Qiaoyun Zhao, Conghua Song, Ke Wang, Donghong Li, Yang Yang, Dongsheng Liu, Le Wang, Nanjin Zhou, Yong Xie
Disease outcome is associated with virulence factors of Helicobacter pylori (H. pylori), which are partially attributed to the outer membrane protein (OMP). This study aimed to investigate the correlation between the four OMP genes (babA, oipA, sabA, and homB) and gastroduodenal diseases. One hundred and seventy-seven H. pylori strains were isolated from Chinese patients with different gastroduodenal diseases (49 chronic gastritis, 19 gastric ulcer, 33 gastric cancer, and 76 duodenal ulcer), 94 of which contained pathological information (41 superficial gastritis, 24 intestinal hyperplasia, and 29 gastric adenocarcinoma)...
March 26, 2020: Medical Microbiology and Immunology
Jacqueline Fröhlich, Adam Grundhoff
Kaposi sarcoma-associated herpesvirus (KSHV) is the etiologic agent of several malignancies of endothelial and B-cell origin. The fact that latently infected tumor cells in these malignancies do not express classical viral oncogenes suggests that pathogenesis of KSHV-associated disease results from multistep processes that, in addition to constitutive viral gene expression, may require accumulation of cellular alterations. Heritable changes of the epigenome have emerged as an important co-factor that contributes to the pathogenesis of many non-viral cancers...
March 26, 2020: Seminars in Immunopathology
Devon E McMahon, Toby Maurer, Esther E Freeman
No abstract text is available yet for this article.
March 2020: JCO global oncology
Siamak Farhadi, Ahmad Moieni, Naser Safaie, Mohammad Sadegh Sabet, Mina Salehi
Paclitaxel is the top-selling chemotherapeutic drug used for the treatment of lung, ovarian and breast cancer as well as Kaposi's sarcoma. Cell suspension culture (CSC) of Corylus avellana has been addressed as a promising alternative for producing paclitaxel. In this study, endophytic fungus strain YEF33 was isolated from Taxus baccata and identified as Coniothyrium palmarum. The effects of the elicitors derived from this fungus including cell extract, culture filtrate and cell wall (CW) and also chitin, alone or in combination with Methyl-β-Cyclodextrin (MBCD), on paclitaxel biosynthesis in C...
March 25, 2020: Scientific Reports
Un Yung Choi, Jae Jin Lee, Angela Park, Wei Zhu, Hye-Ra Lee, Youn Jung Choi, Ji-Seung Yoo, Claire Yu, Pinghui Feng, Shou-Jiang Gao, Shaochen Chen, Hyungjin Eoh, Jae U Jung
Three-dimensional (3D) cell culture is well documented to regain intrinsic metabolic properties and to better mimic the in vivo situation than two-dimensional (2D) cell culture. Particularly, proline metabolism is critical for tumorigenesis since pyrroline-5-carboxylate (P5C) reductase (PYCR/P5CR) is highly expressed in various tumors and its enzymatic activity is essential for in vitro 3D tumor cell growth and in vivo tumorigenesis. PYCR converts the P5C intermediate to proline as a biosynthesis pathway, whereas proline dehydrogenase (PRODH) breaks down proline to P5C as a degradation pathway...
March 25, 2020: Proceedings of the National Academy of Sciences of the United States of America
Yuanyuan Wan, Zhen Wang, Jichun Xia, Shulin Shen, Mingwei Guan, Meichen Zhu, Cailin Qiao, Fujun Sun, Ying Liang, Jiana Li, Kun Lu, Cunmin Qu
Phosphorus transporter ( PHT ) genes encode H2 PO4- /H+ co-transporters that absorb and transport inorganic nutrient elements required for plant development and growth and protect plants from heavy metal stress. However, little is known about the roles of PHTs in Brassica compared to Arabidopsis thaliana . In this study, we identified and extensively analyzed 336 PHTs from three diploid ( B. rapa, B. oleracea, and B. nigra ) and two allotetraploid ( B. juncea and B. napus ) Brassica species. We categorized the PHTs into five phylogenetic clusters ( PHT1 - PHT5 ), including 201 PHT1 homologs, 15 PHT2 homologs, 40 PHT3 homologs, 54 PHT4 homologs, and 26 PHT5 homologs, which are unevenly distributed on the corresponding chromosomes of the five Brassica species...
