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Rim Serhal, Nagib Saliba, George Hilal, Mayssam Moussa, Ghada S Hassan, Oula El Atat, Nada Alaaeddine
AIM: To investigate the effect of adipose-derived mesenchymal stem cells (ADMSCs) and their conditioned media (CM) on hepatocellular carcinoma (HCC) cell tumorigenesis. METHODS: The proliferation rate of HepG2 and PLC-PRF-5 HCC cancer cells was measured using the trypan blue exclusion method and confirmed using the cell-counting kit 8 (commonly known as CCK-8) assay. Apoptosis was detected by flow cytometry using annexin V-FITC. Protein and mRNA expression was quantified by ELISA and real time PCR, respectively...
February 7, 2019: World Journal of Gastroenterology: WJG
Jae Han Ko, Young Mi Kang, Jae Ho Yang, Ji Sup Kim, Won Jai Lee, Sang Ho Kim, Ick Hwan Yang, Seong Hwan Moon
PURPOSE: The aim of this study was to investigate the effects of relaxin (RLN) expression on fibrosis inhibition in synovial fibroblasts. MATERIALS AND METHODS: Tissue cells from patients with knee osteoarthritis and >30° flexion contractures were utilised. Synovial fibroblasts were activated by TGF-β1 (two nanograms per millilitre) and then exposed to Ad-RLN as a therapeutic gene, adenovirus-lacZ construct as a marker gene, and SB505124 as an inhibitor for TGF-β1 signal for 48 h...
February 13, 2019: Knee
Felix Nagel, David Santer, Stefan Stojkovic, Christoph Kaun, Anne-Kristin Schaefer, Martin Kššák, Dietmar Abraham, Péter Bencsik, Péter Ferdinandy, Eva Kenyeres, Tamara Szabados, Johann Wojta, Karola Trescher, Attila Kiss, Bruno K Podesser
The aim of this study was to describe the potential associations of the expression of matricellular components in adverse post-infarction remodeling of the geriatric heart. In male geriatric (OM, age: 18 months) and young (YM, age: 11 weeks) OF1 mice myocardial infarction (MI) was induced by permanent ligation of the left anterior descending coronary artery. Cardiac function was evaluated by MRI. Plasma and myocardial tissue samples were collected 3d, 7d, and 32d post-MI. Age and MI were associated with impaired cardiac function accompanied by left-ventricular (LV) dilatation...
February 11, 2019: Experimental Gerontology
Diego Pulzatto Cury, Bárbara Tavares Schäfer, Sonia Regina Yokomizo de Almeida, Marta Maria da Silva Righetti, Ii-Sei Watanabe
BACKGROUND: The prolonged tendon-healing process, the high costs associated with treatment, the increase in the number of injuries over the past decades, and the lack of consensus on the optimal treatment of tendon injuries are a global problem. Restoring the normal tendon anatomy and decreasing the healing time are key factors for treatment advancement. HYPOTHESIS: Application of a purified protein from natural latex (PPNL) accelerates the healing process, increasing collagen synthesis and decreasing metalloproteinases...
February 13, 2019: American Journal of Sports Medicine
Yifan Joshua Li, Katalin A Wilkinson, Robert J Wilkinson, Anthony A Figaji, Ursula K Rohlwink
We collected lumbar and ventricular cerebrospinal fluid and serum from 40 children treated for tuberculous meningitis and measured the concentrations of gelatinases and their inhibitors. The concentrations of matrix metalloproteinase 9 (MMP-9), MMP-2, tissue inhibitor of metalloproteinase 1 (TIMP-1), and TIMP-2 were significantly elevated in the lumbar CSF samples, and we found interesting dynamics for MMP-9 that offer novel insight into its role in pediatric patients with tuberculous meningitis.
