keyword
https://read.qxmd.com/read/38532772/discontinuous-translocation-of-a-luciferase-protein-beyond-graft-junction-in-tobacco
#21
JOURNAL ARTICLE
Taira Miyahara, Hitomi Ohkubo, Yukiko Umeyama, Taichi Oguchi, Takumi Ogawa, Daisaku Ohta, Tomofumi Mochizuki, Hiroaki Kodama
Transgrafting, a grafting technique that uses both genetically modified (GM) and non-GM plants, is a novel plant breeding technology that can be used to improve the efficiency of crop cultivation without introducing foreign genes into the edible parts of non-GM plants. This technique can facilitate the acquisition of disease resistance and/or increased yield. However, the translocation of low-molecular-weight compounds, ribonucleic acid (RNA), and proteins through graft junctions raises a potential safety risk for food crops...
March 2024: Food safety
https://read.qxmd.com/read/38532644/cotton-blh1-and-knox6-antagonistically-modulate-fiber-elongation-via-the-regulation-of-linolenic-acid-biosynthesis
#22
JOURNAL ARTICLE
Tingting Jia, Huiqin Wang, Shiyan Cui, Zihan Li, Yongcui Shen, Hongbin Li, Guanghui Xiao
BEL1-LIKE HOMEODOMAIN (BLH) proteins are known to function in various plant developmental processes. However, the role of BLHs in regulating plant cell elongation is still unknown. Here, we identified a BLH gene, GhBLH1, that positively regulates fiber cell elongation. Combined transcriptomic and biochemical analyses revealed that GhBLH1 enhances linolenic acid accumulation to promote cotton fiber cell elongation by activating the transcription of GhFAD7A-1 via the binding of the POX domain of GhBLH1 to the TGGA cis-element in the GhFAD7A-1 promoter...
March 25, 2024: Plant communications
https://read.qxmd.com/read/38532479/improving-crispr-cas9-directed-faithful-transgene-integration-outcomes-by-reducing-unwanted-random-dna-integration
#23
JOURNAL ARTICLE
Rio Hermantara, Laura Richmond, Aqeel Faisal Taqi, Sabari Chilaka, Valentine Jeantet, Ileana Guerrini, Katherine West, Adam West
BACKGROUND: The field of genome editing has been revolutionized by the development of an easily programmable editing tool, the CRISPR-Cas9. Despite its promise, off-target activity of Cas9 posed a great disadvantage for genome editing purposes by causing DNA double strand breaks at off-target locations and causing unwanted editing outcomes. Furthermore, for gene integration applications, which introduce transgene sequences, integration of transgenes to off-target sites could be harmful, hard to detect, and reduce faithful genome editing efficiency...
March 26, 2024: Journal of Biomedical Science
https://read.qxmd.com/read/38532242/ace2-rescues-sepsis-associated-encephalopathy-by-reducing-inflammation-oxidative-stress-and-neuronal-apoptosis-via-the-nrf2-sestrin2-signaling-pathway
#24
JOURNAL ARTICLE
Ya Li, Tian-Tian Wan, Jia-Xin Li, Xue Xiao, Lei Liu, Hui-Hua Li, Shu-Bin Guo
Neuroinflammation and oxidative stress contribute to the progression of sepsis-associated encephalopathy (SAE). Angiotensin-converting enzyme 2 (ACE2) is considered to be a neuroprotective factor due to its anti-inflammatory and antioxidant properties. However, the role of ACE2 on myeloid cells in regulating SAE and the underlying mechanism warrants further exploration. SAE was induced in ACE2 transgenic (TG), knockout (KO), and bone marrow (BM) chimeric mice by cecal ligation and puncture (CLP). The expression levels of apoptosis-, oxidation- and neuroinflammation-associated mediators and morphological changes were monitored by quantitative real-time PCR analyses and histological examinations in the cortex of septic mice...
March 27, 2024: Molecular Neurobiology
https://read.qxmd.com/read/38532219/removal-of-high-lead-concentration-by-hydroponic-cultures-of-normal-and-transformed-plants-of-scirpus-americanus-pers
#25
JOURNAL ARTICLE
Elizabeth Esquivel-Ramos, Ma Catalina Alfaro-de la Torre, María Del Socorro Santos-Díaz
Lead is a very toxic metal which affects human health. An alternative to remove it from contaminated water is the use of macrophytes, as Scirpus americanus Pers. This species is tolerant to salt and metals and has high biomass. The present research analyzed the capacity of hydroponic cultures of normal and transgenic plants (line T12) from S. americanus to remove high concentrations of lead. The antioxidant response of plants to metal exposure was also measured. The MINTEQ3.1 program was used to define the media composition in order to have the metal available to the plants...
