverse effect of amantadine, a drug used for Parkinson's disease, is corneal edema. While corneal endothelial cell loss is noted with amantadine toxicity, the reversibility of corneal edema suggests that amantadine affects active mechanisms regulating corneal hydration. Although mainly known as a NMDA receptor antagonist, amantadine is also a K+ -channel blocker. The purpose of this study was to investigate potential mechanisms of amantadine's toxic effects on corneal endothelium...
February 13, 2019: Experimental Eye Research
Riccardo Monterubbianesi, Mladen Bencun, Pierfrancesco Pagella, Anna Woloszyk, Giovanna Orsini, Thimios A Mitsiadis
Human teeth contain a variety of mesenchymal stem cell populations that could be used for cell-based regenerative therapies. However, the isolation and potential use of these cells in the clinics require the extraction of functional teeth, a process that may represent a significant barrier to such treatments. Fibroblasts are highly accessible and might represent a viable alternative to dental stem cells. We thus investigated and compared the in vitro differentiation potential of human dental pulp stem cells (hDPSCs), gingival fibroblasts (hGFs) and foreskin fibroblasts (hFFs)...
February 11, 2019: Scientific Reports
Xingmei Feng, Chenfei Wang, Zhifeng Gu, Jian Ni, Dan Huang, Guijuan Feng, Min Lian, Qi Lu, Yihua Song
Rosuvastatin is a synthetic statin of 3-hydroxy-methyl-3-glutamyl coenzyme A reductase inhibitor. It has pleiotropic characteristics including hepatic selectivity, minimal metabolism, inhibition of inflammation, and induction of osteoblast differentiation. In this study, dental pulp stem cells (DPSCs) were treated with lipopolysaccharide alone or with rosuvastatin. Then, we examined the accelerative effects of rosuvastatin on odontoblast differentiation and mineralized nodule formation by real-time polymerase chain reaction (RT-PCR), western blot, alizarin red S staining, and alkaline phosphatase staining...
February 2019: Cellular Reprogramming
Sachie Nakatani, Yousuke Taguchi, Hiroya Ueda, Yuko Ishida, Yoshikazu Morita, Ken Kato, Masahiro Wada, Kenji Kobata
It has been reported that the intake of milk basic protein (MBP) increases bone density by promoting bone formation and suppressing bone resorption. However, few studies have been done on MBP in cartilage, the tissue adjacent to bone. We therefore investigated the effect of MBP on a chondrocyte cell line, ATDC5. In a proliferative assay using the WST-1 method, the addition of 10, 100, and 1,000 µg/mL of MBP to ATDC5 cells significantly increased the cell number by about 1.2-, 1.5-, and 1.7-fold, respectively, compared with the control cells...
January 31, 2019: Journal of Dairy Science
Cheng-Gong Wang, Zhan Liao, Han Xiao, Hua Liu, Yi-He Hu, Qian-De Liao, Da Zhong
BACKGROUND: Osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is of much significance for bone formation, the imbalance of it would result in osteoporosis and other pathological bone defects. Increasing evidences showed that long non-coding RNAs (lncRNAs) and miRNAs played vital roles in the regulation of osteogenic differentiation. LncRNA KCNQ1OT1 was often regarded as an imprinted lncRNA and was related to tumor progression, while its function in osteogenic differentiation remained unclear...
January 28, 2019: Experimental and Molecular Pathology
Jian Wang, Xiaobo Qiu, Tianhua Xu, Zitong Sheng, Li Yao
BACKGROUND: Abnormal mineral metabolism in patients with chronic kidney disease (CKD) may lead to vascular calcification, which is markedly associated with adverse events, including ischemic cardiac diseases and all-cause cardiovascular mortality. Thus, preventing and treating vascular calcification play an important role in improving the prognosis of CKD patients. OBJECTIVES: To investigate the potential functions of sclerostin and low-density lipoprotein receptor-related protein 4 (Lrp4) in alleviating the β-glycerophosphate (β-GP)-induced vascular smooth muscle cell (VSMC) calcification, and the protective effect of Ginkgo biloba extract (GBE)...
January 30, 2019: Blood Purification
Pei-Lin Shao, Shun-Cheng Wu, Zih-Yin Lin, Mei-Ling Ho, Chung-Hwan Chen, Chau-Zen Wang
Simvastatin (SVS) promotes the osteogenic differentiation of mesenchymal stem cells (MSCs) and has been studied for MSC-based bone regeneration. However, the mechanism underlying SVS-induced osteogenesis is not well understood. We hypothesize that α5 integrin mediates SVS-induced osteogenic differentiation. Bone marrow MSCs (BMSCs) derived from BALB/C mice, referred to as D1 cells, were used. Alizarin red S (calcium deposition) and alkaline phosphatase (ALP) staining were used to evaluate SVS-induced osteogenesis of D1 cells...
