keyword
https://read.qxmd.com/read/38537036/characterisation-of-the-merozoite-thrombospondin-related-anonymous-protein-mtrap-of-plasmodium-berghei-as-a-transmission-blocking-antigen
#1
JOURNAL ARTICLE
Xiomara Alexandra Gaitán, Juliana Calit, Irina Dobrescu, Marisé Solórzano Ramos, Alba Marina Gimenez, Daniel Youssef Bargieri
BACKGROUND: Malaria is an infectious disease caused by protozoan parasites belonging to the genus Plasmodium. Human-to-human transmission depends on a mosquito vector; thus, the interruption of parasite transmission from humans to mosquitoes is an important approach in the fight against malaria. The parasite stages infectious to mosquitoes are the gametocytes, sexual stages that are ingested by the vector during a blood meal and transform into male and female gametes in the midgut. Immunity against sexual stage antigens expressed by gametocytes, gametes, and the zygote formed after fertilisation can interrupt the parasite sexual cycle in the mosquito...
2024: Memórias do Instituto Oswaldo Cruz
https://read.qxmd.com/read/38504288/mir-430-regulates-zygotic-mrna-during-zebrafish-embryogenesis
#2
JOURNAL ARTICLE
Danielson Baia Amaral, Rhonda Egidy, Anoja Perera, Ariel A Bazzini
BACKGROUND: Early embryonic developmental programs are guided by the coordinated interplay between maternally inherited and zygotically manufactured RNAs and proteins. Although these processes happen concomitantly and affecting gene function during this period is bound to affect both pools of mRNAs, it has been challenging to study their expression dynamics separately. RESULTS: By employing SLAM-seq, a nascent mRNA labeling transcriptomic approach, in a developmental time series we observe that over half of the early zebrafish embryo transcriptome consists of maternal-zygotic genes, emphasizing their pivotal role in early embryogenesis...
March 19, 2024: Genome Biology
https://read.qxmd.com/read/38498454/plant-regeneration-via-adventitious-shoot-formation-from-immature-zygotic-embryo-explants-of-camelina
#3
JOURNAL ARTICLE
Barno Ruzimurodovna Rezaeva, Twan Rutten, Carola Bollmann, Stefan Ortleb, Michael Melzer, Jochen Kumlehn
Camelina is an oil seed crop that is enjoying increasing interest because it has a particularly valuable fatty acid profile, is modest regarding its water and nutrient requirements, and is comparatively resilient to abiotic and biotic stress factors. The regeneration of plants from cells accessible to genetic manipulation is an essential prerequisite for the generation of genetically engineered plants, be it by transgenesis or genome editing. Here, immature embryos were used on the assumption that their incomplete differentiation was associated with totipotency...
February 6, 2024: Plants (Basel, Switzerland)
https://read.qxmd.com/read/38494256/the-role-of-zygotic-genome-activation-in-genetic-related-reproductive-medicine-technological-perspective-religious-and-bioethical-concerns-challenges-and-benefits
#4
JOURNAL ARTICLE
Nameer Hashim Qasim, Abzal Zhumagaliuly, Rabiga Khozhamkul, Fakher Rahim
Zygotic Genome Activation (ZGA) is a crucial developmental milestone in early embryogenesis, marking the transition from maternal to embryonic control of development. This process, which varies in timing across species, involves the activation of the embryonic genome, paving the way for subsequent cell differentiation and organismal development. Recent advances in genomics and reproductive medicine have highlighted the potential of ZGA in the realm of genetic screening, providing a window into the genetic integrity of the developing embryo at its earliest stages...
March 2024: Journal, Genetic Engineering & Biotechnology
https://read.qxmd.com/read/38493496/chromatin-modifier-ep400-regulates-oocyte-quality-and-zygotic-genome-activation-in-mice
#5
JOURNAL ARTICLE
Qing Tian, Ying Yin, Yu Tian, Yufan Wang, Yong-Feng Wang, Rikiro Fukunaga, Toshihiro Fujii, Ai-Hua Liao, Lei Li, Wei Zhang, Ximiao He, Wenpei Xiang, Li-Quan Zhou
Epigenetic modifiers that accumulate in oocytes, play a crucial role in steering the developmental program of cleavage embryos and initiating life. However, the identification of key maternal epigenetic regulators remains elusive. In the findings, the essential role of maternal Ep400, a chaperone for H3.3, in oocyte quality and early embryo development in mice is highlighted. Depletion of Ep400 in oocytes resulted in a decline in oocyte quality and abnormalities in fertilization. Preimplantation embryos lacking maternal Ep400 exhibited reduced major zygotic genome activation (ZGA) and experienced developmental arrest at the 2-to-4-cell stage...
