Rihao Qu, Xiuyuan Cheng, Esen Sefik, Jay S Stanley Iii, Boris Landa, Francesco Strino, Sarah Platt, James Garritano, Ian D Odell, Ronald Coifman, Richard A Flavell, Peggy Myung, Yuval Kluger
Single-cell RNA sequencing has been widely used to investigate cell state transitions and gene dynamics of biological processes. Current strategies to infer the sequential dynamics of genes in a process typically rely on constructing cell pseudotime through cell trajectory inference. However, the presence of concurrent gene processes in the same group of cells and technical noise can obscure the true progression of the processes studied. To address this challenge, we present GeneTrajectory, an approach that identifies trajectories of genes rather than trajectories of cells...
April 5, 2024: Nature Biotechnology