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Natalia Cichon, Michal Bijak, Ewelina Synowiec, Elzbieta Miller, Tomasz Sliwinski, Joanna Saluk-Bijak
Oxidative stress plays the most important role in the pathogenesis of stroke. Extremely low frequency electromagnetic field (ELF-EMF) therapy may be complementary in post-stroke therapy, as it modulates oxidative stress. The aim of this study was to evaluate the messenger ribonucleic acid (mRNA) levels of certain antioxidant genes in post-stroke patients given ELF-EMF therapy. Forty-eight post-stroke patients were divided into two groups: an ELF-EMF group and a non-ELF-EMF group. All patients underwent the same program of physical therapy, but the ELF-EMF group was additionally given ELF-EMF treatment...
November 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Tugba Kose, Mayra Vera-Aviles, Paul A Sharp, Gladys O Latunde-Dada
Ferroptosis is a form of programmed cell death that is characterized by lipid peroxidation and is inducible by iron and the accumulation of reactive oxygen species (ROS). It is triggered by erastin but inhibited by antioxidants such as -tocopherol, -carotene, polyphenols, and iron chelators such as deferoxamine (DFO), nitrilotriacetic acid (NTA), and ethylenediaminetetraacetic acid (EDTA). This study investigated the protective effects of two polyphenols, curcumin and (-)- epigallocatechin-3-gallate (EGCG), against iron loading and erastin-mediated ferroptosis in MIN6 cells...
February 6, 2019: Pharmaceuticals
Zheming Wu, Yang Geng, Xiaojuan Lu, Yuying Shi, Guowei Wu, Mengmeng Zhang, Bing Shan, Heling Pan, Junying Yuan
Necroptosis and ferroptosis are two distinct necrotic cell death modalities with no known common molecular mechanisms. Necroptosis is activated by ligands of death receptors such as tumor necrosis factor-α (TNF-α) under caspase-deficient conditions, whereas ferroptosis is mediated by the accumulation of lipid peroxides upon the depletion/or inhibition of glutathione peroxidase 4 (GPX4). The molecular mechanism that mediates the execution of ferroptosis remains unclear. In this study, we identified 2-amino-5-chloro-N,3-dimethylbenzamide (CDDO), a compound known to inhibit heat shock protein 90 (HSP90), as an inhibitor of necroptosis that could also inhibit ferroptosis...
February 4, 2019: Proceedings of the National Academy of Sciences of the United States of America
Yin He, Chun-Yu Liu, Chi-Chi He, Jin Zhao, Yan-Hui Sun, Hong-Shuai Xu, Xi-Qiang Cai, Yi-Fang Li, Kurihara Hiroshi, Rong-Rong He
The model of drug-induced liver injury (DILI) induced by acetaminophen (APAP) in mice was established to investigate the anti-oxidation and anti-ferroptosis mechanisms of Fuzheng Yanggan Mixture on DILI. C57BL/6 mice were randomly divided into five groups: control group, model group, positive group, and low and high-dose Fuzheng Yanggan Mixture groups (0.12, 0.24 g·kg⁻¹). Mice were intragastrically administration with Fuzheng Yanggan Mixture (0.12, 0.24 g·kg⁻¹) once per day for 21 consecutive days, and at the same time, mice were weighted every day...
December 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Francesco Marampon, Silvia Codenotti, Francesca Megiorni, Andrea Del Fattore, Simona Camero, Giovanni Luca Gravina, Claudio Festuccia, Daniela Musio, Francesca De Felice, Valerio Nardone, Anna Natalizia Santoro, Carlo Dominici, Alessandro Fanzani, Luigi Pirtoli, Antonella Fioravanti, Vincenzo Tombolini, Sara Cheleschi, Paolo Tini
PURPOSE: Tumor cells generally exhibit higher levels of reactive oxygen species (ROS), however, when stressed, tumor cells can undergo a process of 'Redox Resetting' to acquire a new redox balance with stronger antioxidant systems that enable cancer cells to become resistant to radiation therapy (RT). Here, we describe how RT affects the oxidant/antioxidant balance in human embryonal (RD) and alveolar (RH30) rhabdomyosarcoma (RMS) cell lines, investigating on the molecular mechanisms involved...