March 17, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://read.qxmd.com/read/38493164/temporospatial-hierarchy-and-allele-specific-expression-of-zygotic-genome-activation-revealed-by-distant-interspecific-urochordate-hybrids
#6
JOURNAL ARTICLE
Jiankai Wei, Wei Zhang, An Jiang, Hongzhe Peng, Quanyong Zhang, Yuting Li, Jianqing Bi, Linting Wang, Penghui Liu, Jing Wang, Yonghang Ge, Liya Zhang, Haiyan Yu, Lei Li, Shi Wang, Liang Leng, Kai Chen, Bo Dong
Zygotic genome activation (ZGA) is a universal process in early embryogenesis of metazoan, when the quiescent zygotic nucleus initiates global transcription. However, the mechanisms related to massive genome activation and allele-specific expression (ASE) remain not well understood. Here, we develop hybrids from two deeply diverged (120 Mya) ascidian species to symmetrically document the dynamics of ZGA. We identify two coordinated ZGA waves represent early developmental and housekeeping gene reactivation, respectively...
March 16, 2024: Nature Communications
https://read.qxmd.com/read/38485817/programmed-cell-death-1-modified-pig-developed-using-electroporation-mediated-gene-editing-for-in-vitro-fertilized-zygotes
#7
JOURNAL ARTICLE
Thanh-Van Nguyen, Lanh Thi Kim Do, Qingyi Lin, Megumi Nagahara, Zhao Namula, Manita Wittayarat, Maki Hirata, Takeshige Otoi, Fuminori Tanihara
Programmed cell death-1 (PD-1) is an immunoinhibitory receptor required to suppress inappropriate immune responses such as autoimmunity. Immune checkpoint antibodies that augment the PD-1 pathway lead to immune-related adverse events (irAEs), organ non-specific side effects due to autoimmune activation in humans. In this study, we generated a PD-1 mutant pig using electroporation-mediated introduction of the CRISPR/Cas9 system into porcine zygotes to evaluate the PD-1 gene deficiency phenotype. We optimized the efficient guide RNAs (gRNAs) targeting PD-1 in zygotes and transferred electroporated embryos with the optimized gRNAs and Cas9 into recipient gilts...
March 14, 2024: In Vitro Cellular & Developmental Biology. Animal
https://read.qxmd.com/read/38479840/adnp-modulates-sine-b2-derived-ctcf-binding-sites-during-blastocyst-formation-in-mice
#8
JOURNAL ARTICLE
Wen Wang, Rui Gao, Dongxu Yang, Mingli Ma, Ruge Zang, Xiangxiu Wang, Chuan Chen, Xiaochen Kou, Yanhong Zhao, Jiayu Chen, Xuelian Liu, Jiaxu Lu, Ben Xu, Juntao Liu, Yanxin Huang, Chaoqun Chen, Hong Wang, Shaorong Gao, Yong Zhang, Yawei Gao
CTCF is crucial for chromatin structure and transcription regulation in early embryonic development. However, the kinetics of CTCF chromatin occupation in preimplantation embryos have remained unclear. In this study, we used CUT&RUN technology to investigate CTCF occupancy in mouse preimplantation development. Our findings revealed that CTCF begins binding to the genome prior to zygotic genome activation (ZGA), with a preference for CTCF-anchored chromatin loops. Although the majority of CTCF occupancy is consistently maintained, we identified a specific set of binding sites enriched in the mouse-specific short interspersed element (SINE) family B2 that are restricted to the cleavage stages...
March 13, 2024: Genes & Development
https://read.qxmd.com/read/38466753/a-maternal-to-zygotic-transition-gene-block-on-the-zebrafish-sex-chromosome
#9
JOURNAL ARTICLE
Catherine A Wilson, John H Postlethwait
Wild zebrafish (Danio rerio) have a ZZ/ZW chromosomal sex determination system with the major sex locus on the right arm of chromosome-4 (Chr4R) near the largest heterochromatic block in the genome, suggesting that Chr4R transcriptomics might differ from the rest of the genome. To test this hypothesis, we conducted an RNA-seq analysis of adult ZW ovaries and ZZ testes in the Nadia strain and identified four regions of Chr4 with different gene expression profiles. Unique in the genome, protein-coding genes in a 41...
March 11, 2024: G3: Genes—Genomes—Genetics
https://read.qxmd.com/read/38458200/imprinted-x-chromosome-inactivation-at-the-gamete-to-embryo-transition
#10
JOURNAL ARTICLE
Chunyao Wei, Barry Kesner, Hao Yin, Jeannie T Lee
In mammals, dosage compensation involves two parallel processes: (1) X inactivation, which equalizes X chromosome dosage between males and females, and (2) X hyperactivation, which upregulates the active X for X-autosome balance. The field currently favors models whereby dosage compensation initiates "de novo" during mouse development. Here, we develop "So-Smart-seq" to revisit the question and interrogate a comprehensive transcriptome including noncoding genes and repeats in mice. Intriguingly, de novo silencing pertains only to a subset of Xp genes...
