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https://read.qxmd.com/read/30955667/digestibility-in-the-gastrointestinal-tract-and-migration-to-blood-of-%C3%AE-component-onk-k-5-a-major-salmon-roe-ige-binding-protein
#1
Issei Kyosaka, Shingo Fujita, Yutaka Shimizu, Hiroki Saeki
The major allergen of chum salmon (Oncorhynchus keta) roe is the β'-component (Onc k 5, β'-c), which is a yolk protein and a fragment of vitellogenin. When yolk content containing β'-c was orally administered to mice, β'-c passed through the gastrointestinal tract and was excreted in feces without marked degradation. The direct administration of β'-c to ligated jejunal and ileal loops showed that β'-c was absorbed through the small intestine and transferred into the blood. Immunohistochemical staining showed that orally administered β'-c was distributed from the apical side to the basal side of intestinal epithelial cells, suggesting that endocytosis may be involved in the intestinal absorption of β'-c...
August 15, 2019: Food Chemistry
https://read.qxmd.com/read/30925937/unexpected-insertion-of-carrier-dna-sequences-into-the-fission-yeast-genome-during-crispr-cas9-mediated-gene-deletion
#2
Sophie Longmuir, Nabihah Akhtar, Stuart A MacNeill
OBJECTIVES: The fission yeast Schizosaccharomyces pombe is predicted to encode ~ 200 proteins of < 100 amino acids, including a number of previously uncharacterised proteins that are found conserved in related Schizosaccharomyces species only. To begin an investigation of the function of four of these so-called microproteins (designated Smp1-Smp4), CRISPR-Cas9 genome editing technology was used to delete the corresponding genes in haploid fission yeast cells. RESULTS: None of the four microprotein-encoding genes was essential for viability, meiosis or sporulation, and the deletion cells were no more sensitive to a range of cell stressors than wild-type, leaving the function of the proteins unresolved...
March 29, 2019: BMC Research Notes
https://read.qxmd.com/read/30897202/impact-of-preservation-of-the-latissimus-dorsi-muscle-through-a-left-anteroaxillary-thoracotomy-on-spinal-cord-protection-in-descending-thoracic-and-thoraco-abdominal-aortic-operations%C3%A2
#3
Toshiki Fujiyoshi, Toshiya Nishibe, Nobusato Koizumi, Masaki Kano, Shun Suzuki, Akinari Iwahori, Keta Maruno, Koji Kawago, Satoshi Takahashi, Toru Iwahashi, Kentarou Kamiya, Hitoshi Ogino
OBJECTIVES: The presence of collateral network circulation to the spinal cord, which is reflected in the repair of a descending thoracic aortic aneurysm (dAo) and a thoraco-abdominal aortic aneurysm (TAAo), has been demonstrated in clinical and animal experimental data. The latissimus dorsi muscle (LDM) including the thoracodorsal artery might be one of the major sources of this collateral network. The objective of this study was to evaluate the impact on spinal cord safety of a left anteroaxillary thoracotomy with minimal muscle division including preservation of the LDM in surgery for dAo and TAAo...
March 20, 2019: European Journal of Cardio-thoracic Surgery
https://read.qxmd.com/read/30766905/dataset-for-characterization-of-thrombospondin-family-in-chum-salmon-oncorhynchus-keta
#4
Sang Yoon Lee, Yi Kyung Kim
An extracellular matrix (ECM) is composed of multiprotein networks for stable interactions between cells. Thrombospondins (TSPs) are known to exert extracellular matrix interactions, synapse formation, angiogenesis and immune response in vertebrates. A five TSP gene family is divided into two subfamilies on basis of their domain architecture. Until recently, exploitation of diverse TSP genes in teleost has been still limitedly exemplified. Therefore, we report the cDNA structures and expression profiles for TSPs-1, 2, 3A, 3B, and 4B of chum salmon, Oncorhynchus keta ...
February 2019: Data in Brief
https://read.qxmd.com/read/30740943/a-comparison-of-the-remineralizing-potential-of-dental-restorative-materials-by-analyzing-their-fluoride-release-profiles
#5
Alicja Porenczuk, Bartłomiej Jankiewicz, Magdalena Naurecka, Bartosz Bartosewicz, Bartosz Sierakowski, Dariusz Gozdowski, Jerzy Kostecki, Barbara Nasiłowska, Agnieszka Mielczarek
BACKGROUND: The accessibility of the remineralizing ions in teeth's environment is essential for their incorporation into caries-affected dentin. Novel bioglass-reinforced materials capable of releasing fluoride, calcium and phosphates may be particularly useful in the tissue remineralization process. A novel restorative material, ACTIVA BioActive-Restorative (Pulpdent Corp., Watertown, USA), is a hydrophilic resin-modified glassionomer cement (RMGIC) enriched with bioglass particles and fortified with a patented rubberized polymer resin...
