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Mitochondrial mass

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https://read.qxmd.com/read/32220024/alleviating-effects-of-reduced-graphene-oxide-against-lead-induced-cytotoxicity-and-oxidative-stress-in-human-alveolar-epithelial-a549-cells
#1
Maqusood Ahamed, Mohd Javed Akhtar, M A Majeed Khan, Hisham A Alhadlaq
Broad application of reduced graphene oxide (rGO) and ubiquitous lead (Pb) pollution may increase the possibility of combined exposure of humans. Information on the combined effects of rGO and Pb in human cells is scarce. This work was designed to explore the potential effects of rGO on Pb-induced toxicity in human alveolar epithelial (A549) cells. Prepared rGO was polycrystalline in nature. The formation of a few layers of visible creases and silky morphology due to high aspect ratio was confirmed. Low level (25 μg/mL) of rGO was not toxic to A549 cells...
March 27, 2020: Journal of Applied Toxicology: JAT
https://read.qxmd.com/read/32212257/prkar1a-deficiency-impedes-hypertrophy-and-reduces-heart-size
#2
Yuening Liu, Peng Xia, Jingrui Chen, W Patricia Bandettini, Lawrence S Kirschner, Constantine A Stratakis, Zhaokang Cheng
Protein kinase A (PKA) activity is pivotal for proper functioning of the human heart, and its dysregulation has been implicated in a variety of cardiac pathologies. PKA regulatory subunit 1α (R1α, encoded by the PRKAR1A gene) is highly expressed in the heart, and controls PKA kinase activity by sequestering PKA catalytic subunits. Patients with PRKAR1A mutations are often diagnosed with Carney complex (CNC) in early adulthood, and may die later in life from cardiac complications such as heart failure. However, it remains unknown whether PRKAR1A deficiency interferes with normal heart development...
March 2020: Physiological Reports
https://read.qxmd.com/read/32212196/sirt3-is-dispensable-for-oocyte-quality-and-female-fertility-in-lean-and-obese-mice
#3
Juvita D Iljas, Hayden A Homer
Mammalian oocytes rely heavily on mitochondrial oxidative phosphorylation (OXPHOS) for generating ATP. However, mitochondria are also the primary source of damaging reactive oxygen species (ROS). Mitochondrial de-regulation, therefore, underpins poor oocyte quality associated with conditions such as obesity and aging. The mitochondrial sirtuin, Sirt3, is critical for mitochondrial respiration and redox regulation. Interestingly, however, Sirt3 knockout (Sirt3-/- ) mice do not exhibit systemic compromise under basal conditions, only doing so under stressed conditions such as high-fat diet (HFD)-induced obesity...
March 24, 2020: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/32211858/fxr1-is-a-novel-mre11-binding-partner-and-participates-in-oxidative-stress-responses
#4
Fei Qi, Qingmei Meng, Ikue Hayashi, Junya Kobayashi
Ataxia-telangiectasia (AT) and MRE11-defective Ataxia-telangiectasia-like disorder (ATLD) patients show progressive cerebellar ataxia. ATM, mutated in AT, can be activated in response to oxidative stress as well as DNA damage, which could be linked to disease-related neurodegeneration. However, the role of MRE11 in oxidative stress responses has been elusive. Here, we showed that MRE11 could participate in ATM activation during oxidative stress in an NBS1/RAD50-independent manner. Importantly, MRE11 was indispensable for ATM activation...
March 25, 2020: Journal of Radiation Research
https://read.qxmd.com/read/32211148/high-throughput-proteomics-fiber-typing-profit-for-comprehensive-characterization-of-single-skeletal-muscle-fibers
#5
Sebastian Kallabis, Lena Abraham, Stefan Müller, Verena Dzialas, Clara Türk, Janica Lea Wiederstein, Theresa Bock, Hendrik Nolte, Leonardo Nogara, Bert Blaauw, Thomas Braun, Marcus Krüger
Background: Skeletal muscles are composed of a heterogeneous collection of fiber types with different physiological adaption in response to a stimulus and disease-related conditions. Each fiber has a specific molecular expression of myosin heavy chain molecules (MyHC). So far, MyHCs are currently the best marker proteins for characterization of individual fiber types, and several proteome profiling studies have helped to dissect the molecular signature of whole muscles and individual fibers...
2020: Skeletal Muscle
https://read.qxmd.com/read/32206162/proteomic-identification-of-allergenic-proteins-in-red-oak-quercus-rubra-pollen
#6
José Ángel Huerta-Ocampo, Alejandra Valenzuela-Corral, María Del Refugio Robles-Burgueño, Ana María Guzmán-Partida, Miguel Ángel Hernández-Oñate, Luz Vázquez-Moreno, Gandhi F Pavón-Romero, Luis M Terán
Background: Red oak pollen is an important cause of allergic respiratory disease and it is widely distributed in North America and central Europe. To date, however, red oak pollen allergens have not been identified. Here, we describe the allergenic protein profile from red oak pollen. Methods: Total proteins were extracted from red oak pollen using a modified phenolic extraction method, and, subsequently, proteins were separated by two-dimensional gel electrophoresis (2DE) for both total protein stain (Coomassie Blue) and immunoblotting...
