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tumor chemosensitivity assay

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https://read.qxmd.com/read/30738960/expression-of-the-lncrna-zfas1-in-cervical-cancer-and-its-correlation-with-prognosis-and-chemosensitivity
#1
Lan-Lan Feng, Fang-Rong Shen, Jin-Hua Zhou, You-Guo Chen
OBJECTIVE: To investigate the expression of the lncRNA ZFAS1 in cervical cancer and its relationship with patient prognosis and cervical cancer cell chemosensitivity. METHODS: The expression of ZFAS1 in cervical cancer tissues and cell lines was detected by qRT-PCR. The cervical cancer CaSki and the HeLa cell lines were transfected to be divided into Blank, siR-Control, and siR-ZFAS1 groups. MTT, wound-healing, and transwell assays were used to evaluate cell biological function...
February 7, 2019: Gene
https://read.qxmd.com/read/30718314/adenosine-triphosphate-based-chemotherapy-response-assay-predicts-long-term-survival-of-primary-epithelial-ovarian-cancer
#2
Lan Ying Li, Sang Wun Kim, Eun Ji Nam, JungYun Lee, Sunghoon Kim, Young Tae Kim
OBJECTIVE: The aim of this study is to analyze the long-term relapse-free survival and overall survival outcomes of primary ovarian cancer patients using adenosine triphosphate-based chemotherapy response analysis. METHODS: In total, 162 primary epithelial ovarian cancer patients who underwent chemotherapy response assay for carboplatin, cisplatin, and paclitaxel by adenosine triphosphate-based chemotherapy response analysis prior to chemotherapy between December 2006 and November 2016 were retrospectively reviewed...
February 2019: International Journal of Gynecological Cancer
https://read.qxmd.com/read/30714183/zinc-promotes-prostate-cancer-cell-chemosensitivity-to-paclitaxel-by-inhibiting-epithelial-mesenchymal-transition-and-inducing-apoptosis
#3
Ya-Nan Xue, Bing-Bing Yu, Ya-Nan Liu, Rui Guo, Jiu-Ling Li, Li-Chao Zhang, Jing Su, Lian-Kun Sun, Yang Li
BACKGROUND: Paclitaxel (PTX) is a first-line chemotherapeutic drug for the treatment of prostate cancer. However, most patients develop resistance and metastasis, and thus new therapeutic approaches are urgently required. Recent studies have identified widespread anti-tumor effects of zinc (Zn) in various tumor cell lines, especially prostate cancer cells. In this study, we examined the effects of Zn as an adjuvant to PTX in prostate cancer cells. METHODS: PC3 and DU145 cells were treated with different concentrations of Zn and/or PTX...
February 3, 2019: Prostate
https://read.qxmd.com/read/30710750/microrna-137-inhibits-autophagy-and-chemosensitizes-pancreatic-cancer-cells-by-targeting-atg5
#4
Zhi-Chao Wang, Fei-Zhou Huang, Hong-Bo Xu, Ji-Chun Sun, Chang-Fa Wang
PURPOSE: Autophagy play an important role in tumor chemotherapy resistance. It has been reported that miR-137 expression was reducedand involved in the regulation of sensitivity of PC cells to chemotherapy. However, little is known about the underlying molecular mechanisms. In this study, we hypothesized that miR-137 might sensitize PC cells to chemotherapy thought regulating cell autophagy. METHODS: Cell survival was determined with MTT assay. Apoptotic cells were assessed with flow cytometric analysis...
January 30, 2019: International Journal of Biochemistry & Cell Biology
https://read.qxmd.com/read/30697056/scutellarin-inhibits-the-metastasis-and-cisplatin-resistance-in-glioma-cells
#5
Shi-Lei Tang, Yuan-Lin Gao, Wen-Zhong Hu
Background: Scutellarin is a natural flavone compound that possesses anti-tumor and chemosensitization effects in several cancers. However, the effects of scutellarin on metastasis and chemoresistance in glioma have not been illustrated. Methods: Glioma cells were treated with scutellarin in the presence or absence of LY294002. Cell proliferation was measured using a Cell Proliferation BrdU ELISA kit. Cell migration and invasion were analyzed using transwell assay...
2019: OncoTargets and Therapy
https://read.qxmd.com/read/30677405/microrna-31-triggers-g-2-m-cell-cycle-arrest-enhances-the-chemosensitivity-and-inhibits-migration-and-invasion-of-human-gastric-cancer-cells-by-downregulating-the-expression-of-zeste-homolog-2-zh2
#6
Ke-Kang Sun, Xiao-Jun Shen, Dong Yang, Ming-Qiang Gan, Gang Liu, Yi-Fan Zhang, Peng Hua, Hao-Dong Wang, Xiao-Yang Wu
Gastric cancer is the second most leading cause of cancer related mortality across the world over. Although the incidence of GC has declined to some extent but it is still the fourth highly diagnosed cancer across the world. GC generally remains undiagnosed till advanced stages due to unavailability of biomarkers and when diagnosed it becomes difficult to manage due to the lack of therapeutic targets and efficient chemotherapy. There are concrete evidences suggesting that miRNAs may prove important therapeutic targets for the treatment of devastating diseases such as cancer...