February 8, 2019: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://read.qxmd.com/read/30630968/chum-salmon-migrating-upriver-accommodate-to-environmental-temperatures-through-metabolic-compensation
#6
Takaaki K Abe, Takashi Kitagawa, Yuya Makiguchi, Katsufumi Sato
Ectotherms adjust their thermal performances to various thermal ranges by altering their metabolic rates. These metabolic adjustments involve plastic and/or genetic traits and pathways depend on species-specific ecological contexts. Chum salmon ( Oncorhynchus keta Walbaum) are ecologically unique among the Pacific salmonids, as early-run and late-run populations are commonly observed in every part of their range. In the Sanriku coastal area, Japan, early-run adults experience high water temperatures (12-24°C) during their migration, compared with the late-run adults (4-15°C), suggesting that the two populations might have different thermal performances...
January 10, 2019: Journal of Experimental Biology
https://read.qxmd.com/read/30611815/evaluation-of-growth-status-using-endocrine-growth-indices-insulin-like-growth-factor-igf-i-and-igf-binding-protein-1b-in-out-migrating-juvenile-chum-salmon
#7
Nobuto Kaneko, Mitsuru Torao, Yousuke Koshino, Makoto Fujiwara, Yasuyuki Miyakoshi, Munetaka Shimizu
This study aimed to utilize circulating insulin-like growth factor binding protein (IGFBP)-1b as a negative index of growth to evaluate the growth status of juvenile chum salmon (Oncorhynchus keta) in the ocean. First, rearing experiments using PIT-tagged juveniles were conducted to examine the relationship of circulating IGFBP-1b with growth rate of the fish in May and in June. The serum IGFBP-1b level negatively correlated with fish growth rate in both months, suggesting its utility as a negative index of growth...
January 3, 2019: General and Comparative Endocrinology
https://read.qxmd.com/read/30585881/detection-of-an-intraoperative-cerebral-event-with-entropy-monitoring
#8
Nitin Manohar, Keta Thakkar, Astha Palan, Kartik M Multani
No abstract text is available yet for this article.
December 21, 2018: Journal of Neurosurgical Anesthesiology
https://read.qxmd.com/read/30487280/closing-and-opening-holes-in-the-glycan-shield-of-hiv-1-envelope-glycoprotein-sosip-trimers-can-redirect-the-neutralizing-antibody-response-to-the-newly-unmasked-epitopes
#9
Rajesh P Ringe, Pavel Pugach, Christopher A Cottrell, Celia C LaBranche, Gemma E Seabright, Thomas J Ketas, Gabriel Ozorowski, Sonu Kumar, Anna Schorcht, Marit J van Gils, Max Crispin, David C Montefiori, Ian A Wilson, Andrew B Ward, Rogier W Sanders, P J Klasse, John P Moore
In HIV-1 vaccine research, native-like, soluble envelope glycoprotein SOSIP trimers are widely used for immunizing animals. The epitopes of autologous neutralizing antibodies (NAbs) induced by the BG505 and B41 SOSIP trimers in rabbits and macaques have been mapped to a few holes in the glycan shields that cover most of the protein surfaces. For BG505 trimers, the dominant autologous NAb epitope in rabbits involves residues that line a cavity caused by the absence of a glycan at residue 241. Here, we blocked this epitope in BG505 SOSIPv4...
November 28, 2018: Journal of Virology
https://read.qxmd.com/read/30451568/biological-outcomes-of-%C3%AE-radiation-induced-dna-damages-in-breast-and-lung-cancer-cells-pretreated-with-free-radical-scavengers
#10
Vladana D Petković, Otilija D Keta, Marija Z Vidosavljević, Sebastien Incerti, Aleksandra M Ristić Fira, Ivan M Petrović
PURPOSE: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high LET radiations used in hadron therapy, i.e., protons and carbon ions. MATERIALS AND METHODS: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers...
