María Alejandra Puentes-Tellez, Oscar F Sánchez, Felipe Rojas-Rodriguez, Eliana Benincore-Flóre, Hector Barbosa, Carlos J Alméciga Díaz
Mucopolysaccharidosis type IVA (MPS IVA) is a lysosomal storage disease produced by the deficiency of the N-acetylgalactosamine-6-sulfate sulfatase (GALNS) enzyme, leading to glycosaminoglycans (GAGs) accumulation. Since currently available treatments remain limited and unspecific, novel therapeutic approaches are essential for the disease treatment. In an attempt to reduce treatment limitations, gene therapy rises as a more effective and specific alternative. We present in this study the delivery assessment of GALNS and sulfatase-modifying factor 1 (SUMF1) genes via HIV-1 derived lentiviral vectors into fibroblasts from MPS IVA patients...
February 22, 2021: Gene
Selena Alonso, Marta Vidal, Gemma Ruiz-Olalla, Raquel González, Chenjerai Jairoce, M Nelia Manaca, Miquel Vázquez-Santiago, Reyes Balcells, Anifa Vala, María Ruperez, Pau Cisteró, Laura Fuente-Soro, Evelina Angov, Ross L Coppel, Benoit Gamain, David Cavanagh, James G Beeson, Arsenio Nhacolo, Esperança Sevene, John J Aponte, Eusébio Macete, Ruth Aguilar, Alfredo Mayor, Clara Menéndez, Carlota Dobaño, Gemma Moncunill
OBJECTIVES: Maternal Plasmodium falciparum-specific antibodies may contribute to protect infants against severe malaria. Our main objective was to evaluate the impact of maternal HIV infection and placental malaria on the cord blood levels and efficiency of placental transfer of IgG and IgG subclasses. METHODS: In a cohort of 341 delivering HIV- and HIV+ mother from southern Mozambique, we measured total IgG and IgG subclasses in maternal and cord blood pairs by quantitative suspension array technology against eight P...
February 23, 2021: Journal of Infection
Xinqi Xie, Junling Ma, P van den Driessche
The activation and proliferation of naive CD4 T cells produce helper T cells, and increase the susceptible population in the presence of HIV. This may cause backward bifurcation. To verify this, we construct a simple within-host HIV model that includes the key variables, namely healthy naive CD4 T cells, helper T cells, infected CD4 T cells and virus. When the viral basic reproduction number R0 is less than unity, we show theoretically and numerically that bistability for RC <R0 <1 can be caused by a backward bifurcation due to a new susceptible population produced by activation of healthy naive CD4 T cells that become helper T cells...
February 23, 2021: Mathematical Biosciences
Jane Tucker, Joanne Salas, Zidong Zhang, Richard Grucza, Jeffrey F Scherrer
The CDC Guideline for Prescribing Opioids for Chronic Pain cautioned against high dose prescribing but did not provide guidance on type of opioid for new pain episodes. We determined if new prescriptions for Schedule II opioids vs. tramadol decreased in the 18 months after vs. before the CDC guideline and if this decrease was associated with physician specialty. New opioid prescriptions, provider type and covariates were measured using a nationally distributed, Optum® de-identified Electronic Health Record (EHR) data base...
February 23, 2021: Preventive Medicine
Chelsy Chesterman, Eddy Arnold
This work presents a method for introducing synthetic symmetry into protein crystallization samples using an antibody fragment termed a diabody (Dab). These Dabs contain two target binding sites, and engineered disulfide bonds have been included to modulate Dab flexibility. The impacts of Dab engineering have been observed through assessment of thermal stability, small-angle X-ray scattering, and high-resolution crystal structures. Complexes between the engineered Dabs and HIV-1 reverse transcriptase (RT) bound to a high-affinity DNA aptamer were also generated to explore the capacity of engineered Dabs to enable the crystallization of bound target proteins...
February 23, 2021: Structure
María Rocío Hernández-Morales, Eleazar Mancilla-Hernández
BACKGROUND: Trimethoprim with sulfamethoxazole (TMP-SMX) is the drug of choice for the prophylaxis of AIDS-associated comorbidities. OBJECTIVE: To compare the efficacy and safety of two schemes of desensitization to TMP-SMX in HIV-positive patients. METHODS: A study was conducted from March 2018 to October 2019; it included HIV-positive patients who presented an adverse skin reaction to TMP-SMX; fifteen of them received desensitization scheme 1, which lasted ten days, and five patients received scheme 2, which lasted six hours...
July 2020: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
Stuart R Jefferys, Samuel D Burgos, Jackson J Peterson, Sara R Selitsky, Anne-Marie Turner, Lindsey I James, Yi-Hsuan Tsai, Alisha R Coffey, David M Margolis, Joel Parker, Edward P Browne
Transcriptional silencing of HIV in CD4 T cells generates a reservoir of latently infected cells that can reseed infection after interruption of therapy. As such, these cells represent the principal barrier to curing HIV infection, but little is known about their characteristics. To further our understanding of the molecular mechanisms of latency, we characterized a primary cell model of HIV latency in which infected cells adopt heterogeneous transcriptional fates. In this model, we observed that latency is a stable, heritable state that is transmitted through cell division...