February 12, 2019: Journal of the Pediatric Infectious Diseases Society
Sina Waldherr, Alexander Fichtner, Bernd Beedgen, Thomas Bruckner, Franz Schaefer, Burkhard Tönshoff, Johannes Pöschl, Timm H Westhoff, Jens H Westhoff
BACKGROUND: Serum creatinine (SCr)- or urine output-based definitions of acute kidney injury (AKI) have important limitations in neonates. This study evaluates the diagnostic value of urinary biomarkers in very low-birth-weight (VLBW) infants receiving indomethacin for closure of a patent ductus arteriosus (PDA). METHODS: Prospective cohort study in 14 indomethacin-treated VLBW infants and 18 VLBW infants without indomethacin as controls. Urinary biomarkers were measured before, during, and after indomethacin administration...
February 11, 2019: Pediatric Research
Philippe Grieshaber, Sina Möller, Borros Arneth, Peter Roth, Bernd Niemann, Harald Renz, Andreas Böning
BACKGROUND:  Prediction, early diagnosis, and therapy of cardiac surgery-associated acute kidney injury (CSA-AKI) are challenging. We prospectively tested a staged approach to identify patients at high risk for CSA-AKI combining clinical risk stratification and early postoperative quantification of urinary biomarkers for AKI. METHODS:  All patients, excluding those on chronic hemodialysis, undergoing scheduled surgery with cardiopulmonary bypass between August 2015 and July 2016 were included...
February 11, 2019: Thoracic and Cardiovascular Surgeon
Liyao Lin, Keluo Cheng, Zhan He, Qiongyan Lin, Yidong Huang, Chunyuan Chen, Zhanqiang Xie, Lin Chen, Zhu Liang
In this study we investigated the potential effects of a polysaccharide (HDP) from Hedyotis diffusa on the metastasis in human lung adenocarcinoma A549 cells. HDP (25, 50 and 100 μg/ml) significantly suppressed the cell adhesion, invasion and migration of A549 cells in a dose dependent manner by downregulation of matrix metalloproteinase (MMP-2 and MMP-9) and upregulation of tissue inhibitors of metalloproteinase (TIMP-2 and TIMP-9). Moreover, HDP effectively downregulated the protein expressions of epithelial-mesenchymal transition (EMT) markers (N-cadherin and vimentin), and upregulated E-cadherin protein expression, which is involved in interrupting EGFR/Akt/ERK signaling pathways, as well as inhibiting COX-2 protein expression...
February 7, 2019: International Journal of Biological Macromolecules
Marta Łukaszewicz-Zając, Mariusz Gryko, Sara Pączek, Maciej Szmitkowski, Bogusław Kędra, Barbara Mroczko
Objectives: The incidence rate of pancreatic cancer (PC) is similar to mortality rate, thus searching specific tumor biomarkers of PC is sorely needed. Matrix metalloproteinase-2 (MMP-2) and the imbalance between MMP-2 and its tissue inhibitor (TIMP-2) play a critical role in tumor progression. We aim to assess the diagnostic and prognostic usefulness of serum MMP-2 and TIMP-2 as potential biomarkers in comparison to well-established tumor markers of PC (CA 19-9, carbohydrate antigen 19-9 and CEA, carcinoembryonic antigen)...
January 8, 2019: Oncotarget
J Dinesh Kumar, Iman Aolymat, Laszlo Tiszlavicz, Zita Reisz, Hanan M Garalla, Rob Beynon, Deborah Simpson, Graham J Dockray, Andrea Varro
The chemokine-like peptide, chemerin, stimulates chemotaxis in several cell types. In this study we examined the expression of putative chemerin receptors in gastric cancer and the action of chemerin on cancer cell migration and invasion. Immunohistochemical studies of gastric tumors identified expression of two putative receptors, chemokine-like receptor-1 (CMKLR1) and G-protein coupled receptor 1(GPR1), in cancer cells; there was also some expression in stromal myofibroblasts although generally at a lower intensity...