March 2020: World Allergy Organization Journal
https://read.qxmd.com/read/32201887/greater-skeletal-muscle-oxidative-capacity-is-associated-with-higher-resting-metabolic-rate-results-from-the-baltimore-longitudinal-study-of-aging
#7
Marta Zampino, Richard D Semba, Fatemeh Adelnia, Richard G Spencer, Kenneth W Fishbein, Jennifer A Schrack, Eleanor M Simonsick, Luigi Ferrucci
BACKGROUND: Resting metabolic rate (RMR) tends to decline with aging. The age-trajectory of decline in RMR is similar to changes that occur in muscle mass, muscle strength and fitness but while the decline in these phenotypes have been related to changes of mitochondrial function and oxidative capacity, whether lower RMR is associated with poorer mitochondrial oxidative capacity is unknown. METHODS: In 619 participants of the Baltimore Longitudinal Study of Aging, we analyzed the cross-sectional association between RMR (kcal/day), assessed by indirect calorimetry, and skeletal muscle maximal oxidative phosphorylation capacity, assessed as post-exercise phosphocreatine recovery time constant (τPCr), by phosphorous magnetic resonance spectroscopy...
March 23, 2020: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://read.qxmd.com/read/32201093/m-csf-and-l929-derived-macrophages-present-distinct-metabolic-profiles-with-similar-inflammatory-outcomes
#8
Lauar de Brito Monteiro, Gustavo Gastão Davanzo, Cristhiane Fávero de Aguiar, Felipe Corrêa da Silva, Jessica Rodrigues de Andrade, Ana Campos Codo, Jessica Aparecida da Silva Pereira, Leonardo Pimentel de Freitas, Pedro Manoel Moraes-Vieira
Macrophages are essential components of the immune system. Macrophages can be derived from the bone marrow of mice with either recombinant M-CSF or L929 supernatant. Recent literature considers recombinant M-CSF- and L929-derived macrophages as equals, even though L929-derived macrophages are exposed to other substances secreted in the L929 supernatant, and not only M-CSF. Thus, we decided to perform a comparative analysis of both inflammatory and metabolic profiles of macrophages differentiated under the aforementioned conditions, which is relevant for standardization and interpretation of in vitro studies...
March 19, 2020: Immunobiology
https://read.qxmd.com/read/32197068/glycolysis-independent-glucose-metabolism-distinguishes-te-from-icm-fate-during-mammalian-embryogenesis
#9
Fangtao Chi, Mark S Sharpley, Raghavendra Nagaraj, Shubhendu Sen Roy, Utpal Banerjee
The mouse embryo undergoes compaction at the 8-cell stage, and its transition to 16 cells generates polarity such that the outer apical cells are trophectoderm (TE) precursors and the inner cell mass (ICM) gives rise to the embryo. Here, we report that this first cell fate specification event is controlled by glucose. Glucose does not fuel mitochondrial ATP generation, and glycolysis is dispensable for blastocyst formation. Furthermore, glucose does not help synthesize amino acids, fatty acids, and nucleobases...
March 12, 2020: Developmental Cell
https://read.qxmd.com/read/32195285/comparative-proteomic-analysis-of-two-manilkara-species-leaves-under-nacl-stress
#10
Yumei Liu, Chongling Yan, Zhiyu Song, Shuang Zhou
Background: Salinity is a major environmental limiting factor, which affect agricultural production. The two Manilkara seedlings ( M. roxburghiana and M. zapota ) with high economic importance, could not adapt well to higher soil salinity and little is known about their proteomic mechanisms. Objectives: The mechanisms responsible for the effects of salinity on the two Manilkara species leaves were examined by means of proteomic analysis. Material and Methods: The seedlings were cultivated in a greenhouse and treated with NaCl...
September 2019: Iranian Journal of Biotechnology
https://read.qxmd.com/read/32195257/exploring-the-hela-dark-mitochondrial-proteome
#11
Federica Marini, Victor Corasolla Carregari, Viviana Greco, Maurizio Ronci, Federica Iavarone, Silvia Persichilli, Massimo Castagnola, Andrea Urbani, Luisa Pieroni
In the framework of the Human Proteome Project initiative, we aim to improve mapping and characterization of mitochondrial proteome. In this work we implemented an experimental workflow, combining classical biochemical enrichments and mass spectrometry, to pursue a much deeper definition of mitochondrial proteome and possibly mine mitochondrial uncharacterized dark proteins . We fractionated in two compartments mitochondria enriched from HeLa cells in order to annotate 4230 proteins in both fraction by means of a multiple-enzyme digestion (trypsin, chymotrypsin and Glu-C) followed by mass spectrometry analysis using a combination of Data Dependent Acquisition (DDA) and Data Independent Acquisition (DIA)...