March 23, 2020: International Journal of Molecular Sciences
Veeresh Kumar Sali, Sugumar Mani, G Meenaloshani, Anbumani Velmurugan Ilavarasi, Hannah R Vasanthi
Cholest-4-ene-3,6-dione (KS) is a cholesterol oxidation product which exhibits anti-proliferative activity. However, its precise mechanism of action remains unknown. In this study, the effects of KS on AKR1C3 inhibition and anti-proliferative activities were investigated in the hormone-dependent MCF-7 breast cancer cells. We identified that KS arrested the enzymatic conversion of estrone to 17-β estradiol, by inhibiting AKR1C3 in intact MCF-7 cells. The anti-proliferative effects of KS were evaluated by MTT assay, acridine orange and ethidium bromide dual staining, cell cycle analysis and Western blotting...
March 21, 2020: Steroids
Peng Wu, Zhong-Xiuzi Gao, Dan-Dan Zhang, Xin-Peng Duan, Andrew S Terker, Dao-Hong Lin, David H Ellison, Wen-Hui Wang
Background Angiotensin II stimulates epithelial Na+ channel (ENaC) by aldosterone-independent mechanism. We now test the effect of angiotensin II on ENaC in the distal convoluted tubule (DCT) and cortical collecting duct (CCD) of wild-type (WT) and kidney-specific mineralocorticoid receptor knockout mice (KS-MR-KO). Methods and Results We used electrophysiological, immunoblotting and renal-clearance methods to examine the effect of angiotensin II on ENaC in KS-MR-KO and wild-type mice. High K+ intake stimulated ENaC in the late DCT/early connecting tubule (DCT2/CNT) and in the CCD whereas low sodium intake stimulated ENaC in the CCD but not in the DCT2/CNT...
April 7, 2020: Journal of the American Heart Association
Tao Yu, Bing-Hong Huang, Yuyang Zhang, Pei-Chun Liao, Jun-Qing Li
Thuja sutchuenensis is a critically endangered tertiary relict species of Cupressaceae from southwestern China. We sequenced the complete chloroplast (cp) genome of T . sutchuenensis , showing the genome content of 129,776 bp, 118 unique genes including 82 unique protein-coding genes, 32 tRNA genes, and 4 rRNA genes. The genome structures, gene order, and GC content are similar to other typical gymnosperm cp genomes. Thirty-eight simple sequence repeats were identified in the T . sutchuenensis cp genome. We also found an apparent inversion between trnT and psbK between genera Thuja and Thujopsis ...
March 2020: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Salomé Manska, Rionna Octaviano, Cyprian C Rossetto
Nucleoside analogs are a valuable experimental tool. Incorporation of these molecules into newly synthesized DNA (i.e. pulse-labeling) is used to monitor cell proliferation or to isolate nascent DNA. Some of the most common nucleoside analogs used for pulse-labeling of DNA in cells are the deoxypyrimidine analogs 5-ethynyl-2'-deoxyuridine (EdU) and 5-ethynyl-2'-deoxycytidine (EdC). Click chemistry enables conjugation of an azide molecule tagged with a fluorescent dye or biotin to the alkyne of the analog, which can then be used to detect incorporation of EdU and EdC into DNA...
March 23, 2020: Journal of Biological Chemistry
Valerio Papa, Maria Cristina Giustiniani, Loris Riccardo Lopetuso, Alfredo Papa
BACKGROUND: Kaposi's sarcoma (KS) is a rare vascular tumor associated with human herpesvirus (HHV)-8 infection. One of the variants of KS is defined iatrogenic and is overall reported in transplanted patient but also, although less frequently, in patients treated with long-standing immunosuppressive therapy, such as in inflammatory bowel disease including ulcerative colitis and Crohn's disease. CASE PRESENTATION: Herein, we report the first case of KS in a human immunodeficiency virus (HIV)-negative 47-year old male with UC after treatment with the α4-β7 integrin inhibitor vedolizumab (VDZ)...
March 24, 2020: BMC Gastroenterology
Syed Saad Bin Qasim, Muhammad Sohail Zafar, Fayyez Hussain Niazi, Majid Alshahwan, Hanan Abdel Ks, Umer Daood
Design and development of novel therapeutic strategies to regenerate lost tissue structure and function is a serious clinical hurdle for researchers. Traditionally much of the research is dedicated in optimizing properties of scaffolds. Current synthetic biomaterials remain rudimentary in comparison to their natural counterparts. The ability to incorporate biologically inspired elements into the design of synthetic materials has advanced with time. Recent reports suggest that functionally graded material mimicking the natural tissue morphology can have a more exaggerated response on the targeted tissue...
