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José Francisco Aguilar Guevara, Seila Lacarra Fernández, Oliver Rojas Claros, Pedro Giral Villalta, José Luis Cebrián Lostal, Miguel Angel Resano Abarzuza
Kaposi sarcoma is an angioproliferative disorder that ranges from a single indolent skin lesion to respiratory and gastrointestinal/visceral involvement. Kaposi sarcoma is rare in non-immunosuppressed patients. Nineteen cases of penile Kaposi sarcoma in HIV-negative patients were reported in 2012. We present the case report of a 48-year-old male patient with no previous medical history, who came to our urology clinic presenting a purple-color papule on the penis glans. Lab tests revealed negative serology for HIV, but tissue PCR was positive for human herpesvirus 8...
February 18, 2019: Einstein
Yuliang Cao, Matthew Li, Jun Lu, Jun Liu, Khalil Amine
Batteries have shaped much of our modern world. This success is the result of intense collaboration between academia and industry over the past several decades, culminating with the advent of and improvements in rechargeable lithium-ion batteries. As applications become more demanding, there is the risk that stunted growth in the performance of commercial batteries will slow the adoption of important technologies such as electric vehicles. Yet the scientific literature includes many reports describing material designs with allegedly superior performance...
February 18, 2019: Nature Nanotechnology
John R Richards, Gagan D Singh, Aman K Parikh, Sandhya Venugopal
Excluding ethanol, cannabis is the most commonly used drug in the United States and worldwide. Several published case series and reports have demonstrated an association between cannabis use and acute coronary syndrome (ACS). We report the first ever published case of ACS precipitated by cannabis use that was confirmed with concomitant rising quantitative plasma levels of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol, a secondary metabolite of cannabis. A 63-year-old non-tobacco smoking male with no prior medical history presented to the emergency department with chest pain immediately after smoking cannabis, and anterior ST-segment elevation pattern was observed on his electrocardiogram...
February 13, 2019: American Journal of Emergency Medicine
Lauren E Shipp, Rose Z Hill, Amro Hamdoun
Sea urchin gametes have been historically used to demonstrate fertilization and early development in student laboratories. Large amounts of egg and sperm are easily acquired, and the conspicuous changes in egg surface morphology, indicative of sperm-egg fusion and egg activation, are readily observed in the classroom. However, less often incorporated into teaching labs are exercises that demonstrate the dramatic metabolic changes that accompany egg activation. One example is the massive up-regulation of various essential transport activities in the embryo's plasma membrane, including xenobiotic transporter activity...
2019: Methods in Cell Biology
F Lehmann, J Rau, B Malcolm, M Sander, C von Heymann, T Moormann, T Geyer, F Balzer, K D Wernecke, L Kaufner
BACKGROUND: Adult cardiac surgery is often complicated by elevated blood losses that account for elevated transfusion requirements. Perioperative bleeding and transfusion of blood products are major risk factors for morbidity and mortality. Timely diagnostic and goal-directed therapies aim at the reduction of bleeding and need for allogeneic transfusions. METHODS: Single-centre, prospective, randomized trial assessing blood loss and transfusion requirements of 26 adult patients undergoing elective cardiac surgery at high risk for perioperative bleeding...
February 18, 2019: BMC Anesthesiology
Florian Laubé, Andy Poupon, Philippe Zinck, Christel Müller-Goymann, Stephan Reichl, Véronique Nardello-Rataj
The human nail, like any biological material, is not readily available in large amounts and shows some variability from one individual to another. Replacing it by synthetic models is of great interest to perform reproducible and reliable tests in order to assess drug diffusion or nail lacquer adhesion for example. Keratin films, produced at the lab scale from natural hair, and the commercially available Vitro-nail® sheets have been proposed as models of human nails. In this study, we have investigated in detail these two materials...
February 13, 2019: European Journal of Pharmaceutical Sciences
Mostafa Karimi, Di Wu, Zhangyang Wang, Yang Shen
MOTIVATION: Drug discovery demands rapid quantification of compound-protein interaction (CPI). However, there is a lack of methods that can predict compound-protein affinity from sequences alone with high applicability, accuracy, and interpretability. RESULTS: We present a seamless integration of domain knowledges and learning-based approaches. Under novel representations of structurally-annotated protein sequences, a semi-supervised deep learning model that unifies recurrent and convolutional neural networks has been proposed to exploit both unlabeled and labeled data, for jointly encoding molecular representations and predicting affinities...
