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Colton blood group system

Sofia L Crottet
This article reviews information regarding the clinical significance of antibodies to antigens in the Scianna, Dombrock, Colton, Landsteiner-Wiener, Chido/Rodgers, H, Kx, Cromer, Gerbich, Knops, Indian, and Ok blood group systems. Like most blood group systems, antibodies to many of the antigens in these groups are rarely encountered because of the high prevalence of the associated antigens in most populations. For many, the clinical significance-that is, the potential to cause reduced survival of transfused antigen-positive red blood cells or a transfusion reaction (e...
September 2018: Immunohematology
Franz F Wagner, Andrea Doescher, Rita Bittner, Thomas H Müller
Background: PCR with sequence-specific priming using allele-specific fluorescent primers and analysis on a capillary sequencer is a standard technique for DNA typing. We aimed to adapt this method for donor typing in a medium-throughput setting. Methods: Using the Extract-N-Amp PCR system, we devised a set of eight multiplex allele-specific PCR with fluorescent primers for Fya /Fyb , Jka /Jkb , M/N, and S/s. The alleles of a gene were discriminated by the fluorescent color; donor and polymorphism investigated were encoded by product length...
July 2018: Transfusion Medicine and Hemotherapy
Avani Shah, Keyuri Jariwala, Snehalata Gupte, Preeti Sharma, Kanchan Mishra, Kanjaksha Ghosh
BACKGROUND: Extended phenotyping is one of the important method of reducing red cell alloimmunisation. Extended phenotyping of red cells from voluntary donors have many uses in addition to its application in population genetics. As there was very little data extended phenotyping on a cohort of Indian Voluntary blood donors this project was undertaken. STUDY DESIGN & METHODOLOGY: 200 regular voluntary blood donors having 'O' blood group were included for red cell antigen typing of Rh (D,C,E,c,e), Kell (K, k, Kpa , Kpb ), Duffy (Fya , Fyb ), Kidd (Jka , Jkb ), Lewis(Lea , Leb ), P(P1), MNS (M, N,S,s), and Lutheran (Lua , Lub ), Colton (Coa , Cob ), Diago (Diaa , Wra ), Vw and Xga antigens using conventional antisera provided by DIAGAST...
September 5, 2018: Transfusion and Apheresis Science
Stéphanie A Boccoz, Julien Fouret, Magali Roche, Joël Lachuer, Catherine Legras-Lachuer, Benjamin P Corgier, Christophe A Marquette
OBJECTIVES: Thirty-six blood group systems are listed by the International Society of Blood Transfusion, containing almost 350 antigens. Most of these result from a single nucleotide polymorphism (SNP). Serology is the standard method for blood group typing. However, this technique has some limitations and cannot respond to the growing demand of blood product typing for a large number of antigens. Here we describe a blood group genotyping assay directly from whole blood samples using Next-Generation Sequencing (NGS), allowing the simultaneous identification of 15 SNPs associated with the blood group systems of 95 patients in a single run...
September 2018: Clinical Biochemistry
Yun Ji Hong, Yousun Chung, Sang Mee Hwang, Jeong Su Park, Jeong-Ran Kwon, Young Sill Choi, Jun Nyun Kim, Dong Han Lee, So-Yong Kwon, Nam-Sun Cho, Eun Young Song, Kyoung Un Park, Junghan Song, Kyou Sup Han
It is often difficult for standard blood banks in Korea to supply adequate amounts of blood for patients with rare phenotype. Moreover, the definition of a blood in need is ambiguous, and much remains to be learned. In this study, we determined the prevalence of various red blood cell (RBC) antigens from a donor viewpoint and estimated the demand for specific antigen-negative blood from a patient viewpoint. Our data will aid the establishment of a Rare Blood Program in Korea (KRBP). RBC genotyping of 419 blood donors was performed using a Lifecodes RBC/RBC-R typing kit (Immucor, Norcross, GA)...
May 2016: Annals of Hematology
Yan Chen, Ling Ma, Yan-Chun Liu
OBJECTIVE: To investigate the distribution of Colton, Diego, Kell and Yt rare blood groups in Chinese Nanjing Han population, so as to improve the transfusion capability of patients with rare blood group and to further enrich the rare-blood-donor bank. METHODS: A total of 2 015 blood samples from the blood donors were selected randomly to screen the presence of K⁺ and Kp(c+) (Kell), Yt(b+) (Yt), Co(b+) (Colton), Di(a+b+) and Di(a+b-) (Digeo) antigen allele by using PCR and multiplex PCR...
