Claudia P Marin, Geovana L Santana, Meghan Robinson, Stephanie M Willerth, Murilo C Crovace, Edgar D Zanotto
This study evaluatedthe gene expression profile ofthe human adipose-derived stem cells (hASCs)grown onthe Biosilicate®/F18 glass (BioS-2P/F18) scaffolds. hASCswere cultured using the osteogenic medium (control),the scaffolds, and their ionic extract. We observed that ALP activity was higher inhASCs grown onthe BioS-2P/F18 scaffolds than inhASCs cultured withtheionic extractorthe osteogenic mediumon day 14. Moreover, the dissolution product group and the control exhibited deposited calcium, which peaked on day 21...
October 18, 2020: Journal of Biomedical Materials Research. Part A
Xudong Liu, Yuchao Zhang, Liangyu Liu, Sijie Zhu
Ethanol has a complex effect on the cardiovascular system in humans, but the systemic effects of ethanol to endothelial cells were rarely investigated. In this study, we exposed human umbilical vein endothelial cells (HUVECs) to 5- or 50-mM ethanol and performed transcriptomics to investigate the systemic effects of ethanol. While these concentrations of ethanol did not significantly affect HUVEC viability, 5-mM ethanol significantly upregulated and downregulated 59 and 73 genes, respectively, whereas 50-mM ethanol significantly upregulated and downregulated 50 and 80 genes, respectively...
October 15, 2020: Journal of Applied Toxicology: JAT
Hiroki Shimomura, Reona Okada, Takako Tanaka, Yuto Hozaka, Masumi Wada, Shogo Moriya, Tetsuya Idichi, Yoshiaki Kita, Hiroshi Kurahara, Takao Ohtsuka, Naohiko Seki
Our recent studies have implicated some passenger strands of miRNAs in the molecular pathogenesis of human cancers. Analysis of the microRNA (miRNA) expression signature in pancreatic ductal adenocarcinoma (PDAC) has shown that levels of miR-30a-3p, the passenger strand derived from pre-mir-30a, are significantly downregulated in PDAC tissues. This study aimed to identify the oncogenes closely involved in PDAC molecular pathogenesis under the regulation of miR-30a-3p. Ectopic expression assays showed that miR-30a-3p expression inhibited the aggressiveness of the PDAC cells, suggesting that miR-30a-3p acts as a tumor-suppressive miRNA in PDAC cells...
September 4, 2020: International Journal of Molecular Sciences
Xiandong Peng, Min Yu, Jiazhou Chen
OBJECTIVE: To identify key genes in ovarian cancer using transcriptome sequencing in two cell lines: MCV152 (benign ovarian epithelial tumour) and SKOV-3 (ovarian serous carcinoma). METHODS: Differentially expressed genes (DEGs) between SKOV-3 and MCV152 were identified. Candidate genes were assessed for enrichment in gene ontology function and Kyoto Encyclopaedia of Genes and Genomes pathway. Candidate gene expression in SKOV-3 and MCV152 cells was validated using Western blots...
September 2020: Journal of International Medical Research
Rofaida Gaballa, Hamdy E A Ali, Mohamed O Mahmoud, Johng S Rhim, Hamed I Ali, Heba F Salem, Mohammad Saleem, Mohamed A Kandeil, Stefan Ambs, Zakaria Y Abd Elmageed
Although integrin alpha 2 subunit (ITGA2) mediates cancer progression and metastasis, its transfer by exosomes has not been investigated in prostate cancer (PCa). We aimed to determine the role of exosomal ITGA2 derived from castration-resistant PCa (CRPC) cells in promoting aggressive phenotypes in androgen receptor (AR)-positive cells. Exosomes were co-incubated with recipient cells and tested for different cellular assays. ITGA2 was enriched in exosomes derived from CRPC cells. Co-culture of AR-positive cells with CRPC-derived exosomes increased their proliferation, migration, and invasion by promoting epithelial-mesenchymal transition, which was reversed via ITGA2 knockdown or inhibition of exosomal uptake by methyl-β-cyclodextrin (MβCD)...
August 15, 2020: Cancers
Li-Bao Gong, Chuang Zhang, Ruo-Xi Yu, Ce Li, Yi-Bo Fan, Yun-Peng Liu, Xiu-Juan Qu
Purpose: To explore the role of FKBP prolyl isomerase 10 ( FKBP10 ) protein in the progression of gastric cancer. Methods: Four independent gastric cancer databases (GSE27342, GSE29272, GSE54129 and TCGA-STAD) were used to identify differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was used to identify the abnormally active pathways in patients with gastric cancer. Univariate Cox regression analysis was used to identify genes with stable prognostic value in gastric cancer patients based on three independent gastric cancer databases (GSE15459, GSE62254, TCGA-STAD)...