January 4, 2019: Oncotarget
Yaqin Jiang, Canwei Zhang, Jianli Ma, Luping Wang, Jing Gao, Jiantao Ren, Wei He, Sheng Wang, Shuai Sheng, Xudong Huang
BACKGROUND: To investigate the expression of Matrix Metalloproteinases 2 and aquaporin-1 in corneoscleral junction and explore the mechanism of trabecular damageafter angle-closure. METHODS: Thirty New Zealand white rabbits were randomly assigned into 2 groups, theexperimental group (Group 1) including twenty five rabbits and the control group (Group 2) including 5 rabbits. The rabbits in the experimental group were used to establish angle-closure models, and the rabbits in the control group were not subjected to any operation...
February 4, 2019: BMC Ophthalmology
Yuanjian Chen, Wenyuan Zhao, Chang Liu, Weixin Meng, Tieqiang Zhao, Syamal K Bhattacharya, Yao Sun
Background: Studies implicate that Ang1-7 imparts protective effects in the kidney. However, its relevance in hypertensive kidney disease is not fully understood. The purpose of this study is to explore the role of Ang1-7 on renal damage/remodeling during hypertension and its potential underlying molecular-cellular mechanisms. Methods: Hypertension was induced in adult Sprague Dawley rats by infusion of ALDO (0.75μg/h) for 4 weeks with or without co-treatment of Ang1-7 (1mg/kg/day)...
January 29, 2019: American Journal of Hypertension
Larissa Berloffa Belardin, Mariana Pereira Antoniassi, Mariana Camargo, Paula Intasqui, Renato Fraietta, Ricardo Pimenta Bertolla
Matrix Metalloproteinases (MMPs) and their regulators - Tissue Inhibitors of Matrix Metalloproteinases (TIMPs) - participate in extracellular matrix remodeling, fibrosis, and semen liquefaction, as well as to inflammatory activity. Seminal plasma has been shown to contain MMPs (MMP-2 and MMP-9) and TIMPs (TIMP-1 and TIMP-2). Also, a link between MMPs gene expression and excessive reactive oxygen species (ROS) has been established. In semen, ROS are associated with altered sperm function and increased DNA fragmentation...
January 29, 2019: Scientific Reports
Stephanie L C Scofield, Suman Dalal, Kristina A Lim, Patsy R Thrasher, Christopher R Daniels, Jonathan M Peterson, Mahipal Singh, Krishna Singh
β-adrenergic receptor (β-AR) stimulation increases extracellular levels of ubiquitin (UB) in myocytes, and exogenous UB decreases β-AR-stimulated myocyte apoptosis and myocardial fibrosis. Here, we hypothesized that exogenous UB modulates inflammatory response, thereby playing a protective role in cardiac remodeling following ischemia/reperfusion (I/R) injury. C57Bl/6 mice, infused with vehicle or UB (1mg/g/h), were subjected to myocardial I/R injury. Functional and biochemical parameters of the heart were examined 3 days post-I/R...
January 25, 2019: American Journal of Physiology. Heart and Circulatory Physiology
Xin Yao, Wei Jiang, Danhong Yu, Zhaowei Yan
Since the incidence rate of malignant melanoma is increasing annually, development of drugs against melanoma cell metastasis has become more urgent. Luteolin, a naturally occurring flavonoid, is abundant in our daily dietary intake and exhibits a wide spectrum of pharmacological properties. However, the potential anti-cancer role of luteolin in melanoma cells has not been fully investigated. In this study, we have explored whether luteolin inhibits the migration and invasion of A375 human melanoma cells and further elucidated the underlying anti-cancer molecular mechanism of luteolin in melanoma cells...
January 21, 2019: Food & Function
Katleen Frauz, Luis Felipe R Teodoro, Giane Daniela Carneiro, Fernanda Cristina da Veiga, Danilo Lopes Ferrucci, André Luis Bombeiro, Priscyla Waleska Simões, Lúcia Elvira Álvares, Alexandre Leite R de Oliveira, Cristina Pontes Vicente, Rui Seabra Ferreira, Benedito Barraviera, Maria Esméria C do Amaral, Marcelo Augusto M Esquisatto, Benedicto de Campos Vidal, Edson Rosa Pimentel, Andrea Aparecida de Aro
Tissue engineering and cell-based therapy combine techniques that create biocompatible materials for cell survival, which can improve tendon repair. This study seeks to use a new fibrin sealant (FS) derived from the venom of Crotalus durissus terrificus , a biodegradable three-dimensional scaffolding produced from animal components only, associated with adipose-derived stem cells (ASC) for application in tendons injuries, considered a common and serious orthopedic problem. Lewis rats had tendons distributed in five groups: normal (N), transected (T), transected and FS (FS) or ASC (ASC) or with FS and ASC (FS + ASC)...
