keyword
https://read.qxmd.com/read/38518507/chemoorganotrophic-electrofermentation-by-cupriavidus-necator-using-redox-mediators
#1
JOURNAL ARTICLE
André Gemünde, Elena Rossini, Oliver Lenz, Stefan Frielingsdorf, Dirk Holtmann
The non-pathogenic β-proteobacterium Cupriavidus necator has the ability to switch between chemoorganotrophic, chemolithoautotrophic and electrotrophic growth modes, making this microorganism a widely used host for cellular bioprocesses. Oxygen usually acts as the terminal electron acceptor in all growth modes. However, several challenges are associated with aeration, such as foam formation, oxygen supply costs, and the formation of an explosive gas mixture in chemolithoautotrophic cultivation with H2 , CO2 and O2 ...
March 18, 2024: Bioelectrochemistry
https://read.qxmd.com/read/38513684/safety-and-immunogenicity-of-the-co-administered-na-apr-1-and-na-gst-1-hookworm-vaccines-in-school-aged-children-in-gabon-a-randomised-controlled-observer-blind-phase-1-dose-escalation-trial
#2
JOURNAL ARTICLE
Jeannot F Zinsou, David J Diemert, Jean Claude Dejon-Agobé, Bayodé R Adégbité, Yabo Josiane Honkpehedji, Kafui G Vodonou, Rodrigue Bikangui, Jean Ronald Edoa, Marguerite Massinga Loembe, Guangzhao Li, Maria Yazdanbakhsh, Maria Elena Bottazzi, Remko van Leeuwen, Peter G Kremsner, Peter J Hotez, Jeffrey M Bethony, Martin P Grobusch, Ayola A Adegnika
BACKGROUND: A human hookworm vaccine is being developed to protect children against iron deficiency and anaemia associated with chronic infection with hookworms. Necator americanus aspartic protease-1 (Na-APR-1) and N americanus glutathione S-transferase-1 (Na-GST-1) are components of the blood digestion pathway critical to hookworm survival in the host. Recombinant Na-GST-1 and catalytically inactive Na-APR-1 (Na-APR-1[M74]) adsorbed to Alhydrogel were safe and immunogenic when delivered separately or co-administered to adults in phase 1 trials in non-endemic and endemic areas...
March 18, 2024: Lancet Infectious Diseases
https://read.qxmd.com/read/38507576/chimeric-mutations-in-grapevine-enhanced-disease-resistance1-improve-resistance-to-powdery-mildew-without-growth-penalty
#3
JOURNAL ARTICLE
Xue-Na Yu, Ye Guo, Qianling Yang, Haiyan Yu, Meng-Jiao Lu, Liang Zhao, Zhuo-Shuai Jin, Xiang-Nan Xu, Jia-Yue Feng, Ying-Qiang Wen
Grapevine (Vitis vinifera L.) incurs severequality degradation and yield loss from powdery mildew, a major fungal disease caused by Erysiphe necator. ENHANCED DISEASE RESISTANCE1 (EDR1), a Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK), negatively regulates defense responses against powdery mildew in Arabidopsis (Arabidopsis thaliana). However, little is known about the role of the putatively orthologous EDR1 gene in grapevine. In this study, we obtained grapevine VviEDR1-edited lines using CRISPR/Cas9...
March 20, 2024: Plant Physiology
https://read.qxmd.com/read/38460564/biological-carbon-capture-from-biogas-streams-insights-into-cupriavidus-necator-autotrophic-growth-and-transcriptional-profile
#4
JOURNAL ARTICLE
Rebecca Serna-García, Maria Silvia Morlino, Luca Bucci, Filippo Savio, Lorenzo Favaro, Tomas Morosinotto, Aurora Seco, Alberto Bouzas, Stefano Campanaro, Laura Treu
Recycling carbon-rich wastes into high-value platform chemicals through biological processes provides a sustainable alternative to petrochemicals. Cupriavidus necator, known for converting carbon dioxide (CO2 ) into polyhydroxyalkanoates (PHA) was studied for the first time using biogas streams as the sole carbon source. The bacterium efficiently consumed biogenic CO2 from raw biogas with methane at high concentrations (50%) proving non-toxic. Continuous addition of H2 and O2 enabled growth trends comparable to glucose-based heterotrophic growth...
March 7, 2024: Bioresource Technology
https://read.qxmd.com/read/38452952/heterotrophic-and-autotrophic-production-of-l-isoleucine-and-l-valine-by-engineered-cupriavidus-necator-h16
#5
JOURNAL ARTICLE
Lei Wang, Junhu Yao, Tao Tu, Bin Yao, Jie Zhang
Advancement in commodity chemical production from carbon dioxide (CO2 ) offers a promising path towards sustainable development goal. Cupriavidus necator is an ideal host to convert CO2 into high-value chemicals, thereby achieving this target. Here, C. necator was engineered for heterotrophic and autotrophic production of L-isoleucine and L-valine. Citramalate synthase was introduced to simplify isoleucine synthesis pathway. Blocking poly-hydroxybutyrate biosynthesis resulted in significant accumulation of isoleucine and valine...
