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C Li, Y N Wang, M Wang, Y Peng, J T Wu, X F Luo
Objective: To evaluate the feasibility of diffusion parameters obtained from mono-exponential and stretched exponential diffusion-weighted imaging models in staging of nonalcoholic fatty liver disease and compare the diagnosis ability of nonalcoholic steatohepatitis. Methods: Thirty-two 12 -week-old male New Zealand rabbits, about 2.5 kg of each, were randomly divided into five groups. To obtained different severity groups of NAFLD, a variety of diet (from standard to high-fat, high cholesterol chow) were feed for different periods before liver diffusion imaging was performed by using 3...
February 19, 2019: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Zheng-Zheng Zhang, Yun-Feng Zhou, Wei-Ping Li, Chuan Jiang, Zhong Chen, Huan Luo, Bin Song
BACKGROUND: Although many strategies have been developed to modify the biological and biomechanical environment of the meniscal suture repair to improve the chances of healing, the failure rates remain high. Thus, new methods to promote meniscal regeneration and repair are needed. HYPOTHESIS: Administration of magnesium (via a repair using magnesium stitches) might enhance recruitment and adherence of endogenous stem cells to the site of the lesion, thereby promoting in situ meniscal regeneration and chondroprotective functions...
February 20, 2019: American Journal of Sports Medicine
Koshi Akahane, Atsushi Watanabe, Yoshiyuki Furuichi, Shinpei Somazu, Hiroko Oshiro, Kumiko Goi, Kazuo Sakashita, Hideki Muramatsu, Asahito Hama, Yoshiyuki Takahashi, Kenichi Koike, Seiji Kojima, Kanji Sugita, Takeshi Inukai
JMML is an aggressive hematopoietic malignancy of early childhood, and allogeneic HSCT is the only curative treatment for this disease. Umbilical cord blood is one of donor sources for HSCT in JMML patients who do not have an HLA-compatible relative, but engraftment failure remains a major problem. Here, we report two cases of JMML who were successfully rescued by HSCT from an HLA-mismatched parent after development of primary engraftment failure following unrelated CBT. Both patients had severe splenomegaly and underwent unrelated CBT from an HLA-mismatched donor...
February 20, 2019: Pediatric Transplantation
Rodopi Stamatiou, Efrosini Paraskeva, Anna Vasilaki, Apostolia Hatziefthimiou
BACKGROUND: Muscarinic receptor antagonists are a usual treatment for chronic airway diseases, with increased bronchoconstriction, like asthma and chronic obstructive pulmonary disease. These diseases are usually accompanied by airway remodeling, involving airway smooth muscle cell (ASMC) proliferation. The purpose of this study was to examine the effect of the muscarinic receptor modulator gallamine on rabbit tracheal ASMC proliferation. METHODS: ASMCs were incubated with gallamine (1 nM-10 mM), atropine (1 fM-10 mM), and/or acetylcholine (1 nM-1 mM), in the presence or absence of FBS (1% or 10%)...
October 23, 2018: Pharmacological Reports: PR
Blanca Ibarra, Joaquin García-García, Galo Azuara, Blanca Vázquez-Lasa, Miguel A Ortega, Ángel Asúnsolo, Julio San Román, Julia Buján, Natalio García-Honduvilla, Basilio De la Torre
Joint prostheses are an essential element to improve quality of life. However, prostheses may fail due to several factors, including the most frequent cause, Staphylococcus aureus infection. The identification of new fixing bone cements with less reactivity on bone tissue and an adequate response to infection remains a primary challenge. The aim of this study is to evaluate the response of bone tissue in rabbits after introduction of a hydroxyapatite-coated titanium rod with a commercial fixative cement (Palacos®) compared to a modified experimental cement (EC) containing polylactic-co-glycolic acid (PLGA) microspheres in the presence or absence of contaminating germs...
February 19, 2019: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Alicia S Kriete, Natasha Ginzburg, Nima Shah, Richard B Huneke, Emily Reimold, Katsiaryna Prudnikova, Owen Montgomery, J Steve Hou, Evan R Phillips, Michele S Marcolongo
Stress urinary incontinence (SUI), a serious condition which affects ~56% of postmenopausal women, is the involuntary leakage of urine through urethra during physical activity that causes an increase in abdominal pressure. SUI is associated with a decrease in compliance and volume of urethral tissue, likely due to a reduced proteoglycan: collagen ratio in the extracellular matrix and collagen disorganization. Here, we investigated the use of biomimetic proteoglycans (BPGs) to molecularly engineer urethral tissue of New Zealand White rabbits to examine biocompatibility in vivo...
February 19, 2019: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
David C Bloom, Zachary L Watson, Donna M Neumann
Adeno-associated Virus (AAV) vectors are useful vehicles for delivering transgenes to a number of different tissues and organs in vivo. To date, most of these applications deliver the vectors to their target by either infusion into the bloodstream or direct injection into the target tissue. Recently there has been progress in delivering AAV vectors to neurons of the peripheral nervous system (PNS) following application of vectors to the peripheral epithelium, such as the skin or eye. This delivery only requires treatment of the epithelium to access the underlying nerve termini, and following treatment the vectors are transported retrogradely to the cell bodies of these neurons in the ganglia, such as dorsal root ganglia (DRG) or trigeminal ganglia (TG)...