November 19, 2018: International Journal of Radiation Biology
https://read.qxmd.com/read/30397926/experimental-evidence-on-prevention-of-infection-by-the-ectoparasitic-protozoans-ichthyobodo-salmonis-and-trichodina-truttae-in-juvenile-chum-salmon-using-ultraviolet-disinfection-of-rearing-water
#11
Shinya Mizuno, Shigehiko Urawa, Mahito Miyamoto, Makoto Hatakeyama, Nobuhisa Koide, Hiroshi Ueda
In northern Japan, juvenile chum salmon Oncorhynchus keta (Walbaum) are released from hatcheries to enhance the fishery resource. Infections with ectoparasitic protozoans, particularly the flagellate Ichthyobodo salmonis and the ciliate Trichodina truttae, occasionally cause severe mortality among hatchery-reared juveniles. This study examined the susceptibility of the two parasites to wide-ranging UV irradiation (experiment 1) and then investigated whether UV disinfection of the rearing water using a commercial device was useful for preventing infections among juveniles in a small-scale rearing system over a 28-day period (experiment 2)...
November 5, 2018: Journal of Fish Diseases
https://read.qxmd.com/read/30320361/an-effective-range-of-polydeoxyribonucleotides-is-critical-for-wound-healing-quality
#12
Kyu-Hee Hwang, Ji-Hee Kim, Eun Young Park, Seung-Kuy Cha
Wound healing is a physiological restorative response to tissue and cell injury. This process occurs in collaboration with a complex cascade of cellular events, including biochemical alterations to the extracellular matrix. Polydeoxyribonucleotide (PDRN) is a fragmented DNA mixture from Oncorhynchus mykiss or Oncorhynchus keta sperm known to promote tissue regeneration under different pathophysiological conditions. However, the most effective molecular size of PDRNs for promoting the wound healing process and quality has not been established...
December 2018: Molecular Medicine Reports
https://read.qxmd.com/read/30283873/it-works-but-for-whom-examining-racial-bias-in-carding-experiences-and-acceptance-of-a-county-identification-card
#13
Alana M W LeBrón, Keta Cowan, William D Lopez, Nicole L Novak, Maria Ibarra-Frayre, Jorge Delva
Purpose: Policies that restrict access to U.S. government-issued photo identification (ID) cards adversely affect multiple marginalized communities. This context impedes access to health-promoting resources that increasingly require government-issued IDs and exacerbates health inequities. In 2015, Washtenaw County, Michigan, implemented the Washtenaw ID to improve access to resources contingent upon having an ID. We employed an audit study to examine whether Washtenaw ID users experienced racially biased treatment in carding experiences and acceptance of the Washtenaw ID...
2018: Health Equity
https://read.qxmd.com/read/30236910/molecular-characterization-and-gene-expression-of-syntaxin-1-and-vamp2-in-the-olfactory-organ-and-brain-during-both-seaward-and-homeward-migrations-of-chum-salmon-oncorhynchus-keta
#14
Takashi Abe, Hideaki Kudo
Anadromous Pacific salmon (Genus Oncorhynchus) imprint odorants from their natal streams during their seaward migration, and adult salmon use olfaction to identify their natal streams during their homeward migration. However, little is known about the molecular mechanisms of olfactory imprinting in the salmon nervous system. Our previous study suggested that the snap25s gene (encoding a soluble N-ethylmaleimide-sensitive factor attachment protein receptor [SNARE] protein) is involved in pre-synaptic functions for olfactory imprinting and/or olfactory memory retrieval in chum salmon (O...
September 17, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://read.qxmd.com/read/30148220/detection-and-localization-of-the-thyroid-hormone-receptor-beta-mrna-in-the-immature-olfactory-receptor-neurons-of-chum-salmon
#15
Hideaki Kudo, Akihiro Eto, Takashi Abe, Kazuhiko Mochida
Thyroid hormone (TH) plays an important role in regulating multiple cellular and metabolic processes, including cell proliferation, cell death, and energy metabolism, in various organs and tissues of vertebrates. It is generally accepted that anadromous Pacific salmon ( Oncorhynchus spp.) imprint odorants from their natal stream during their seaward migration, and they then use olfaction to discriminate their natal stream during the spawning migration. Both serum TH levels and the specific binding values of TH in the salmon olfactory epithelium were markedly increased during the seaward migration...