March 5, 2024: Bioresource Technology
https://read.qxmd.com/read/38441714/management-of-paediatric-soil-transmitted-helminthiasis-in-a-non-endemic-area-experience-in-a-reference-international-health-unit
#6
JOURNAL ARTICLE
Maria Espiau, Sara Ajanovic, Francesc Zarzuela, Carles Rubio Maturana, Pere Soler-Palacín, Antoni Soriano-Arandes, Elena Sulleiro
Soil-transmitted helminth (STH) infections inflict disability worldwide, especially in the poorest communities. Current therapeutic options against STHs show limited efficacy, particularly against Trichuris trichiura. The empirical management of patients coming from high-prevalence areas has been suggested for non-endemic areas. This study aimed to describe the management of STH infections in a non-endemic setting using an individualised approach. We performed a retrospective, descriptive study of all patients up to 16 years of age with STH infections attended at an international health unit in a non-endemic area (2014-2018), including all T...
March 5, 2024: Parasitology Research
https://read.qxmd.com/read/38440767/intestinal-parasites-associated-with-american-cockroach-periplaneta-americana-in-akure-ondo-state-nigeria
#7
JOURNAL ARTICLE
Adebayo Victor Akeju, Titus Adeniyi Olusi, Regina-Tyra Ekwi Obi
American cockroach ( Periplaneta americana ) has been implicated as mechanical vector of parasites of humans and animals. Therefore, this study aimed to identify and determine the prevalence of human intestinal parasites associated with the body surface and gut of P. americana . A total of 221 cockroaches which include 104 males and 117 females were collected from household kitchen, toilet area and canteen after which they were brought to laboratory for study. The body surface of the cockroach was washed with 5 ml normal saline solution to remove external parasites on the body surface for examination and later rinsed with 70% alcohol and dried before dissecting...
March 2024: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://read.qxmd.com/read/38387639/tailored-polyhydroxyalkanoate-production-from-renewable-non-fatty-acid-carbon-sources-using-engineered-cupriavidus-necator-h16
#8
JOURNAL ARTICLE
Soyoung Park, Soonjong Roh, Jin Yoo, Jung Ho Ahn, Gyeongtaek Gong, Sun-Mi Lee, Youngsoon Um, Sung Ok Han, Ja Kyong Ko
As thermoplastic, nontoxic, and biocompatible polyesters, polyhydroxyalkanoates (PHAs) are considered promising biodegradable plastic candidates for diverse applications. Short-chain-length/medium-chain-length (SCL/MCL) PHA copolymers are flexible and versatile PHAs that are typically produced from fatty acids, which are expensive and toxic. Therefore, to achieve the sustainable biosynthesis of SCL/MCL-PHAs from renewable non-fatty acid carbon sources (e.g., sugar or CO2 ), we used the lithoautotrophic bacterium Cupriavidus necator H16 as a microbial platform...
February 20, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38384763/increasing-vineyard-sustainability-innovating-a-targeted-chitosan-derived-biocontrol-solution-to-induce-grapevine-resistance-against-downy-and-powdery-mildews
#9
JOURNAL ARTICLE
Daphnée Brulé, Marie-Claire Héloir, Thibault Roudaire, Jérémy Villette, Silvère Bonnet, Yoann Pascal, Benoît Darblade, Philippe Crozier, Philippe Hugueney, Véronique Coma, Benoit Poinssot
The European Green Deal aims to reduce the pesticide use, notably by developing biocontrol products to protect crops from diseases. Indeed, the use of significant amounts of chemicals negatively impact the environment such as soil microbial biodiversity or groundwater quality, and human health. Grapevine ( Vitis vinifera ) was selected as one of the first targeted crop due to its economic importance and its dependence on fungicides to control the main damaging diseases worldwide: grey mold, downy and powdery mildews...
2024: Frontiers in Plant Science
https://read.qxmd.com/read/38366943/expanding-the-synthetic-biology-toolbox-of-cupriavidus-necator-for-establishing-fatty-acid-production
#10
JOURNAL ARTICLE
Shivangi Mishra, Paul M Perkovich, Wayne P Mitchell, Maya Venkataraman, Brian Pfleger
The Gram-negative beta-proteobacterium Cupriavidus necator is a chemolithotroph that can convert CO2 into biomass. C. necator has been engineered to produce a variety of high-value chemicals in the past. However, there is still a lack of a well-characterized toolbox for gene expression and genome engineering. Development and optimization of biosynthetic pathways in metabolically engineered microorganisms necessitates control of gene expression via functional genetic elements such as promoters, ribosome binding sites (RBSs), and codon-optimization...
