Aml A Emam, Ibrahim A Naguib, Eman S Hassan, Eglal A Abdelaleem
BACKGROUND: Felodipine is a calcium channel blocker used together with metoprolol succinate for treatment of hypertension. OBJECTIVE: Two chromatographic methods were developed for simultaneous determination of felodipine (FEL) and metoprolol succinate (MET), and their major metabolites, dehydrofelodipine and metoprolol acid, respectively. METHODS: The first method was RP-HPLC which comprised separation of the studied components by gradient elution using a Phenomenex C8 column and a mobile phase composed of water (adjusted to pH 3...
July 1, 2020: Journal of AOAC International
Matilda Shackley, Yue Ma, Edward W Tate, Alastair J H Brown, Gary Frost, Aylin C Hanyaloglu
The short chain fatty acids (SCFAs) acetate, butyrate and propionate, are produced by fermentation of non-digestible carbohydrates by the gut microbiota and regulate appetite, adiposity, metabolism, glycemic control, and immunity. SCFAs act at two distinct G protein coupled receptors (GPCRs), FFAR2 and FFAR3 and are expressed in intestinal enteroendocrine cells (EECs), where they mediate anorectic gut hormone release. EECs also express other GPCRs that act as nutrient sensors, thus SCFAs may elicit some of their health-promoting effects by altering GPCR expression in EECs and enhance gut sensitivity to dietary molecules...
2020: Frontiers in Nutrition
Xianghong Luo, Hao Fu, Chaoqun Xu, Yang Dong, Zhihua Wu, Dongxiao Li, Ying Sun, Ming Shen, Liting Wang, Zhaojun Li, Yourong Duan
Atherosclerosis (AS) is one of the leading causes of vascular disease, producing high morbidity and mortality in many countries. Autophagy plays an important role when cells are facing serious circumstances, such as oxidative stress induced by Ox-LDL (oxidized low-density lipoprotein). Recent studies have revealed that DEX (dexamethasone acetate) and RAPA (rapamycin) exhibit efficient AS therapeutic ability by protecting endothelial cells and killing foam cells, respectively. Herein, we hypothesize that combining DEX and RAPA together in a specific nanocarrier system can achieve better AS therapy while limiting harmful effects...
June 1, 2020: Journal of Biomedical Nanotechnology
Fei Su, Yuyou Yang
AIMS: Various applications of microbially induced carbonate precipitation (MICP) has been proposed. However, most studies use cultured pure strains to obtain MICP, ignoring advantages of microbial consortia. The aims of this study were to: 1) test the feasibility of a microbial consortium to produce MICP; 2) identify functional microorganisms and their relationship; 3) explain the MICP mechanism; 4) propose a way of applying the MICP technique to soil media. METHODS AND RESULTS: Anaerobic sludge was used as the source of the microbial consortium...
November 13, 2020: Journal of Applied Microbiology
Haiyang Wang, Toshinari Maeda, Toshiki Miyazaki
Polymethyl methacrylate (PMMA)-based bone cement is a popular biomaterial used for fixation of artificial joints. A next-generation bone cement having bone-bonding ability, i.e., bioactivity and antibacterial property is desired. We previously revealed that PMMA cement added with 2-(tert-butylamino)ethyl methacrylate, γ-methacryloxypropyltrimethoxysilane and calcium acetate showed in vitro bioactivity and antibacterial activity. This cement contains calcium acetate at 20% of the powder component. Lower content of the calcium acetate is preferable, because the release of a lot of calcium salt may degrade mechanical properties in the body environment...
November 6, 2020: Materials
Hao Li, Yongmin Shi
In most oilfields, acid fracturing is widely used for oil production. Understanding the relationship between the individual factors (i.e., carbonate rock types, acid rock reaction kinetics, and deterioration of rock mechanical properties) can provide practical guidelines that can be used for the design and optimization of acid fracturing operation. This paper takes hydrochloric acid, acetic acid, and citric acid as the main research objects and carries out acidification experiments on sandstone in Changqing Oilfield, China...
