David T Nguyen, Thomas C Larsen, Muyang Wang, Russel H Knutsen, Zhihong Yang, Eric E Bennett, Dumitru Mazilu, Zu-Xi Yu, Xi Tao, Danielle R Donahue, Ahmed Gharib, Christopher K E Bleck, Joel Moss, Alan T Remaley, Beth A Kozel, Han Wen
In pathology protocols, a tissue block, such as one containing a mouse brain or a biopsy sample from a patient, can produce several hundred thin sections. Substantial time may be required to analyze all sections. In cases of uncertainty regarding which sections to focus on, non-invasive scout imaging of intact blocks can help in guiding the pathology procedure. The scouting step is ideally done in a time window of minutes without special sample preparation that may interfere with the pathology procedures. The challenge is to obtain some visibility of unstained tissue structures at sub-10 μm resolution...
January 22, 2021: Journal of Microscopy