February 16, 2024: Journal of Industrial Microbiology & Biotechnology
https://read.qxmd.com/read/38366578/transcription-factors-vviwrky10-and-vviwrky30-co-regulate-powdery-mildew-resistance-in-grapevine
#11
JOURNAL ARTICLE
Min Zhou, Hongyan Wang, Xuena Yu, Kaicheng Cui, Yang Hu, Shunyuan Xiao, Ying-Qiang Wen
Grapevine (Vitis vinifera) is an economically important fruit crop worldwide. The widely cultivated grapevine is susceptible to powdery mildew caused by Erysiphe necator. In this study, we used CRISPR-Cas9 to simultaneously knock out VviWRKY10 and VviWRKY30 encoding two transcription factors reported to be implicated in defense regulation. We generated 53 wrky10 single mutant transgenic plants and 15 wrky10 wrky30 double mutant transgenic plants. In a 2-year field evaluation of powdery mildew resistance, the wrky10 mutants showed strong resistance, while the wrky10 wrky30 double mutants showed moderate resistance...
February 15, 2024: Plant Physiology
https://read.qxmd.com/read/38360657/n-terminal-truncation-of-phac-bp-m-cpf4-and-its-effect-on-pha-production
#12
JOURNAL ARTICLE
Soon Zher Neoh, Hua Tiang Tan, Chanaporn Trakunjae, Min Fey Chek, Pilanee Vaithanomsat, Toshio Hakoshima, Kumar Sudesh
BACKGROUND: Among the polyhydroxyalkanoate (PHA), poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)] is reported to closely resemble polypropylene and low-density polyethylene. Studies have shown that PHA synthase (PhaC) from mangrove soil (PhaCBP-M-CPF4 ) is an efficient PhaC for P(3HB-co-3HHx) production and N-termini of PhaCs influence its substrate specificity, dimerization, granule morphology, and molecular weights of PHA produced. This study aims to further improve PhaCBP-M-CPF4 through N-terminal truncation...
February 15, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38350870/establishing-crispri-for-programmable-gene-repression-and-genome-evolution-in-cupriavidus-necator
#13
JOURNAL ARTICLE
Zhijiao Wang, Haojie Pan, Sulin Ni, Zhongjian Li, Jiazhang Lian
Cupriavidus necator H16 is a "Knallgas" bacterium with the ability to utilize various carbon sources and has been employed as a versatile microbial cell factory to produce a wide range of value-added compounds. However, limited genome engineering, especially gene regulation methods, has constrained its full potential as a microbial production platform. The advent of CRISPR/Cas9 technology has shown promise in addressing this limitation. Here, we developed an optimized CRISPR interference (CRISPRi) system for gene repression in C...
February 13, 2024: ACS Synthetic Biology
https://read.qxmd.com/read/38346683/crispr-copies-an-in-silico-platform-for-discovery-of-neutral-integration-sites-for-crispr-cas-facilitated-gene-integration
#14
JOURNAL ARTICLE
Aashutosh Girish Boob, Zhixin Zhu, Pattarawan Intasian, Manan Jain, Vassily Andrew Petrov, Stephan Thomas Lane, Shih-I Tan, Guanhua Xun, Huimin Zhao
The CRISPR/Cas system has emerged as a powerful tool for genome editing in metabolic engineering and human gene therapy. However, locating the optimal site on the chromosome to integrate heterologous genes using the CRISPR/Cas system remains an open question. Selecting a suitable site for gene integration involves considering multiple complex criteria, including factors related to CRISPR/Cas-mediated integration, genetic stability, and gene expression. Consequently, identifying such sites on specific or different chromosomal locations typically requires extensive characterization efforts...
February 13, 2024: Nucleic Acids Research
https://read.qxmd.com/read/38288465/prevalence-of-intestinal-parasites-and-comparison-of-detection-techniques-for-soil-transmitted-helminths-among-newly-arrived-expatriate-labors-in-jeddah-saudi-arabia
#15
JOURNAL ARTICLE
Mohammad F Al-Refai, Majed H Wakid
BACKGROUND: Diversity in clinical signs and symptoms are associated with soil transmitted diseases (STD), which are spread to humans by intestinal worms and transmitted in a variety of ways. There is a need for the present study, which aimed to investigate the prevalence of intestinal parasites and to compare between the common detection techniques for soil-transmitted helminths (STHs) among newly arrived expatriate labors in Jeddah, Saudi Arabia. METHODS: A total of 188 stool samples were analyzed by macroscopic examination, and microscopic examination using direct iodine smear and the formal ether sedimentation technique...