August 2018: Heliyon
https://read.qxmd.com/read/30103118/quantitation-of-residual-host-cell-dna-in-protaminesulfate-drug-product-by-qpcr
#16
Cynthia Sommers, Barry Rosenzweig, Lida Oum, Karol Thompson, David A Keire
Protamine sulfate (PS) is an FDA approved drug used to reverse heparin-induced anticoagulation in patients. Protamine sulfate is a mixture of primarily four ∼4 kDa arginine-rich cationic polypeptide chains derived from chum (Oncorhynchus keta) salmon sperm. Because the presence of residual host cell salmon DNA (resDNA) in PS drug product can pose safety concerns, processing steps during PS manufacturing are designed to target the reduction of these impurities. However, given protamine's positively charged structure, isolating and measuring negatively charged residual DNA is challenging...
October 25, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://read.qxmd.com/read/29938795/effects-of-dietary-supplementation-with-oregano-essential-oil-on-prevention-of-the-ectoparasitic-protozoans-ichthyobodo-salmonis-and-trichodina-truttae-in-juvenile-chum-salmon-oncorhynchus-keta
#17
S Mizuno, S Urawa, M Miyamoto, M Hatakeyama, Y Sasaki, N Koide, S Tada, H Ueda
The present study performed three experiments to establish a practical prevention strategy for the ectoparasitic flagellate Ichthyobodo salmonis and ciliate Trichodina truttae in hatchery-reared juvenile chum salmon Oncorhynchus keta using dietary supplementation with oregano essential oil. Experiment 1 showed that a diet supplemented for 3 weeks with 0.02% oregano essential oil significantly prevented infection with I. salmonis and T. truttae in juveniles reared in small tanks. Experiment 2, in outdoor hatchery ponds, demonstrated that the oregano treatment completely prevented I...
June 25, 2018: Journal of Fish Biology
https://read.qxmd.com/read/29669838/effects-of-adjuvants-on-hiv-1-envelope-glycoprotein-sosip-trimers-in-vitro
#18
Gabriel Ozorowski, Albert Cupo, Michael Golabek, Michelle LoPiccolo, Thomas A Ketas, Matt Cavallary, Christopher A Cottrell, P J Klasse, Andrew B Ward, John P Moore
Native-like, soluble, recombinant SOSIP trimers of various designs and based on several env genes of human immunodeficiency virus type 1 (HIV-1) are being tested as immunogens in different animal models. These experiments almost always involve coformulating the trimers with an adjuvant to boost the magnitude of the immune responses. One factor relevant to the choice of an adjuvant is that it should not physically damage the immunogen or impede its ability to present relevant epitopes. As examples, an adjuvant formulation that includes harsh detergents could disrupt the structural integrity of a trimer, and any charged compounds in the formulation could bind to countercharged regions of the trimer and physically occlude nearby epitopes...
July 1, 2018: Journal of Virology
https://read.qxmd.com/read/29609429/comparison-of-wound-healing-effects-between-oncorhynchus-keta-derived-polydeoxyribonucleotide-pdrn-and-oncorhynchus-mykiss-derived-pdrn
#19
Jong Hun Lee, Jin Woo Han, Jin Hwan Byun, Won Mi Lee, Min Ho Kim, Wen Hao Wu
BACKGROUND: Polydeoxyribonucleotide (PDRN) influencing cellular growth and differentiation is recognized to promote wound healing by stimulating tissue repair. Although PDRN can be extracted from human placentas, PDRN medications have recently been extracted from the semen of trout ( Oncorhynchus mykiss ) and salmon ( Oncorhynchus keta ). The present study was designed to evaluate the wound healing effects of O. keta -derived PDRN for injection (Rejuvenex) and PDRN cream (Rejuvenex Cream) in comparison with those of O...
March 2018: Archives of Craniofacial Surgery
https://read.qxmd.com/read/29554567/interspecies-variation-in-the-susceptibility-of-adult-pacific-salmon-to-toxic-urban-stormwater-runoff
#20
Jenifer K McIntyre, Jessica I Lundin, James R Cameron, Michelle I Chow, Jay W Davis, John P Incardona, Nathaniel L Scholz
Adult coho salmon (Oncorhynchus kisutch) prematurely die when they return from the ocean to spawn in urban watersheds throughout northwestern North America. The available evidence suggests the annual mortality events are caused by toxic stormwater runoff. The underlying pathophysiology of the urban spawner mortality syndrome is not known, and it is unclear whether closely related species of Pacific salmon are similarly at risk. The present study co-exposed adult coho and chum (O. keta) salmon to runoff from a high traffic volume urban arterial roadway...
July 2018: Environmental Pollution
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