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"Blood" AND (Culture OR Cultures)

Iara Barreto Neves Oliveira, Rodrigo Saar Gomes, Larissa Fonseca Gomides, Jéssica Cristina Dos Santos, Marcos Alexandre Diniz Carneiro, Fátima Ribeiro-Dias, Denise Sisterolli Diniz
OBJECTIVE: Multiple sclerosis (MS) is a multifactorial chronic disease that affects the central nervous system (CNS). Toll-like receptors (TLRs) play a central role in cytokine production after pathogen- and danger-associated molecular patterns (PAMPs and DAMPs) and contribute to CNS damage in MS patients. Here, we evaluated the effects of interferon (IFN)-β treatment in TLR2 and TLR4-dependent cytokine production and mRNA expression in whole-blood cell cultures from MS patients. METHODS: We evaluated cytokine production by ELISA from whole-blood cell culture supernatants and mRNA expression by real-time polymerase chain reaction in peripheral blood mononuclear cells (PBMCs)...
March 21, 2019: Neuroimmunomodulation
Sudipta Patra, Chaitanya Tellapragada, Vandana Kalwaje Eshwara, Chiranjay Mukhopadhyay
AIMS: In this present study, the utility of a newly developed Loop mediated isothermal amplification (LAMP) and real-time PCR assays designed to amplify the virB gene region of B. melitensis were evaluated from human clinical specimens. METHODS AND RESULTS: 54 culture confirmed cases of brucellosis and 54 culture negative but clinically suspected cases of brucellosis were included in the study. Whole blood, serum and other non-blood specimens were collected and subjected for blood culture using automatic blood culture system, serological tests, LAMP assay and real time PCR...
March 21, 2019: Journal of Applied Microbiology
R S Nannan Panday, S Wang, P M van de Ven, T A M Hekker, N Alam, P W B Nanayakkara
BACKGROUND: Blood cultures remain the gold standard for detecting bacteremia despite their limitations. The current practice of blood culture collection is still inefficient with low yields. Limited focus has been given to the association between timing of specimen collection at different time points during admission and their yield. METHODS: We carried out a retrospective observational study by analyzing all 3,890 sets of cultures collected from the 1,962 admitted patients over the seven-month period of this study...
2019: PloS One
Francisco Domper Bardají, Aurora Gil Rendo, Soledad Illescas Fernández-Bermejo, Roberto Patón Arenas, Alicia Hernández Albújar, Francisco Martín Dávila, Cristina Murillo Lázaro, Mónica Sánchez Alonso, Maite Serrano Dueñas, Ana Sobrino López, Fernando Ramírez Esteso, Jesús Martín Fernández
BACKGROUND: small intestinal bacterial overgrowth (SIBO) and bacterial translocation across the intestinal wall have been allegedly associated with non-alcoholic fatty liver (NAFL). Our goal was to study such alleged association in morbidly obese patients. PATIENTS AND METHODS: patients with morbid obesity were consecutively included prior to bariatric surgery. Exclusion criteria included normal liver biopsy, other causes of liver disease, and duodenal mucosal atrophy...
March 21, 2019: Revista Española de Enfermedades Digestivas
S A Bright, A J Byrne, E Vandenberghe, P V Browne, A M Mcelligott, M J Meegan, D C Williams
The nitrostyrene scaffold was previously identified as a lead target structure for the development of effective compounds targeting Burkitt's lymphoma. The present study aimed to develop these compounds further in haematological malignancies, including chronic lymphocytic leukaemia (CLL). Cellular viability, flow cytometry and lactate dehydrogenase assays, amongst others, were used to examine the effects of nitrostyrene compounds on CLL cells, including a cell line representing disease with poor prognosis (HG‑3) and in ex vivo CLL patient samples (n=14)...