2020: OncoTargets and Therapy
Dorina Lauritano, Giulia Moreo, Luisa Limongelli, Annalisa Palmieri, Francesco Carinci
Drug-induced gingival overgrowth may occur after a chronic administration of three classes of systemic drugs: Anticonvulsants, immunosuppressants, and calcium channel blockers. This study aimed to investigate how cyclosporin A and mycophenolate mophetil (immunosuppressive drugs) could interfere with human gingival fibroblasts functions, leading to gingival enlargement. Human gingival fibroblasts derived from the tissue of a 60-year-old female were cultured in a DMEME medium. A stock solution with 1 mg/mL of mycophenolate and 1 mg/mL of cyclosporine were prepared and dissolved in a DMEM medium to prepare a serial dilution at the concentrations of 5000, 2000, 1000, 500, and 100 ng/mL, for both treatments...
July 17, 2020: Biomedicines
Sema S Hakki, Serife Buket Bozkurt, Erdogan E Hakki, Forrest H Nielsen
The effects of boron on the formation and maintenance of mineralized structures at the molecular level are still not clearly defined. Thus, a study was conducted using MC3T3-E1 cells to determine whether boron affected mRNA expressions of genes associated with bone/alveolar bone formation around the teethMC3T3-E1 (clone 4) cells were cultured in media treated with boric acid at concentrations of 0, 0.1, 10, 100, or 1000 ng/ml. Total RNAs of each group were isolated on day 3. Gene expression profiles were determined by using RT2 Profiler PCR micro-array that included 84 genes associated with osteogenic differentiation...
June 27, 2020: Biological Trace Element Research
Tingting Zhu, Ruifang Chen, Jieyu Wang, Huiran Yue, Xin Lu, Jun Li
Background: Deregulation of integrins signaling had been documented to participate in multiple fundamental biological processes, and the aberrant expression of integrin family members were linked to the prognosis of various cancers. However, the role of integrins in predicting progression and prognosis of ovarian cancer patients are still largely elusive. This study is aimed to explore the prognostic values of ITGA and ITGB superfamily members in high grade serous ovarian cancers (HGSOC)...
2020: Cancer Cell International
Lumin Wang, Yi Gao, Xiaoge Zhao, Chen Guo, Xiaofei Wang, Yang Yang, Cong Han, Lingyu Zhao, Yannan Qin, Liying Liu, Chen Huang, Wenjing Wang
OBJECTIVES: HOXD3 is associated with progression of multiple types of cancer. This study aimed to identify the association of YY1 with HOXD3-ITGA2 axis in the progression of hepatocellular carcinoma. MATERIALS AND METHODS: Bioinformatics assay was used to identify the effect of YY1, HOXD3 and ITGA2 expression in HCC tissues. The function of YY1 and HOXD3 in HCCs was determined by qRT-PCR, MTT, apoptosis, Western blotting, colony formation, immunohistochemistry, and wound-healing and transwell invasion assays...
June 17, 2020: Cell Proliferation
Valeriy Shevchenko, Natalia Arnotskaya, Oleg Pak, Aruna Sharma, Hari Shanker Sharma, Yuri Khotimchenko, Andrey Bryukhovetskiy, Igor Bryukhovetskiy
Glioblastoma multiforme (GBM) is the most common primary tumor of the human brain. It is characterized by invasive growth and strong resistance to treatment, and the median survival time of patients is 15 months. The invasive growth of this tumor type is associated with tumor cells with an aggressive phenotype, while its treatment resistance is attributed to cancer stem cells (CSCs). It remains unclear if CSCs have a more invasive nature than differentiated glioblastoma cells (DGCs), and what contribution CSCs make to the aggressive phenotype of GBM...
2020: International Review of Neurobiology
Alin Rai, David W Greening, Rong Xu, Wittaya Suwakulsiri, Richard J Simpson
In localized tumours, basement membrane (BM) prevents invasive outgrowth of tumour cells into surrounding tissues. When carcinomas become invasive, cancer cells either degrade the BM or reprogram the stromal fibroblasts to breach the BM barrier and lead invasion of cancer cells into surrounding tissues in a process called fibroblast-led invasion. However, tumour-derived factors orchestrating fibroblast-led invasion remains poorly understood. Here we show that although early-stage primary colorectal adenocarcinoma (SW480) cells are themselves unable to invade MatrigelTM matrix, they secrete exosomes that reprogram normal fibroblasts to acquire de novo capacity to invade matrix and lead invasion of SW480 cells...
May 21, 2020: Proteomics
Ali Raza Jahejo, Nasir Rajput, Jam Kashif, Dildar Hussain Kalhoro, Sheng Niu, Meng-Li Qiao, Ding Zhang, Muhammad Farhan Qadir, Raza Ali Mangi, Afrasyab Khan, Anam Ahsan, Ajab Khan, Wen-Xia Tian
Tibial dyschondroplasia (TD) is a skeletal deformity disease in broilers that occurs when vascularization in the growth plate (GP) is below normal. Although, blood vessels have been reported to contribute significantly in bone formation. Therefore, in the current study, we have examined the mRNA expression of angiogenesis-related genes in erythrocytes of thiram induced TD chickens by qRT-PCR and performed histopathological analysis to determine regulatory effect of recombinant Glutathione-S-Transferase A3 (rGSTA3) protein in response to the destructive effect of thiram following the injection of rGSTA3 protein...