March 27, 2024: Environmental Science and Pollution Research International
https://read.qxmd.com/read/38532087/callus-induction-followed-by-regeneration-and-hairy-root-induction-in-common-buckwheat
#26
JOURNAL ARTICLE
Denis O Omelchenko, Elena S Glagoleva, Anna Y Stepanova, Maria D Logacheva
This section describes a set of methods for callus induction followed by the successful regeneration of whole plants and obtaining a culture of transgenic hairy roots from buckwheat plants (Fagopyrum esculentum Moench.). Callus induction and regeneration are key steps for many biotechnological, genetic, and breeding approaches, such as genetic modification, production of biologically active compounds, and propagation of valuable germplasm. Induction of hairy roots using Agrobacterium rhizogenes is also an important tool for functional gene research and plant genome modification...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38532083/identification-of-a-sugarcane-bacilliform-virus-promoter-that-is-activated-by-drought-stress-in-plants
#27
JOURNAL ARTICLE
Sheng-Ren Sun, Xiao-Bin Wu, Jian-Sheng Chen, Mei-Ting Huang, Hua-Ying Fu, Qin-Nan Wang, Philippe Rott, San-Ji Gao
Sugarcane (Saccharum spp.) is an important sugar and biofuel crop in the world. It is frequently subjected to drought stress, thus causing considerable economic losses. Transgenic technology is an effective breeding approach to improve sugarcane tolerance to drought using drought-inducible promoter(s) to activate drought-resistance gene(s). In this study, six different promoters were cloned from sugarcane bacilliform virus (SCBV) genotypes exhibiting high genetic diversity. In β-glucuronidase (GUS) assays, expression of one of these promoters (PSCBV-YZ2060 ) is similar to the one driven by the CaMV 35S promoter and >90% higher compared to the other cloned promoters and Ubi1...
March 26, 2024: Communications Biology
https://read.qxmd.com/read/38531838/tissue-specific-silencing-of-integrated-transgenes-achieved-through-endogenous-rna-interference-in-caenorhabditis-elegans
#28
JOURNAL ARTICLE
Siyu Chen, Weihong Liu, Lei Xiong, Zhiju Tao, Di Zhao
Transgene silencing is a common phenomenon observed in Caenorhabditis elegans , particularly in the germline, but the precise mechanisms underlying this process remain elusive. Through an analysis of the transcription factors profile of C. elegans , we discovered that the expression of several transgenic reporter lines exhibited tissue-specific silencing, specifically in the intestine of C. elegans . Notably, this silencing could be reversed in mutants defective in endogenous RNA interference (RNAi). Further investigation using knock-in strains revealed that these intestine-silent genes were indeed expressed in vivo , indicating that the organism itself regulates the intestine-specific silencing...
January 2024: RNA Biology
https://read.qxmd.com/read/38531640/-initial-characterization-of-a-transgenic-mouse-with-overexpression-of-the-human-h-1-histamine-receptor-on-the-heart
#29
JOURNAL ARTICLE
Lina Maria Rayo Abella, Hannes Jacob, Max Keller, Lisa Schindler, Steffen Pockes, Sebastian Pitzl, Jan Klimas, Katarina Hadova, Sarah Schneider, Igor B Buchwalow, CongYu Jin, Pertti Panula, Uwe Kirchhefer, Joachim Neumann, Ulrich Gergs
There is controversy whether H1 -histamine receptors increase or decrease contractility in the mammalian heart. Therefore, we have generated mice that overexpress the human H1 -histamine receptor in the heart (H1 -TG). We hypothesized that histamine would either increase or decrease force of contraction and beating rate compared to littermate wild type mice (WT). In H1 -TG mice, we ascertained the presence of H1 -histamine receptors by autoradiography of atrium and ventricle using [3 H]mepyramine. The messenger RNA for human H1 -histamine-receptors was present in cardiac samples from H1 -TG and absent from WT...
March 26, 2024: Journal of Pharmacology and Experimental Therapeutics
https://read.qxmd.com/read/38531482/dipeptidyl-peptidase-9-regulates-the-dynamics-of-tumorigenesis-and-metastasis-in-breast-cancer
#30
JOURNAL ARTICLE
Lisa Heß, Kazeera Aliar, Barbara T Grünwald, Ricarda Griffin, Alina Lozan, Mariel Knöller, Rama Khokha, Tilman Brummer, Thomas Reinheckel
The cytosolic dipeptidyl-aminopeptidase 9 (DPP9) cleaves protein N-termini post-proline or -alanine. Our analysis of DPP9 mRNA expression from the TCGA 'breast cancer' data set revealed that low/intermediate DPP9 levels are associated with poor overall survival of breast cancer patients. To unravel the impact of DPP9 on breast cancer development and progression, the transgenic MMTV-PyMT mouse model of metastasizing breast cancer was used. In addition, tissue- and time-controlled genetic deletion of DPP9 by the Cre-loxP recombination system was done...
