Mesenchymal cell transplantation

Yong Yao, Liang Yang, Li-Feng Feng, Zhi-Wei Yue, Nian-Huan Zhao, Zongjin Li, Zuo-Xiang He
BACKGROUND: Due to the low survival rate of cell transplantation, stem cell has not been widely used in clinical treatment of acute myocardial infarction (AMI). In this study, we immobilized the C domain peptide of insulin-like growth factor-1 on chitosan (CS-IGF-1C) to obtain bioactive hydrogel. The purpose was to investigate whether CS-IGF-1C hydrogel incorporated with human placenta-derived mesenchymal stem cells (hP-MSCs) can boost the survival of hP-MSCs and enhance their therapeutic effects...
March 26, 2020: Stem Cell Research & Therapy
Lidiane Maria Boldrini-Leite, Pedro Vicente Michelotto, Sérgio Adriane Bezerra de Moura, Luiz Guilherme Achcar Capriglione, Fernanda Cristina Mendes Barussi, Felipe Yukio Ishikawa Fragoso, Alexandra Cristina Senegaglia, Paulo Roberto Slud Brofman
Mesenchymal stem cell (MSC) research has demonstrated the potential of these cells to modulate lung inflammatory processes and tissue repair; however, the underlying mechanisms and treatment durability remain unknown. Here, we investigated the therapeutic potential of human bone marrow-derived MSCs in the inflammatory process and pulmonary remodeling of asthmatic BALB/c mice up to 14 d after transplantation. Our study used ovalbumin to induce allergic asthma in male BALB/c mice. MSCs were injected intratracheally in the asthma groups...
January 2020: Cell Transplantation
Yajun Zhao, E Xiao, Wanqi Lv, Xian Dong, Linhai He, Yixiang Wang, Yi Zhang
Mesenchymal stem cells (MSCs) possess promising potential in tissue engineering and regenerative medicine. Previous studies demonstrated that spheroid formation of MSCs exhibited improved stemness maintenance and therapeutic potential compared with monolayer culture. To date, various spheroid culture systems have been developed but most of them required low adhesion conditions or special equipment. In this study, we demonstrated that inoculation of dissociated MSCs in TeSR-E8 medium could induce self-assemble spheroid formation in conventional tissue culture polystyrene dishes...
2020: Stem Cells International
Laxmaiah Manchikanti, Christopher J Centeno, Sairam Atluri, Sheri L Albers, Shane Shapiro, Gerard A Malanga, Alaa Abd-Elsayed, Mairin Jerome, Joshua A Hirsch, Alan D Kaye, Steve M Aydin, Douglas Beall, Don Buford, Joanne Borg-Stein, Ricardo M Buenaventura, Joseph A Cabaret, Aaron K Calodney, Kenneth D Candido, Cameron Cartier, Richard Latchaw, Sudhir Diwan, Ehren Dodson, Zachary Fausel, Michael Fredericson, Christopher G Gharibo, Mayank Gupta, Adam M Kaye, Nebojsa Nick Knezevic, Radomir Kosanovic, Matthew Lucas, Maanasa V Manchikanti, R Amadeus Mason, Kenneth Mautner, Samuel Murala, Annu Navani, Vidyasagar Pampati, Sarah Pastoriza, Ramarao Pasupuleti, Cyril Philip, Mahendra R Sanapati, Theodore Sand, Rinoo V Shah, Amol Soin, Ian Stemper, Bradley W Wargo, Philippe Hernigou
BACKGROUND: The use of bone marrow concentrate (BMC) for treatment of musculoskeletal disorders has become increasingly popular over the last several years, as technology has improved along with the need for better solutions for these pathologies. The use of cellular tissue raises a number of issues regarding the US Food and Drug Administration's (FDA) regulation in classifying these treatments as a drug versus just autologous tissue transplantation. In the case of BMC in musculoskeletal and spine care, this determination will likely hinge on whether BMC is homologous to the musculoskeletal system and spine...
