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https://read.qxmd.com/read/30677788/targeting-the-hepcidin-ferroportin-pathway-in-anemia-of-chronic-kidney-disease
#1
Matthew Sheetz, Philip Barrington, Sophie Callies, Paul H Berg, Juliet McColm, Thomas Marbury, Brian Decker, Gregory L Dyas, Stephanie M E Truhlar, Robert Benschop, Donmienne Leung, Jolene Berg, Derrick R Witcher
AIMS: Erythropoiesis stimulating agents (ESAs) used to treat anemia in patients with chronic kidney disease (CKD) has been associated with cardiovascular adverse events. Hepcidin production, controlled by BMP6, regulates iron homeostasis via interactions with the iron transporter, ferroportin. High hepcidin levels are thought to contribute to increased iron sequestration and subsequent anemia in CKD patients. To investigate alternative therapies to ESAs for CKD patients, monoclonal antibodies, LY2928057 and LY3113593, targeting ferroportin and BMP6 respectively, were tested in CKD patients...
January 24, 2019: British Journal of Clinical Pharmacology
https://read.qxmd.com/read/30663901/new-4-methoxy-naphthalene-derivatives-as-promisor-antifungal-agents-for-paracoccidioidomycosis-treatment
#2
Mariane C Bagatin, Andrew M F Rozada, Franciele A V Rodrigues, Paulo S A Bueno, Jessyka L Santos, Fernanda Canduri, Érika S Kioshima, Flavio A V Seixas, Ernani A Basso, Gisele F Gauze
AIM: Novel 4-methoxy-naphthalene derivatives were synthesized based on hits structures in order to evaluate the antifungal activity against Paracoccidioides spp. METHODS: Antifungal activity of compounds was evaluated against P. brasiliensis and most promising compounds 2 and 3 were tested against eight clinically important fungal species. RESULTS: Compound 3 was the more active compound with MIC 8 to 32 μg.ml-1 for Paracoccidioides spp without toxicity monkey kidney and murine macrophagecells...
January 21, 2019: Future Microbiology
https://read.qxmd.com/read/30662568/direct-in-vivo-application-of-induced-pluripotent-stem-cells-is-feasible-and-can-be-safe
#3
Meng Xiang, Meng Lu, Jing Quan, Ming Xu, Dan Meng, Anfeng Cui, Ning Li, Yingying Liu, Peng Lu, Xueling Kang, Xiaokai Wang, Ning Sun, Meng Zhao, Qiujuan Liang, Lili Le, Xinhong Wang, Jianyi Zhang, Sifeng Chen
Increasing evidence suggests the consensus that direct in vivo application of induced pluripotent stem cells (iPSCs) is infeasible may not be true. Methods: Teratoma formation and fate were examined in 53 normal and disease conditions involving brain, lung, liver, kidney, islet, skin, hind limb, and arteries. Results: Using classic teratoma generation assays, which require iPSCs to be congregated and confined, all mouse, human, and individualized autologous monkey iPSCs tested formed teratoma, while iPSC-derived cells did not...
2019: Theranostics
https://read.qxmd.com/read/30641362/fluorescein-derived-schiff-base-as-fluorimetric-zinc-ii-sensor-via-turn-on-response-and-its-application-in-live-cell-imaging
#4
Bhriguram Das, Atanu Jana, Ananya Das Mahapatra, Debprasad Chattopadhyay, Anamika Dhara, Subhabrata Mabhai, Satyajit Dey
A novel Schiff base L composed of fluorescein hydrazine and a phenol functionalized moiety has been designed and prepared via cost-effective condensation reaction. The L is utilized for selective sensing of Zn2+ over other environmental and biological relevant metal ions in aqueous alcoholic solution under physiological pH range. The binding of Zn2+ to the receptor L is found to causes ~23 fold fluorescence enhancement of L. The 1:1 binding mode of the metal complex is established by combined UV-Vis, fluorescence, and HRMS (high-resolution mass spectroscopy) spectroscopic methods...