February 2, 2019: Bioinformatics
Ek Raj Thapaliya, Jun Zhao, Graham C R Ellis-Davies
Azobenzenes are the most widely studied photoswitches, and have become popular optical probes for biological systems. The cis configuration is normally metastable, meaning the trans configuration is always thermodynamically favored. The unique exception to this rule is an azobenzene having a two-carbon bridge between ortho positions, substitutions that lock the photoswitch in its cis configuration. Only thoroughly chemically characterized relatively recently, we describe the first applications of this locked-azobenzene (or "LAB") scaffold with two derivatives designed to control ion flow in pyramidal neurons in acutely isolated brain slices...
February 15, 2019: ACS Chemical Neuroscience
Maria Cutumisu, Siddhi D Patel, Matthew R G Brown, Caroline Fray, Patrick von Hauff, Thomas Jeffery, Georg M Schmölzer
Background: The current resuscitation guidelines recommend frequent simulation based medical education (SBME). However, the current SBME approach is expensive, time-intensive, and requires a specialized lab and trained instructors. Hence, it is not offered routinely at all hospitals. We designed the board game "RETAIN" to train healthcare providers (HCPs) in neonatal resuscitation in a cost-friendly and accessible way. Objectives: To examine if a board game-based training simulator improves knowledge retention in HCPs...
2019: Frontiers in Pediatrics
Andrzej Białowiec, Jacek A Koziel, Piotr Manczarski
In this research, we explore for the first time the use of leaf stomatal conductance (gs ) for phytotoxicity assessment. Plants respond to stress by regulating transpiration. Transpiration can be correlated with stomatal conductance when the water vapor pressure gradient for transpiration is constant. Thus, our working hypothesis was that the gs measurement could be a useful indicator of the effect of toxic compounds on plants. This lab-scale study aimed to test the measurement of gs as a phytotoxicity indicator...
February 5, 2019: International Journal of Environmental Research and Public Health
Zijian Qin, Yao Xi, Shengde Zhang, Guiping Tu, Aixia Yan
This work reports the classification study conducted on the biggest COX-2 inhibitor dataset so far. Using 2925 diverse COX-2 inhibitors collected from 168 literature, we applied machine learning methods, support vector machine (SVM) and random forest (RF) to develop twelve classification models. The best SVM and RF models resulted in MCC values of 0.73 and 0.72, respectively. The 2925 COX-2 inhibitors were reduced to a dataset of 1630 molecules by removing intermediately active inhibitors and twelve new classification models were constructed, yielding MCC values above 0...
February 14, 2019: Journal of Chemical Information and Modeling
Patrick M McLaurin, Ryan Merritt, Juan C Dominguez, Erico S Teixeira, Jorge A Morales
We present a computational procedure that introduces low degrees of symmetry breaking into a restricted Hartree-Fock (RHF) state in order to induce higher symmetry breaking during the state's subsequent dynamics. The symmetries herein considered are those of electronic HF states as classified by Fukutome; those symmetries affect bond dissociations and internal rotations among other phenomena. Therefore, this investigation extends a part of Fukutome's time-independent analysis of symmetry breaking to the time-dependent (dynamical) regime...
February 14, 2019: Physical Chemistry Chemical Physics: PCCP
Ursula Mirastschijski, Nilima Dinesh, Sudarson Baskaran, Dirk Wedekind, Jelena Gavrilovic, Megan Y Murray, Damon Bevan, Sørge Kelm
Matrix metalloproteinases (MMP) are a family of more than 25 zinc-dependent enzymes that are centrally involved in cellular migration, tissue remodelling, cancer invasion and metastasis. Besides degrading extracellular matrix proteins, MMPs are crucial for growth factor and cytokine release and activation. At the same time, they can inactivate inflammatory mediators and enzymes themselves through protein degradation. Subclasses of MMPs include collagenases, gelatinases, stromelysins, membrane-bound MMPs and others...
