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Implant sonication

Robert M Hoffman, Takashi Murakami, Kei Kawaguchi, Kentaro Igarashi, Yuying Tan, Shukuan Li, Qinghong Han
Methionine (MET) is a general target in cancer due to the excess requirement of MET by cancer cells. MET has been effectively restricted by recombinant methioninase (rMETase) in mouse models of cell-line tumors. This chapter reviews the efficacy of rMETase on patient-derived orthotopic xenograft (PDOX) mouse models of human cancer. Ewing's sarcoma is a recalcitrant disease even though development of multimodal therapy has improved patients' outcome. A Ewing's sarcoma was implanted in the right chest wall of nude mice to establish a PDOX model...
2019: Methods in Molecular Biology
Richard D Swenson, Jamie L Kraemer, Henry D Clarke, Thomas E Grys, Adam J Schwartz
BACKGROUND: With advancements in manufacturing technology, custom orthopedic implants have become commercially available. A new concern with these implants is what to do when custom heat-sensitive components are contaminated. While intraoperative decontamination protocols for dropped autograft tissue have been described, no literature describes an intraoperative protocol for decontaminating one-of-a-kind polyethylene implants. The purpose of this work is to describe and evaluate polyethylene decontamination protocols using materials found in the average operating suite that could be used intraoperatively...
January 30, 2019: Knee
Justus Bürger, Doruk Akgün, Patrick Strube, Michael Putzier, Matthias Pumberger
PURPOSE: In total joint replacement, culturing of the sonication fluid of removed implants has proven to be more sensitive than conventional periprosthetic tissue culture for the microbiological diagnosis of prosthetic joint infection. However, its role in postoperative spinal implant infection (PSII) is not well investigated. Therefore, the aim of this study was to determine the validity of sonication in detecting infection following instrumented spine surgery. METHODS: In this prospective controlled consecutive cohort study, patients undergoing spinal revision between September 2016 and March 2018 were analyzed...
January 17, 2019: European Spine Journal
C E Tanase, M Golozar, S M Best, R A Brooks
Plasma electrolytic oxidation (PEO) has been demonstrated to be an effective surface treatment for enhancing the osteoconduction and osseointegration of commercially pure α-Ti (CP α-Ti) dental implant materials for clinical application. To explore the feasibility of extending the application of PEO to low-modulus β-type titanium alloys for load-bearing orthopaedic implants, a thorough understanding of the effect of substrate material on the biological performance of the PEO-treated surface is required. A 10 kW 50 Hz KeroniteTM processing unit was used to modify the surface of low-modulus near β-Ti13Nb13Zr and β-Ti45Nb substrates...
December 26, 2018: Colloids and Surfaces. B, Biointerfaces
Sambri Andrea, Maso Alessandra, Storni Elisa, Donati Maria Elena, Pederzoli Annalisa, Dallari Dante, Bianchi Giuseppe, Donati Davide
BACKGROUND: In a two-stage exchange protocol for prosthetic joint infection (PJI), bacteria surviving over the antibiotic-loaded cement spacers may cause the persistence of infection with renewed clinical symptoms following the surgery. Culture after sonication of removed prosthesis is more sensitive than conventional periprosthetic tissue culture for the microbiological diagnosis of PJI. The aim of this study was to assess whether sonication of the spacer at the time of the second-stage procedure may improve the diagnosis of persistent PJI...
December 12, 2018: Journal of Microbiological Methods
Eric Gomez-Urena, Rafael J Sierra, Kerryl E Greenwood-Quiantance, Melissa J Karau, James M Steckelberg, Robin Patel
Diagnosis of persistent infection at the time of reimplantation for staged revision of infected arthroplasties is challenging. Implant sonication culture for the diagnosis of prosthetic joint infection (PJI) has improved sensitivity compared to standard periprosthetic tissue culture. We report our experience with periprosthetic tissue culture and sonication culture of antimicrobial agent-containing cement spacers (ACSs) collected during second stages of staged revisions for arthroplasty infection. We studied 87 ACSs from 66 patients undergoing two-stage revision arthroplasty for PJI submitted for sonication culture along with conventional periprosthetic tissue cultures...
December 12, 2018: Journal of Clinical Microbiology
Ludwig Knabl, Bettina Kuppelwieser, Astrid Mayr, Wilfried Posch, Michaela Lackner, Débora Coraҫa-Huber, Adrian Danita, Michael Blauth, Cornelia Lass-Flörl, Dorothea Orth-Höller
Stabilization of fractures with internal fixation devices is a common procedure and implant-associated infections are a dreaded complication. The exact pathomechanism is not completely understood; however, microbial colonization of osteosynthesis material is considered a trigger for infection. This study aimed to determine the colonization rate of osteosynthesis implants in patients with no clinical or laboratory signs of infection, using two methods, conventional culture and polymerase chain reaction (PCR) of sonication fluid...