2019: Methods in Molecular Biology
John D Sheppard, Paul C Cockrum, Angela Justice, Mark C Jasek
In the original publication, units were incorrectly published in the results section as micrograms (µg/mL).
February 19, 2019: Ophthalmology and Therapy
Hui Qi, Dan-Ping Liu, Da-Wei Xiao, Da-Chuan Tian, Yong-Wei Su, Shao-Feng Jin
Osteoarthritis (OA) is the most common chronic joint disease worldwide. Chondrocyte, as the only resident cell type in cartilage, its apoptosis is of pathogenetic significance in OA. Mesenchymal stem cell (MSC)-based-therapy has been proved effective in OA in animals and clinical studies. Nowadays, the regenerative potential of MSC-based therapy is mostly attributed to its paracrine secretion, in which exosomes may play an important role. In the present study, we aimed to find out the significance of MSC-derived exosomes (MSC-Exos) on the viability of chondrocytes under normal and inflammatory conditions...
February 19, 2019: In Vitro Cellular & Developmental Biology. Animal
Juanzi Zhang, Wenxian Hu, Qixian Diao, Zhaowei Wang, Jin Miao, Xiao Chen, Zhijun Xue
The present study aimed to explore the therapeutic effect and underlying mechanism of epidermal growth factor (EGF) on the wound healing of diabetic foot ulcers (DFU). A total of 48 rabbits with DFU were randomly divided into 2 groups, comprising the treatment and control groups. Full-thickness skin (10×10 mm) was excised from the thigh of each rabbit. The wounds in the treatment group were treated with 100 mg/l EGF once a day for 1 month. The control group received no treatment. At 20 days following treatment, new granulation tissues that formed beyond the edge of the wound were collected for subsequent analysis...
March 2019: Experimental and Therapeutic Medicine
Franciele Aline Bruinsmann, Stefania Pigana, Tanira Aguirre, Gabriele Dadalt Souto, Gabriela Garrastazu Pereira, Annalisa Bianchera, Laura Tiozzo Fasiolo, Gaia Colombo, Magno Marques, Adriana Raffin Pohlmann, Silvia Stanisçuaski Guterres, Fabio Sonvico
Drug delivery to the brain represents a challenge, especially in the therapy of central nervous system malignancies. Simvastatin (SVT), as with other statins, has shown potential anticancer properties that are difficult to exploit in the central nervous system (CNS). In the present work the physico⁻chemical, mucoadhesive, and permeability-enhancing properties of simvastatin-loaded poly-ε-caprolactone nanocapsules coated with chitosan for nose-to-brain administration were investigated. Lipid-core nanocapsules coated with chitosan (LNCchit ) of different molecular weight (MW) were prepared by a novel one-pot technique, and characterized for particle size, surface charge, particle number density, morphology, drug encapsulation efficiency, interaction between surface nanocapsules with mucin, drug release, and permeability across two nasal mucosa models...
February 18, 2019: Pharmaceutics
Douglas L Feinstein, Kamil Gierzal, Asif Iqbal, Sergey Kalinin, Richard Ripper, Matthew Lindeblad, Alexander Zahkarov, Alexander Lyubimov, Richard van Breemen, Guy Weinberg, Israel Rubinstein
Brodifacoum (BDF) is a potent, long-acting anticoagulant rodenticide that can cause fatal poisoning in humans. The chemical structure of BDF includes 2 chiral carbons, resulting in 2 pairs of diastereomers, BDF-cis (R/S and S/R) and BDF-trans (R/R and S/S). However, the relative potency of these molecules is not known. The purpose of this study was to compare the in vitro and in vivo toxic effects of the 2 BDF diastereomer pairs. In adult Sprague-Dawley rats BDF-cis was significantly more toxic than BDF-trans (LD50 values of 219 versus 316 µg/kg, respectively) while racemic BDF had intermediate potency (266 µg/kg)...
February 16, 2019: Toxicology Letters
Jana Basas, Marta Palau, Xavier Gomis, Benito Almirante, Joan Gavaldà
OBJECTIVE: The aims of this study were as follows. First, we sought to compare the in vitro susceptibility of liposomal amphotericin B (LAmB) and anidulafungin on Candida albicans and Candida glabrata biofilms growing on silicone discs. Second, we sought to compare the activity of LAmB versus anidulafungin for the treatment of experimental catheter-related C. albicans and C. glabrata infections with the antifungal lock technique in a rabbit model. METHODS: Two C...