March 23, 2020: Journal of Biomaterials Science. Polymer Edition
Biagio Cangiano, Du Soon Swee, Richard Quinton, Marco Bonomi
A genetic basis of congenital isolated hypogonadotropic hypogonadism (CHH) can be defined in almost 50% of cases, albeit not necessarily the complete genetic basis. Next-generation sequencing (NGS) techniques have led to the discovery of a great number of loci, each of which has illuminated our understanding of human gonadotropin-releasing hormone (GnRH) neurons, either in respect of their embryonic development or their neuroendocrine regulation as the "pilot light" of human reproduction. However, because each new gene linked to CHH only seems to underpin another small percentage of total patient cases, we are still far from achieving a comprehensive understanding of the genetic basis of CHH...
March 21, 2020: Human Genetics
Mohammad Amro, Kanaan Mansoor, Ahmed Amro, Kelechukwu Okoro, Paul I Okhumale
Protamine sulfate is considered a "life-saving" antidote for heparinized patients with major bleeds. Although the beneficial attributes and application of protamine sulfate in various clinical settings cannot be argued, it also has an impressive side-effect profile. Kounis syndrome (KS) is an acute coronary syndrome in the setting of an allergic reaction, which can be induced by numerous allergens. Herein, we report a case of KS secondary to the use of protamine sulfate after cryoablation for atrial fibrillation...
February 12, 2020: Curēus
Wiltine G Moerman-van den Brink, Loes van Aken, Els M L Verschuur, Serge J W Walvoort, Yvonne C M Rensen, Jos I M Egger, Roy P C Kessels
Objective : Patients with Korsakoff's syndrome (KS) show executive dysfunction and neuropsychiatric symptoms. This study investigates whether specific executive subcomponents (shifting, updating, and inhibition) predict variance in neuropsychiatric symptoms. We hypothesized that shifting deficits, in particular, are associated with neuropsychiatric symptoms. Method : Forty-seven patients participated (mean age 61.5; 11 women). Executive function (EF) was measured using six component-specific tasks. Neuropsychiatric symptoms were measured with the Neuropsychiatric Inventory - Questionnaire (NPI-Q)...
March 19, 2020: Clinical Neuropsychologist
Vieri Grandi, Irene De Francesco
New England Journal of Medicine, Volume 382, Issue 12, March 2020.
March 19, 2020: New England Journal of Medicine
Maggie L Chow, Ashley J Crew, Nicole N Harter
No abstract text is available yet for this article.
February 2020: Cutis; Cutaneous Medicine for the Practitioner
Mohammad A Albanghali, Basim A Othman
BACKGROUND: Sexually transmitted diseases (STDs) remain one of the most important health challenges in not only developing countries but also developed countries. Discussing STDs in the Saudi society is considered taboo, as social factors and ethics give rise to many obstacles. This study evaluates the knowledge of STDs among young adult students enrolled in Albaha University. METHODS: This research is a cross-sectional study involving 1902 young adult students registered at Albaha University...
March 13, 2020: International Journal of Environmental Research and Public Health
Huiting Ruan, Min Li, Zhenhai Li, Jiajie Huang, Weiyuan Chen, Jijia Sun, Li Liu, Keshu Zou
Mitochondrial genome is a powerful molecule marker to explore phylogenetic relationships and reveal molecular evolution in ichthyological studies. Gerres species play significant roles in marine fishery, but its evolution has received little attention. To date, only two Gerres mitochondrial genomes were reported. In the present study, three mitogenomes of Gerres ( Gerres filamentosus , Gerres erythrourus , and Gerres decacanthus ) were systemically investigated. The lengths of the mitogenome sequences were 16,673, 16,728, and 16,871 bp for G...
March 9, 2020: International Journal of Molecular Sciences
Jason Nomburg, Susan Bullman, Sun Sook Chung, Katsuhiro Togami, Mark A Walker, Gabriel K Griffin, Elizabeth A Morgan, Nicole R LeBoeuf, James A DeCaprio, Matthew Meyerson, Andrew A Lane
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a hematologic malignancy believed to originate from plasmacytoid dendritic cells (pDCs), the immune cells responsible for producing type 1 interferons during infection. Nearly all patients with BPDCN have prominent skin involvement, with cutaneous infiltration occupying the dermis and subcutis. One half of patients present with BPDCN cells only in the skin, with no evidence of disease elsewhere. Because normal pDCs are rare or absent in cutaneous sites, and they only traffic to the skin after activation by pathogen or inflammation, our aim was to determine if a microorganism is associated with BPDCN...
March 24, 2020: Blood Advances
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