October 2015: Zhongguo Shi Yan Xue Ye Xue za Zhi
Angela Belsito, Dario Costa, Carmela Fiorito, Gustavo De Iorio, Amelia Casamassimi, Silverio Perrotta, Claudio Napoli
BACKGROUND AND OBJECTIVES: Although minor erythrocyte antigens are not considered clinically significant in sporadic transfusions, they may be relevant for multi-transfusion patients. When serological assay is not conceivable, molecular genotyping allows predicting the red blood cell phenotype, extending the typing until minor blood groups. The aim of this study was to evaluate the utility of blood group genotyping and compare the molecular typing of erythrocyte antigens with the established serological methods...
February 2015: Transfusion and Apheresis Science
S Vege, S Nance, D Kavitsky, X Li, T Horn, G Meny, C M Westhoff
The Colton (CO) blood group system consists of four antigens, Co(a), Co(b), Co3, and Co4, located on aquaporin-1 (AQP1), with Co(a) highly prevalent in all populations (99.8%). The Colton null phenotype, Co(a-b-), is very rare, and individuals with this phenotype lack the high-prevalence antigen Co3. To date, only six Co(a-b-) probands have been reported and four silencing alleles characterized. We identified an AQP1-null allele in a white woman with anti-Co3 caused by deletion of a G at nucleotide 601 (nt601delG) that results in a frameshift and premature termination (Val201Stop)...
2013: Immunohematology
S Mazières, S A Temory, H Vasseur, P Gallian, J Di Cristofaro, J Chiaroni
BACKGROUND AND OBJECTIVES: Blood incompatibility arises from individual and ethnic differences in red blood cell (RBC) antigen profiles. This underlines the importance of documenting RBC antigen variability in various ethnic groups. Central Asia is an area with a long and complex migratory history. The purpose of this article is to describe key antigen frequencies of Afghan ethnic groups in the Hindu-Kush region of Afghanistan as a basis for improving blood transfusion practices in that area...
June 2013: Transfusion Medicine
L Colton, M Kosoy
To date no experimental infection studies have been conducted in laboratory mice using Mus spp. bartonella strains. Therefore we designed a study to evaluate the in vivo infection characteristics of 2 Bartonella tribocorum strains from wild Mus spp. in laboratory mice with the aim of developing a mouse model that reproduces characteristics of naturally acquired bartonella infections in rodents. Groups of outbred CD1 female mice were subcutaneously inoculated with low doses of 2 mouse bartonella strains (10, 100, and 1000 bacteria/mouse)...
January 2013: Parasitology
Barbara Ludwig, Avi Rotem, Janine Schmid, Gordon C Weir, Clark K Colton, Mathias D Brendel, Tova Neufeld, Norman L Block, Karina Yavriyants, Anja Steffen, Stefan Ludwig, Triantafyllos Chavakis, Andreas Reichel, Dimitri Azarov, Baruch Zimermann, Shiri Maimon, Mariya Balyura, Tania Rozenshtein, Noa Shabtay, Pnina Vardi, Konstantin Bloch, Paul de Vos, Andrew V Schally, Stefan R Bornstein, Uriel Barkai
Islet transplantation is a feasible therapeutic alternative for metabolically labile patients with type 1 diabetes. The primary therapeutic target is stable glycemic control and prevention of complications associated with diabetes by reconstitution of endogenous insulin secretion. However, critical shortage of donor organs, gradual loss in graft function over time, and chronic need for immunosuppression limit the indication for islet transplantation to a small group of patients. Here we present a promising approach to address these limitations by utilization of a macrochamber specially engineered for islet transplantation...
March 27, 2012: Proceedings of the National Academy of Sciences of the United States of America
Erwin Strobel
BACKGROUND: The first monoclonal anti-k (anti-KEL2) reagent of the IgM-class (clone: LK1) for the tube spin method is now commercially available. As this reagent is directly agglutinating in contrast to conventional polyclonal anti-k reagents requiring an indirect antiglobulin test, we studied the reaction characteristics of this new reagent carefully before starting its use in our routine blood grouping laboratory. METHODS: The titer of the monoclonal anti-k reagent (manufacturer: Biotest, D-63303 Dreieich) was compared with that of two polyclonal anti-k reagents...
2010: Clinical Laboratory
G R Halverson, T Peyrard
No abstract text is available yet for this article.
2010: Immunohematology
Lionel Arnaud, Virginie Helias, Cécile Menanteau, Thierry Peyrard, Nicole Lucien, Pierre Ripoche, Régine Lapègue, Bach-Nga Pham, Pierre-Yves Le Pennec, John J Moulds, Jean-Pierre Cartron
BACKGROUND: The Colton blood group system currently comprises three antigens, Co(a) , Co(b) , and Co3. The latter is only absent in the extremely rare individuals of the Colton "null" phenotype, usually referred to as Co(a-b-), which lack the water channel AQP1 that carries the Colton antigens. The discovery of a Co(a-b-) individual with no AQP1 deficiency suggested another molecular basis for the Co(a-b-) phenotype. STUDY DESIGN AND METHODS: Red blood cells were analyzed by stopped-flow light scattering and Western blotting and typed by hemagglutination and flow cytometry...
