Zhen Sun, Lili Zhang, Kai Yin, Guangyao Zang, Yongjiang Qian, Xiang Mao, Lihua Li, Qing Jing, Zhongqun Wang
BACKGROUND AND AIMS: Arterial calcification is the predictor of cardiovascular risk in diabetic patients. Nε -carboxymethyl-lysine (CML), a toxic metabolite, is associated with accelerated vascular calcification in diabetes mellitus (DM). However, the mechanism remains elusive. This study aims to explore the key regulators involved in CML-induced vascular calcification in DM. METHODS: We used Western blot and immuno-staining to test the expression and localization of nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) in human samples, a diabetic apolipoprotein E-deficient (ApoE-/- ) mouse model, and a vascular smooth muscle cells (VSMC) model...
July 2023: Atherosclerosis