November 3, 2020: ACS Omega
Sudipta Kumar Hazra, Tanmay Sarkar, Molla Salauddin, Hassan I Sheikh, Siddhartha Pati, Runu Chakraborty
Aegle marmelos (L.) is a seasonal fruit that contains significant amounts of bioactives like, phenolic acids (gallic acids, 2,3-dihydroxy benzoic acid, chlorogenic acid, p -coumaric acid, vanillic acid), flavonoid (rutin), organic acids (oxalic acid, tartaric acid, malic acid, lactic acid, acetic acid, citric acid, propionic acid, succinic acid, fumaric acid), vitamin C, vitamin B group (thiamine, niacin, pyridoxine, pantothenic acid, biotin, cobalamins, riboflavin), tocopherols (α - tocopherol, β - tocopherol, γ - tocopherol, δ - tocopherol), carotenes (α - carotene, β - carotene, γ - carotene, δ - carotene) and also rich in essential minerals (potassium, calcium, phosphorus, sodium, iron, copper, manganese)...
October 2020: Heliyon
Samuel Maritim, Pierre Boulas, Yiqing Lin
An understanding of the factors that affect liposome size, drug loading, stability and drug release is critical for the rational design of liposomes with desired pharmacokinetics and biodistribution. This article presents a report on the formulation and characterization of BIIB093 liposomes as well as a detailed analysis of the influence of formulation methods and parameters on encapsulation efficiency, liposome size, charge (zeta potential, ZP), polydispersity index (PDI), and drug release. PEGylated liposomes containing BIIB093 were made using ethanol injection and calcium acetate remote loading...
November 5, 2020: International Journal of Pharmaceutics
Yasmine Zahouani, Khemais Ben Rhouma, Kamel Kacem, Hichem Sebai, Mohsen Sakly
We investigate the antioxidant activity and protective effects of the aqueous leaf extract of Pistacia lentiscus (AELPL) against ulcerative colitis induced by acetic acid infusion through the rectum in Wistar rats. Phytochemical analyses allowed the identification of numerous phenolic compounds in P. lentiscus leaves such as flavonoids (isoquercetin and luterolin), flavonols (catechin, rutin, and kaempferol), phenolic acids (ellagic and dicaffeoylquinic), and tanins. Acetic acid exposure induced macroscopic colonic mucosal lesions with hemorrhage, congestion, edema, and the development of an expected oxidative stress state revealed by an increase in lipoperoxidation and carbonylation of proteins and a decrease in sulfhydryl (SH) group levels and antioxidant enzyme activities such as superoxide dismutase, catalase, glutathione-S-peroxidase, and glutathione transferase, as well as an increase in the inflammatory cytokine, interleukin-6, in the colon and plasma...
November 6, 2020: Journal of Medicinal Food
Fei Shao, Lei Yu, Yang Zhang, Chuanfeng An, Haoyue Zhang, Yujie Zhang, Yi Xiong, Huanan Wang
Microfluidics-based alginate microgels have shown great potential to encapsulate cells in a high-throughput and controllable manner. However, cell viability and biological functions are substantially compromised due to the harsh conditions for gelation, which remains a major challenge for cell encapsulation. Herein, we presented an efficient and biocompatible method by on-chip triggered gelation to generate microfluidic alginate microgels for single-cell encapsulation. Two calcium complexes of calcium-ethylenediaminetetraacetic acid (Ca-EDTA) and calcium-nitrilotriacetic (Ca-NTA) as crosslinkers for triggered gelation of alginate were compared and investigated for feasible application...
