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Soilless Culture

Thi Dao Vu, Cyril Jousse, Nathalie Pawlicki-Jullian, Séverine Schiltz, Thi Kieu Oanh Nguyen, Thi Le Minh Tran, Laure-Anne Bouquet, Alain Hehn, Michèle Boitel-Conti, Julie Moussaron, Flore Biteau, Corinne Assaf-Ducrocq, Christophe Robin, Frédéric Bourgaud, Armand Guckert, Eric Gontier
BACKGROUND: The production of secondary metabolites through the culture of entire plants is of great interest. Soilless culture, such as hydroponics, enables the control of plant growth and metabolism. Specific environmental conditions must be developed to maximize the productivity of medicinal plants used as efficient natural bioreactors. METHODS: The nutrient solution of newly established hydroponic cultures ofDatura innoxia Mill. were inoculated with Agrobacterium rhizogenes (A...
December 2018: Plant Science: An International Journal of Experimental Plant Biology
Sheng Ma, Zhen Chen, Feng Juan Yang, Xiu Feng Wang, Min Wei, Qing Hua Shi, Yan Li, Biao Gong
To get optimal nitrogen (N), phosphorus (P2 O5 ) and potassium (K2 O) concentrations range for high quality and yield of eggplant with vermiculite as cultivation substrate, three factors and quadratic saturation D-optimal regression (310) were used to establish a ternary quadratic mathematical model with N, P2 O5 and K2 O concentrations as independent variables and eggplant yield and quality as objective functions. The results showed that yield and quality of eggplants were significantly influenced by N, P2 O5 and K2 O concentrations...
September 2018: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
Yan-Jiao Yin, Chuan-Jiao Chen, Shi-Wei Guo, Ke-Ming Li, Yu-Nan Ma, Wu-Mei Sun, Fu-Rong Xu, Yong-Xian Cheng, Xian Dong
The root of Panax notoginseng ( P. notoginseng ) is one of the most highly valuable medicinal herbs in China owing to its pronounced hemostatic and restorative properties. Despite this important fact, growing P. notoginseng is seriously limited by root-rot diseases. In studies aimed at developing a solution to this problem, environment-friendly essential oils (EOs) of five medicinal plants of the family Zingiberaceae were tested for their inhibitory effects on the growth of three main soil pathogens associated with the root-rot diseases of P...
2018: Frontiers in Plant Science
Md Asaduzzaman, Md Raihan Talukder, Hideyuki Tanaka, Makoto Ueno, Mikiko Kawaguchi, Shozo Yano, Takuya Ban, Toshiki Asao
Chronic kidney disease patients are restricted to foods with high potassium content but our daily diets including melon are rich in potassium. Therefore, we investigated the production of low-potassium melon through hydroponic nutrient management in soilless culture using perlite substrate during autumn season of 2012, 2014 and spring season of 2016. In the first study, melon plants were supplied with 50% standard 'Enshi' nutrient solution until first 2 weeks of culture. In 3rd and 4th week, amount of applied potassium was 50, 75, 100, and 125% of required potassium nitrate for each plant per week (based on our previous study)...
2018: Frontiers in Plant Science
Kenji Takemura, Ryosuke Endo, Yoshiaki Kitaya
The goal of this study was to establish a system for co-culturing microalgae and crop plants with biogas digestate. We examined growth performances of E. gracilis and L. sativa co-cultured using a commercial liquid fertilizer designed for soilless culture supplemented with vitamins and ammonium. This solution simulated the filtrate of nitrified biogas digestate derived from the organic fraction of municipal solid waste but was supplemented with insufficient plant nutrients (Mg, Fe and Mn). The specific growth rate of the co-cultured E...
August 28, 2018: Environmental Technology
Taewon Moon, Tae In Ahn, Jung Eek Son
In existing closed-loop soilless cultures, nutrient solutions are controlled by the electrical conductivity (EC) of the solution. However, the EC of nutrient solutions is affected by both growth environments and crop growth, so it is hard to predict the EC of nutrient solution. The objective of this study was to predict the EC of root-zone nutrient solutions in closed-loop soilless cultures using recurrent neural network (RNN). In a test greenhouse with sweet peppers ( Capsicum annuum L.), data were measured every 10 s from October 15 to December 31, 2014...
2018: Frontiers in Plant Science
Giuseppe Cristiano, Gjok Vuksani, Vincenzo Tufarelli, Barbara De Lucia
The most common substrate for potted ornamental plants is prepared with Sphagnum peat; however, the cost and declining availability of high-quality peat, due to environmental constraints, make it necessary to investigate for alternative organic materials. The present study aimed to determine the effects of partial compost replacement with peat and the optimum electrical conductivity (EC) level of the nutrient solution in potted weeping lantana [ L. montevidensis (Spreng.) Briq.] under a recirculating soilless system...