March 2020: Pain Physician
Ryo Nakamichi, Hiroshi Asahara
Damage to the intervertebral discs (IVDs) occurs due to aging or excessive mechanical stress, causing a series of IVD-related degenerative diseases, such as spinal disc herniation and spondylosis. These IVD-related diseases are difficult to cure, partially because the regeneration ability of IVDs is not sufficient. As a novel strategy for treatment of IVD-related diseases, mesenchymal stem cell transplantation to the damaged discs has been reported in animal studies. To further develop and improve this approach, it is necessary to gain a better understanding of the molecular network regulating IVD development by critical transcription factors...
March 2020: JOR Spine
Ai-Tong Wang, Qing-Fang Zhang, Nuo-Xin Wang, Chang-Yin Yu, Ru-Ming Liu, Yi Luo, Yu-Jie Zhao, Jian-Hui Xiao
Osteoarthritis (OA) is one of the most common refractory degenerative articular cartilage diseases. Human amniotic mesenchymal cells (hAMSCs) have emerged as a promising stem cell source for cartilage repair, and hyaluronic acid (HA) has proven to be a versatile regulator for stem cell transplantation. Herein, an effective and straightforward intra-articular injection therapy using a cocktail of hAMSCs and HA was developed to treat knee OA in a rat model. The injured cartilage was remarkably regenerated, yielding results comparable to normal cartilage levels after 56 days of treatment...
2020: Frontiers in Bioengineering and Biotechnology
Hui Wang, Yuan Tian, Xin Li, Meijia Yang, Ying Yan
BACKGROUND: Cell transplantation is a promising treatment for the patients with end-stage liver diseases. Stem cells derived hepatocyte-like cells (HLCs) attenuated liver injury upon transplantation in animal models for liver fibrosis. However, only a small portion of the transplanted cells propagated in the recipient liver. AIM: We hypothesized that the efficiency of cell therapy could be improved by transplanting amniotic mesenchymal stem cells (AMSCs) derived HLCs along with human umbilical vein endothelial cells (HUVECs) and undifferentiated AMSCs...
2020: International Journal of Physiology, Pathophysiology and Pharmacology
Chuan Yu, Kun Yang, Xuxia Meng, Bowen Cao, Fenglei Wang
Diabetic retinopathy (DR) is the common and important cause for visual impairment and blindness in working-aged people. Microangiopathy and inflammatory reactions are the key components of DR. Recently, long non-coding RNA myocardial infarction-associated transcript (MIAT) has emerged as a vital player in regulation for inflammatory processes and microvascular dysfunction. Additionally, cell-based therapy provides a potential option for the treatment of DR. The anti-inflammatory effects and repair therapy of mesenchymal stem cells (MSCs) have been paid more attention...
2020: International Journal of Medical Sciences
Wu-Sheng Deng, Ke Ma, Bing Liang, Xiao-Yin Liu, Hui-You Xu, Jian Zhang, Heng-Yuan Shi, Hong-Tao Sun, Xu-Yi Chen, Sai Zhang
Currently, there is no effective strategy to promote functional recovery after a spinal cord injury. Collagen scaffolds can not only provide support and guidance for axonal regeneration, but can also serve as a bridge for nerve regeneration at the injury site. They can additionally be used as carriers to retain mesenchymal stem cells at the injury site to enhance their effectiveness. Hence, we hypothesized that transplanting human umbilical cord-mesenchymal stem cells on collagen scaffolds would enhance healing following acute complete spinal cord injury...
September 2020: Neural Regeneration Research
Rizwan Malik, Fabrice A Darche, Rasmus Rivinius, Anja Seckinger, Ulf Krause, Michael Koenen, Dierk Thomas, Hugo A Katus, Patrick A Schweizer
Engraftment and functional integration of stem cells or stem cell-derived cells within cardiac tissue is an important prerequisite for cell replacement therapy aiming at the treatment of heart disease. Recently, a novel intravenous approach for application of mesenchymal stromal cells (MSCs) to cardiac sites has been established using radiofrequency catheter ablation (RFCA)-guided targeting, bypassing the need for open chest surgery or direct myocardial cell injection. However, little is known about the quantitative efficacy and longevity of this strategy...
