Anshu Saran, Hey-Min Kim, Ireland Manning, Mark A Hancock, Claus Schmitz, Mariusz Madej, Jan Potempa, Maria Sola, Jean-François Trempe, Yongtao Zhu, Mary Ellen Davey, Natalie Zeytuni
The Type-IX secretion system (T9SS) is a nanomachinery utilized by bacterial pathogens to facilitate infection. The system is regulated by a signaling cascade serving as its activation switch. A pivotal member in this cascade, the response regulator protein PorX, represents a promising drug target to prevent the secretion of virulence factors. Here, we provide a comprehensive characterization of PorX both in vitro and in vivo . First, our structural studies revealed PorX harbours a unique enzymatic effector domain, which, surprisingly, shares structural similarities with the alkaline phosphatase superfamily, involved in nucleotide and lipid signaling pathways...
May 15, 2024: bioRxiv