Yu Cai, Lu Han, Desheng Zhu, Jing Peng, Jianping Li, Jie Ding, Jiaying Luo, Ronghua Hong, Kan Wang, Wenbin Wan, Chong Xie, Xiajun Zhou, Ying Zhang, Yong Hao, Yangtai Guan
Cell-based assays (CBAs) and radioimmunoprecipitation assay (RIPA) are the most sensitive methods for identifying anti-acetylcholine receptor (AChR) antibody in myasthenia gravis (MG). But CBAs are limited in clinical practice by transient transfection. We established a stable cell line (KL525) expressing clustered AChR by infecting HEK 293T cells with dual lentiviral vectors expressing the genes encoding the human AChR α1, β1, δ, ϵ and the clustering protein rapsyn. We verified the stable expression of human clustered AChR by immunofluorescence, immunoblotting, and real-time PCR...
2021: Frontiers in Immunology