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Osteosarcoma and huvos

Vincent Crenn, Kevin Biteau, Jérôme Amiaud, Clotilde Dumars, Romain Guiho, Luciano Vidal, Louis-Romée Le Nail, Dominique Heymann, Anne Moreau, François Gouin, Françoise Redini
Osteosarcoma, the most common malignant primary bone tumor, is currently treated with chemotherapy and surgery. The effectiveness of chemotherapy is evaluated by means of histological analysis of tumor necrosis, known as "the Huvos score". However, 25% of the patients initially considered good responders will relapse. In our practice, strong tissue heterogeneity around the residual viable cells of the osteosarcoma is observed, but this is not taken into account by the Huvos score, as it is only an average...
2017: American Journal of Cancer Research
L E A Delsin, G M Roberto, P F Fedatto, E E Engel, C A Scrideli, L G Tone, M S Brassesco
miRNAs have been identified as key regulators of almost all cellular processes, therefore, their dysregulation is involved with several diseases, including cancer. miRNAs specifically related to the metastastic cascade are called metastamiRs and can be involved with different steps of this process, including loss of adhesion. Osteosarcoma (OS) is the most common primary malignant pediatric bone tumor that often presents metastatic disease at diagnosis; therefore, a deeper study of adhesion-associated miRNAs could shed light on its pathophysiology...
September 25, 2017: Pathology Oncology Research: POR
Bin Liu, Zhi Zhang, E-Nuo Dai, Jia-Xin Tian, Jiang-Ze Xin, Liang Xu
Understanding the dynamic changes in connectivity of molecular pathways is important for determining disease prognosis. Thus, the current study used an inference of multiple differential modules (iMDM) algorithm to identify the connectivity changes of sub‑network to predict the progression of osteosarcoma (OS) based on the microarray data of OS at four Huvos grades. Initially, multiple differential co‑expression networks (M‑DCNs) were constructed, and weight values were assigned for each edge, followed by detection of seed genes in M‑DCNs according to the topological properties...
August 2017: Molecular Medicine Reports
Divya Vijayanarasimha, Sangeetha K Nayanar, Syam Vikram, Vijay M Patil, Satheesh Babu, Satheesan B
Although recent multimodality therapeutic protocols have led to improved survival in osteosarcoma (OS), the outcome still remains dismal. Ongoing international multicentric trials on OS aim to randomize patients for optimum management, based on histological response to NACT. The pathologic response to neoadjuvant chemotherapy (NACT) is the most important factor predicting prognosis. In this study of 23 cases of limb salvage surgery post neoadjuvant chemotherapy, mean age was 18.3 years, with male predominance...
June 2017: Indian Journal of Surgical Oncology
Seung Hyun Kim, Kyoo-Ho Shin, Seong-Hwan Moon, Youngho Kong, Jin-Suck Suh, Woo-Ick Yang
BACKGROUND AND OBJECTIVES: The purpose of this study was to establish a new concept for evaluating responses to neoadjuvant chemotherapy in osteosarcoma. METHODS: A total of 56 high-grade extremity osteosarcoma patients were retrospectively reviewed. A new conceptual method was derived from locations of residual viable tumor cells (LRVTC) after chemotherapy, whether extracompartmental or intracompartmental, rather than quantitative measurements of necrosis rates of tumor cells...
May 2017: Journal of Surgical Oncology
Daisuke Kubota, Nobuyoshi Kosaka, Tomohiro Fujiwara, Akihiko Yoshida, Yasuhito Arai, Zhiwei Qiao, Fumitaka Takeshita, Takahiro Ochiya, Akira Kawai, Tadashi Kondo
Osteosarcoma is the most common primary malignancy in bone. Patients who respond poorly to induction chemotherapy are at higher risk of adverse prognosis. The molecular basis for such poor prognosis remains unclear. We investigated miRNA expression in eight open biopsy samples to identify miRNAs predictive of response to induction chemotherapy and thus maybe used for risk stratification therapy. The samples were obtained from four patients with inferior necrosis (Huvos I/II) and four patients with superior necrosis (Huvos III/IV) following induction chemotherapy...
2016: Sarcoma
Achmad Fauzi Kamal, Heru Widyawarman, Kurniadi Husodo, Errol Untung Hutagalung, Wulyo Rajabto
AIM: to analyze the outcome and survival rate of osteosarcoma patients in our hospital as well as the factors affecting prognosis and functional outcome. METHODS: this is a retrospective cohort study of osteosarcoma patients in Cipto Mangunkusumo Hospital underwent limb salvage surgery (LSS), amputation, LSS + amputation, and refused surgery from year 1995 to 2014. The surgical decision was based on patient's age, staging, location, neurovascular involvement, Huvos type, functional demand, patient preference, and general condition...