February 29, 2024: Molecular Cell
https://read.qxmd.com/read/38457854/neddylation-inhibition-affects-early-embryonic-development-by-disrupting-maternal-to-zygotic-transition-and-mitochondrial-function-in-mice
#11
JOURNAL ARTICLE
Mingxiao Liu, Zhiming Ding, Peihao Sun, Shuo Zhou, Hanxiao Wu, Lijun Huo, Liguo Yang, John S Davis, Aixin Liang
Post-translational modifications (PTMs) are critical for early development in mice because early cleavage-stage embryos are characterized by transcriptional inactivity. Neddylation is an important ubiquitin-like PTM that regulates multiple biophysical processes. However, the exact roles of neddylation in regulating early embryonic development remain largely unknown. In the present study, we found that inhibition of neddylation by specific inhibitor MLN4924 led to severe arrest of early embryonic development...
February 28, 2024: Theriogenology
https://read.qxmd.com/read/38453481/a-maternal-effect-padi6-variant-causes-nuclear-and-cytoplasmic-abnormalities-in-oocytes-as-well-as-failure-of-epigenetic-reprogramming-and-zygotic-genome-activation-in-embryos
#12
JOURNAL ARTICLE
Carlo Giaccari, Francesco Cecere, Lucia Argenziano, Angela Pagano, Antonio Galvao, Dario Acampora, Gianna Rossi, Bruno Hay Mele, Basilia Acurzio, Scott Coonrod, Maria Vittoria Cubellis, Flavia Cerrato, Simon Andrews, Sandra Cecconi, Gavin Kelsey, Andrea Riccio
Maternal inactivation of genes encoding components of the subcortical maternal complex (SCMC) and its associated member, PADI6, generally results in early embryo lethality. In humans, SCMC gene variants were found in the healthy mothers of children affected by multilocus imprinting disturbances (MLID). However, how the SCMC controls the DNA methylation required to regulate imprinting remains poorly defined. We generated a mouse line carrying a Padi6 missense variant that was identified in a family with Beckwith-Wiedemann syndrome and MLID...
March 7, 2024: Genes & Development
https://read.qxmd.com/read/38444829/maternal-vgll4a-regulates-zebrafish-epiboly-through-yap1-activity
#13
JOURNAL ARTICLE
Carlos Camacho-Macorra, Noemí Tabanera, Elena Sánchez-Bustamante, Paola Bovolenta, Marcos J Cardozo
Gastrulation in zebrafish embryos commences with the morphogenetic rearrangement of blastodermal cells, which undergo a coordinated spreading from the animal pole to wrap around the egg at the vegetal pole. This rearrangement, known as epiboly, relies on the orchestrated activity of maternal transcripts present in the egg, compensating for the gradual activation of the zygotic genome. Epiboly involves the mechano-transducer activity of yap1 but what are the regulators of yap1 activity and whether these are maternally or zygotically derived remain elusive...
2024: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/38436556/polarity-establishment-in-the-plant-zygote-at-a-glance
#14
JOURNAL ARTICLE
Hikari Matsumoto, Minako Ueda
The complex structures of multicellular organisms originate from a unicellular zygote. In most angiosperms, including Arabidopsis thaliana, the zygote is distinctly polar and divides asymmetrically to produce an apical cell, which generates the aboveground part of the plant body, and a basal cell, which generates the root tip and extraembryonic suspensor. Thus, zygote polarity is pivotal for establishing the apical-basal axis running from the shoot apex to the root tip of the plant body. The molecular mechanisms and spatiotemporal dynamics behind zygote polarization remain elusive...
March 1, 2024: Journal of Cell Science
https://read.qxmd.com/read/38425578/pronuclear-transfer-rescues-poor-embryo-development-of-in-vitro-grown-secondary-mouse-follicles
#15
JOURNAL ARTICLE
Antonia Christodoulaki, Haitang He, Min Zhou, Chloë De Roo, Machteld Baetens, Tine De Pretre, Muhammad Fakhar-I-Adil, Björn Menten, Ann Van Soom, Dominic Stoop, Annekatrien Boel, Björn Heindryckx
STUDY QUESTION: Is pronuclear transfer (PNT) capable of restoring embryo developmental arrest caused by cytoplasmic inferiority of in vitro -grown (IVG) mouse oocytes? SUMMARY ANSWER: PNT to in vivo matured cytoplasm significantly improved embryo development of IVG mouse oocytes, leading to living, fertile offspring. WHAT IS KNOWN ALREADY: In vitro follicle culture has been considered as a fertility preservation option for cancer patients...