January 16, 2019: Cells
Ling-Yuh Shyu, Ke-Min Chen, Shih-Chan Lai
In ocular toxocariasis, Toxocara canis-induced inflammatory reaction can lead to eye destruction and granuloma, which is formed by immune cell infiltration and concurrent extensive remodeling tissue. Herein, the histomorphology of granuloma and proteinase production in the eye of T. canis-infected BALB/c mice were investigated. Pathological effects substantially increased after the infection culminated in a severe leukocyte infiltration and granuloma formation from days 4 to 56 post-inoculation. The matrix metalloproteinase (MMP)-2 and MMP-9 activities remarkably increased, compared with those of uninfected control, by gelatin zymography and Western blot analysis in ocular toxocariasis...
January 10, 2019: Parasitology Research
N E Kushlinskii, E S Gershtein, A A Ivannikov, M M Davydov, V L Chang, N A Ognerubov, I S Stilidi
Plasma levels of MMP-2, MMP-7, and MMP-9 and their tissue inhibitor TIMP-2 were measured in 89 patients with gastric cancer and the relationship between these parameters and the main clinical morphological characteristics of the disease was analyzed. Plasma levels of the proteins were measured using standard direct ELISA kits. The level of MMP-7 in patients with gastric cancer was significantly higher than in the control group (medians 2.7 and 1.2 ng/ml, respectively; p<0.01), but only in 51% patients this parameter surpassed the upper threshold normal value (2...
January 9, 2019: Bulletin of Experimental Biology and Medicine
Fudong Fan, Qing Zhou, Zhenjun Xu, Dongjin Wang
The pathogenesis of aortic dissection (AD) is unclear. The aim of this study was to explore the relationship between osteopontin (OPN) and AD. Fifty AD patients were enrolled; 29 had hypertension with AD (H-AD) and 21 no hypertension with HD (NH-AD). Twenty-five healthy controls (NH-C) and 14 patients with hypertension (H-C) were also enrolled. Serum and aortic wall OPN levels were determined. Human vascular muscle cells (HVSMC) were stimulated by both low (1 μg/mL) and high (5 μg/mL) concentrations of OPN and cell proliferation as well as apoptosis was measured...
January 10, 2019: International Heart Journal
Ilaria Saltarella, Fortunato Morabito, Nicola Giuliani, Carolina Terragna, Paola Omedè, Antonio Palumbo, Sara Bringhen, Lorenzo De Paoli, Enrica Martino, Alessandra Larocca, Massimo Offidani, Francesca Patriarca, Chiara Nozzoli, Tommasina Guglielmelli, Giulia Benevolo, Vincenzo Callea, Luca Baldini, Mariella Grasso, Giovanna Leonardi, Manuela Rizzo, Antonietta Pia Falcone, Daniela Gottardi, Vittorio Montefusco, Pellegrino Musto, Maria Teresa Petrucci, Franco Dammacco, Mario Boccadoro, Angelo Vacca, Roberto Ria
BACKGROUND: Several new drugs are approved for treatment of patients with multiple myeloma (MM), but no validated biomarkers are available for the prediction of a clinical outcome. We aimed to establish whether pretreatment blood and bone marrow plasma concentrations of major cytokines and angiogenic factors (CAFs) of patients from a phase 3 trial of a MM treatment could have a prognostic and predictive value in terms of response to therapy and progression-free and overall survival and whether these patients could be stratified for their prognosis...
January 9, 2019: Journal of Hematology & Oncology
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