Gabriela Venturini, Juliana M Alvim, Kallyandra Padilha, Christopher N Toepfer, Joshua M Gorham, Lauren K Wasson, Diogo Biagi, Sergio Schenkman, Valdemir M Carvalho, Jessica S Salgueiro, Karina H M Cardozo, Jose E Krieger, Alexandre C Pereira, Jonathan G Seidman, Christine E Seidman
INTRODUCTION: Chagas cardiomyopathy, a disease caused by Trypanosoma cruzi ( T. cruzi ) infection, is a major contributor to heart failure in Latin America. There are significant gaps in our understanding of the mechanism for infection of human cardiomyocytes, the pathways activated during the acute phase of the disease, and the molecular changes that lead to the progression of cardiomyopathy. METHODS: To investigate the effects of T. cruzi on human cardiomyocytes during infection, we infected induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) with the parasite and analyzed cellular, molecular, and metabolic responses at 3 hours, 24 hours, and 48 hours post infection (hpi) using transcriptomics (RNAseq), proteomics (LC-MS), and metabolomics (GC-MS and Seahorse) analyses...
2023: Frontiers in Cellular and Infection Microbiology