January 30, 2019: Journal of Cancer Research and Clinical Oncology
Lei Yang, Zhen Gao, Lanjie Lei, Qizhuang Lv, Qihan Zhao, Lixin Li, Xiaoming Cao, Wenxue Fu
Lycium barbarum polysaccharide (LBP) exhibits multiple pharmacological and biological effects, including displaying antioxidant and cytoprotective properties. The current study investigated the effects of LBP-supplemented culture medium on mitochondrial distribution, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) production, mitochondrial deoxyribonucleic acid (mtDNA) copy number, reactive oxygen species (ROS) accumulation, and development of previously-cryopreserved murine two-cell embryos...
January 31, 2019: Journal of Reproduction and Development
Lin Cong, Xiyu Dong, Yan Wang, Yulin Deng, Bo Li, Rongji Dai
In addition to amyloid cascade hypothesis, ferroptosis - a recently identified cell death pathway associated with the accumulation of lipid hydroperoxides - was hypothesized as one of the main forms of cell death in Alzheimer's disease. Herein, a series of hydroxylated chalcones were designed and synthesized as dual-functional inhibitors to inhibit amyloid-β peptide (Aβ) aggregation as well as ferroptosis simultaneously. Thioflavin-T assay indicated trihydroxy chalcones inhibited Aβ aggregation better. In human neuroblastoma SH-SY5Y cells, cytoprotective chalcones 14a-c with three hydroxyl substituents exhibited a significant neuroprotection against Aβ1-42 aggregation induced toxicity...
January 17, 2019: European Journal of Medicinal Chemistry
Zeeshan Ahmed Khan, Chinmoy Mishra, Tushar Jyotiranjan
Climatic change induced heat stress causes a formidable challenge for maintaining optimum productivity in goats. The SOD1 gene (superoxide dismutase 1) is one of the genes that regulates the heat tolerance capacity. This study aimed to in silico characterize this gene. Sequence based phylogenetic tree analysis showed the caprine SOD1 gene has close evolutional relationship with that of sheep. STRING database reveals its interaction with SOD2 (Superoxide dismutase [Mn], mitochondrial), ATOX1 (Copper transport protein), RAC1 (Ras-related C3 botulinum toxin substrate 1), GPX2 (Glutathione peroxidase 2), GPX4 (Glutathione peroxidase 4), GPX5, GPX6, CAT (Catalase)...
January 23, 2019: Genomics
Priscila Chediek Dall'Acqua, Giovana Barros Nunes, Cíntia Rodrigues da Silva, Patrícia Kubo Fontes, Marcelo Fábio Gouveia Nogueira, Flávia Lombardi Lopes, Márcia Marinho, Gisele Zoccal Mingoti
The epidermal growth factor receptor (EGFR) pathway is directly involved in oocyte meiotic resumption induced by a gonadotropic stimulus. Here, we used an EGFR inhibitor (AG1478) to inhibit spontaneous meiosis resumption in bovine oocytes (EGFR- group) during 8 hours prematuration and assessed the competence of such oocytes for embryonic development, apoptosis and gene expression in comparison to Control group which was not prematured. Data are presented as mean±SEM. Blastocysts rate on day 7 (40.81%, averaged) and hatching rate on day 9 (77...
January 18, 2019: Reproduction in Domestic Animals, Zuchthygiene
Bo-Jian Chen, Wen-Yi Zhang, Cui-Juan Niu, Wen-Jie Li, Hui Jia, Kenneth B Storey
The antioxidant defense protects turtles from oxidative stress caused by adverse environment conditions, such as acute thermal fluctuations. However, how these defenses work remains unclear. The present study examined changes in key enzymes of the enzymatic antioxidant system and the glutathione (GSH) system at both the mRNA and enzyme activity levels during acute cold exposure and following recovery in juvenile Chinese soft-shelled turtles, Pelodiscus sinensis Transcript levels of the upstream regulator NF-E2 related factor 2 (Nrf2) were also measured...