July 2016: Acta Medica Indonesiana
M A Clara-Altamirano, D Y García-Ortega, J L Martínez-Tlahuel, H Martínez-Said, C H S Caro-Sánchez, G C García-Ruíz, C R Mejía-Salazar, M Cuellar-Hubbe
Osteosarcoma is the most frequent primary malignant bone tumor. It is characterized by osteoid production by tumor cells. Its most frequent location is in the metaphyses of long bones, but a purely diaphyseal presentation is reported in 10% of cases. We report the case of a female 25 year-old patient whose symptoms of pain and swelling of the right mid thigh started four months before, without an apparent cause. Femur X-rays showed a tumor in the femoral shaft. The MRI showed extension to soft tissues with no compromise of the neurovascular bundle...
January 2016: Acta Ortopédica Mexicana
Rosalvo Zósimo Bispo Júnior, Olavo Pires de Camargo
UNLABELLED: Would there be any difference in the prognosis for patients who presented, for example, 8% or 88% tumor necrosis induced by chemotherapy, even though both individuals were considered to be poor responders? The aim of this study was to compare the prognoses for different histological grades (Huvos grade I versus grade II), consequent to chemotherapy, among patients with primary osteosarcoma that was not metastatic at diagnosis. METHODS: Twenty-four patients admitted to a referral center between 2000 and 2004 were selected for the study...
July 2011: Revista Brasileira de Ortopedia
Seung Hyun Kim, Kyoo-Ho Shin, Ha Yan Kim, Yong Jin Cho, Jae Kyoung Noh, Jin-Suck Suh, Woo-Ick Yang
BACKGROUND: Metastasis is the most crucial prognostic factor in osteosarcoma. The goal of this study was to develop a new nomogram to predict the probability of metastasis in Enneking stage IIB extremity osteosarcoma after neoadjuvant chemotherapy and limb salvage surgery. METHODS: We examined medical records of 91 patients who had undergone surgery between March 1994 and March 2007. A nomogram was developed using multivariate logistic regression. The nomogram was validated internally by bootstrapping-method (200 repetitions) and externally in independent validation set (n = 34)...
2014: BMC Cancer
Sébastien Salas, Carine Jiguet-Jiglaire, Loic Campion, Catherine Bartoli, Frédéric Frassineti, Jean-Laurent Deville, André Maues De Paula, Fabien Forest, Pascal Jézéquel, Jean-Claude Gentet, Corinne Bouvier
BACKGROUND: The standard therapy regimen of conventional osteosarcoma includes neoadjuvant chemotherapy followed by surgical resection and postoperative chemotherapy. The percentage of necrotic tissue following induction chemotherapy is assessed by using the Huvos grading system, which classifies patients as "poor responders" (PR) and "good responders" (GR). The aim of this study was to identify molecular markers expressed differentially between good and poor responders to neoadjuvant chemotherapy in order to predict the response to chemotherapy in conventional osteosarcomas before beginning treatment...
2014: BMC Cancer
Daisuke Kubota, Kenta Mukaihara, Akihiko Yoshida, Hitoshi Tsuda, Akira Kawai, Tadashi Kondo
UNLABELLED: We attempted to identify biomarkers that would predict responsiveness of osteosarcoma (OS) to induction chemotherapy. Tumor tissues obtained by open biopsy before induction chemotherapy were investigated. On the basis of histological observations at the time of surgery and the Huvos grading system, 7 patients were classified as good responders and the other 6 as poor responders. Protein expression profiling was performed by two-dimensional difference gel electrophoresis. Among 3494 protein spots observed, the intensity of 33 spots was found to differ significantly between the two patient groups...
October 8, 2013: Journal of Proteomics
Anne Gomez-Brouchet, Corinne Bouvier, Anne-Valérie Decouvelaere, Frédérique Larousserie, Sébastien Aubert, Xavier Leroy, Jean-Marc Guinebretière, Aurore Coulomb, Elisabeth Cassagnau, Anne de Muret, Virginie Audard, Béatrice Marie, Gonzague de Pinieux
The survival of osteosarcoma and Ewing family tumours has been improved by the introduction of neoadjuvant chemotherapy. The response to preoperative chemotherapy is evaluated on the microscopic analysis of the surgical resection, by the percentage of tumour necrosis according to the Huvos and Rosen's grading. It remains the only reliable prognostic factor for patients and is used to guide the choice of post-operative chemotherapy. The macroscopic and microscopic management of the surgical resection (cf. supra) is essential and is the subject of a specific protocol...
December 2011: Annales de Pathologie
Kazutaka Kikuta, Naobumi Tochigi, Shigeru Saito, Tadakazu Shimoda, Hideo Morioka, Yoshiaki Toyama, Ako Hosono, Yoshiyuki Suehara, Yasuo Beppu, Akira Kawai, Setsuo Hirohashi, Tadashi Kondo
PURPOSE: We aimed to identify novel chemotherapy responsiveness biomarkers for osteosarcoma (OS) by investigating the global protein expression profile of 12 biopsy samples from OS patients. EXPERIMENTAL DESIGN: Six patients were classified as good responders and six as poor responders, according to the Huvos grading system. The protein expression profiles obtained by 2-D DIGE consisted of 2250 protein spots. RESULTS: Among them, we identified 55 protein spots whose intensity was significantly different (Bonferroni adjusted p-value<0...