2024: Human Reproduction Open
https://read.qxmd.com/read/38419548/rna-helicase-ddx5-is-involved-in-r-loop-dynamics-of-preimplantation-embryos
#16
JOURNAL ARTICLE
Hyeonji Lee, Dong Wook Han, Seonho Yoo, Ohbeom Kwon, Hyeonwoo La, Chanhyeok Park, Heeji Lee, Kiye Kang, Sang Jun Uhm, Hyuk Song, Jeong Tae Do, Youngsok Choi, Kwonho Hong
OBJECTIVE: R-loops are DNA:RNA triplex hybrids, and their metabolism is tightly regulated by transcriptional regulation, DNA damage response, and chromatin structure dynamics. R-loop homeostasis is dynamically regulated and closely associated with gene transcription in mouse zygotes. However, the factors responsible for regulating these dynamic changes in the R-loops of fertilized mouse eggs have not yet been investigated. This study examined the functions of candidate factors that interact with R-loops during zygotic gene activation...
February 22, 2024: Animal bioscience
https://read.qxmd.com/read/38411954/neddylation-is-essential-for-malaria-transmission-in-plasmodium-berghei
#17
JOURNAL ARTICLE
Bandita Nayak, Plabita Paul, Satish Mishra
Neddylation is a type of posttranslational modification known to regulate a wide range of cellular processes by covalently conjugating the ubiquitin-like protein Nedd8 to target proteins at lysine residues. However, the role of neddylation in malaria parasites has not been determined. Here, for the first time, we showed that neddylation plays an essential role in malaria transmission in Plasmodium berghei . We found that disruption of Nedd8 did not affect blood-stage propagation, gametocyte development, gamete formation, or zygote formation while abolishing the formation of ookinetes and further transmission of the parasites in mosquitoes...
February 27, 2024: MBio
https://read.qxmd.com/read/38409506/nucleosome-bound-nr5a2-structure-reveals-pioneer-factor-mechanism-by-dna-minor-groove-anchor-competition
#18
JOURNAL ARTICLE
Wataru Kobayashi, Anna H Sappler, Daniel Bollschweiler, Maximilian Kümmecke, Jérôme Basquin, Eda Nur Arslantas, Siwat Ruangroengkulrith, Renate Hornberger, Karl Duderstadt, Kikuë Tachibana
Gene expression during natural and induced reprogramming is controlled by pioneer transcription factors that initiate transcription from closed chromatin. Nr5a2 is a key pioneer factor that regulates zygotic genome activation in totipotent embryos, pluripotency in embryonic stem cells and metabolism in adult tissues, but the mechanism of its pioneer activity remains poorly understood. Here, we present a cryo-electron microscopy structure of human NR5A2 bound to a nucleosome. The structure shows that the conserved carboxy-terminal extension (CTE) loop of the NR5A2 DNA-binding domain competes with a DNA minor groove anchor of the nucleosome and releases entry-exit site DNA...
February 26, 2024: Nature Structural & Molecular Biology
https://read.qxmd.com/read/38386558/nr5a2-ensures-inner-cell-mass-formation-in-mouse-blastocyst
#19
JOURNAL ARTICLE
Yanhua Zhao, Meiting Zhang, Jiqiang Liu, Xinglin Hu, Yuchen Sun, Xingwei Huang, Jingyu Li, Lei Lei
Recent studies have elucidated Nr5a2's role in activating zygotic genes during early mouse embryonic development. Subsequent research, however, reveals that Nr5a2 is not critical for zygotic genome activation but is vital for the gene program between the 4- and 8-cell stages. A significant gap exists in experimental evidence regarding its function during the first lineage differentiation's pivotal period. In this study, we observed that approximately 20% of embryos developed to the blastocyst stage following Nr5a2 ablation...
February 21, 2024: Cell Reports
https://read.qxmd.com/read/38381606/systematic-evaluation-of-retroviral-ltrs-as-cis-regulatory-elements-in-mouse-embryos
#20
JOURNAL ARTICLE
Jian Yang, Lauryn Cook, Zhiyuan Chen
In mammals, many retrotransposons are de-repressed during zygotic genome activation (ZGA). However, their functions in early development remain elusive largely due to the challenge to simultaneously manipulate thousands of retrotransposon insertions in embryos. Here, we applied CRISPR interference (CRISPRi) to perturb the long terminal repeat (LTR) MT2_Mm, a well-known ZGA and totipotency marker that exists in ∼2,667 insertions throughout the mouse genome. CRISPRi robustly perturbed 2,485 (∼93%) MT2_Mm insertions and 1,090 (∼55%) insertions of the closely related MT2C_Mm in 2-cell embryos...
February 20, 2024: Cell Reports
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