2020: Frontiers in Bioengineering and Biotechnology
Rawiporn Amornloetwattana, Robert C Robinson, Hannadige Sasimali Madusanka Soysa, Bert van den Berg, Wipa Suginta
Serratia marcescens is an opportunistic pathogen that commonly causes hospital-acquired infections and can utilize chitin-enriched nutrients as an alternative energy source. This study reports the identification of a chitoporin (ChiP), termed SmChiP, from the outer membrane of S. marcescens. Sequence alignment with genetically characterized ChiPs suggests that SmChiP is more closely related to the monomeric EcChiP from Escherichia coli than to the trimeric VhChiP from Vibrio campbellii. A single crystal of SmChiP grown under the condition 22%(w/v) PEG 8000, 0...
November 1, 2020: Acta Crystallographica. Section F, Structural Biology Communications
Ping Zhao, Jia Liu, Qian Ming, Di Tian, Jingwen He, Ziwei Yang, Jinhua Shen, Qing-Hua Liu, Xinzhou Yang
Background: Asthma is one of the main intractable diseases recognized by the international medical community. The current widely used bronchodilators for asthma-β2-adrenal receptor agonists-have limited therapeutic effects, necessitating the development of novel antiasthma drugs with increased efficacy and fewer adverse effects. In this study, we investigated the relaxant effects and underlying mechanism of an ethyl acetate extract from dandelion (EAED) on mouse airway smooth muscle...
2020: Cell & Bioscience
Yun-Mi Kang, Minho Lee, Hyo-Jin An
BACKGROUND: Oleanolic acid (OA) is an active compound found in a variety of medicinal herbs and plants. Though OA has been widely attributed with a variety of biological activities, studies focused on its anti-allergic inflammation properties are insufficient. PURPOSE: Given the rapid increase in allergic diseases and the lack of fundamental treatment options, this study aimed to find a safe and effective therapy for allergic disorders. METHODS: We evaluated the inhibitory effect of OA on allergic inflammatory response and the possible mechanisms underlying the effect using phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cell (HMC)-1, and a mouse model of compound 48/80-induced anaphylactic shock...
October 15, 2020: Phytomedicine
Ali Aldalbahi, Mehrez El-Naggar, Tawfik Khattab, Meram Abdelrahman, Mostafizur Rahaman, Abdulaziz Alrehaili, Mohamed El-Newehy
: Novel ecofriendly adsorbents, cellulose acetate/graphene oxide (CA-GO) nanocomposite, were prepared from sugarcane bagasse agro-waste for removing Ni2+ ions from wastewater. Graphene oxide (GO) was prepared by the oxidation of sugarcane bagasse using ferrocene under air atmosphere. Cellulose acetate (CA) was also prepared from sugarcane bagasse by extraction of cellulose through a successive treatments with sulfuric acid (10% v/v), sodium hydroxide (5% w/v), ethylenediaminetetraacetic acid, and hydrogen peroxide, and finally , followed by acetylation...
October 27, 2020: Polymers
Ilnar Shaidullov, Elizaveta Ermakova, Aisylu Gaifullina, Anna Mosshammer, Aleksey Yakovlev, Thomas M Weiger, Anton Hermann, Guzel Sitdikova
Acetaldehyde and acetic acid/acetate, the active metabolites of alcohol (ethanol, EtOH), generate actions of their own ranging from behavioral, physiological, to pathological/cancerogenic effects. EtOH and acetaldehyde have been studied to some depth, whereas the effects of acetic acid have been less well explored. In this study, we investigated the effect of acetic acid on big conductance calcium-activated potassium (BK) channels present in GH3 rat pituitary tumor cells in more detail. In whole cell voltage clamp recordings, extracellular application of acetic acid increased total outward currents in a dose-dependent manner...