2020: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/32191449/synthesis-characterization-cytotoxic-activity-and-metabolic-studies-of-ruthenium-ii-polypyridyl-complexes-containing-flavonoid-ligands
#12
Alexandra-Cristina Munteanu, Anna Notaro, Marta Jakubaszek, Joseph Cowell, Mickaël Tharaud, Bruno Goud, Valentina Uivarosi, Gilles Gasser
Four novel monocationic Ru(II) polypyridyl complexes were synthesized with the general formula [Ru(DIP)2 flv]X, where DIP is 4,7-diphenyl-1,10-phenanthroline, flv stands for the flavonoid ligand (5-hydroxyflavone in [Ru(DIP)2 (5-OHF)](PF6 ), genistein in [Ru(DIP)2 (gen)](PF6 ), chrysin in [Ru(DIP)2 (chr)](OTf), and morin in [Ru(DIP)2 (mor)](OTf)), and X is the counterion, PF6 - , and OTf ̅ (triflate, CF3 SO3 ̅), respectively. Following the chemical characterization of the complexes by 1 H and 13 C NMR, mass spectrometry, and elemental analysis, their cytotoxicity was tested against several cancer cell lines...
March 19, 2020: Inorganic Chemistry
https://read.qxmd.com/read/32189351/association-of-body-composition-physical-functioning-and-protein-intake-in-adult-patients-with-mitochondrial-diseases
#13
Heidi E E Zweers, Valentine Bordier, Jeanne In 't Hulst, Mirian C H Janssen, Geert J A Wanten, Susanne Leij-Halfwerk
BACKGROUND: Whether decreased physical functioning of patients with mitochondrial disease (MD) is related to altered body composition or low protein intake needs clarification at the background of the nutrition state. METHODS: In this 2-site cross-sectional study, MD patients were age-, body mass index (BMI)-, and gender-matched to controls. Body composition was assessed by dual-energy x-ray absorptiometry. Physical functioning was measured by handgrip strength, 6-minute walking test, 30-second sit-to-stand test (30SCT), and 6-minute mastication test...
March 19, 2020: JPEN. Journal of Parenteral and Enteral Nutrition
https://read.qxmd.com/read/32188147/proanthocyanidins-ameliorated-deficits-of-lipid-metabolism-in-type-2-diabetes-mellitus-via-inhibiting-adipogenesis-and-improving-mitochondrial-function
#14
Fangfang Tie, Jifei Wang, Yuexin Liang, Shujun Zhu, Zhenhua Wang, Gang Li, Honglun Wang
Proanthocyanidins are the major active compounds extracted from Iris lactea Pall. var. Chinensis (Fisch.) Koidz (I. lactea ). Proanthocyanidins exhibit a variety of pharmacological activities such as anti-oxidation, anti-inflammation, anti-tumor, and lowering blood lipids. However, the underlying mechanism of its regulating effect on lipid metabolism in diabetic conditions remains unclear. The present study investigated the effects of I . lactea -derived proanthocyanidins on lipid metabolism in mice of type 2 diabetes mellitus (T2DM)...
March 16, 2020: International Journal of Molecular Sciences
https://read.qxmd.com/read/32184719/the-effect-of-silibinin-on-protein-expression-profile-in-white-adipose-tissue-of-obese-mice
#15
Fei Wang, Shuchun Chen, Luping Ren, Yichao Wang, Zelin Li, Tiantian Song, He Zhang, Qiwen Yang
Objective: To investigate the effect of silibinin on the protein expression profile of white adipose tissue (WAT) in obese mice by using Tandem Mass Tag (TMT) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods: According to experimental requirements, 36 C57BL/6JC mice were randomly divided into normal diet group (WC group), high fat diet group (WF group), and high fat diet + silibinin group (WS group). WS group was intragastrically administered with 54 mg/kg body weight of silibinin, and the WC group and the WF group were intragastrically administered with equal volume of normal saline...
2020: Frontiers in Pharmacology
https://read.qxmd.com/read/32184324/absolute-yeast-mitochondrial-proteome-quantification-reveals-trade-off-between-biosynthesis-and-energy-generation-during-diauxic-shift
#16
Francesca Di Bartolomeo, Carl Malina, Kate Campbell, Maurizio Mormino, Johannes Fuchs, Egor Vorontsov, Claes M Gustafsson, Jens Nielsen
Saccharomyces cerevisiae constitutes a popular eukaryal model for research on mitochondrial physiology. Being Crabtree-positive, this yeast has evolved the ability to ferment glucose to ethanol and respire ethanol once glucose is consumed. Its transition phase from fermentative to respiratory metabolism, known as the diauxic shift, is reflected by dramatic rearrangements of mitochondrial function and structure. To date, the metabolic adaptations that occur during the diauxic shift have not been fully characterized at the organelle level...