March 15, 2019: Oncology Reports
Yan-Ling Qiu, Xiao Chen, Ya-Li Hou, Yan-Juan Hou, Song-Bo Tian, Yu-He Chen, Li Yu, Min-Hai Nie, Xu-Qian Liu
The aim of the present study was to compare the characteristics of acellular dermal matrix (ADM), small intestinal submucosa (SIS) and Bio‑Gide scaffolds with acellular vascular matrix (ACVM)‑0.25% human‑like collagen I (HLC‑I) scaffold in tissue engineering blood vessels. The ACVM‑0.25% HLC‑I scaffold was prepared and compared with ADM, SIS and Bio‑Gide scaffolds via hematoxylin and eosin (H&E) staining, Masson staining and scanning electron microscope (SEM) observations. Primary human gingival fibroblasts (HGFs) were cultured and identified...
March 21, 2019: Molecular Medicine Reports
Xiaoxia Li, Xiangyu Ding, Peng Shi, Yiqing Zhu, Yidie Huang, Qin Li, Jinmiao Lu, Zhiping Li, Lin Zhu
Neonatal sepsis (NS) remains a major cause of morbidity and mortality in neonates, but data on the etiology and antibiotic susceptibility patterns of pathogens are limited. The aim of this study was to analyze the clinical characteristics, risk factors, and the antibiotic susceptibility patterns of pathogenic microbes associated with NS at a tertiary children's hospital in Shanghai, China.Episodes of blood culture-proven sepsis in the neonatal intensive care unit (NICU) of Children's Hospital of Fudan University from January 2013 to August 2017 were retrospectively reviewed...
March 2019: Medicine (Baltimore)
Tahereh Derakhshan, Rudra Bhowmick, James H Meinkoth, Jerry Ritchey, Heather Gappa-Fahlenkamp
Mast cells (MCs) play critical roles in the pathogenesis of IgE- and non-IgE-mediated immune responses, as well as host defense against parasites, bacteria, and viruses. Due to the effect of the extracellular matrix components on the tissue morphogenesis and cell behavior, utilizing a tissue model that mimics MC microenvironmental conditions <i>in vivo</i> has greater relevance for <i>in vitro</i> studies. For this work, MCs were developed within a connective tissue-equivalent model and cell function was examined in response to an allergen...
March 21, 2019: Tissue Engineering. Part A
Sarah Berndt, Antoine Turzi, Brigitte Pittet-Cuénod, Ali Modarressi
Nowadays autologous fibroblast application for skin repair presents an important clinical interest. In most cases, in vitro skin cell culture is mandatory. However, cell expansion using xenogeneic or allogenic culture media presents some disadvantages, such as the risk of infection transmission or slow cell expansion. In this study, we investigated an autologous culture system to expand human skin fibroblast cells in vitro with the patient's own platelet-rich-plasma (PRP). Human dermal fibroblasts were isolated from patients undergoing abdominoplasty and blood was collected to prepare non-activated PRP using the CuteCellTM PRP medical device...
March 21, 2019: Tissue Engineering. Part A
Grit Lutze, Anna Haarmann, Jules A Demanou Toukam, Kerstin Buttler, Jörg Wilting, Jürgen Becker
Development of lymphatics takes place during embryogenesis, wound healing, inflammation, and cancer. We previously showed that Wnt5a is an essential regulator of lymphatic development in the dermis of mice, however, the mechanisms of action remained unclear. Here, whole-mount immunostaining shows that embryonic day (ED) 18.5 Wnt5a-null mice possess non-functional, cyst-like and often blood-filled lymphatics, in contrast to slender, interconnected lymphatic networks of Wnt5a+/- and wild-type (wt) mice. We then compared lymphatic endothelial cell (LEC) proliferation during ED 12...