October 2010: Transfusion
M H Tian, G R Halverson
Anti-Ok(a) was first described by Morel and Hamilton in 1979. The Ok(a) antigen has a very high incidence, and only eight probands that are Ok(a-) have been found; all are of Japanese heritage. In this study,we describe the generation and characterization of three novel monoclonal antibodies (Mabs), MIMA-25, MIMA-144, and MIMA-149. The reactivity of these three Mabs was compared with the original human polyclonal anti-Ok(a). Mice were immunized with transfected HEK cells to induce an immune response, and the spleen B lymphocytes were fused with mouse myeloma X63-Ag8...
2009: Immunohematology
K Karpasitou, S Frison, E Longhi, F Drago, R Lopa, F Truglio, M Marini, S Bresciani, M Scalamogna, F Poli
BACKGROUND: The antigens of the Colton blood group system, Co(a) and Co(b), are encoded by a single gene that produces the aquaporin-1 (AQP1) protein, a water channel-forming protein, and are characterized by a single nucleotide polymorphism (SNP). A healthy Caucasoid blood donor originally typed as Co(a-b-) with commercial anti-Co(b) typed Co(a-b+) when retested with another anti-Co(b). Retyped with two different molecular biology methods, the sample came out Co(a)/Co(b). With the aim of understanding these discrepancies, serological, cytometric and molecular biology tests were carried out...
August 1, 2010: Vox Sanguinis
K R Ribeiro, M H Guarnieri, D C da Costa, F F Costa, J Pellegrino, L Castilho
BACKGROUND: Blood samples from patients with sickle cell disease (SCD) present to transfusion service with numerous antibodies, making the searching for compatible red blood cells (RBC) a challenge. To overcome this problem we developed an effective strategy to meet needs of supplying RBC-compatible units to SCD patients using DNA arrays. METHODS: We selected DNA samples from 144 SCD patients with multiple (receiving > 5 units) transfusions previously phenotyped for ABO, Rh(D, C, c, E, e), K1, Fy(a) and Jk(a)...
August 2009: Vox Sanguinis
Mhammed Touinssi, Jacques Chiaroni, Anna Degioanni, Thomas Granier, Olivier Dutour, Pascal Bailly, Frédéric Bauduer
The Basques demonstrate peculiar characteristics regarding blood group systems. Although ABO, Rhesus, and Duffy have been extensively studied in this population, the distribution of other groups remains largely unknown. Therefore, we evaluated the frequency of less-explored- or still noninvestigated blood groups using DNA-based assays and interpreted these data in the view of population genetics. Polymorphisms of KEL (Kell), SLCA14A1 (Kidd), GYPA/GYPB (MNS), ART4 (Dombrock), AQP1 (Colton), and ACHE (Yt) blood group genes were determined from a sample of more than 100 autochthonous French Basques using allele-specific primer PCR (PCR-ASP) methods...
May 2008: American Journal of Human Biology: the Official Journal of the Human Biology Council
Markus T Rojewski, Hubert Schrezenmeier, Willy A Flegel
The proteins of blood group systems are expressed on red blood cells (RBC) by definition. We searched nucleotide databases of human expressed sequence tags (EST) to collate the distribution of 22 distinct membrane proteins in cells and tissues other than RBC. The documented blood group genes are: MNS, Rh, Lutheran, Kell, Duffy, Kidd, Diego, Yt, Xg, Scianna, Dombrock, Colton, Landsteiner-Wiener, Kx, Gerbich, Cromer, Knops, Indian, Ok, Raph, John-Milton-Hagen and Gill. The genes were grouped according to their overall and their relative expression in embryo and adults...
August 2006: Transfusion and Apheresis Science
Ralph Ballerstadt, Colton Evans, Ashok Gowda, Roger McNichols
The in vivo performance of a transdermal near-infrared fluorescence resonance energy transfer (FRET) affinity sensor was investigated in hairless rats, in order to validate its feasibility for glucose monitoring in humans. The sensor itself consists of a small hollow fiber implanted in dermal skin tissue, containing glucose-sensitive assay chemistry composed of agarose-immobilized Concanavalin A (ConA) and free dextran. The glucose-dependent fluorescence change is based on FRET between near-infrared-compatible donor and quencher dyes that are chemically linked to dextran and ConA, respectively...
June 2006: Diabetes Technology & Therapeutics
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