January 21, 2019: Archives of Biochemistry and Biophysics
https://read.qxmd.com/read/30659321/chemosensitization-of-hepatocellular-carcinoma-cells-to-sorafenib-by-%C3%AE-caryophyllene-oxide-induced-inhibition-of-abc-export-pumps
#7
Silvia Di Giacomo, Oscar Briz, Maria J Monte, Laura Sanchez-Vicente, Lorena Abete, Elisa Lozano, Gabriela Mazzanti, Antonella Di Sotto, Jose J G Marin
Several ATP-binding cassette (ABC) proteins reduce intracellular concentrations of antitumor drugs and hence weaken the response of cancer cells to chemotherapy. Accordingly, the inhibition of these export pumps constitutes a promising strategy to chemosensitize highly chemoresistant tumors, such as hepatocellular carcinoma (HCC). Here, we have investigated the ability of β-caryophyllene oxide (CRYO), a naturally occurring sesquiterpene component of many essential oils, to inhibit, at non-toxic doses, ABC pumps and improve the response of HCC cells to sorafenib...
January 18, 2019: Archives of Toxicology
https://read.qxmd.com/read/30655782/silencing-of-mir-19a-3p-enhances-osteosarcoma-cells-chemosensitivity-by-elevating-the-expression-of-tumor-suppressor-pten
#8
Bo Zhang, Yuan Liu, Jiangnan Zhang
MicroRNAs (miRNAs/miRs) are small non-coding RNAs, which serve important roles in tumor progression. The present study analyzed the role of miR-19a-3p in the chemosensitivity of osteosarcoma (OS) cells. Overexpression of miR-19a-3p was observed in OS cells and a cisplatin-resistant MG63 cell line was subsequently constructed. It was observed that miR-19a-3p inhibitor transfection suppressed cell proliferation and decreased the expression of Ki67 and PCNA compared with the cisplatin treatment group. miR-19a-3p inhibitor transfection also promoted apoptotic rate, increased the expression of Bcl-2 associated X, apoptosis regulator (Bax) and markedly decreased the expression of Bcl-2 compared with the cisplatin treatment group...
January 2019: Oncology Letters
https://read.qxmd.com/read/30655741/overexpression-of-ulk2-inhibits-proliferation-and-enhances-chemosensitivity-to-cisplatin-in-non-small-cell-lung-cancer
#9
Hong Cheng, Ze-Tian Yang, Yu-Quan Bai, Yan-Fei Cai, Jin-Ping Zhao
The aim of the present study was to examine the function of unc-51 like autophagy activating kinase 2 (Ulk2) in non-small cell lung cancer (NSCLC). Western blotting was used to analyze the protein expression of Ulk2 in seven pairs of cancerous and adjacent non-cancerous NSCLC specimens. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was used to determine the mRNA expression of Ulk2 in 20 pairs of tumor and adjacent normal tissues. Two NSCLC cell lines, A549 and H460, were transfected with an Ulk2 overexpression plasmid or empty vector; cell proliferation and chemosensitivity were measured using an MTT assay, and flow cytometry and western blotting were used to evaluate apoptosis...
January 2019: Oncology Letters
https://read.qxmd.com/read/30643424/evodiamine-inactivates-nf-%C3%AE%C2%BAb-and-potentiates-the-antitumor-effects-of-gemcitabine-on-tongue-cancer-both-in-vitro-and-in-vivo
#10
Qi Guo, Yanmei Liu, Jiayuan Zhao, Jing Wang, Yue Li, Yunqing Pang, Jian Chen, Jing Wang
Objective: The aim of this study was to investigate whether evodiamine (EVO) could potentiate the antitumor activity of gemcitabine (GEM) in tongue cancer cells and determine its potential underlying mechanisms. Materials and methods: Human Tca8113 and CAL-27 tongue squamous carcinoma cell lines were treated with EVO and GEM in different sequences and doses, after which cell proliferation was measured. Drug interactions were analyzed using the Chou-Talalay method with CompuSyn software...