March 17, 2020: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/32183792/evaluation-of-antimicrobial-activity-of-the-hydrolate-of-coridothymus-capitatus-l-reichenb-fil-lamiaceae-alone-and-in-combination-with-antimicrobial-agents
#17
Andreana Marino, Antonia Nostro, Narcisa Mandras, Janira Roana, Giovanna Ginestra, Natalizia Miceli, Maria Fernanda Taviano, Fabrizio Gelmini, Giangiacomo Beretta, Vivian Tullio
BACKGROUND: Hydrolates, complex mixtures containing traces of essential oils (EOs), are inexpensive, easy to make and less toxic than their corresponding EOs. The antibacterial and antifungal activity of the hydrolate of Coridothymus capitatus (L.) Reichenb. fil. (Lamiaceae) alone and in combination with antimicrobial drugs, such as tetracycline and itraconazole, were evaluated. METHODS: The chemical composition was analysed by gas-chromatography-mass spectrometry (GC-MS)...
March 17, 2020: BMC complementary medicine and therapies
https://read.qxmd.com/read/32183350/maternal-fat-1-transgene-protects-offspring-from-excess-weight-gain-oxidative-stress-and-reduced-fatty-acid-oxidation-in-response-to-high-fat-diet
#18
Kristen E Boyle, Margaret J Magill-Collins, Sean A Newsom, Rachel C Janssen, Jacob E Friedman
Overweight and obesity accompanies up to 70% of pregnancies and is a strong risk factor for offspring metabolic disease. Maternal obesity-associated inflammation and lipid profile are hypothesized as important contributors to excess offspring liver and skeletal muscle lipid deposition and oxidative stress. Here, we tested whether dams expressing the fat-1 transgene, which endogenously converts omega-6 (n-6) to omega-3 (n-3) polyunsaturated fatty acid, could protect wild-type (WT) offspring against high-fat diet induced weight gain, oxidative stress, and disrupted mitochondrial fatty acid oxidation...
March 14, 2020: Nutrients
https://read.qxmd.com/read/32183169/quantification-of-plasma-and-urine-thymidine-and-2-deoxyuridine-by-lc-ms-ms-for-the-pharmacodynamic-evaluation-of-erythrocyte-encapsulated-thymidine-phosphorylase-in-patients-with-mitochondrial-neurogastrointestinal-encephalomyopathy
#19
Karin Kipper, Max Hecht, Natalicia J Antunes, Lynette D Fairbanks, Michelle Levene, Sema Kalkan Uçar, Andrew Schaefer, Emma L Blakely, Bridget E Bax
Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is an ultra-rare disorder caused by mutations in TYMP , leading to a deficiency in thymidine phosphorylase and a subsequent systemic accumulation of thymidine and 2'-deoxyuridine. Erythrocyte-encapsulated thymidine phosphorylase (EE-TP) is under clinical development as an enzyme replacement therapy for MNGIE. Bioanalytical methods were developed according to regulatory guidelines for the quantification of thymidine and 2'-deoxyuridine in plasma and urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for supporting the pharmacodynamic evaluation of EE-TP...
March 13, 2020: Journal of Clinical Medicine
https://read.qxmd.com/read/32181106/-schinus-terebinthifolia-leaf-lectin-stell-is-an-immunomodulatory-agent-by-altering-cytokine-release-by-mice-splenocytes
#20
Ardilles Juan Carlos Alves Dos Santos, Bárbara Rafaela da Silva Barros, Lethícia Maria de Souza Aguiar, Leydianne Leite de Siqueira Patriota, Thâmarah de Albuquerque Lima, Russolina Benedeta Zingali, Patrícia Maria Guedes Paiva, Thiago Henrique Napoleão, Cristiane Moutinho Lagos de Melo, Emmanuel Viana Pontual
Schinus terebinthifolia leaf lectin (SteLL) was reported to be an antimicrobial and antitumor agent. In this work, we evaluated the immunomodulatory activity of SteLL on mice splenocytes and also determined its native molecular mass and putative sequence similarities with plant proteins. The effects of SteLL (12.5 μg/mL) on viability, cytosolic Ca2+ concentration ([Ca2+ ]cyt ), cytosolic and mitochondrial levels of reactive oxygen species (ROS), and mitochondrial transmembrane potential (ΔΨm) of mice splenocytes were determined...
April 2020: 3 Biotech
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