January 24, 2019: International Journal of Molecular Sciences
Thanaphum Osathanon, Jeeranan Manokawinchoke, Noppadol Sa-Ard-Iam, Rangsini Mahanonda, Prasit Pavasant, Jaijam Suwanwela
OBJECTIVES: The present study aimed to investigate the expression of Notch signaling components during osteogenic differentiation in vitro and bone healing in vivo. In addition, the influence of Notch signaling on osteogenic differentiation of human bone-derived cells was examined. METHODS: Gene expression profiling of osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells in vitro (GSE80614) and bone healing period of murine tibial fracture in vivo (GSE99388) was downloaded from Gene Expression Omnibus database...
January 18, 2019: Archives of Oral Biology
Bin Ge, Hongrui Liu, Qianyu Liang, Lingling Shang, Ting Wang, Shaohua Ge
OBJECTIVE: To explore the effect of oxytocin (OT) on the proliferation, migration, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in vitro. DESIGN: PDLSCs were obtained by limiting dilution method. Immunofluorescence (IF), cell-counting kit-8 (CCK8), cell migration assay, Alizarin Red S staining, cetylpyridinium chloride (CPC) colorimetry, quantitative real-time polymerase chain reaction (qRT-PCR), and western blot analysis were used to examine the effect of OT on oxytocin receptor (OTR) expression, cell proliferation, migration and osteogenic differentiation of PDLSCs...
January 19, 2019: Archives of Oral Biology
Fengxi Liu, Xin Wang, Yun Yang, Rongrong Hu, Wenhao Wang, Yuliang Wang
BACKGROUND: Although the involvement of glycoprotein non-metastatic melanomal protein B (GPNMB) in regulating the odontogenic differentiation of human dental pulp stem cells (hDPCs) has been identified, the underlying mechanisms are largely unknown. The purpose of this study is to investigate the effects of miR-508-5p on the GPNMB expression and the odontogenic differentiation of hDPCs. METHODS: In this study, hDPCs were isolated and identified by flow cytometric analysis...
January 22, 2019: Stem Cell Research & Therapy
Wenzhen Lin, Li Gao, Wenxin Jiang, Chenguang Niu, Keyong Yuan, Xuchen Hu, Rui Ma, Zhengwei Huang
BACKGROUND: Extracellular matrix secretion and odontoblastic differentiation in human dental pulp stem cells (hDPSCs) are the cellular bases for reparative dentinogenesis. Osteomodulin (OMD) is a member of the small leucine-rich proteoglycan family distributed in the extracellular matrix but little is known about its role in osteo/odontogenic differentiation. The objective of this study was to investigate the role of OMD during osteo/odontoblastic differentiation of hDPSCs. METHODS: hDPSCs were selected using immune-magnetic beads and their capability of multi-differentiation was identified...
January 22, 2019: BMC Oral Health
Yin-Di Wu, Meng Li, Xuan Liao, Sheng-Hong Li, Jian-Xin Yan, Lei Fan, Wen-Li She, Jian-Xin Song, Hong-Wei Liu
Adipose‑derived stem cells (ADSCs) are mesenchymal stem cells that are often used in regenerative medicine. Maintaining ADSC viability is important, as this optimizes the curative effects of cell therapy. However, the optimal conditions for cell viability preservation remain unknown. The present study aimed to acquire a better protocol for ADSC storage by comparing the effects of various solutions and temperatures for ADSC preservation, in order to suggest the most effective methods of short‑term ADSC preservation for clinical use...
January 10, 2019: Molecular Medicine Reports
Yoshiaki Nakamura, Yoshifumi Nakane, Masaoki Tsudzuki
The blue-breasted quail (Coturnix chinensis), the smallest species of quail with short generation interval and excellent reproductive performance, is a potential avian research model. A normal series of skeletal development of avian embryos could be served as a reference standard in the fields of developmental biology and teratological testing as well as in the investigation of mutation with skeletal abnormalities and in the study of the molecular mechanisms of skeletal development through genome manipulation...
January 17, 2019: Animal Science Journal, Nihon Chikusan Gakkaihō
Yifeng Bian, Xiaojie Ma, Ruixia Wang, Hua Yuan, Ning Chen, Yifei Du
Epidemiological evidence suggests that diabetes mellitus (DM) is an important factor in promoting periodontitis. It not only affects the attachment of connective tissue but also causes loss of alveolar bone. Hence, there is an urgent need to find an effective treatment for DM-induced bone deficiency. This study aimed to investigate the effects of human amniotic mesenchymal stem cells (HAMSCs) on the proliferation and osteogenic differentiation of DM-induced human bone marrow mesenchymal stem cells (HBMSCs)...