March 18, 2019: Scientific Reports
Beatriz Baragaña, Barbara Forte, Ryan Choi, Stephen Nakazawa Hewitt, Juan A Bueren-Calabuig, João Pedro Pisco, Caroline Peet, David M Dranow, David A Robinson, Chimed Jansen, Neil R Norcross, Sumiti Vinayak, Mark Anderson, Carrie F Brooks, Caitlin A Cooper, Sebastian Damerow, Michael Delves, Karen Dowers, James Duffy, Thomas E Edwards, Irene Hallyburton, Benjamin G Horst, Matthew A Hulverson, Liam Ferguson, María Belén Jiménez-Díaz, Rajiv S Jumani, Donald D Lorimer, Melissa S Love, Steven Maher, Holly Matthews, Case W McNamara, Peter Miller, Sandra O'Neill, Kayode K Ojo, Maria Osuna-Cabello, Erika Pinto, John Post, Jennifer Riley, Matthias Rottmann, Laura M Sanz, Paul Scullion, Arvind Sharma, Sharon M Shepherd, Yoko Shishikura, Frederick R C Simeons, Erin E Stebbins, Laste Stojanovski, Ursula Straschil, Fabio K Tamaki, Jevgenia Tamjar, Leah S Torrie, Amélie Vantaux, Benoît Witkowski, Sergio Wittlin, Manickam Yogavel, Fabio Zuccotto, Iñigo Angulo-Barturen, Robert Sinden, Jake Baum, Francisco-Javier Gamo, Pascal Mäser, Dennis E Kyle, Elizabeth A Winzeler, Peter J Myler, Paul G Wyatt, David Floyd, David Matthews, Amit Sharma, Boris Striepen, Christopher D Huston, David W Gray, Alan H Fairlamb, Andrei V Pisliakov, Chris Walpole, Kevin D Read, Wesley C Van Voorhis, Ian H Gilbert
Malaria and cryptosporidiosis, caused by apicomplexan parasites, remain major drivers of global child mortality. New drugs for the treatment of malaria and cryptosporidiosis, in particular, are of high priority; however, there are few chemically validated targets. The natural product cladosporin is active against blood- and liver-stage Plasmodium falciparum and Cryptosporidium parvum in cell-culture studies. Target deconvolution in P. falciparum has shown that cladosporin inhibits lysyl-tRNA synthetase ( Pf KRS1)...
March 20, 2019: Proceedings of the National Academy of Sciences of the United States of America
Zerelda Esquer Garrigos, Merit P George, Prakhar Vijayvargiya, Eugene M Tan, Saira Farid, Omar M Abu Saleh, Paul A Friedman, James M Steckelberg, Daniel C DeSimone, Walter R Wilson, Larry M Baddour, M Rizwan Sohail
OBJECTIVE: To describe and compare the clinical presentation, management, and outcomes of cardiovascular implantable electronic device (CIED) infections due to gram-negative bacteria (GNB) and CIED infections due to gram-positive bacteria (GPB). PATIENTS AND METHODS: We retrospectively reviewed all CIED infection cases at Mayo Clinic from January 1, 1992, through December 31, 2015. Cases were classified based on positive microbiology data from extracted devices or blood cultures...
March 17, 2019: Mayo Clinic Proceedings
I A Rakityanskaya, T S Ryabova, A A Kalashnikova
BACKGROUND: Patients with chronic herpes virus infection develop impaired IFN-α and IFN-γ products due to secondary immunodeficiency, which leads to impaired elimination of the intracellular virus and the development of chronic recurrent infection. It has been shown that IFN-γ is a potent immunoregulatory cytokine and has an antiviral effect. OBJECTIVES: The aim of the study is to study the effect of Ingaron therapy on the dynamics of IFN-α and IFN-γ production and the clinical picture in patients with chronic Epstein-Barr virus infection (ChEBVI)...
2019: Voprosy Virusologii
Mahmoud Ali, Sandeep Kowkuntla, Derick J Delloro, Csaba Galambos, Deep Hathi, Siegfried Janz, Monica Shokeen, Chakrapani Tripathi, Hongwei Xu, Jisung Yuk, Fenghuang Zhan, Michael H Tomasson, Melissa L Bates
Obesity is the only known modifiable risk factor for multiple myeloma (MM), an incurable cancer of bone marrow plasma cells. The mechanism linking the two is unknown. Obesity is associated with an increased risk of sleep apnea, which results in chronic intermittent hypoxia (CIH), and drives solid tumor aggressiveness. Given the link between CIH and solid tumor progression, we tested the hypothesis that CIH drives the proliferation of MM cells in culture and their engraftment and progression in vivo. Malignant mouse 5TGM1 cells were cultured in CIH, static hypoxia (SH) or normoxia as a control in custom, gas permeable plates...