February 14, 2019: Wound Repair and Regeneration
Steven D Cothren, Joel N Meyer, Jessica H Hartman
In nearly all subfields of biomedical sciences, there are phenotypes that are currently classified by expert visual scoring. In research applications, these classifications require the experimenter to be blinded to the treatment group in order to avoid unintentional bias in scoring. Currently, many labs either use laborious and tedious methods to manually blind the images, require multiple experimenters to gather and score the data blindly or fail to properly blind the data altogether. In this protocol, we present a simple, freely available software that we created that allows the experimenter to blindly score images...
December 5, 2018: Bio-protocol
Matthew R Jamnik, Lisabeth F DiLalla
Physical and mental health problems are becoming more common among the general population. Studies examining mental and physical health often indicate that mental illness early in life is associated with more detrimental health outcomes later. However, additional work is needed to better identity which psychological problems may contribute to poorer health outcomes. Given recent increases in childhood anxiety and depression specifically, it is beneficial to further investigate the relationship between internalizing problems, both early and later in life, and related health problems...
2019: Frontiers in Psychology
Dennis P O'Malley, Yuhang Yang, Saskia Boisot, Sucha Sudarsanam, Jian-Feng Wang, Vladislav Chizhevsky, Guohua Zhao, Shehla Arain, Lawrence M Weiss
Targeting of the PD1/PD-L1 immune checkpoint pathway has rapidly gained acceptance as a therapeutic strategy for a growing number of malignancies. Testing for expression of PD-L1 in tumor cells and immune cells has been used as a companion or complementary test for drugs targeting the PD1/PD-L1 pathway. We evaluated the results of PD-L1 testing in a large reference lab cohort. Using Food and Drug Administration-approved methods and interpretive instructions for each individual test, 62,896 cases were evaluated for PD-L1 using antibody clone 22C3, 28-8, SP142, or SP263...
February 13, 2019: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
J Gallardo-Gonzalez, A Baraket, S Boudjaoui, T Metzner, F Hauser, T Rößler, S Krause, N Zine, A Streklas, A Alcácer, J Bausells, A Errachid
The present work reports on the development of a new generation of Lab-on-a-chip (LOC) to perform in-situ and real-time potentiometric measurements in flowing water. The device consisted of two differentiated parts: a poly (dimethylsiloxane) (PDMS) microfluidic structure obtained by soft lithography and a fully integrated chemical sensing platform including four working microelectrodes, two reference microelectrodes and one counter microelectrode for detecting ammonium in a continuous mode. The performance of the device was evaluated following its potentiometric response when analyzing ammonium containing samples...
February 25, 2019: Science of the Total Environment
Wasswa William, Andrew Ware, Annabella Habinka Basaza-Ejiri, Johnes Obungoloch
BACKGROUND: Cervical cancer is preventable if effective screening measures are in place. Pap-smear is the commonest technique used for early screening and diagnosis of cervical cancer. However, the manual analysis of the pap-smears is error prone due to human mistake, moreover, the process is tedious and time-consuming. Hence, it is beneficial to develop a computer-assisted diagnosis tool to make the pap-smear test more accurate and reliable. This paper describes the development of a tool for automated diagnosis and classification of cervical cancer from pap-smear images...
February 12, 2019: Biomedical Engineering Online
Pavlína Klusáčková, Jindřiška Lebedová
Occupational asthma and allergic diseases of the upper airway play an important role in occupational diseases. The most frequent allergens, which causes occupational respiratory diseases in the Czech Republic, are isocyanates and wheat and rye flour. Isocyanates are the substances used in car industry during production of polyurethanes, they are in colours and glues. Occupational allergic asthma and rhinitis are diagnosed using specific challenge tests with substances from the workplace. These tests can be done in a special exposure box in a lab with substances taken by hygiene specialists from the patient´s workplace or using direct exposure at the workplace...
2019: Casopís Lékar̆ů C̆eských
Rita Vignani, Pietro Liò, Monica Scali
The varietal authentication of wines is fundamental for assessing wine quality, and it is part of its compositional profiling. The availability of historical, cultural and chemical composition information is extremely important for quality evaluation. DNA-based techniques are a powerful tool for proving the varietal composition of a wine. SSR-amplification of genomic residual Vitis vinifera DNA, namely Wine DNA Fingerprinting (WDF) is able to produce strong, analytical evidence concerning the monovarietal nature of a wine, and for blended wines by generating the probability of the presence/absence of a certain variety, all in association with a dedicated bioinformatics elaboration of genotypes associated with possible varietal candidates...
2019: PloS One
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