March 24, 2024: Biochimica et Biophysica Acta. Molecular Basis of Disease
https://read.qxmd.com/read/38531358/the-wip6-transcription-factor-too-many-laterals-specifies-vein-type-in-c-4-and-c-3-grass-leaves
#31
JOURNAL ARTICLE
Daniela Vlad, Maricris Zaidem, Chiara Perico, Olga Sedelnikova, Samik Bhattacharya, Jane A Langdale
Grass leaves are invariantly strap shaped with an elongated distal blade and a proximal sheath that wraps around the stem. Underpinning this shape is a scaffold of leaf veins, most of which extend in parallel along the proximo-distal leaf axis. Differences between species are apparent both in the vein types that develop and in the distance between veins across the medio-lateral leaf axis. A prominent engineering goal is to increase vein density in leaves of C3 photosynthesizing species to facilitate the introduction of the more efficient C4 pathway...
March 19, 2024: Current Biology: CB
https://read.qxmd.com/read/38531063/parallel-continuous-monitoring-and-quantification-of-programmed-cell-death-in-plant-tissue
#32
JOURNAL ARTICLE
Alexander Silva Pinto Collins, Hasan Kurt, Cian Duggan, Yasin Cotur, Philip Coatsworth, Atharv Naik, Matti Kaisti, Tolga Bozkurt, Firat Güder
Accurate quantification of hypersensitive response (HR) programmed cell death is imperative for understanding plant defense mechanisms and developing disease-resistant crop varieties. Here, a phenotyping platform for rapid, continuous-time, and quantitative assessment of HR is demonstrated: Parallel Automated Spectroscopy Tool for Electrolyte Leakage (PASTEL). Compared to traditional HR assays, PASTEL significantly improves temporal resolution and has high sensitivity, facilitating detection of microscopic levels of cell death...
March 26, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://read.qxmd.com/read/38530835/characterisation-of-the-r2r3-myb-subgroup-9-family-of-transcription-factors-in-tomato
#33
JOURNAL ARTICLE
Gwendolyn V Davis, Beverley J Glover
Tomato (Solanum lycopersicum) has many epidermal cell outgrowths including conical petal cells and multiple types of trichomes. These include the anther-specific trichome mesh which holds the anthers connate. The R2R3 Myb Subgroup 9 family of transcription factors is involved in development of epidermal cell outgrowths throughout the angiosperms. No previous study has examined all members of this transcription factor family in a single species. All 7 R2R3 Myb Subgroup 9 genes were isolated from tomato. They were ectopically expressed in tobacco to assess their ability to induce epidermal cell outgrowth...
2024: PloS One
https://read.qxmd.com/read/38530369/igg-hexamers-initiate-complement-dependent-acute-lung-injury
#34
JOURNAL ARTICLE
Simon J Cleary, Yurim Seo, Jennifer J Tian, Nicholas Kwaan, David P Bulkley, Arthur E H Bentlage, Gestur Vidarsson, Éric Boilard, Rolf Spirig, James C Zimring, Mark R Looney
Antibodies can initiate lung injury in a variety of disease states such as autoimmunity, transfusion reactions, or after organ transplantation, but the key factors determining in vivo pathogenicity of injury-inducing antibodies are unclear. Harmful antibodies often activate the complement cascade. A model for how IgG antibodies trigger complement activation involves interactions between IgG Fc domains driving assembly of IgG hexamer structures that activate C1 complexes. The importance of IgG hexamers in initiating injury responses was unclear, so we tested their relevance in a mouse model of alloantibody and complement-mediated acute lung injury...
March 26, 2024: Journal of Clinical Investigation
https://read.qxmd.com/read/38530364/satellite-glial-gpr37l1-and-its-ligand-maresin-1-regulate-potassium-channel-signaling-and-pain-homeostasis
#35
JOURNAL ARTICLE
Sangsu Bang, Changyu Jiang, Jing Xu, Sharat Chandra, Aidan McGinnis, Xin Luo, Qianru He, Yize Li, Zilong Wang, Xiang Ao, Marc Parisien, Lorenna Oliveira Fernandes de Araujo, Sahel Jahangiri Esfahani, Qin Zhang, Raquel Tonello, Temugin Berta, Luda Diatchenko, Ru-Rong Ji
G protein-coupled receptor 37-like 1 (GPR37L1) is an orphan GPCR with largely unknown functions. Here we report that Gpr37l1/GRP37L1 ranks among the most highly expressed GPCR transcripts in mouse and human dorsal root ganglia (DRGs), selectively expressed in satellite glial cells (SGCs). Peripheral neuropathy induced by streptozotoxin (STZ) and paclitaxel (PTX) led to reduced GPR37L1 expression on the plasma membrane expression in mouse and human DRGs. Transgenic mice with Gpr37l1 deficiency exhibited impaired resolution of neuropathic pain symptoms following PTX and STZ-induced pain, whereas overexpression of Gpr37l1 in mouse DRGs reversed pain...