2019: PloS One
Hiromi Furukawa, Ken Ishikawa, Shigekuni Tsuchiya, Saeko Nishimi, Makoto Sasaki, Chinatsu Onodera, Akira Takada, Kotaro Oyama, Shoichi Chida
BACKGROUND: Overnight catheter drainage (OCD) is introduced to avoid overdistension of the bladder nighttime when clean intermittent catheterization proves ineffective for daytime management of neurogenic bladder. We adopted OCD using disposable silicone no-balloon (DSnB) catheters, with the distal end outside the body opening into diapers. However, the OCD using DSnB catheter had risks of retrograde bacterial contamination. Therefore, in this study, the validity of equipping DSnB catheters with check valves to prevent retrograde bacterial contamination was examined...
February 19, 2019: Pediatrics International: Official Journal of the Japan Pediatric Society
Ronika S Leang, Lisa J Kloft, Brad Gray, Arlene E Gwon, Ling C Huang
INTRODUCTION: To establish the preclinical safety and equivalency of ophthalmic viscosurgical devices (OVDs) comprised of bacterially sourced sodium hyaluronate (HA) to animal sourced HA using pyrogenicity and aqueous exchange models in rabbits and a novel mini-pig model to evaluate corneal endothelial cell protection in vivo. METHODS: HEALON OVD and HEALON5 OVD containing animal-derived HA and HEALON PRO OVD and HEALON5 PRO OVD containing bacterial-derived HA were used...
February 18, 2019: Ophthalmology and Therapy
Gang Liu, Qingyue Li, Chong Wang, Caoling Xu
Coccidiosis is recognized as one of the most widespread and pathogenic parasitic infections in migratory waterfowl throughout the world. It can be caused by several species of Eimeria. We sequenced the complete mitochondrial genome (mtDNA) of Eimeria anseris from wintering greater white-fronted geese (Anser albifrons) in China. The complete E. anseris mtDNA is 6179 bp in size and contains three protein-coding genes (CYT B, COI, and COIII), 12 gene fragments for large subunit ribosomal RNA (rRNA), and seven gene fragments for small subunit rRNA, but no transfer RNA genes...
February 18, 2019: Parasitology Research
Sirorat Thongrod, Chaitip Wanichanon, Prasert Sobhon
Neuropeptide F in invertebrates is a homolog of neuropeptide Y in mammals and it is a member of FMRFamide-related peptides. In arthropods, such as insects, there are two types of neuropeptide F comprising long neuropeptide F (NPF) and short neuropeptide F (sNPF). Both NPFs are known to play a crucial role in the regulations of foraging, feeding-related behaviors, circadian rhythm, stress responses, aggression and reproduction in invertebrates. We have earlier found that in the giant freshwater prawn, Macrobrachium rosenbergii, there are three isoforms of NPF and four isoforms of sNPF and that NPFs are expressed in the eyestalks and brain...
February 19, 2019: Cell and Tissue Research
Jie Xie, Xiang Fang, Xinhua Dai, Bing Shao, Jiancheng Li, You Jiang, Kai Yao, Sihan Wang, Xi Xia, Haiyang Jiang
Pyrenylbutyric acid and streptavidin were coupled to films of reduced graphene oxide (rGO) and then conjugated to a biotinylated broad-spectrum monoclonal antibody against aflatoxins (AFs). It is shown that such films can efficiently and selectively capture AFs inculding AFB1 , AFB2 , AFG1 , AFG2 , AFM1 and AFM2 . The rGO films were characterized by using scanning electron microscopy, energy-dispersive spectroscopy, and raman spectroscopy. The selectivity and purification performance of the antibody-loaded rGO films were investigated...
February 18, 2019: Mikrochimica Acta
Shigeaki Matsuda, Ryu Okada, Sarunporn Tandhavanant, Hirotaka Hiyoshi, Kazuyoshi Gotoh, Tetsuya Iida, Toshio Kodama
Many Gram-negative pathogens utilize dedicated secretion systems to export virulence factors such as exotoxins and effectors1-4 . Several exotoxins are synthesized as precursors containing amino-terminal Sec signal peptides and are exported through the inner-membrane-bound Sec machinery to the periplasm, followed by secretion across the outer membrane to the exterior using a type II secretion system (T2SS)3,5 . Here, we report that thermostable direct haemolysin (TDH), an exotoxin of the food-borne pathogen Vibrio parahaemolyticus, can be exported through the type III secretion system (T3SS), which engages in one-step secretion of effectors4 , despite possessing a Sec signal peptide and being mainly secreted via the T2SS...
February 18, 2019: Nature Microbiology
L Wang, M M Ren, Y L Jian, B X Meng, F L Ma, W J Wang, S Y Guo
Objective: To investigate the effects of the repair and restitution of ear-shaped cartilage by adipose tissue-derived stem cells(ADSC) and cartilage acellular extracellular matrix. Methods: ADSC were extracted by digesting with collagenase type II from the adipose tissue from 32 patients with adiposity whose fats were drawn, and were cultured and subcultured in vitro. The natural biological scaffolds were prepared by acellular method using porcine ear cartilage, and then the second generation ADSC(5.0×10(7)/ml) were inoculated on the preformed natural bio-scaffold scaffold by culturing in vitro for 3 days to form a cell scaffold complex...
February 7, 2019: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
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