March 20, 2019: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Eriko Makino, Nobuhisa Nakamura, Megumi Miyabe, Mizuho Ito, Saki Kanada, Masaki Hata, Tomokazu Saiki, Kazunori Sango, Hideki Kamiya, Jiro Nakamura, Ken Miyazawa, Shigemi Goto, Tatsuaki Matsubara, Keiko Naruse
AIMS/INTRODUCTION: Dental pulp stem cells (DPSCs) can be easily obtained from teeth for general orthodontic reasons. We have previously reported the therapeutic effects of DPSC transplantation for diabetic polyneuropathy. Since abundant secretomes from DPSCs are considered to play a central role in the improvement of diabetic polyneuropathy, we investigated whether direct injection of DPSC-conditioned media (DPSC-CM) into hindlimb skeletal muscles ameliorates diabetic polyneuropathy in diabetic rats...
March 20, 2019: Journal of Diabetes Investigation
Bethânia Ferreira Bastos, Flavya Mendes de Almeida, Beatriz Brener
Tritrichomonas foetus is a parasite that has been definitively identified as an agent of trichomonosis, a disease characterized by chronic diarrhea. T. foetus colonizes portions of the feline large intestine, and manifests as chronic and recurrent diarrhea with mucus and fresh blood, which is often unresponsive to common drugs. Diagnosis of a trichomonad infection is made by either the demonstration of the trophozoite on a direct fecal smear, fecal culture and subsequent microscopic examination of the parasite, or extraction of DNA in feces and amplification by the use of molecular tools...
March 11, 2019: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
Andresa Guimarães, Juliana Macedo Raimundo, Raisa Braul Rodrigues, Maristela Peckle Peixoto, Huarrisson Azevedo Santos, Marcos Rogério André, Rosangela Zacarias Machado, Cristiane Divan Baldani
Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA)...
March 11, 2019: Brazilian Journal of Veterinary Parasitology
Beverly Gor, Vishnu P Nepal, Rashmi Dongardive, V K Dorai, Mala Pande
Objective: The South Asian Health Needs Assessment was conducted to collect health status information on the rapidly growing Asian Indian (AI) community in the Houston area. Many were highly educated and reported high income levels, factors usually associated with better health outcomes. This study examined the relationship between socioeconomic advantage and the health behaviors and health outcomes of AIs. Methods: We analyzed cross-sectional survey data from a convenience sample of 1416 AIs...
January 2019: Health Services Research and Managerial Epidemiology
Yurong Yang, Hui Dong, Ruijing Su, Tongyi Li, Nan Jiang, Chunlei Su, Longxian Zhang
Toxoplasma gondii has been found to infect almost all warm-blooded animals; however, some hosts lack direct evidence of T. gondii infection. The red panda ( Ailurus fulgens ) is an endangered species that mainly lives in temperate forests of South Asia. Here, T. gondii infection in red pandas from zoos in China were reported. Antibodies to T. gondii were found in 14.3% (2/14) of red pandas via the modified agglutination test (MAT) with a cut-off titer of 1:25. One viable T. gondii strain was isolated from tissues of red panda and designated as TgRedpandaCHn1...
April 2019: International Journal for Parasitology. Parasites and Wildlife
Ethan Burns, Kartik Anand, Gonzalo Acosta, Malcolm Irani, Betty Chung, Abhishek Maiti, Ibrahim Ibrahim, Lawrence Rice
Autosplenectomy (AS) is a known complication of diseases such as sickle cell anemia, celiac disease, and inflammatory bowel disease. We report the first known case of AS due to paroxysmal nocturnal hemoglobinuria (PNH). A 24-year-old Caucasian male had evidence of hemolytic anemia at the age of 14 and was diagnosed with PNH at the age of 16. He had recurrent episodes of sepsis due to dialysis line infections from poor hygiene, and blood cultures had been positive for multiple organisms including Staphylococcus aureus , Enterococcus faecalis , and Streptococcus pneumoniae ...
2019: Case Reports in Hematology
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