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Yan He, Wei Wu, Shan Wu, Hui-Min Zheng, Pan Li, Hua-Fang Sheng, Mu-Xuan Chen, Zi-Hui Chen, Gui-Yuan Ji, Zhong-Dai-Xi Zheng, Prabhakar Mujagond, Xiao-Jiao Chen, Zu-Hua Rong, Peng Chen, Li-Yi Lyu, Xian Wang, Jia-Bao Xu, Chong-Bin Wu, Nan Yu, Yan-Jun Xu, Jia Yin, Jeroen Raes, Wen-Jun Ma, Hong-Wei Zhou
BACKGROUND: The metabolic syndrome (MetS) epidemic is associated with economic development, lifestyle transition and dysbiosis of gut microbiota, but these associations are rarely studied at the population scale. Here, we utilised the Guangdong Gut Microbiome Project (GGMP), the largest Eastern population-based gut microbiome dataset covering individuals with different economic statuses, to investigate the relationships between the gut microbiome and host physiology, diet, geography, physical activity and socioeconomic status...
September 24, 2018: Microbiome
J Benz, U Lempert, W Rüdiger
The incorporation of [1-(3)H] geranylgeranyl diphosphate (GGPP), [1-(3)H] geranylgeranyl monophosphate (GGMP) and [U-(14)C] phytyl diphosphate (PhPP) into chlorophylls a and b in growing tobacco cell cultures was investigated. The substrates were effectively incorporated into chlorophylls a and b, 3.2% of the total activity of applied GGPP or GGMP and 12.4% of the total activity of applied PhPP being found in chlorophylls a and b after 24 h incubation. The radioactivity was found in phytyl chlorophyllide through-out which means effective hydrogenation of the alcohol moiety in the case of GGPP and GGMP...
September 1984: Planta
Eun-Seob Cho, Hee-Dong Jeong
The cDNA of Crassostrea gigas HSP70 was cloned and rapid amplification of cDNA (RACE) techniques were used. The full length of HSP70 cDNA was 2045 bp, consisting of a 5' terminal untranslated region (UTR) of 80 bp, a 3' terminal UTR 146 bp, and an open reading frame (ORF) of 1829 bp encoding deduced 620 amino acids. The HSP70 cDNA contained HSP70 family signatures, ATP-GTP binding site motif, tetrapeptide (GGMP) and conserved carboxyl terminal region (EEVD) at C-terminal of deduced amino acid sequence. BLAST analysis revealed that the HSP70 gene has an extreme similarity of 98...
May 2012: Journal of Environmental Biology
Alexander Ioscovich, Angelika Fadeev, Alina Rivilis, Deborah Elstein
BACKGROUND: Epidural analgesia in older and multiparous women has been associated with risks. The aim of this study was to compare epidural analgesia use for labor/delivery in grand-grand multiparous women (GGMP; ≥10 births) relative to that in similar-aged women with lesser parity. METHODS: This was a prospective observational study of advanced age gravida. All laboring women in a six-month period admitted to a tertiary Israeli center were included if they were advanced age (≥36 years old) with one to two previous births (Low parity; n=128) or four to five previous births (Medium parity; n=181), and all GGMP (any age; n=187)...
November 2011: Journal of Perinatal Medicine
Angelica Fadeev, Alexander Ioscovitch, Alina Rivlis, Sorina Grisaru-Granovsky, Arnon Samueloff, Michael S Schimmel, Deborah Elstein
PURPOSE: Evaluation of outcome of labor/delivery in great-grand multiparous (GGMP) women relative to women with lower parity is complicated because of confounding parity with (older) age. Herein, we compare maternal and neonatal outcome in GGMP from a large obstetrics department to that in older women with lesser parity. METHODS: This was a prospective observational study of older gravid women. All laboring women in a 6-month period were included in this study if they were older than 36 years and had had 2-3 previous births (low parity) or 5-6 previous births (medium parity), and also all women ≥10 births (of any age); all other laboring women were excluded...
October 2011: Archives of Gynecology and Obstetrics
Ariel Marks, Jennifer Greenstein, Miriam T Berger, Joel Shapiro, Deborah Elstein, Alexander Ioscovich
Grand-grand multiparous (GGMP) women, that is women with ≥10 births, appear less likely to receive epidural anesthesia for labor/delivery than non-GGMP women. Eighteen months' data were collected. 277/16,331 laboring females were GGMP (mean age: 39.6 ± 3.3 years; mean: 11.3 ± 1.5 births), 83/258 (32.2%) delivered vaginally with epidural anesthesia versus 7,203/15,711 (45.8%) non-GGMP with epidural anesthesia (p < .0051; Odds Ratio = 0.56). Among GGMP women, 23/42 cesarean sections (55%) were emergency versus 1,011/1,631 (62%) among non-GGMP women (Odds Ratio: 0...