February 26, 2021: PLoS Pathogens
Mohib Ullah Kakar, Muhammad Matloob, Rongji Dai, Yulin Deng, Kifayat Ullah, Ihsan Ullah Kakar, Ghulam Khaliq, Muhammad Umer, Zhoaib Ahmed Bhutto, Sarfarz Ali Fazlani, Muhammad Zubair Mehboob
DC-SIGN receptor articulated by macrophages and dendritic cells is encoded by CD209 gene and plays a role to activate and proliferate the T-lymphocytes in response of virus attack. The dysfunctional activity of DC-SIGN receptor because of missense SNPs can lead to cause dengue haemorrhage fever, HIV-1 infection etc. Out of 11 transcripts of CD209, all missense SNPs of canonical transcript were retrieved from Ensembl database and evaluated by their deleteriousness by using Polyphen-2, PMut, SIFT, MutPred, PROVEAN and PhD-SNP together with stimulation of its complete 3D structure...
2021: PloS One
James W Bruce, Megan Bracken, Edward Evans, Nathan Sherer, Paul Ahlquist
Previously, we reported that cellular transcription factor ZASC1 facilitates DNA-dependent/RNA-independent recruitment of HIV-1 TAT and the cellular elongation factor P-TEFb to the HIV-1 promoter and is a critical factor in regulating HIV-1 transcriptional elongation (PLoS Path e1003712). Here we report that cellular transcription factor ZBTB2 is a novel repressor of HIV-1 gene expression. ZBTB2 strongly co-immunoprecipitated with ZASC1 and was dramatically relocalized by ZASC1 from the cytoplasm to the nucleus...
February 26, 2021: PLoS Pathogens
Taylor Fleming, Alexandra B Collins, Geoff Bardwell, Al Fowler, Jade Boyd, M J Milloy, Will Small, Ryan McNeil
Antiretroviral therapy (ART) dispensing is strongly associated with treatment adherence. Among illicit drug-using populations, whom experience greater structural barriers to adherence, directly administered antiretroviral therapy (DAAT) is often regarded as a stronger predictor of optimal adherence over self-administered medications. In Vancouver, Canada, people living with HIV (PLHIV) who use drugs and live in low-income housing are a critical population for treatment support. This group is typically able to access two key DAAT models, daily delivery and daily pickup, in addition to ART self-administration...
2021: PloS One
(no author information available yet)
No abstract text is available yet for this article.
March 2021: Journal of the Association of Nurses in AIDS Care: JANAC
Yijia Li, Behzad Etemad, Ruth Dele-Oni, Radwa Sharaf, Charlie C Gao, Xu Yu, Jonathan Z Li
BACKGROUND: HIV proviral sequencing overcomes the limit of plasma viral load requirement by detecting all the "archived mutations", but the clinical relevance remains to be evaluated. METHODS: We included 25 participants with available proviral sequences (both intact and defective sequences available) and utilized the genotypic sensitivity score (GSS) to evaluate the level of resistance in their provirus and plasma virus. Defective sequences were further categorized as sequences with and without hypermutations...
February 22, 2021: AIDS
Amanda K Jan, Julia V Moore, Richard J Wang, Maggie Mcging, Carly K Farr, Daniela Moisi, Marlena Hartman-Filson, Robert Kerruish, Diane Jeon, Eula Lewis, Kristina Crothers, Michael M Lederman, Peter W Hunt, Laurence Huang
OBJECTIVES: Studies have shown that persons with HIV (PWH) may be at increased risk for chronic lung diseases and lung function abnormalities, which may be associated with immune activation. We tested the association of a panel of twelve immune activation and inflammation biomarkers with spirometry and single-breath diffusing capacity for carbon monoxide (DLco). DESIGN: Cross-sectional, observational study. METHODS: Participants were enrolled from the I AM OLD cohort of PWH at two US sites...
February 22, 2021: AIDS
Davide De Francesco, Caroline A Sabin, Alan Winston, Patrick W G Mallon, Jane Anderson, Marta Boffito, Nicki D Doyle, Lewis Haddow, Frank A Post, Jaime H Vera, Memory Sachikonye, Susan Redline, Ken M Kunisaki
OBJECTIVES: To evaluate the agreement between self-reported sleep measures and insomnia with objectively-measured sleep parameters in people with HIV (PWH) and HIV-negative individuals. DESIGN: Cross-sectional analysis of PWH and lifestyle-similar HIV-negative individuals. METHODS: Self-reported measures included time spent in bed, sleep onset latency and a validated insomnia questionnaire. Objective measures were assessed via 7-days/nights of actigraphy data to determine average and intra-individual variability of several sleep measures (including time spent in bed and onset latency)...