2024: PeerJ
https://read.qxmd.com/read/38267563/the-osteoinductive-potential-of-different-root-filling-materials-in-a-rat-femur-model
#16
JOURNAL ARTICLE
Seçkin Aksu, Ebru Delikan, Ayşe Özcan Küçük, Zehra Demiray Asoğlu, Şakir Necat Yılmaz
In pediatric dentistry, the enduring success of root-end filling materials employed for the establishment of apical plugs in immature teeth undergoing endodontic intervention is contingent upon their possessing a robust osteoinductive capacity. Hence, the primary objective of this study was to histologically assess the osteoinductive potential of four distinct dental materials, specifically recommended for retrograde filling applications, utilizing an animal hard tissue model. Within the designed experimental model, two bone defects measuring 2 mm in diameter and 2 mm in depth were created in both femurs of a cohort comprising 21 male Wistar albino rats...
January 24, 2024: Scientific Reports
https://read.qxmd.com/read/38266286/geographic-distribution-of-human-infections-with-zoonotic-ancylostoma-ceylanicum-and-anthropophilic-hookworms-in-ecuador-a-retrospective-analysis-of-archived-stool-samples
#17
JOURNAL ARTICLE
Dayana Aguilar-Rodríguez, Victor Seco-Hidalgo, Andrea Lopez, Natalia Romero-Sandoval, Manuel Calvopiña, Angel Guevara, Lucy Baldeón, Alejandro Rodríguez, Rojelio Mejia, Thomas B Nutman, William J Sears, Philip J Cooper
Zoonotic human infections with Ancylostoma ceylanicum have recently been reported in the Americas. We used archived human stool samples to study the geographic distribution of human infections with A. ceylanicum and anthropophilic hookworms in different geoclimatic regions (coastal, Andean, and Amazon) of Ecuador. We analyzed retrospectively archived human stool samples from five studies previously screened for hookworm infection by microscopy, of which four included hookworm-positive samples only and one involved hookworm-negative samples to increase geographic distribution of sampling...
January 23, 2024: American Journal of Tropical Medicine and Hygiene
https://read.qxmd.com/read/38203719/optimizing-hexose-utilization-pathways-of-cupriavidus-necator-for-improving-growth-and-l-alanine-production-under-heterotrophic-and-autotrophic-conditions
#18
JOURNAL ARTICLE
Lei Wang, Huiying Luo, Bin Yao, Junhu Yao, Jie Zhang
Cupriavidus necator is a versatile microbial chassis to produce high-value products. Blocking the poly-β-hydroxybutyrate synthesis pathway (encoded by the phaC1AB1 operon) can effectively enhance the production of C. necator , but usually decreases cell density in the stationary phase. To address this problem, we modified the hexose utilization pathways of C. necator in this study by implementing strategies such as blocking the Entner-Doudoroff pathway, completing the phosphopentose pathway by expressing the gnd gene (encoding 6-phosphogluconate dehydrogenase), and completing the Embden-Meyerhof-Parnas pathway by expressing the pfkA gene (encoding 6-phosphofructokinase)...
December 31, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38180119/coupling-of-co2-electrolysis-with-parallel-and-semi-automated-biopolymer-synthesis-ex-cell-and-without-downstream-processing
#19
JOURNAL ARTICLE
Ida Dinges, Ina Depentori, Lisa Gans, Dirk Holtmann, Siegfried R Waldvogel, Markus Stöckl
Important improvements have been achieved in developing the coupling of electrochemical CO2 reduction to formate with its subsequent microbial conversion to polyhydroxybutyrate (PHB) by Cupriavidus necator. The CO2 based formate electrosynthesis was optimised by electrolysis parameter adjustment and application of Sn based gas diffusion electrodes reaching almost 80% Faradaic efficiency at 150 mA cm-2. Thereby, catholyte with the high formate concentration of 441 ± 9 mmol L-1 was generated as feedstock without intermediate downstream processing for semi-automated formate feeding into a fed-batch reactor system...
January 5, 2024: ChemSusChem
https://read.qxmd.com/read/38172920/co-2-based-production-of-phytase-from-highly-stable-expression-plasmids-in-cupriavidus-necator-h16
#20
JOURNAL ARTICLE
Simon Arhar, Thomas Rauter, Holly Stolterfoht-Stock, Vera Lambauer, Regina Kratzer, Margit Winkler, Marianna Karava, Robert Kourist, Anita Emmerstorfer-Augustin
BACKGROUND: Existing plasmid systems offer a fundamental foundation for gene expression in Cupriavidus necator; however, their applicability is constrained by the limitations of conjugation. Low segregational stabilities and plasmid copy numbers, particularly in the absence of selection pressure, pose challenges. Phytases, recognized for their widespread application as supplements in animal feed to enhance phosphate availability, present an intriguing prospect for heterologous production in C...
January 3, 2024: Microbial Cell Factories
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