May 11, 2020: Research in Veterinary Science
Iman Shaheen, Mervat Khorshied, Rasha Abdel-Raouf, Heba Gouda, Dina Kamal, Nelly Abulata, Reham Aboukhalil, Basant Meligy
Sickle cell disease (SCD) is an autosomal recessive hemoglobinopathy characterized by increased cellular adhesiveness. Vaso-occlusion (VOC) is the most prevalent disease complication of SCD that could be altered by genetic factors. L-Selectin and integrin alpha 2 (ITGA2) are 2 adhesion molecules linked to vasculopathy and inflammation. The current study aimed at detecting the prevalence of genetic variants of L-selectin and ITGA2 as possible molecular modulators and novel therapeutic targets in a cohort of pediatric SCD patients...
May 19, 2020: Journal of Pediatric Hematology/oncology
Wenying Yan, Xingyi Liu, Yibo Wang, Shuqing Han, Fan Wang, Xin Liu, Fei Xiao, Guang Hu
Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes of cancer-related death and has an extremely poor prognosis. Thus, identifying new disease-associated genes and targets for PDAC diagnosis and therapy is urgently needed. This requires investigations into the underlying molecular mechanisms of PDAC at both the systems and molecular levels. Herein, we developed a computational method of predicting cancer genes and anticancer drug targets that combined three independent expression microarray datasets of PDAC patients and protein-protein interaction data...
2020: Frontiers in Pharmacology
Shensen Wang, Xinhui Liu, Aamir Ali Khan, Huan Li, Muhammad Tahir, Xinlong Yan, Juan Wang, Hua Huang
Pancreatic cancer (PC) is recognized as the most aggressive and deadliest malignancy because it has the highest mortality of all cancers in humans. Mutations in multiple tumor suppressors and oncogenes have been documented to be involved in pancreatic cancer progression and metastasis. The upregulation of tetraspanin 1 (TSPAN1), a transmembrane protein, has been reportedly observed in many human cancers. However, the role of TSPAN1 and its underlying molecular mechanisms in PC progression have not been fully elucidated...
2020: American Journal of Cancer Research
Anders Sundqvist, Eleftheria Vasilaki, Oleksandr Voytyuk, Yu Bai, Masato Morikawa, Aristidis Moustakas, Kohei Miyazono, Carl-Henrik Heldin, Peter Ten Dijke, Hans van Dam
Activator protein (AP)-1 transcription factors are essential elements of the pro-oncogenic functions of transforming growth factor-β (TGFβ)-SMAD signaling. Here we show that in multiple HER2+ and/or EGFR+ breast cancer cell lines these AP-1-dependent tumorigenic properties of TGFβ critically rely on epidermal growth factor receptor (EGFR) activation and expression of the ΔN isoform of transcriptional regulator p63. EGFR and ΔNp63 enabled and/or potentiated the activation of a subset of TGFβ-inducible invasion/migration-associated genes, e...
April 29, 2020: Oncogene
Yujie Shen, Shikun Dong, Jinhui Liu, Liqing Zhang, Jiacheng Zhang, Han Zhou, Weida Dong
Background: The molecular mechanisms and genetic markers of thyroid cancer are unclear. In this study, we used bioinformatics to screen for key genes and pathways associated with thyroid cancer development and to reveal its potential molecular mechanisms. Methods: The GSE3467, GSE3678, GSE33630, and GSE53157 expression profiles downloaded from the Gene Expression Omnibus database (GEO) contained a total of 164 tissue samples (64 normal thyroid tissue samples and 100 thyroid cancer samples)...
2020: BioMed Research International
Chen Liu, Shengming Deng, Kaizhou Jin, Yitao Gong, He Cheng, Zhiyao Fan, Yunzhen Qian, Qiuyi Huang, Quanxing Ni, Guopei Luo, Xianjun Yu
Carbohydrate antigen 19‑9 (CA19‑9) is the most important biomarker for pancreatic cancer. Approximately 5‑10% of individuals are Lewis antigen negative with scarce secretion of CA19‑9 and fucosylation deficiency. However, the characteristics of Lewis‑negative pancreatic cancer are unidentified. Clinicopathological characteristics of 853 patients with pancreatic cancer were examined. Pancreatic cancer cell lines were sequenced for Lewis status. Morphological and molecular features of pancreatic cancer cells were compared...
February 17, 2020: International Journal of Oncology
Aichun Qin, Qiong Liu, Jingfang Wang
The present study aimed to investigate the roles of Ropivacaine in papillary thyroid cancer (PTC) and identify the possible mechanisms. The expression of integrin alpha-2 (ITGA2) in TC cell lines was tested using Western blotting and RT-qPCR. Subsequently, the level of ITGA2 in human PTC cell line (TPC-1) was measured following intervention with a series of concentrations of Ropivacaine. Then, cell counting kit-8 (CCK-8) assay and colony formation assay were executed for detecting proliferation of cells after transfection with ITGA2 pcDNA3...
April 20, 2020: Drug Development Research
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