January 2020: Cell Transplantation
Zhuowei Gao, Guanghui Deng, Yunjia Li, Huacong Huang, Xuegang Sun, Hao Shi, Xiaofen Yao, Lei Gao, Yongle Ju, Min Luo
Actinidia chinensis Planch (ACP) was the kiwifruit plant Chinese kiwifruit Actinidia chinensis Planch Root, which had been approved to be an anti-tumor drug widespread in clinical. However, the specific mechanism of ACP in resistance to gastric cancer remained unclear. Therefore, our study was dedicated to investigate the anti-proliferation and anti-migration effects of ACP on gastric cancer cells and its molecular mechanisms. Firstly, we utilized HPLC-MS to analyze the composition of ACP decoction, the results showed that ACP contained two main anti-tumor components, Ursolic acid and Oleanolic acid...
March 20, 2020: Biomedicine & Pharmacotherapy
Daisong Wang, Jingqiang Wang, Lanyue Bai, Hong Pan, Hua Feng, Hans Clevers, Yi Arial Zeng
It has generally proven challenging to produce functional β cells in vitro. Here, we describe a previously unidentified protein C receptor positive (Procr+ ) cell population in adult mouse pancreas through single-cell RNA sequencing (scRNA-seq). The cells reside in islets, do not express differentiation markers, and feature epithelial-to-mesenchymal transition characteristics. By genetic lineage tracing, Procr+ islet cells undergo clonal expansion and generate all four endocrine cell types during adult homeostasis...
March 19, 2020: Cell
Yi Sui, Siqi Zhang, Yongliang Li, Xin Zhang, Waner Hu, Yanrui Feng, Jingwei Xiong, Yuanyuan Zhang, Shicheng Wei
BACKGROUND: Organ replacement regenerative therapy based on human adult stem cells may be effective for salivary gland hypofunction. However, the generated tissues are immature because the signaling factors that induce the differentiation of human salivary gland stem cells into salivary glands are unknown. METHODS: Isolated human submandibular gland stem/progenitor cells (hSMGepiS/PCs) were characterized and three-dimensionally (3D) cultured to generate organoids and further induced by fibroblast growth factor 10 (FGF10) in vitro...
March 20, 2020: Stem Cell Research & Therapy
Naoki Ishiuchi, Ayumu Nakashima, Shigehiro Doi, Ken Yoshida, Satoshi Maeda, Ryo Kanai, Yumi Yamada, Takeshi Ike, Toshiki Doi, Yukio Kato, Takao Masaki
BACKGROUND: Mesenchymal stem cells (MSCs) have been reported to promote the regeneration of injured tissue via their paracrine abilities, which are enhanced by hypoxic preconditioning. In this study, we examined the therapeutic efficacy of hypoxia-preconditioned MSCs on renal fibrosis and inflammation in rats with ischemia-reperfusion injury (IRI). METHODS: MSCs derived from rats and humans were incubated in 1% O2 conditions (1%O2 MSCs) for 24 h. After IRI, 1%O2 MSCs or MSCs cultured under normoxic conditions (21%O2 MSCs) were injected through the abdominal aorta...
March 20, 2020: Stem Cell Research & Therapy
Yimei Hong, Haiwei He, Guojun Jiang, Hao Zhang, Wuyuan Tao, Yue Ding, Dongsheng Yuan, Jing Liu, Huimin Fan, Fang Lin, Xiaoting Liang, Xin Li, Yuelin Zhang
Aging impairs the functions of human mesenchymal stem cells (MSCs), thereby severely reducing their beneficial effects on myocardial infarction (MI). MicroRNAs (miRNAs) play crucial roles in regulating the senescence of MSCs; however, the underlying mechanisms remain unclear. Here, we investigated the significance of miR-155-5p in regulating MSC senescence and whether inhibition of miR-155-5p could rejuvenate aged MSCs (AMSCs) to enhance their therapeutic efficacy for MI. Young MSCs (YMSCs) and AMSCs were isolated from young and aged donors, respectively...