2019: OncoTargets and Therapy
https://read.qxmd.com/read/30641875/ciprofloxacin-enhances-the-chemosensitivity-of-cancer-cells-to-abcb1-substrates
#11
Pranav Gupta, Hai-Ling Gao, Yunali V Ashar, Nishant M Karadkhelkar, Sabesan Yoganathan, Zhe-Sheng Chen
ABCB1 is one of the major drug efflux transporters that is known to cause multidrug resistance (MDR) in cancer patients receiving chemotherapy for the treatment of solid tumors and hematological malignancies. Inhibition of ABCB1 efflux function is important for maintaining the intracellular concentration of chemotherapeutic drugs. Here, we evaluated ciprofloxacin for its ability to reverse MDR caused by the overexpression of ABCB1. Cytotoxicity of ciprofloxacin was determined by the MTT assay. The chemosensitizing effects of ciprofloxacin were determined in combination with ABCB1 substrates...
January 11, 2019: International Journal of Molecular Sciences
https://read.qxmd.com/read/30638935/leptin-elicited-mirna-342-3p-potentiates-gemcitabine-resistance-in-pancreatic-ductal-adenocarcinoma
#12
Longyang Ma, Zhiyong Fan, Gongliang Du, Hui Wang
Although obesity (characterized by high levels of serum leptin) and deregulated expression of miRNAs are both functionally implicated in the pathogenesis of chemoresistance of pancreatic ductal adenocarcinoma (PDAC), the mechanistic link synchronize these two factors remain poorly understood. Here, we show that expression levels of obesity-associated miR-342-3p were significantly upregulated in gemcitabine (GEM)-resistant PDAC tissues and cells, and this upregulation was associated with poor postchemotherapy prognosis...
January 10, 2019: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/30636640/piwi-interacting-rna-36712-restrains-breast-cancer-progression-and-chemoresistance-by-interaction-with-sepw1-pseudogene-sepw1p-rna
#13
Liping Tan, Dongmei Mai, Bailin Zhang, Xiaobing Jiang, Jialiang Zhang, Ruihong Bai, Ying Ye, Mei Li, Ling Pan, Jiachun Su, Yanfen Zheng, Zexian Liu, Zhixiang Zuo, Qi Zhao, Xiaoxing Li, Xudong Huang, Jie Yang, Wen Tan, Jian Zheng, Dongxin Lin
BACKGROUND: Breast cancer is one of the most common malignancies and the major cause of cancer-related death in women. Although the importance of PIWI-interacting RNAs (piRNAs) in cancer has been increasingly recognized, few studies have been explored the functional mechanism of piRNAs in breast cancer development and progression. METHODS: We examined the top 20 highly expressed piRNAs based on the analysis of TCGA breast cancer data in two patient cohorts to test the roles of piRNAs in breast cancer...
January 12, 2019: Molecular Cancer
https://read.qxmd.com/read/30631898/upregulation-of-mta1-expression-by-human-papillomavirus-infection-promotes-cddp-resistance-in-cervical-cancer-cells-via-modulation-of-nf-%C3%AE%C2%BAb-apobec3b-cascade
#14
Qiu-Ping Jia, Chang-You Yan, Xue-Rong Zheng, Xia Pan, Xin Cao, Lei Cao
OBJECTIVE: Compelling evidence establishes the etiological role of viral proteins E6 and E7 of high-risk human papillomaviruses (HPV) in cervical carcinogenesis, but their contribution in chemoresistance that leads to advanced metastatic lesions remains poorly defined. Since metastasis-associated protein 1 (MTA1) upregulation and augmentation of APOBEC3B expression are both strongly associated with cervical cancer (CCa) development, and both molecules have been shown to be functionally associated with NF-κB pathway, we therefore sought to investigate the potential mechanistic link between MTA1, APOBEC3B and NF-κB during the pathogenesis of cisplatin (CDDP) resistance in HPV-positive CCa cells...
January 10, 2019: Cancer Chemotherapy and Pharmacology
https://read.qxmd.com/read/30629941/potentialization-of-anticancer-agents-by-identification-of-new-chemosensitizers-active-under-hypoxia
#15
Marx Sébastien, VAN Gysel Mégane, Breuer Aurélie, Dal Maso Thomas, Michiels Carine, Wouters Johan, L E Calve Benjamin
Hypoxia is one of the most important biological phenomena that influences cancer agressiveness and chemotherapy resistance. Cancer cells display dysregulated pathways notably resulting from oncogene expression. Tumors also show modifications in extracellular pH, extracellular matrix remodeling, neo-angiogenesis, hypoxia compared to normal tissues. Classically, the conventional anticancer therapies are efficient in cancer cells in normoxic conditions but under hypoxia, chemoresistance may occur. The addition of compounds that potentiate their activity in low oxygen environment could be a strategy to counteract this resistance...
