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Hyaluronic Acid Bone Regeneration

Yajie Zhang, Hong Chen, Tingting Zhang, Yue Zan, Tianyu Ni, Yi Cao, Jine Wang, Min Liu, Renjun Pei
Bone-marrow-derived mesenchymal stem cells possess great potential for tissue engineering and regenerative medicine. In the work, an injectable BMSCs-laden hydrogel system was formed by enzyme-catalyzed crosslinking of hyaluronic acid-tyramine and chondroitin sulfate-tyramine in the presence of hydrogen peroxide and horseradish peroxidase, which was used as a 3D scaffold to explore the behavior of the mesenchymal stem cells. Afterward, the gelation rate, mechanical properties, as well as the degradation process of the scaffold were well characterized and optimized...
March 2019: Materials Science & Engineering. C, Materials for Biological Applications
Mohammed Abbas, Mohammed Alkaff, Asim Jilani, Haneen Alsehli, Laila Damiati, Mamdooh Kotb, Moahmmed Abdelwahed, Fahad Alghamdi, Gauthaman Kalamegam
BACKGROUND: Mesenchymal stem cells (MSCs) and/or biological scaffolds have been used to regenerate articular cartilage with variable success. In the present study we evaluated cartilage regeneration using a combination of bone marrow (BM)-MSCs, HyalofastTM and/or native cartilage tissue following full thickness surgical cartilage defect in rabbits. METHODS: Full-thickness surgical ablation of the medial-tibial cartilage was performed in New Zealand white (NZW) rabbits...
October 2018: Tissue Engineering and Regenerative Medicine
Kunyu Zhang, Zhaofeng Jia, Boguang Yang, Qian Feng, Xiao Xu, Weihao Yuan, Xingfu Li, Xiaoyu Chen, Li Duan, Daping Wang, Liming Bian
The targeted and simultaneous delivery of diverse cargoes with vastly different properties by the same vehicle is highly appealing but challenging. Here, a bioactive nanocomposite hydrogel based on hyaluronic acid and self-assembled pamidronate-magnesium nanoparticles for the localized elution and on-demand simultaneous release of bioactive ions and small molecule drugs is described. The obtained nanocomposite hydrogels exhibit excellent injectability and efficient stress relaxation, thereby allowing easy injection and consequent adaptation of hydrogels to bone defects with irregular shapes...
December 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
M Yazan, I D Kocyigit, F Atil, U Tekin, Z B Gonen, M E Onder
The mechanism of osseointegration is related to many factors, including the quality of the bone, the biocompatibility and surface characteristics of the implant material, the surgical technique, and functional loading. The purpose of this study was to investigate the effects of hyaluronic acid gel on the osseointegration of implants placed in defined areas of the mandible in rabbits. Hyaluronic acid is known to have an osteoinductive effect during regeneration of bony defects, and we thought that it might also have a favourable effect on osseointegration, a specialised mechanism to heal bone...
December 14, 2018: British Journal of Oral & Maxillofacial Surgery
Benjamin Mesure, Patrick Menu, Jagadeesh Kumar Venkatesan, Magali Cucchiarini, Émilie Velot
Musculoskeletal pathologies, especially those affecting bones and joints, remain a challenge for regenerative medicine. The main difficulties affecting bone tissue engineering are the size of the defects, the need for blood vessels and the synthesis of appropriate matrix elements in the engineered tissue. Indeed, the cartilage is an avascular tissue and consequently has limited regenerative abilities. Thanks to their self-renewal, plasticity and immunomodulatory properties, mesenchymal stem cells (MSCs) became a central player in tissue engineering, and have already been shown to be able to differentiate towards chondrogenic or osteogenic phenotypes...
December 5, 2018: Current Stem Cell Research & Therapy
Tingting Zhang, Hong Chen, Yajie Zhang, Yue Zan, Tianyu Ni, Min Liu, Renjun Pei
Many patients suffer from bone injury and self-regeneration is not effective. Developing new strategies for effective bone injury repair is highly desired. Herein, collagen, an important component of the extracellular matrix, was modified with glycidyl methacrylate. The water solubility and photochemical cross-linking ability of the resulting collagen derivative was then improved. Thereafter, BMSC-laden hydrogel was fabricated using collagen modified with glycidyl methacrylate and hyaluronic acid modified with methacrylic anhydride under UV light in the presence of I 2959...
November 22, 2018: Colloids and Surfaces. B, Biointerfaces
Hassan Rammal, Camille Bour, Marie Dubus, Laura Entz, Léa Aubert, Sophie C Gangloff, Sandra Audonnet, Nicolae B Bercu, Fouzia Boulmedais, Cedric Mauprivez, Halima Kerdjoudj
The use of inorganic calcium/phosphate supplemented with biopolymers has drawn lots of attention in bone regenerative medicine. While inflammation is required for bone healing, its exacerbation alters tissue regeneration/implants integration. Inspired by bone composition, a friendly automated spray-assisted system was used to build bioactive and osteoinductive calcium phosphate/chitosan/hyaluronic acid substrate (CaP-CHI-HA). Exposing monocytes to CaP-CHI-HA resulted in a secretion of pro-healing VEGF and TGF-β growth factors, TNF-α, MCP-1, IL-6 and IL-8 pro-inflammatory mediators but also IL-10 anti-inflammatory cytokine along with an inflammatory index below 1...