December 3, 2018: MicrobiologyOpen
Hyung-Jin Park, Hee-June Kim, Shukho Kim, Seong-Min Kim, Jong-Uk Mun, Jungmin Kim, Hee-Soo Kyung
Background: The purpose of this study was to evaluate the usefulness of sonication technique for microbiological diagnosis and the sterility of the recycled autoclaved femoral components from infected total knee arthroplasty (TKA) using a sonication method. Methods: Nineteen femoral implants explanted from patients with infected TKA were sterilized with a standard autoclave method. Standard culture of the fluid before and after sonication of the sterilized implants was performed to detect pathogenic microorganisms...
December 2018: Clinics in Orthopedic Surgery
Iman Roohaniesfahani, Jie Wang, Young Jung No, Christian de Candia, Xinchao Miao, Zufu Lu, Jeffrey Shi, David L Kaplan, Xinquan Jiang, Hala Zreiqat
Injectable silk hydrogels are ideal carriers of therapeutic agents due to their biocompatibility and low immunogenicity. Injectable silk hydrogels for bone regeneration have been previously developed but often utilize expensive biologics. In this study, we have developed an injectable silk composite incorporated with a triphasic ceramic called MSM-10 (54 Mg2 SiO4 , 36 Si3 Sr5 and 10 MgO (wt%)) capable of simultaneously releasing magnesium, silicon, and strontium ions into its environment. These ions have been previously reported to possess therapeutic effects for bone regeneration...
January 1, 2019: Materials Science & Engineering. C, Materials for Biological Applications
Magdalena Pilz, Kevin Staats, Selma Tobudic, Ojan Assadian, Elisabeth Presterl, Reinhard Windhager, Johannes Holinka
BACKGROUND: One of the most commonly identified pathogens responsible for orthopaedic implant infection is Staphylococcus epidermidis, which can form biofilms on surfaces. Currently, orthopaedic implants made of various surface materials are available, each with features influencing osseointegration, biocompatibility, and adherence of bacteria to the surface, which is the first step in biofilm formation. The aim of this experimental study was to investigate the effect of a high tribologic-resistant 2...
November 8, 2018: Clinical Orthopaedics and related Research
Thorsten Wichmann, T Fintan Moriarty, Iris Keller, Stefan Pfister, Vanessa Deggim-Messmer, Emanuel Gautier, Fabian Kalberer, Peter P Koch, Peter Wahl
INTRODUCTION: Knitted cotton outer gloves offer protection against surgical glove perforation and provide improved grip on instruments. These gloves absorb blood and other fluids during surgery, and may therefore also accumulate contaminating bacteria. To date, there is no published data on microbial contamination of such gloves during surgery. METHODS: Knitted cotton outer gloves used in primary and revision hip and knee arthroplasty from two Swiss hospitals were analysed by quantitative bacteriology...
November 7, 2018: Archives of Orthopaedic and Trauma Surgery
Stephanie M C Smith, Brandon M Bianski, Brent A Orr, Gretchen Harknett, Arzu Onar-Thomas, Richard J Gilbertson, Thomas E Merchant, Martine F Roussel, Christopher L Tinkle
PURPOSE: Craniospinal irradiation (CSI) is a crucial component of treatment for medulloblastoma (MB), a brain tumor clinically stratified into prognostically distinct molecular subgroups. Preclinical models of clinically relevant CSI offer the potential to study radiation dose and volume effects in these subgroups and to identify subgroup-specific combination adjuvant therapies, particularly for very-high-risk MB in which treatments are often unsuccessful. METHODS AND MATERIALS: The commercially available Small Animal Radiation Research Platform equipped with a motorized variable collimator was used for image-guided CSI...
March 1, 2019: International Journal of Radiation Oncology, Biology, Physics
Shawn Vasoo
With the increasing number of prosthetic joints replaced annually worldwide, orthopedic implant-associated infections (OIAI) present a considerable burden. Accurate diagnostics are required to optimize surgical and antimicrobial therapy. Sonication fluid cultures have been shown in multiple studies to improve the microbiological yield of OIAIs, but uptake of sonication has not been widespread in many routine clinical microbiology laboratories. In this issue, M. Dudareva and colleagues (J Clin Microbiol 56:e00688-18, 2018, https://doi...
December 2018: Journal of Clinical Microbiology
Wonhye Lee, Phillip Croce, Ryan W Margolin, Amanda Cammalleri, Kyungho Yoon, Seung-Schik Yoo
BACKGROUND: Low-intensity transcranial focused ultrasound (tFUS) has emerged as a new non-invasive modality of brain stimulation with the potential for high spatial selectivity and penetration depth. Anesthesia is typically applied in animal-based tFUS brain stimulation models; however, the type and depth of anesthesia are known to introduce variability in responsiveness to the stimulation. Therefore, the ability to conduct sonication experiments on awake small animals, such as rats, is warranted to avoid confounding effects of anesthesia...