February 23, 2021: AIDS
Suzanne M Mccluskey, Kimia Kamelian, Nicholas Musinguzi, Simone Kigozi, Yap Boum, Mwebesa B Bwana, Conrad Muzoora, Zabrina L Brumme, Mary Carrington, Jonathan Carlson, Brian Foley, Peter W Hunt, Jeffrey N Martin, David R Bangsberg, P Richard Harrigan, Mark J Siedner, Jessica E Haberer, Guinevere Q Lee
OBJECTIVE: Dolutegravir (DTG) is now a preferred component of first-line antiretroviral therapy (ART). However, prevalence data on natural resistance to integrase inhibitors (INSTIs) in circulating non-subtype B HIV-1 in sub-Saharan Africa is scarce. Our objective is to report prevalence of pre-treatment integrase polymorphisms associated with resistance to INSTIs in an ART-naïve cohort with diverse HIV-1 subtypes. DESIGN: We retrospectively examined HIV-1 integrase sequences from Uganda...
February 23, 2021: AIDS
Giovanni Guaraldi, Stefano Calza, Jovana Milic, Andrea Calcagno, Emanuele Focà, Matteo Rota, Stefano Renzetti, Anna Celotti, Matteo Siano, Benedetto Maurizio Celesia, Stefania Piconi, Giuseppe Vittorio de Socio, Anna Maria Cattelan, Giancarlo Orofino, Agostino Riva, Silvia Nozza, Giovanni di Perri
OBJECTIVE: The aim of this study was to explore weight gain (WG) in people living with HIV (PLWH) ≥65 years who switch to a DTG based regimen (DTG-s) vs remaining INSTI-naive (INSTI-n) on stable ART. METHODS: This was a longitudinal prospective study of PLWH from the GEPPO cohort. At the beginning of the observational period, participants were INSTI-naives (INSTI-n). During follow-up, they were divided in two groups: INSTI-n vs dolutegravir-switchers (DTG-s) with no further change in ART...
February 23, 2021: AIDS
Constance Mubekapi-Musadaidzwa, Dillon Wademan, Neshaan Peton, Portia Hendricks, Gabriela Carolus, Rheiner Mbaezue, Karen F Kelley, James Kruger, Karen Jennings, Nelis Grobbelaar, Francoise Louis, Nulda Beyers, Helen Ayles, Sarah Fidler, Richard Hayes, Peter Bock, Graeme Hoddinott
Background : The HPTN 071 (PopART) trial implemented universal test and treat (UTT) in three clinics in the Western Cape, South Africa at a time when antiretroviral treatment (ART) was only offered by CD4 threshold and World Health Organization clinical staging. This required a concomitant shift in the way health workers communicated ART initiation messages. We provide insight into front-line ART initiation communication pre-national policy shift. Method : The design of this study was exploratory with a case descriptive analysis of ART initiation in three clinics...
February 26, 2021: African Journal of AIDS Research: AJAR
Linda J Koenig, Cynthia M Lyles, Darrel Higa, Mary M Mullins, Theresa A Sipe
OBJECTIVE: Research synthesis, through qualitative or quantitative systematic reviews, allows for integrating results of primary research to improve public health. We examined more than 2 decades of work in HIV prevention by the Centers for Disease Control and Prevention's (CDC's) HIV/AIDS Prevention Research Synthesis (PRS) Project. We describe the context and contributions of research synthesis, including systematic reviews and meta-analyses, through the experience of the PRS Project...
February 26, 2021: Public Health Reports
Faith E Fletcher, Ndidiamaka Amutah-Onukagha, Julie Attys, Whitney S Rice
Black women living with HIV (BWLWH) contend with injuries of injustice, which manifest in restricted reproductive autonomy and decision-making power in social and medical settings. Mitigating threats to reproductive autonomy calls for innovations that consider patients' needs and offer insights on how historically situated marginalization influences today's institutional, political, and economic systems and shapes reproductive decision making. In addition to cross-disciplinary expertise and collaboration, integrating structural competency into reproductive health care requires demonstrating respect for the autonomy, lived experiences, and preferences of BWLWH...
February 1, 2021: AMA Journal of Ethics
Anuradha Subramanian, Ayush Mohan, Sachin Kataria, Rajeshwari Krishnan
The National Programme in India provides free third-line antiretroviral treatment (ART) for all people living with HIV (PLHIV). Data of 232 PLHIV initiated on third-line ART from a single center in New Delhi were retrospectively analyzed for virological suppression at 6 and 12 months, factors predicting nonresponse, retention in care, and mortality from June 2016 till December 2019. The mean age at third-line ART initiation was 39.54 ± 11.08 years, 71.5% were males, and 55.02% had HIV duration of >10 years...
February 24, 2021: AIDS Patient Care and STDs
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