November 3, 2018: International Journal of Molecular Sciences
Jaewoo Pak, Jung Hun Lee, Natalie Jieun Pak, Kwang Seung Park, Jeong Ho Jeon, Byeong Chul Jeong, Sang Hee Lee
Osteoarthritis (OA) is one of the most common debilitating disorders. Recently, numerous attempts have been made to improve the functions of the knees by using different forms of mesenchymal stem cells (MSCs). In Korea, bone marrow concentrates and cord blood-derived stem cells have been approved by the Korean Food and Drug Administration (KFDA) for cartilage regeneration. In addition, an adipose tissue-derived stromal vascular fraction (SVF) has been allowed by the KFDA for joint injections in human patients...
September 29, 2018: Journal of Visualized Experiments: JoVE
Li-Jun Deng, Yan-Lin Wu, Xian-Hua He, Ke-Nan Xie, Lu Xie, Yi Deng
The strategy of a functional biomimetic system built to obtain the positive cellular response remains a field of topical interest. In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy. Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently...
October 11, 2018: Journal of Biomaterials Science. Polymer Edition
Jeanne Adiwinata Pawitan
Traditional Chinese and Korean medicine uses various manipulations on acupuncture points/acupoints that are located along imaginary lines on the surface of a human body, which are called 'meridians'. Acupuncture has been used from the ancient time till now to cure various diseases, including for the purpose of regenerative medicine. In various studies, meridians are alternatively called as Bong-Han ducts, primo vessels, or hyaluronic-acid rich ducts, while acupoints are called Bong-Han corpuscles, primo nodes, or hyaluronic-acid rich nodes...
October 2018: Journal of Traditional and Complementary Medicine
Masoud Hasany, Ashish Thakur, Nayere Taebnia, Firoz Babu Kadumudi, Mohammad-Ali Shahbazi, Malgorzata Karolina Pierchala, Soumyaranjan Mohanty, Gorka Orive, Thomas L Andresen, Casper Bindzus Foldager, Soheila Yaghmaei, Ayyoob Arpanaei, Akhilesh K Gaharwar, Mehdi Mehrali, Alireza Dolatshahi-Pirouz
Despite the promise of hydrogel-based stem cell therapies in orthopedics, a significant need still exists for the development of injectable microenvironments capable of utilizing the  regenerative potential of donor cells. Indeed, the quest for biomaterials that can direct stem cells into bone without the need of external factors has been the "Holy Grail" in orthopedic stem cell therapy for decades. To address this challenge, we have utilized a combinatorial approach to screen over 63 nanoengineered hydrogels made from alginate, hyaluronic acid, and two-dimensional nanoclays...
October 5, 2018: ACS Applied Materials & Interfaces
P T Sudheesh Kumar, Saeed Hashimi, Siamak Saifzadeh, Saso Ivanovski, Cédryck Vaquette
Vertical bone augmentation of the jaws is required when the height of bone is insufficient at the site of dental implant placement. In this proof of concept study, we investigated the potential of a biphasic polycaprolactone construct combined with a hyaluronic acid based hydrogel loaded with recombinant human bone morphogenetic growth factor-2 (BMP-2) for vertical bone regeneration. The biphasic scaffold consisted of an outer shell manufactured by fused deposition modelling, mimicking native cortical bone and providing mechanical and space maintenance properties essential for bone formation...
November 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Gokila S, Gomathi T, Vijayalakshmi K, Alsharani Faleh A, Anil Sukumaran, Sudha P N
Bone tissue engineering put emphasis on fabrication three-dimensional biodegradable porous scaffolds that supporting bone regeneration and functional bone tissue formation. In the present work, we prepared novel 3D tripolymeric scaffolds of nanochitosan (NCS)/silk fibroin (SF)/hyaluronic acid (HA) ternary blends and demonstrating the synergistic effect of scaffolds and its use in tissue engineering applications. The physico-chemical characterization of the prepared scaffold was evaluated by FTIR, XRD and SEM studies...
December 2018: International Journal of Biological Macromolecules
Han Tsung Liao, Ming-Jin Tsai, Manuri Brahmayya, Jyh-Ping Chen
For bone regeneration, a biocompatible thermo-gelling hydrogel, hyaluronic acid- g -chitosan- g -poly(N-isopropylacrylamide) (HA-CPN) was used as a three-dimensional organic gel matrix for entrapping rabbit adipose-derived stem cells (rASCs). Biphasic calcium phosphate (BCP) ceramic microparticles were embedded within the gel matrix as a mineralized bone matrix, which was further fortified with platelet-rich plasma (PRP) with osteo-inductive properties. In vitro culture of rASCs in HA-CPN and HA-CPN/PRP/BCP was compared for cell proliferation and osteogenic differentiation...