January 2019: FEBS Open Bio
Xi Chen, Lei Yang, Dawei Ge, Weiwei Wang, Zhaowei Yin, Junwei Yan, Xiaojian Cao, Chunzhi Jiang, Shengnai Zheng, Bin Liang
The purpose of the present study was to identify the key long non-coding (lnc)RNAs in the occurrence and development of osteoporosis (OP) and to explore the associated molecular mechanism. First, the Gene Expression Omnibus (GEO) datasets, with key words 'osteoporosis' and 'HG-133A', were screened. RankProd R package was used to calculate the dysregulated lncRNAs in OP. Following this, bone marrow mesenchymal stem cells (BM-MSCs) harvested from 3-week-old Sprague Dawley rats were employed for detection of osteoblast differentiation...
January 2019: Experimental and Therapeutic Medicine
Emily M Brook, Adam S Tenforde, Elizabeth M Broad, Elizabeth G Matzkin, Heidi Y Yang, Jamie E Collins, Cheri A Blauwet
INTRODUCTION: The Female Athlete Triad (Triad) is a syndrome describing three interrelated conditions: low energy availability (LEA), menstrual dysfunction, and low bone mineral density (BMD). Relative Energy Deficiency in Sport (RED-S) expands the Triad to include multiple physiologic consequences of LEA in both sexes. The purpose of this study is to determine the prevalence of factors associated with the Triad/RED-S in an elite para athlete population. METHODS: Athletes were United States elite para athletes training to qualify for the 2016 or the 2018 Paralympic games...
January 15, 2019: Scandinavian Journal of Medicine & Science in Sports
Marla Cuppini, Kelly Cristine Zatta, Letícia Boldrin Mestieri, Fabiana Soares Grecca, Vicente Castelo Branco Leitune, Sílvia Stanisçuaski Guterres, Fabrício Mezzomo Collares
OBJECTIVE: The aim of this study was to synthesize and characterize an experimental endodontic paste. METHODS: An experimental endodontic paste (EX) was characterized by its particle size, zeta potential, drug content and morphology. The powder of EX is composed of amoxicillin microspheres, calcium tungstate and α-tricalcium phosphate, mixed with an indomethacin nanocapsules suspension. Ultracal® (Ultradent), an iodoform-based paste (GP) and the EX were evaluated by its physical properties (flow, film thickness and radiopacity)...
January 11, 2019: Dental Materials: Official Publication of the Academy of Dental Materials
Monireh Kouhi, Venugopal Jayarama Reddy, Mohammadhossein Fathi, Morteza Shamanian, Afsaneh Valipouri, Seeram Ramakrishna
Guided bone regeneration (GBR) has been established to be an effective method for the repair of defective tissues, which is based on isolating bone defects with a barrier membrane for faster tissue reconstruction. The aim of the present study is to develop poly (hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/fibrinogen (FG)/bredigite (BR) membranes with applicability in GBR. BR nanoparticles were synthesized through a sol-gel method and characterized using transmission electron microscopy (TEM) and X-ray diffractometer (XRD)...
January 12, 2019: Journal of Biomedical Materials Research. Part A
Yaoyao Fang, Zhiyuan Xue, Lianggong Zhao, Xiuyan Yang, Yafei Yang, Xianglin Zhou, Shilan Feng, Keming Chen
Calycosin has been reported to have a strong osteogenic activity and a positive correlation with anti-osteoporosis effects. However, its precise mechanism of action remains unclear. Since insulin-like growth factor 1 receptor (IGF1R) signaling and phosphatidylinositol 3-kinase/ Akt (PI3K/Akt) signaling have been shown to play a pivotal role in regulating osteogenesis, we hypothesized that the osteogenic activity of calycosin is mediated by these signaling pathways. Rat calvarial osteoblasts (ROBs) were cultured in osteogenic medium containing calycosin with or without GSK1904529A (GSK) or LY294002 (LY) (inhibitors of IGF1R and PI3K, respectively)...
January 11, 2019: Cell Biology International
Ben Desbrow, Nicholas A Burd, Mark Tarnopolsky, Daniel R Moore, Kirsty J Elliott-Sale
Adolescent, female and Masters athletes have unique nutritional requirements as a consequence of undertaking daily training and competition in addition to the specific demands of age and gender-related physiological changes. Dietary education and recommendations for these special population athletes require a focus on eating for long-term health, with special consideration given to "at risk" dietary patterns and nutrients (e.g. sustained restricted eating, low calcium, Vitamin D and/or iron intakes relative to requirements)...
January 11, 2019: International Journal of Sport Nutrition and Exercise Metabolism
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