Christina M Osborne, Charles Langelier, Jack Kamm, Kayla Williamson, Lilliam Ambroggio, Ron W Reeder, Christopher Locandro, J Kirk Harris, Brandie D Wagner, Aline B Maddux, Saharai Caldera, Amy Lyden, Victoria Soesanto, Eric A F Simões, Matthew K Leroue, Todd C Carpenter, Mark W Hall, Athena F Zuppa, Joseph A Carcillo, Kathleen L Meert, Murray M Pollack, Patrick S McQuillen, Daniel A Notterman, Joseph DeRisi, Peter M Mourani
OBJECTIVES: Viral lower respiratory tract infection (vLRTI) contributes to substantial morbidity and mortality in children. Diagnosis is typically confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) of nasopharyngeal specimens in hospitalized patients; however, it is unknown whether nasopharyngeal detection accurately reflects presence of virus in the lower respiratory tract (LRT). This study evaluates agreement between viral detection from nasopharyngeal specimens by RT-PCR compared with metagenomic next-generation RNA sequencing (RNA-Seq) from tracheal aspirates (TAs)...
September 21, 2023: Pediatric Critical Care Medicine