August 27, 2018: International Journal of Molecular Sciences
Zhe Cai, Yu Feng, Chentian Li, Kedi Yang, Tianhao Sun, Lei Xu, Yan Chen, Chun-Hoi Yan, William Weijia Lu, Kwong-Yuen Chiu
OBJECTIVE: To investigate efficacy of Chinese medicine magnoflorine combined with hyaluronic acid (HA)-gel in promoting subchondral bone (SCB) regeneration and attenuating cartilage degeneration in early osteoarthritis (OA). METHODS: MC3T3-E1 under magnoflorine treatment was assayed by XTT to determine cell viability. Cell proliferation was reflected through cell cycle. Osteoblast mineralization was stained by Alizarin Red. Standardized bone canal of 1 mm in diameter and 4 mm in depth was made on tibial medial plateau of 4-month-old Dunkin-Hartley spontaneous knee OA guinea pigs...
November 2018: Bone
Omer B Agrali, Selin Yildirim, Hafize O Ozener, Kemal N Köse, Dilek Ozbeyli, Merva Soluk-Tekkesin, Leyla Kuru
Hyaluronic acid (HA) constitutes one of the major components of the extracellular matrix domain in almost all mammals. The aim of this study was to evaluate the regenerative capacity of HA matrix in rat calvarial bone defects and compare with those of different combinations of resorbable collagen membrane (M) and bovine-derived xenograft (G). Twenty-four 3-month-old male Sprague-Dawley rats weighing 200-250 g were included. Control group was created by leaving one defect empty from 2 critical size defects with 5 mm diameter formed in the calvarial bones of 8 rats...
2018: BioMed Research International
Shinya Sasaki, Katsuhiro Takeda, Manabu Takewaki, Kazuhisa Ouhara, Mikihito Kajiya, Noriyoshi Mizuno, Tsuyoshi Fujita, Hidemi Kurihara
BACKGROUND: Recently, brain-derived neurotrophic factor (BDNF)/high molecular weight hyaluronic acid (HMW-HA) complex with flap surgery has been shown to promote periodontal tissue regeneration. The objective of this study was to evaluate the effects of local subgingival application of BDNF/HMW-HA complex adjunctive to scaling and root planning (SRP) on ligature-induced periodontitis in dogs. MATERIALS AND METHODS: The dogs were divided into four treatment groups: no treatment (control), SRP alone, SRP followed by local application of HMW-HA (SRP+HMW-HA), and SRP followed by local application of BDNF (500 μg/ml)/ HMW-HA complex (SRP+BDNF/HMW-HA)...
July 20, 2018: Journal of Periodontology
Lang Li, Xin Duan, Zhaoxin Fan, Long Chen, Fei Xing, Zhao Xu, Qiang Chen, Zhou Xiang
Mesenchymal stem cells (MSCs) and hyaluronic acid (HA) have been found in previous studies to have great potential for medical use. This study aimed to investigate the therapeutic effects of bone marrow mesenchymal stem cells (BMSCs) combined with HA on articular cartilage repair in canines. Twenty-four healthy canines (48 knee-joints), male or female with weight ranging from 5 to 6 kg, were operated on to induce cartilage defect model and divided into 3 groups randomly which received different treatments: BMSCs plus HA (BMSCs-HA), HA alone, and saline...
July 2, 2018: Scientific Reports
Dai-Xu Wei, Jin-Wei Dao, Guo-Qiang Chen
To avoid large open surgery using scaffold transplants, small-sized cell carriers are employed to repair complexly shaped tissue defects. However, most cell carriers show poor cell adherences and viability. Therefore, polyhydroxyalkanoate (PHA), a natural biopolymer, is used to prepare highly open porous microspheres (OPMs) of 300-360 µm in diameter, combining the advantages of microspheres and scaffolds to serve as injectable carriers harboring proliferating stem cells. In addition to the convenient injection to a defected tissue, and in contrast to poor performances of OPMs made of polylactides (PLA OPMs) and traditional less porous hollow microspheres (PHA HMs), PHA OPMs present suitable surface pores of 10-60 µm and interconnected passages with an average size of 8...
August 2018: Advanced Materials
Adriana Gilarska, Joanna Lewandowska-Łańcucka, Wojciech Horak, Maria Nowakowska
Studies on synthesis, physico-chemical and biological properties of novel biomimetic materials, potentially useful as injectable hydrogels are presented. These materials are in situ prepared chemically crosslinked collagen/chitosan/hyaluronic acid-based hydrogels exhibiting potential for tissue regeneration. Optimization of hydrogels involved testing the effect of various concentration of crosslinking agent (genipin) as well as different ratios of biopolymers used on their properties. The changes in the content of hyaluronic acid and in the genipin concentration used have been shown to be crucial...
October 1, 2018: Colloids and Surfaces. B, Biointerfaces
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