March 26, 2024: Journal of Clinical Investigation
https://read.qxmd.com/read/38530355/rnf4-sustains-myc-driven-tumorigenesis-by-facilitating-dna-replication
#36
JOURNAL ARTICLE
Joonyoung Her, Haiyan Zheng, Samuel F Bunting
The mammalian SUMO-targeted E3 Ubiquitin Ligase, Rnf4, has been reported to act as a regulator of DNA repair, but the importance of RNF4 as a tumor suppressor has not been tested. Using a conditional-knockout mouse model, we deleted Rnf4 in the B cell lineage to test the importance of RNF4 for growth of somatic cells. Although Rnf4 conditional-knockout B cells exhibited substantial genomic instability, Rnf4 deletion caused no increase in tumor susceptibility. In contrast, Rnf4 deletion extended the healthy lifespan of mice expressing an oncogenic c-myc transgene...
March 26, 2024: Journal of Clinical Investigation
https://read.qxmd.com/read/38530081/making-human-hematopoietic-stem-cells-without-transgenes
#37
Luis G Palma, Anna Bigas
Creating hematopoietic stem cells (HSCs) capable of multilineage engraft while possessing the ability to self-renew stands as a pivotal achievement within the field of regenerative medicine. However, achieving the generation of these cells without transgene expression or teratoma formation has not been fully accomplished. In a recent publication featured in Cell Stem Cell , Piau et al. document the production of functional HSCs derived from human-induced pluripotent stem cells (hiPSCs). They achieved this through a one-step differentiation protocol that notably does not require any transgene expression...
March 26, 2024: Cellular Reprogramming
https://read.qxmd.com/read/38529622/rifampin-and-silymarin-mediated-pharmacokinetic-interactions-of-exogenous-and-endogenous-substrates-in-a-transgenic-oatp1b-mouse-model
#38
JOURNAL ARTICLE
Baron J Bechtold, Katherine D Lynch, Victoria O Oyanna, M Ridge Call, Tyler N Graf, Nicholas H Oberlies, John D Clarke
Organic anion-transporting polypeptides (OATP) 1B1 and OATP1B3, encoded by the SLCO gene family of the solute carrier superfamily, are involved in the disposition of many exogenous and endogenous compounds. Preclinical rodent models help assess risks of pharmacokinetic interactions, but interspecies differences in transporter orthologs and expression limit direct clinical translation. An OATP1B transgenic mouse model comprising a rodent Slco1a/1b gene cluster knockout and human SLCO1B1 and SLCO1B3 gene insertions provides a potential physiologically relevant preclinical tool to predict pharmacokinetic interactions...
March 26, 2024: Molecular Pharmaceutics
https://read.qxmd.com/read/38529619/se-methylselenocysteine-ameliorates-mitochondrial-function-by-targeting-both-mitophagy-and-autophagy-in-the-mouse-model-of-alzheimer-s-disease
#39
JOURNAL ARTICLE
Yongli Xie, Xiaoshan Ke, Zhencong Ye, Xuexia Li, Zetao Chen, Jiantao Liu, Ziyi Wu, Qiong Liu, Xiubo Du
Background : Alzheimer's disease (AD) exerts tremendous pressure on families and society due to its unknown etiology and lack of effective treatment options. Our previous study had shown that Se-methylselenocysteine (SMC) improved the cognition and synaptic plasticity of triple-transgenic AD (3 × Tg-AD) mice and alleviated the related pathological indicators. We are dedicated to investigating the therapeutic effects and molecular mechanisms of SMC on mitochondrial function in 3 × Tg-AD mice. Methods : Transmission electron microscopy (TEM), western blotting (WB), mitochondrial membrane potential (Δ Ψ m ), mitochondrial swelling test, and mitochondrial oxygen consumption test were used to evaluate the mitochondrial morphology and function...
March 26, 2024: Food & Function
https://read.qxmd.com/read/38528615/current-and-emerging-gene-therapies-for-haemophilia-a-and-b
#40
JOURNAL ARTICLE
Radoslaw Kaczmarek, Wolfgang Miesbach, Margareth C Ozelo, Pratima Chowdary
INTRODUCTION: After decades of stumbling clinical development, the first gene therapies for haemophilia A and B have been commercialized and have normalized factor (F)VIII and factor (F)IX levels in some individuals in the long term. Several other clinical programs testing adeno-associated viral (AAV) vector gene therapy are at various stages of clinical testing. DISCUSSION: Multiyear follow-up in phase 1/2 and 3 studies showed long-term and sometimes curative but widely variable and unpredictable efficacy...
March 25, 2024: Haemophilia: the Official Journal of the World Federation of Hemophilia
keyword
keyword
110464
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.