May 2010: Proteomics. Clinical Applications
Saminathan S Nathan, Andrew G Huvos, Jorge E Casas-Ganem, Rui Yang, Irina Linkov, Rebecca Sowers, Gene R DiResta, Richard Gorlick, John H Healey
PURPOSE: We have previously shown that osteosarcomas have states of increased interstitial fluid pressure (IFP) which correlate with increased proliferation and chemosensitivity. In this study, we hypothesized that constitutively raised IFP in osteosarcomas regulates angiogenesis. MATERIALS AND METHODS: Sixteen patients with the clinical diagnosis of osteosarcomas underwent blood fl ow and IFP readings by the wick-in-needle method at the time and location of open biopsy...
December 2009: Annals of the Academy of Medicine, Singapore
Jyoti Bajpai, Meharchand Sharma, Vishnubhatla Sreenivas, Rakesh Kumar, Shivanand Gamnagatti, Shah Alam Khan, Shishir Rastogi, Arun Malhotra, Sameer Bakhshi
BACKGROUND: The vascular endothelial growth factor (VEGF) pathway is the key regulator of angiogenesis. In osteosarcoma baseline VEGF is of proven prognostic value but prognostic potential of post-NACT VEGF expression is largely unexplored. PROCEDURE: Treatment naive patients with osteosarcoma were subjected to initial staging workup followed by three cycles of neoadjuvant chemotherapy (NACT) and surgery; resected tumors were assessed for histological necrosis by Huvos grading...
December 2009: Pediatric Blood & Cancer
T Ueda, J H Healey, A G Huvos, M Ladanyi
Purpose. In a previous series of 25 human osteosarcoma samples studied for MYC gene amplification, we found amplification in two cases (8%), including one arising in association with Paget's disease (pagetic osteosarcoma). Based on this observation, we further investigated the prevalence of MYC gene amplification in pagetic osteosarcomas.Methods. MYC gene amplification was assessed by Southern blot analysis using frozen tissue samples in five cases of pagetic osteosarcoma and 53 cases of primary (non-pagetic) osteosarcoma...
1997: Sarcoma
Saminathan S Nathan, Andrew G Huvos, Jorge E Casas-Ganem, Rui Yang, Irina Linkov, Rebecca Sowers, Gene R DiResta, Richard Gorlick, John H Healey
We have previously shown that osteosarcomas (OS) have states of increased interstitial fluid pressure (IFP), which correlate with increased proliferation and chemosensitivity. In this study, we hypothesized that constitutively raised IFP in OS regulates angiogenesis. Sixteen patients with the clinical diagnosis of OS underwent blood flow and IFP readings by the wick-in-needle method at the time and location of open biopsy. Vascularity was determined by capillary density in the biopsy specimens. We performed digital image analysis of immunohistochemical staining for CD31, VEGF-A, VEGF-C, and TPA on paraffin-embedded tissue blocks of the biopsy samples...
November 2008: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
David R Lucas, Malti P Kshirsagar, J Sybil Biermann, Merlin R Hamre, Dafydd G Thomas, Scott M Schuetze, Laurence H Baker
Histologic response to chemotherapy is generally regarded as an independent prognostic variable in bone sarcomas, both osteosarcoma and Ewing's sarcoma. In soft tissue sarcomas, however, descriptions of histologic alterations from chemotherapy and correlative outcome studies are much more limited. Herein we report clinicopathological findings from a homogeneously treated group of 31 patients with tumor stage T2 grade 3 extremity soft tissue sarcomas treated with the same neoadjuvant chemotherapy followed by surgical excision, treated by the same medical oncologist and orthopedic surgeon...
April 2008: Oncologist
Tadahiko Kubo, Sajida Piperdi, Jeremy Rosenblum, Cristina R Antonescu, Wen Chen, Han-Soo Kim, Andrew G Huvos, Rebecca Sowers, Paul A Meyers, John H Healey, Richard Gorlick
The purpose of this review was to determine whether imatinib mesylate (STI571, Gleevec) has a role in the treatment of osteosarcoma. The expression of platelet-derived growth factor (PDGF) receptor and its ligand was examined in a panel of surgical specimens obtained from 54 osteosarcoma patients, and then the expression was compared with prognosis. The effects of imatinib mesylate on growth and molecular events in 10 patient-derived osteosarcoma cell cultures were investigated. Immunohistochemical studies demonstrated frequent expression of PDGF-AA (80...
May 15, 2008: Cancer
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