January 10, 2019: Journal of Experimental Biology
Jian Sun, Zixin Min, Wenxiang Zhao, Safdar Hussain, Yitong Zhao, Dongxian Guo, Fujun Zhang, Yuanxu Guo, Mengyao Sun, Huang Huang, Yan Han, Nannan Zhong, Peng Xu, Shemin Lu
OBJECTIVE: Both selenium (Se) deficiency and mycotoxin T2 lead to epiphyseal plate lesions, similar to Kashin-Beck disease (KBD). However, regulation of selenoproteins synthesis mediated by SECISBP2, in response to these 2 environmental factors, remained unclear. The present study proposed to explore the mechanism behind the cartilage degradation resulting from Se deficiency and mycotoxin T2 exposure. DESIGN: Deep chondrocyte necrosis and epiphyseal plate lesions were replicated in Dark Agouti (DA) rats by feeding them T2 toxin/Se deficiency artificial synthetic diet for 2 months...
December 29, 2018: Cartilage
Minghui Gao, Junmei Yi, Jiajun Zhu, Alexander M Minikes, Prashant Monian, Craig B Thompson, Xuejun Jiang
Ferroptosis is a regulated necrosis process driven by iron-dependent lipid peroxidation. Although ferroptosis and cellular metabolism interplay with one another, whether mitochondria are involved in ferroptosis is under debate. Here, we demonstrate that mitochondria play a crucial role in cysteine-deprivation-induced ferroptosis but not in that induced by inhibiting glutathione peroxidase-4 (GPX4), the most downstream component of the ferroptosis pathway. Mechanistically, cysteine deprivation leads to mitochondrial membrane potential hyperpolarization and lipid peroxide accumulation...
December 13, 2018: Molecular Cell
Ahmed Gad, Said Abu Hamed, Mohamed Khalifa, Ahmed Amin, Ashraf El-Sayed, Swiefy A Swiefy, Salah El-Assal
Retinoic acid, vitamin A metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. The objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisRA) on the quality and maturation rate of buffalo oocytes. Cumulus-oocyte complexes (COCs, n = 460) were collected from ovaries of slaughtered buffalos. Varying concentrations of 9-cisRA (0, 5, 50, and 200 nM) were added to the maturation medium, and the following parameters were analyzed: (i) maturation and cleavage rates, (ii) mitochondrial activity and reactive oxygen species (ROS) levels, (iii) expression level of antioxidant-related genes ( PRDX1, SOD1, CAT, HOMX1, and GPX4 ) using RT-qPCR...
December 2018: International Journal of Veterinary Science and Medicine
Xue Yao, Yan Zhang, Jian Hao, Hui-Quan Duan, Chen-Xi Zhao, Chao Sun, Bo Li, Bao-You Fan, Xu Wang, Wen-Xiang Li, Xuan-Hao Fu, Yong Hu, Chang Liu, Xiao-Hong Kong, Shi-Qing Feng
Ferroptosis is an iron-dependent novel cell death pathway. Deferoxamine, a ferroptosis inhibitor, has been reported to promote spinal cord injury repair. It has yet to be clarified whether ferroptosis inhibition represents the mechanism of action of Deferoxamine on spinal cord injury recovery. A rat model of Deferoxamine at thoracic 10 segment was established using a modified Allen's method. Ninety 8-week-old female Wistar rats were used. Rats in the Deferoxamine group were intraperitoneally injected with 100 mg/kg Deferoxamine 30 minutes before injury...
March 2019: Neural Regeneration Research
Guiqin Peng, Zongwei Tang, Yongjia Xiang, Wanyi Chen
Reactive oxygen species (ROS) have been widely accepted as critical molecules playing regulatory roles in various biological processes, including proliferation, differentiation and apoptotic/ferroptotic/necrotic cell death. Emerging evidence suggests that ROS may be involved in the induction of epithelial‑to‑mesenchymal transition (EMT), which has been reported to promote cancer stem‑like cell (CSC) generation. Recent data indicate that altered accumulation of ROS is associated with CSC generation, EMT and hypoxia exposure, but the underlying mechanisms are poorly understood...