September 19, 2018: BMC Neuroscience
Peilin Chen, Zhigang Wu, Alex Leung, Xiaobo Chen, Euphemie Landao-Bassonga, Junjie Gao, Lianzhi Chen, Monica Zheng, Felix Yao, Hong Yang, Lars Lidgren, Brent Allan, Yinong Liu, Tao Wang, Minghao Zheng
Alveolar bone loss is a common problem that affects dental implant placement. A barrier between the bone substitute and gingiva that can prevent fibro-tissue ingrowth, bacterial infection and induce bone formation is a key factor in improving the success of alveolar ridge reconstruction. This study aims to develop a bioactive collagen barrier material for guided bone regeneration, that is coupled with anti-bacterial and anti-inflammatory properties. We have evaluated two silver coating methods and found controllable and precise coating achieved by sonication compared with sputtering...
October 2, 2018: Biomedical Materials
Hang-Kang Chen, Shu-Mei Zhang, Junn-Liang Chang, Hsin-Chien Chen, Yi-Chun Lin, Cheng-Ping Shih, Huey-Kang Sytwu, Mei-Cho Fang, Yuan-Yung Lin, Chao-Yin Kuo, Ai-Ho Liao, Yueng-Hsiang Chu, Chih-Hung Wang
The use of cisplatin (CDDP), the most common chemotherapy drug for head and neck cancer, is limited by its undesirable side effects, especially nephrotoxicity. We investigated ultrasound microbubbles (USMB) as a tool to increase the local intra-tumoral CDDP level while decreasing systemic CDDP cytotoxicity. We allowed CDDP to interact with human serum albumin and then sonicated the resulting CDDP‒albumin complex to generate CDDP-loaded MBs (CDDP-MBs). We then established a head-and-neck tumor-bearing mouse model by implanting FaDu-fLuc/GFP cells into severe combined immunodeficiency mice and used IVIS® bioluminescence imaging to determine the tumor xenograft formation and size...
September 5, 2018: Cancers
Zhang-Lai Li, Yun-Fei Hou, Bao-Qing Zhang, Yi-Fan Chen, Qi Wang, Kai Wang, Zhao-Yu Chen, Xiao-Wei Li, Jian-Hao Lin
OBJECTIVE: The aim of this study is to identify the common microorganisms causing PJI as well as the drug-resistant spectrum for each microorganism, to help orthopaedic surgeons to choose appropriate antibiotics. METHOD: One hundred and sixty patients who suffered from failure of primary or revision total hip or knee arthroplasty for different reasons were prospectively recruited. These patients underwent revision or re-revision total hip or knee arthroplasty in our institution between August 2013 to August 2016...
August 2018: Orthopaedic Surgery
Haiou Yang, Qijie Zhu, Hongfei Qi, Xianhu Liu, Meixia Ma, Qiang Chen
Additive manufacturing enabled the fabrication of porous titanium (PT) with customized porosity and mechanical properties. However, functionalization of PT surfaces with bioactive coatings is being challenged due to sophisticated geometry and highly porous structure. In this study, a facile flow-casting technique was developed to produce homogeneous 45S5 bioactive glass (BG) coatings on the entire surface of PT. The coating weight as a function of BG concentration in a BG-PVA slurry was investigated to achieve controllable coating yield without blocking macropore structure...
August 27, 2018: Materials
Vincent Prinz, Simon Bayerl, Nora Renz, Andrej Trampuz, Peter Vajkoczy, Tobias Finger
BACKGROUND: Antimicrobial treatment of ventriculoperitoneal (VP) shunt infections is challenging when the causative pathogen is unknown. OBJECTIVE: To evaluate the value of sonication of explanted shunt-devices to improve the microbiological detection rate. METHODS: All consecutive patients undergoing revision surgery due to suspected VP-shunt infection from January 2015 to February 2017 were evaluated. Intraoperative tissue samples, wound swabs, and cerebrospinal fluid (CSF) were collected for microbiological examination...
August 17, 2018: Neurosurgery
Nora Renz, Stasa Mudrovcic, Carsten Perka, Andrej Trampuz
BACKGROUND: The definition criteria and clinical characteristics of implant-associated infection (IAI) caused by Cutibacterium (formerly Propionibacterium) spp. are poorly known. We analyzed microbiologically proven Cutibacterium orthopedic IAI in a prospective cohort. METHODS: Patients with periprosthetic joint infections (PJI) and fixation device-associated infections (FDAI) caused by Cutibacterium spp. were prospectively included. IAI was defined by significant growth of Cutibacterium spp...
2018: PloS One
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