October 2010: Health Care for Women International
Tim Eaton
Seventy percent isopropyl alcohol (70% IPA) in water for injection is extensively utilised within pharmaceutical cleanrooms for glove and surface disinfection. When supplied in pressurised containers and delivered as an aerosol, it has been demonstrated that large quantities of 70% IPA particles are generated that remain airborne for substantial periods of time. Within non-unidirectional airflow cleanroom areas, such particles are likely to be recorded by the particle monitoring system. Consequently, the derived operational limits for particles will almost certainly be at "artificially high" levels and any particle generating activities with contamination potential may be masked...
November 2009: PDA Journal of Pharmaceutical Science and Technology
Zhaoxia Cui, Yuan Liu, Weisha Luan, Qianqian Li, Danhua Wu, Shuangyan Wang
Hsp70 can stimulate cells of the innate immune system directly by acting as "danger"-signaling molecules. To understand the immune defense mechanisms of swimming crab Portunus trituberculatus (Decapoda: Brachyura: Portunidae), the cDNA of Hsp70 (designated PtHsp70) was cloned by the combination of homology cloning and rapid amplification of cDNA ends (RACE). The full-length PtHsp70 cDNA was 2195 bp, including an open reading frame (ORF) of 1950 bp encoding a polypeptide of 650 amino acids with estimated molecular mass of 71...
January 2010: Fish & Shellfish Immunology
M Amor-Mahjoub, N Gomez-Vrielyunck, J P Suppini, B Fouchaq, N Benarouj, M Ladjimi
HSP70 from bacteria to man are known to self-associate to form multiple species suggesting that self-association is related to function. In order to determine the structural basis of HSP70 oligomerization, deletion mutants in the C-terminal domain of HSC70, a constitutive member of the HSP70 family, have been constructed and analyzed for their self-association properties by gel electrophoresis, size-exclusion chromatography and analytical ultracentrifugation. The results of this investigation indicate that, whereas deletion of the GGMP rich C-terminal extremity of HSC70, containing EEVD motif stabilizes the oligomeric species, deletions of either the aD-aE C-terminal helices or the inter-domain hydrophobic linker contribute to the stabilization of the monomeric form...
2006: Archives de L'Institut Pasteur de Tunis
R Wu, L Sun, M Lei, S T Xie
Heat shock protein 70s (HSP70s) are fundamental chaperone proteins that are indispensable to most living organisms. In order to investigate the function of HSP70 and heat shock response in shrimp, a heat shock cognate (HSC70) gene of the white shrimp (Litopenaeus vannamei), containing a 1959-bp open reading frame, was cloned and characterized. The amino acid sequence, 71.5 kDa of molecular weight, shares 80-99.6% homology with 12 diverse species' HSP70s and HSC70s. In fact, some segments of the eukaryotic HSC70 sequence, such as ATP/GTP-binding site, cytoplasmic HSP70 C-terminal sequence, and GGMP/GAP repeats, are also found in the putative shrimp HSC70...
March 2008: Molekuliarnaia Biologiia
Sven Lyrenäs
OBJECTIVE: To assess delivery time and the frequency of dystocia in grand multiparous (GMP) and grand grand multiparous (GGMP) women. METHOD: Labor records from 272 women with parity 4-8 (GMP) and 56 women with parity 9 or higher (GGMP) were retrospectively reviewed regarding duration of labor and the occurrence of dystocia. As a comparison, data from 263 women with parity 1-3 (LMP) and 87 nulliparas (NP) was used. RESULTS: Duration of labor, defined as time from admission of the laboring woman to delivery of the infant, lasted in median 2...
2002: Gynecologic and Obstetric Investigation
M A Webb, J M Cavaletto, P Klanrit, G A Thompson
The Hsp70-interacting protein Hip binds to the adenosine triphosphatase domain of Hsp70, stabilizing it in the adenosine 5'-diphosphate-ligated conformation and promoting binding of target polypeptides. In mammalian cells, Hip is a component of the cytoplasmic chaperone heterocomplex that regulates signal transduction via interaction with hormone receptors and protein kinases. Analysis of the complete genome sequence of the model flowering plant Arabidopsis thaliana revealed 2 genes encoding Hip orthologs. The deduced sequence of AtHip-1 consists of 441 amino acid residues and is 42% identical to human Hip...