March 22, 2018: Plants (Basel, Switzerland)
David Renault, Franck Déniel, Jessica Vallance, Emilie Bruez, Jean-Jacques Godon, Patrice Rey
In soilless culture, slow filtration is used to eliminate plant pathogenic microorganisms from nutrient solutions. The present study focused on the characterization and the potential functions of microbial communities colonizing the nutrient solutions recycled on slow filters during a whole cultivation season of 7 months in a tomato growing system. Bacterial microflora colonizing the solutions before and after they flew through the columns were studied. Two filters were amended with Pseudomonas putida (P-filter) or Bacillus cereus strains (B-filter), and a third filter was a control (C-filter)...
July 2018: Microbial Ecology
Xiao Yang, Lei Feng, Li Zhao, Xiaosong Liu, Danial Hassani, Danfeng Huang
BACKGROUND: Lettuce is a significant source of antioxidants and bioactive compounds. Nitrate is a cardinal fertilizer in horticulture and influences vegetable yield and quality; however, the negative effects of nitrate on the biosynthesis of flavonoids require further study. It is expected that using fertilizers containing organic nitrogen (N) could promote the synthesis of health-promoting compounds. RESULTS: Lettuces were hydroponically cultured in media containing 9 mmol L-1 nitrate or 9 mmol L-1 glycine for 4 weeks...
January 2018: Journal of the Science of Food and Agriculture
Kyle Benzle, Katrina Cornish
BACKGROUND: Plant roots are used as an efficient target tissue for plant transformation assays. In root propagable species transformed roots are able to regenerate into whole plants without the addition of exogenous hormones, thus avoiding somaclonal variation associated with many plant transformation protocols. Plants grown in soil or soilless solid medium have roots that tend to be extremely delicate and are difficult to sterilize in advance of plant transformation experiments. Axenic tissue culture plants grown on semi-solid media are slow to produce large amounts of biomass compared to plants grown in solution-based media...
2017: Plant Methods
Hao Zhang, Minmin Yang, Qian Luan, Hu Tang, Fenghong Huang, Xia Xiang, Chen Yang, Yuping Bao
Cellulose anionic hydrogels were successfully prepared by dissolving TEMPO-oxidized cellulose nanofibers in NaOH/urea aqueous solution and being cross-linked with epichlorohydrin. The hydrogels exhibited microporous structure and high hydrophilicity, which contribute to the excellent water absorption property. The growth indexes, including the germination rate, root length, shoot length, fresh weight, and dry weight of the seedlings, were investigated. The results showed that cellulose anionic hydrogels with suitable carboxylate contents as plant growth regulators could be beneficial for seed germination and growth...
May 17, 2017: Journal of Agricultural and Food Chemistry
Aiju Liu, JinXin Li, Menghong Li, Xiao Yin Niu, Jun Wang
Metals are always found in the environment as mixtures rather than as solitary elements. Only a limited number of studies have developed appropriate models that incorporate bioavailability to estimate the toxicity of heavy-metal mixtures. In the present study, we explored the applicability of two extended biotic ligand model (BLM) approaches-BLM-f mix and BLM-toxicity unit (TU)-to predict and interpret mixture toxicity with the assumption that interactions between metal ions obey the BLM theory. Exposure assays of single and mixed metals were performed with inoculums of an ammonia-oxidizing bacterium SD5 isolated from soil...
February 2017: Archives of Environmental Contamination and Toxicology
Li Liu, Mei-Ling Ji, Min Chen, Ming-Yue Sun, Xi-Ling Fu, Ling Li, Dong-Sheng Gao, Cui-Ying Zhu
Strawberry fruits (cv. Benihoppe, Tochiotome, Sachinoka, and Guimeiren) were harvested and evaluated the flavor and nutritional parameters. By principal component analysis and hierarchical clustering analysis, differences were observed based on the volatile compounds composition, sugar and acid concentration, sweetness, and total soluble sugars/total organic acids of the four varieties. A total of 37, 48, 65, and 74 volatile compounds were identified and determined in cv. Benihoppe, Tochiotome, Sachinoka, and Guimeiren strawberry fruits extracted by head-space solid-phase microextraction (HS-SPME), respectively...