October 28, 2020: Pflügers Archiv: European Journal of Physiology
Gala Freixer, Khaoula Zekri-Nechar, José J Zamorano-León, Carlos Hugo-Martínez, Nora V Butta, Elena Monzón, María-José Recio, Manel Giner, Antonio López-Farré
Long-term therapy with low Aspirin (ASA) dose is basis to prevent thrombotic acute events. However, the anti-platelet mechanisms of ASA remain not completely known. The aim was to analyze if in vitro exposure of human megakaryocytes to low ASA concentration may alter the apoptotic features of the newly formed platelets. Cultured Meg-01 cells, a human megakaryoblastic cell line, were stimulated to form platelets with 10 nmol/L phorbol 12-myristate-13-acetate (PMA) in the presence and absence of ASA (0.33 mmol/L)...
October 28, 2020: Platelets
Larry A Greenbaum, Nikola Jeck, Günter Klaus, Marc Fila, Cristina Stoica, Sahar Fathallah-Shaykh, Ana Paredes, Larysa Wickman, Raoul Nelson, Rita D Swinford, Carolyn Larkins Abitbol, Mihaela Balgradean, Augustina Jankauskiene, Amandine Perrin, Milica Enoiu, Sun-Young Ahn
BACKGROUND: Pediatric patients with advanced chronic kidney disease (CKD) are often prescribed oral phosphate binders (PBs) for the management of hyperphosphatemia. However, available PBs have limitations, including unfavorable tolerability and safety. METHODS: This phase 3, multicenter, randomized, open-label study investigated safety and efficacy of sucroferric oxyhydroxide (SFOH) in pediatric and adolescent subjects with CKD and hyperphosphatemia. Subjects were randomized to SFOH or calcium acetate (CaAc) for a 10-week dose titration (stage 1), followed by a 24-week safety extension (stage 2)...
October 27, 2020: Pediatric Nephrology
Julie-Anne Lemay, Mai Yamamoto, Zachary Kroezen, Meera Shanmuganathan, Ritchie Ly, Lara Hart, Nikhil Pai, Philip Britz-McKibbin
Short-chain fatty acids (SCFAs) and electrolytes are major constituents of human feces involved in maintaining gastrointestinal homeostasis that underlie complex diet, host and microbiome interactions. Reliable quantification of SCFAs and electrolytes is challenging given the heterogeneity of stool specimens from pediatric patients with diarrhea-predominate inflammatory bowel disease (IBD). Herein, we introduce two validated methods for determination of 3 SCFAs and 5 electrolytes consistently quantified from fecal extracts when using capillary electrophoresis with indirect UV detection (CE-iUV), where concentrations are normalized to total dried weight (mmol/kg d...
October 3, 2020: Journal of Pharmaceutical and Biomedical Analysis
Tahani Osman Issa, Ahmed Ibrahim Mohamed Ahmed, Yahya Sulieman Mohamed, Sakina Yagi, Abdelrafie Mohamed Makhawi, Tarig Osman Khider
The present study aimed to investigate the physiochemical activities of Senna occidentalis (Linn) roots and phytochemicals as insecticidal (ethyl acetate and methanol) and antidiabetic (ethanolic extract) activities. Physicochemical properties were carried out by using Association of Official Analytical Chemist methods; thin layer chromatography was carried out according to the Stahl method. Larvicidal activity and LD50 were studied against the third instar of Culex quinquefasciatus mosquito larvae to detect and extract toxicity...
2020: Biochemistry Research International
Yi-Fen Chiang, Hui-Chih Hung, Hsin-Yuan Chen, Ko-Chieh Huang, Po-Han Lin, Jen-Yun Chang, Tsui-Chin Huang, Shih-Min Hsia
Primary dysmenorrhea is a common occurrence in adolescent women and is a type of chronic inflammation. Dysmenorrhea is due to an increase in oxidative stress, which increases cyclooxygenase-2 (COX-2) expression, increases the concentration of prostaglandin F2α (PGF2α), and increases the calcium concentration in uterine smooth muscle, causing excessive uterine contractions and pain. The polyphenolic compound oleocanthal (OC) in extra virgin olive oil (EVOO) has been shown to have an anti-inflammatory and antioxidant effect...
September 30, 2020: Nutrients
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