January 7, 2019: Biochemical Pharmacology
https://read.qxmd.com/read/30626779/galectin-3-regulates-chemotherapy-sensitivity-in-epithelial-ovarian-carcinoma-via-regulating-mitochondrial-function
#16
Dawei Wang, Dong You, Lei Li
Galectin-3 (Gal-3) is a multifunctional carbohydrate-binding protein associated with cell migration, cell proliferation, cell adhesion, and cell-cell interaction in tumor cells. It has been implied to be involved in the tumor progression and chemoresistance of epithelial ovarian cancer (EOC). However, it is unclear whether the Gal-3-mediated regulation on the EOC chemosensitivity is associated with hypoxia or mitochondrial dysfunction. In the present study, we examined the regulation by Gal-3 overexpression on cisplatin-sensitivity or cisplatin-resistance in EOC cells in vitro...
2019: Journal of Toxicological Sciences
https://read.qxmd.com/read/30606601/amine-functionalized-porous-silica-coated-nayf-4-yb-er-upconversion-nanophosphors-for-efficient-delivery-of-doxorubicin-and-curcumin
#17
Kumbam Lingeshwar Reddy, Peeyush Kumar Sharma, Ashutosh Singh, Ajay Kumar, Konathala Ravi Shankar, Yashveer Singh, Neha Garg, Venkata Krishnan
Upconversion nanoparticles (UCNP) with unique multi-photon excitation photo-luminescence properties have been extensively explored as novel contrast agents for low-background biomedical imaging. There is an increasing interest in employing UCNPs as carrier for drug delivery as these offers a unique opportunity to combine therapy and diagnostics in one platform (theranostics). In the present work, we report microwave-assisted synthesis of hexagonal NaYF4 :Yb/Er UCNPs coated with porous silica and functionalized with amine (UCNP@mSiO2 )...
March 2019: Materials Science & Engineering. C, Materials for Biological Applications
https://read.qxmd.com/read/30594556/targeting-cancer-stem-cell-signature-gene-smoc-2-overcomes-chemoresistance-and-inhibits-cell-proliferation-of-endometrial-carcinoma
#18
Huan Lu, Dan-Dan Ju, Guang-Dong Yang, Lin-Yan Zhu, Xiao-Mei Yang, Jun Li, Wei-Wei Song, Jin-Hao Wang, Can-Can Zhang, Zhi-Gang Zhang, Rong Zhang
BACKGROUND: Endometrial cancer is one of the most common gynecological malignancies and has exhibited an increasing incidence rate in recent years. Cancer stem cells (CSCs), which are responsible for tumor growth and chemoresistance, have been confirmed in endometrial cancer. However, it is still challenging to identify endometrial cancer stem cells to then target for therapy. METHODS: Flow cytometry was used to identify the endometrial cancer stem cells. Sphere formation assay, western blotting, qRT-PCR assay, cell viability assay, xenograft assay and immunohistochemistry staining analysis were utilized to evaluate the effect of SPARC-related modular calcium binding 2 (SMOC-2) on the cells proliferation and drug resistance...
December 26, 2018: EBioMedicine
https://read.qxmd.com/read/30592329/knockdown-of-slc34a2-inhibits-cell-proliferation-metastasis-and-elevates-chemosensitivity-in-glioma
#19
Zhijun Bao, Lihua Chen, Shiwen Guo
Solute carrier 34 A2 (SLC34A2) is a member of SLC34 family that is a group of phosphate transporters. SLC34A2 has been reported to play critical roles in tumorigenesis and progression. However, the researches about the biological roles of SLC34A2 in glioma have not yet been reported. In this study, we analyzed the expression patterns of SLC34A2 in clinical glioma tumor tissues and cell lines. The results demonstrated that SLC34A2 was generally overexpressed in both glioma tissues and cell lines. To further investigate the roles of SLC34A2 in glioma, lentivirus containing specific SLC34A2 short hairpin RNA (sh-SLC34A2) was used to infect glioma cell lines U251 and U87 for the knockdown of SLC34A2...
December 28, 2018: Journal of Cellular Biochemistry
https://read.qxmd.com/read/30585767/adenovirus-mediated-crm197-sensitizes-human-glioma-cells-to-gemcitabine-by-the-mitochondrial-pathway
#20
Lv-Xia Dai, Jing Yang, Jian-Min Liu, Sizhou Huang, Bao-Ning Wang, Hong Li, Jie Yang, Zhong-Yi Zhao, Kang Cao, Ming-Yuan Li
PURPOSE: The cross-reacting material 197 (CRM197) is a mutation of the diphtheria toxin. The protein of CRM197 was used successfully for the therapy of various tumors in the recent studies. In this study, the recombinant adenoviruses containing the CRM197gene(AdCRM197) were used to enhance the cellar toxicity of gemcitabine in human glioma U87, U251, and H4 cells. PROCEDURES: MTT assay and flow cytometric analysis were performed to test the apoptosis of the U87, U251 and H4 cells with the combined treatment of AdCRM197 plus gemcitabine...
December 26, 2018: Cancer Biotherapy & Radiopharmaceuticals
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