November 2016: Food Science & Nutrition
Kenji Takemura, Ryosuke Endo, Toshio Shibuya, Yoshiaki Kitaya
We investigated the possibility of using Euglena gracilis to convert digestate from methane fermentation of organic wastes into a medium for soilless crop culture. The growth of E. gracilis cultured with aqueous solutions containing filtrate of raw digestate at 1-30% (v/v) and nitrified digestate at 10-100% (v/v) was examined. Concentrations of plant macronutrient ions in nitrified digestate before and after culturing E. gracilis were also examined. Specific growth rates in aqueous solutions containing filtrate of raw digestate at 1-10% and nitrified digestate at 10-100% showed no significant differences, respectively (0...
September 2017: Environmental Technology
Daniela Borgognone, Youssef Rouphael, Mariateresa Cardarelli, Luigi Lucini, Giuseppe Colla
Leaf extracts of cultivated cardoon (Cynara cardunculus L. var. altilis DC) are an important source of phenols. Soilless culture represents an important and alternative tool to traditional agriculture, since it allows a precise control of plant nutrition and the maximization of yield and quality of the product. Reducing N supply, while keeping quantity as high as possible is desirable for environmental and health-related reasons, especially that N deficiency can lead to improved concentrations of secondary plant metabolites...
2016: Frontiers in Plant Science
Ji-Sun Kim, Chul Geon An, Jong-Suk Park, Yong Pyo Lim, Suna Kim
In this study, we investigated carotenoid profiles and contents from 27 types of paprika with different colors (red, orange, and yellow), shapes (blocky and conical), and cultivation methods (soil and soilless). We simultaneously analyzed 12 kinds of carotenoids using UPLC equipped with an HSS T3 column for 30 min, and we identified six kinds of carotenoids in red paprika and nine types in orange and yellow paprika. Zeaxanthin concentrations in orange paprika were in the range of 85.06±23.37-151.39±5.94 mg/100 g dry weight (dw), which shows that orange paprika is a great source of zeaxanthin...
June 15, 2016: Food Chemistry
Oliver Grunert, Emma Hernandez-Sanabria, Ramiro Vilchez-Vargas, Ruy Jauregui, Dietmar H Pieper, Maaike Perneel, Marie-Christine Van Labeke, Dirk Reheul, Nico Boon
The choice of soilless growing medium for plant nutrition, growth and support is crucial for improving the eco-sustainability of the production in horticultural systems. As our current understanding of the functional microbial communities inhabiting this ecosystem is still limited, we examined the microbial community development of the two most important growing media (organic and mineral) used in open soilless horticultural systems. We aimed to identify factors that influence community composition over time, and to compare the distribution of individual taxa across growing media, and their potential functionality...
January 5, 2016: Scientific Reports
O Grunert, E Hernandez-Sanabria, M Perneel, M C Van Labeke, D Reheul, N Boon
Despite numerous preventative measures, the hairy roots syndrome is an increasing problem in greenhouse horticulture. A recent survey of 177 tomato, cucumber and eggplant growers in Flanders (Belgium) revealed an increase of this disease in the last two years, with about 26% of all the tomato crops showing the syndrome. In this study, we compared the physicochemical and microbial community characteristics of inorganic and organic growing media in relation to the presence of the causative agent of the hairy roots, the plant pathogen Agrobacterium rhizogenes...
2014: Communications in Agricultural and Applied Biological Sciences
Tanya R Copley, Konstantinos A Aliferis, Suha Jabaji
Many studies have investigated the effect of biochar on plant yield, nutrient uptake, and soil microbial populations; however, little work has been done on its effect on soilborne plant diseases. To determine the effect of maple bark biochar on Rhizoctonia damping-off, 11 plant species were grown in a soilless potting substrate amended with different concentrations of biochar and inoculated or not with Rhizoctonia solani anastomosis group 4. Additionally, the effect of biochar amendment on R. solani growth and metabolism in vitro was evaluated...
October 2015: Phytopathology
Stefanie Delbeke, Siele Ceuppens, Claudia Titze Hessel, Irene Castro, Liesbeth Jacxsens, Lieven De Zutter, Mieke Uyttendaele
Strawberries are an important fruit in Belgium in both production and consumption, but little information is available about the presence of Salmonella and Shiga toxin-producing Escherichia coli (STEC) in these berries, the risk factors in agricultural production, and possible specific mitigation options. In 2012, a survey was undertaken of three soil and three soilless cultivation systems in Belgium. No Salmonella spp. were isolated. No STEC was detected in the strawberry samples (0 of 72), but STEC was detected by PCR in 11 of 78 irrigation water and 2 of 24 substrate samples...
April 2015: Applied and Environmental Microbiology
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