Mingyan Wang, Naiyu Jiang, Yuanchao Xu, Xinxiu Chen, Cui Wang, Chuangjiang Wang, Shiqi Wang, Kuipeng Xu, Sen Chai, Qing Yu, Zhonghua Zhang, Huimin Zhang
Many biotic or abiotic factors such as CPPU (N-(2-chloro-pyridin-4-yl)-N'-phenylurea), a growth regulator of numerous crops, can induce bitterness in cucurbits. In melon, cucurbitacin B is the major compound leading to bitterness. However, the molecular mechanism underlying CuB biosynthesis in response to different conditions remains unclear. Here, we identified a set of genes involved in CPPU-induced CuB biosynthesis in melon fruit and proposed CmBr gene as the major regulator. Using CRISPR/Cas9 gene editing, we confirmed CmBr's role in regulating CuB biosynthesis under CPPU treatment...
May 30, 2024: Plant Biotechnology Journal