Dian Ekayanti Astari, Muhammad Nasrum Massi, Rina Masadah, Marhaen Hardjo, Rosdiana Natzir, Michael Erlichster, Gursharan Chana, Efstratios Skafidas, Zeba Islam Seraj, Sabrina M Elias, Gita Vita Soraya
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a molecular amplification method that can detect SARS-CoV-2 in a shorter time than the current gold-standard molecular diagnostic reverse transcription-polymerase chain reaction (RT-PCR). However, previously developed RT-LAMP assays have mostly relied on highly subjective visual colorimetric interpretation. In this study, an RT-LAMP assay was developed with quantitative measurement of reaction pH using a novel portable pH biosensor compared to qualitative colorimetric interpretation and gel electrophoresis, with 57 clinical COVID-19 samples used for validation of the test...
April 25, 2024: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica