Wanglong Chu, Fen Zhang, Xiuping Zeng, Fangtao He, Guanyan Shang, Tao Guo, Qingfang Wang, Jianfu Wu, Tongjing Li, Zhen Zhong Zhong, Xiao Liang, Junyuan Hu, Muyun Liu
BACKGROUND: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) hold great therapeutic potential in regenerative medicine. Therefore, it is crucial to establish a Good Manufacturing Practice (GMP)-compliant methodology for the isolation and culture of WJ-MSCs. Through comprehensive research, encompassing laboratory-scale experiments to pilot-scale studies, we aimed to develop standardized protocols ensuring the high yield and quality of WJ-MSCs manufacturing. METHODS: Firstly, optimization of parameters for the enzymatic digestion method used to isolate WJ-MSCs was conducted...
May 3, 2024: Stem Cell Research & Therapy