December 6, 2018: Oncology Reports
Bing Huang, Jia-Jian Liang, Xi Zhuang, Shao-Wan Chen, Tsz Kin Ng, Haoyu Chen
Purpose: Oxidative stress is a common pathological condition for multiple retinal diseases. Hydrogen peroxide (H2 O2 ) has been applied as an oxidative stress inducer for the in vitro studies. Here, we report the in vivo effect of H2 O2 exposure to the mouse retina and its underlying mechanism. Methods: The H2 O2 or saline solution was intravitreally injected into the eyes of female C57BL/6J mice for two consecutive days. The retinal structure was evaluated by in vivo imaging using spectral domain optical coherence tomography (OCT) and validated by histological assessment as well as retinal marker expression...
2018: Oxidative Medicine and Cellular Longevity
Xinbing Sui, Ruonan Zhang, Shuiping Liu, Ting Duan, Lijuan Zhai, Mingming Zhang, Xuemeng Han, Yu Xiang, Xingxing Huang, Haoming Lin, Tian Xie
Ferroptosis is an iron-dependent, oxidative cell death, and is characterized by iron-dependent accumulation of reactive oxygen species (ROS) within the cell. It has been implicated in various human diseases, including cancer. Recently, ferroptosis, as a non-apoptotic form of cell death, is emerging in specific cancer types; however, its relevance in colorectal cancer (CRC) is unexplored and remains unclear. Here, we showed that ferroptosis inducer RSL3 initiated cell death and ROS accumulation in HCT116, LoVo, and HT29 CRC cells over a 24 h time course...
2018: Frontiers in Pharmacology
Hamed Alborzinia, Tatiana I Ignashkova, Francesca R Dejure, Mathieu Gendarme, Jannick Theobald, Stefan Wölfl, Ralph K Lindemann, Jan H Reiling
Cytotoxic activities of several Golgi-dispersing compounds including AMF-26/M-COPA, brefeldin A and golgicide A have previously been shown to induce autophagy or apoptosis. Here, we demonstrate that these Golgi disruptors also trigger ferroptosis, a non-apoptotic form of cell death characterized by iron-dependent oxidative degradation of lipids. Inhibitors of ferroptosis not only counteract cell death, but they also protect from Golgi dispersal and inhibition of protein secretion in response to several Golgi stress agents...
2018: Communications biology
Shuaifei Wang, Fangyuan Li, Ruirui Qiao, Xi Hu, Hongwei Liao, Lumin Chen, Jiahe Wu, Wu Haibin, Meng Zhao, Jianan Liu, Rui Chen, Xibo Ma, Dokyoon Kim, Jihong Sun, Thomas P Davis, Chunying Chen, Jie Tian, Taeghwan Hyeon, Daishun Ling
Ferroptosis, an iron-based cell death pathway, has recently attracted great attention owing to its effectiveness in killing cancer cells. Previous investigations focused on the development of iron-based nanomaterials to induce ferroptosis in cancer cells by the upregulation of reactive oxygen species (ROS) generated by the well-known Fenton reaction. Herein, we report a ferroptosis-inducing agent based on arginine-rich manganese silicate nanobubbles (AMSNs) that possess highly efficient glutathione (GSH) depletion ability and thereby induce ferroptosis by the inactivation of glutathione-dependent peroxidases 4 (GPX4)...
November 29, 2018: ACS Nano
Q Li, Y Jia, W R Burris, P J Bridges, J C Matthews
The goal of this study was to test the hypothesis that sodium selenite (ISe), SEL-PLEX (OSe), vs. a 1:1 blend (MIX) of ISe and OSe in a basal vitamin-mineral (VM) mix would differentially alter pituitary transcriptome profiles in growing beef steers grazing an endophyte-infected tall fescue (E+) pasture. Predominately-Angus steers (BW = 183 ± 34 kg) were randomly selected from fall-calving cows grazing E+ pasture and consuming VM mixes that contained 35 ppm Se as ISe, OSe, or MIX forms. Steers were weaned, depleted of Se for 98 d, and subjected to summer-long common grazing of a 10...
November 22, 2018: Journal of Animal Science
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