July 2001: Cell Stress & Chaperones
E A Nollen, A E Kabakov, J F Brunsting, B Kanon, J Höhfeld, H H Kampinga
The chaperone activity of Hsp70 is influenced by the activities of both positive and negative regulatory proteins. In this study, we provide first time evidence for the stimulating effect of the Hsp70-interacting protein Hip on the chaperone activity in the mammalian cytosol. Overexpressing Hip enhances the refolding of the heat-inactivated reporter enzyme luciferase expressed in hamster lung fibroblasts. Also, it protects luciferase from irreversible denaturation under conditions of ATP depletion. We demonstrate that these stimulating actions depend on both the presence of the central Hsp70-binding site and the amino-terminal homo-oligomerization domain of Hip...
February 16, 2001: Journal of Biological Chemistry
S M Jayasena, N V Chandrasekharan, E H Karunanayake
The filarial parasite Setaria digitata is the causative agent of cerebrospinal nematodiasis in its abnormal hosts such as sheep, goats and horses, and therefore is of significant veterinary importance. Since very little is currently known about the biology of this parasite at molecular level, we have cloned and characterised a hsp70 gene, the first gene to be reported from this parasite. The genomic clone isolated contained sequences from two hsp70 genes. One gene, hsp70-2, was completely sequenced and found to contain nine introns ranging in size from 78 to 195 bp...
April 1999: International Journal for Parasitology
N Kumar, H Zheng
We have studied the immunogenicity of a recombinant heat-shock protein-related stress protein of 70,000 MW (Pfhsp) of the human malaria parasite Plasmodium falciparum in H-2 congenic and non-congenic strains of inbred and outbred mice. Most mice of different genetic backgrounds produced antibodies to Pfhsp, indicating a lack of any apparent genetic restriction of immune response. A peptide corresponding to the GGMP repeat sequence in the C-terminal region of Pfhsp was recognized by more than 75% of sera from immunized mice...
May 1998: Immunology
V Prapapanich, S Chen, D F Smith
Steroid receptor complexes are assembled through an ordered, multistep pathway involving multiple components of the cytoplasmic chaperone machinery. Two of these components are Hsp70-binding proteins, Hip and Hop, that have some limited homology in their C-terminal regions, outside the sequences mapped for Hsp70 binding. Within this region of Hip is a DPEV sequence that occurs twice; in Hop, one DPEV sequence plus a partial second sequence occurs. In an effort to better understand Hip function as it relates to assembly of progesterone receptor complexes, the DPEV region of Hip was targeted for mutations...
February 1998: Molecular and Cellular Biology
M F Wiser, G J Jennings, P Uparanukraw, A van Belkum, L J van Doorn, N Kumar
Molecular chaperones are important for proper protein folding during protein biogenesis. This report describes a protein from Plasmodium berghei which is 30% identical and 40% similar to a recently described mammalian cochaperone, or heat shock protein 70 interacting protein. The P. berghei cochaperone accumulates throughout the trophozoite stage and decreases during the schizont stage. The stage specific expression is consistent with its presumed role in protein folding or protein-protein interactions. The largest difference between the Plasmodium and mammalian sequences is a more extensive domain of imperfect glycine-glycine-methionine-proline (GGMP) tandem repeats in the parasite's cochaperone sequence...
December 2, 1996: Molecular and Biochemical Parasitology
J Y Fan, E A Davidson
We have isolated a 70-kD heat-shock protein (hsp-70) cDNA from Plasmodium berghei. A cDNA clone encoding the P. berghei hsp-70 was isolated and sequenced, demonstrating that it is highly homologous with other Plasmodium hsp-70s. One of the common features is a series of GGMP amino acid repeats at the carboxy terminus; there is also a long, AT-rich 5' untranslated region, a hallmark of other malarial RNAs. Hydropathy and antigenicity analyses suggest the presence of two hydrophilic domains. Recombinant peptides comprising different fragments of hsp-70 were expressed in Escherichia coli and assessed for antigenicity with antiserum from mice immunized with sonicated extracts of P...
November 1996: American Journal of Tropical Medicine and Hygiene
V Prapapanich, S Chen, E J Toran, R A Rimerman, D F Smith
The hsp70-interacting protein Hip participates in the assembly pathway for progesterone receptor complexes. During assembly, Hip appears at early assembly stages in a transient manner that parallels hsp70 interactions. In this study, a cDNA for human Hip was used to develop various mutant Hip forms in the initial mapping of functions to particular Hip structural elements. Hip regions targeted for deletion and/or truncation included the C-terminal region (which has some limited homology with Saccharomyces cerevisiae Sti1 and its vertebrate homolog p60), a glycine-glycine-methionine-proline (GGMP) tandem repeat, and a tetratricopeptide repeat (TPR)...
November 1996: Molecular and Cellular Biology
G Langsley, J C Barale, D Mattei
No abstract text is available yet for this article.
June 1993: Molecular and Biochemical Parasitology
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