March 20, 2020: Aging Cell
Huimin Fang, Chao Luo, Shaokai Liu, Muran Zhou, Yuyang Zeng, Jinfei Hou, Lifeng Chen, Shan Mou, Jiaming Sun, Zhenxing Wang
The survival of transplanted cells and tissues in bone regeneration requires a microenvironment with a vibrant vascular network. A tissue engineering chamber can provide this in vivo . However, the commonly used silicone chamber is biologically inert and can cause rejection reactions and fibrous capsule. Studies have revealed that collagen is highly biocompatible and graphene oxide (GO) could regulate osteogenic activity in vivo . Besides, GO can be cross-linked with natural biodegradable polymers to construct scaffolds...
2020: Theranostics
Jaiza Frias Pedroso, Vanessa de Souza Valim, Annelise Pezzi, Juliana Monteiro Furlan, Gabriele Lenhart, Filipe Sehn, Bruna Zambonato, Alice Dahmer Gonçalves, Ianaê Wilke, Bruna Amorin, Maria Aperecida da Silva, Fabiane Spagnol Pedrazzani, Lucia Mariano da Rocha Silla
NK cells have been seen as potential agents in adoptive immunotherapy for cancer. The main challenge for the success of this approach is to obtain a great quantity of activated NK cells for adoptive transfer. The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. MSCs were obtained from the bone marrow (BM) cells remaining in the bag and filter used in the transplantation of hematopoietic stem cells. NK cells were obtained from peripheral blood (PB) of healthy volunteers...
March 19, 2020: Molecular Biotechnology
Ewa Kruminis-Kaszkiel, Adam Osowski, Ewa Bejer-Oleńska, Mariusz Dziekoński, Joanna Wojtkiewicz
The transplantation of neural stem cells (NSCs) capable of regenerating to the cells of the central nervous system (CNS) is a promising strategy in the treatment of CNS diseases and injury. As previous studies have highlighted mesenchymal stem cells (MSCs) as a source of NSCs, this study aimed to develop a feasible, efficient, and reproducible method for the neural induction of MSCs isolated from Wharton's jelly (hWJ-MSCs). We induced neural differentiation in a monolayer culture using epidermal growth factor, basic fibroblast growth factor, N2, and B27 supplements...
March 17, 2020: Cells
Takeshi Hori, Osamu Kurosawa, Kohei Ishihara, Taro Mizuta, Hiroo Iwata
Cell sheet engineering has become important in a variety of fields, including regenerative medicine and transplantation. Our research group previously developed micromesh cultures that enable cells to form a cell sheet on a microstructured mesh sheet. Here, we present a more usable micromesh culture and devices that make it possible, aiming for widespread use. The devices are mainly constituted of a polyester micromesh sheet and three-dimensional (3D)-printed simple frames that fix the mesh sheet on it. Cells such as fibroblast Tig-1-20 cells, hepatoma HepG2 cells, or mesenchymal stem cells (MSC) were easily seeded on the polyester mesh sheet in the device and cultured for 16 days, which was followed by the formation of a 100-400-μm-thick cell sheet...
March 2020: Tissue Engineering. Part C, Methods
Eleonora Russo, Jea-Young Lee, Hung Nguyen, Simona Corrao, Rita Anzalone, Giampiero La Rocca, Cesar V Borlongan
Human umbilical cord mesenchymal stem cells (hUC-MSCs) are a pivotal source of therapeutically active cells for regenerative medicine due to their multipotent differentiation potential, immunomodulatory and anti-inflammatory proprieties, as well as logistical collection advantages without ethical concerns. However, it remains poorly understood whether MSCs from different compartments of the human umbilical cord are therapeutically superior than others. In this study, MSCs were isolated from Wharton's jelly (WJ-MSCs), perivascular region (PV-MSCs) and cord lining (CL-MSCs) of hUC...
March 17, 2020: Stem cell reviews and reports
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