January 2, 2019: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://read.qxmd.com/read/30621774/effect-of-flubendazole-on-developing-stages-of-loa-loa-in-vitro-and-in-vivo-a-new-approach-for-screening-filaricidal-agents
#5
Fanny Fri Fombad, Abdel Jelil Njouendou, Patrick Chounna Ndongmo, Manuel Ritter, Valerine C Chunda, Haelly M Metuge, Narcisse Victor T Gandjui, Peter Enyong, Flobert Njiokou, Achim Hoerauf, Charles D Mackenzie, Samuel Wanji
BACKGROUND: Loiasis, an often-neglected tropical disease, is a threat to the success of lymphatic filariasis and onchocerciasis elimination programmes in rainforest areas of the central and western Africa. Its control and even its elimination might be possible through the use of a safe macrofilaricide, a prophylactic drug, or perhaps a vaccine. This present study evaluated the effect of flubendazole (FLBZ) on the development of Loa loa L3 in vitro and in vivo. METHODS: Infective stages of L...
January 8, 2019: Parasites & Vectors
https://read.qxmd.com/read/30612200/proteomic-analysis-of-monkey-kidney-llc-mk2-cells-infected-with-a-thai-strain-zika-virus
#6
Thamonwan Diteepeng, Sarawut Khongwichit, Atchara Paemanee, Sittiruk Roytrakul, Duncan R Smith
Zika virus (ZIKV) has been endemic in Southeast Asian countries for several years, but the presence of the virus has not been associated with significant outbreaks of infection unlike other countries around the world where the Asian lineage ZIKV was introduced recently. However, few studies have been undertaken using the endemic virus. The Thai isolate was shown to have a similar tissue tropism to an African isolate of ZIKV, albeit that the Thai isolate infected cells at a lower level as compared to the African isolate...
January 5, 2019: Archives of Virology
https://read.qxmd.com/read/30611774/the-efficacy-of-poly-iclc-against-ebola-zaire-virus-ezv-infection-in-mice-and-cynomolgus-monkeys
#7
Meir Kende, Jason Paragas, Andres M Salazar
The potential protection of poly-ICLC (Hiltonol® ) a double stranded RNA (dsRNA) against EZV infection was assessed with prophylactic and therapeutic administration to wild type and TLR3-negative mice, and in non-human primates (NHPs) by measuring EZV serum titers, survival extension, and serum liver and kidney function markers. Various doses of aqueous and liposomal poly-ICLC monotherapy provided robust protection in otherwise lethal murine Ebola virus challenge models, when treatment is started on the day of -or one day after virus challenge...
January 3, 2019: Antiviral Research
https://read.qxmd.com/read/30599414/bio-guided-search-of-active-indole-alkaloids-from-tabernaemontana-catharinensis-antitumour-activity-toxicity-in-silico-and-molecular-modelling-studies
#8
REVIEW
Pauline Fagundes Rosales, Flavio Ferreira Marinho, Adriana Gower, Marilda Chiarello, Bianca Canci, Mariana Roesch-Ely, Favero Reisdorfer Paula, Sidnei Moura
Active plant metabolites have been used as prototype drugs. In this context, Tabernaemontana catharinensis (Apocynaceae) has been highlighted because of the presence of active indole alkaloids. Thus, this study aims the bio-guided search of T. catharinensis cytotoxic alkaloids. The chemical composition was identified by high-resolution mass spectrometry, and fractionation was performed by open column and preparative thin-layer chromatography, from plant stems. The enriched fractions were tested in vitro in tumour cells A375 (melanoma cell line) and A549 (adenocarcinomic human alveolar basal epithelial cells), and non-tumour Vero cells (African green monkey kidney epithelial cells)...
December 15, 2018: Bioorganic Chemistry
https://read.qxmd.com/read/30548114/anti-tumor-effects-of-antibodies-to-l-type-amino-acid-transporter-1-lat1-bound-to-human-and-monkey-lat1-with-dual-avidity-modes
#9
Shiho Ueda, Hidemi Hayashi, Takako Miyamoto, Shinya Abe, Kana Hirai, Kanji Matsukura, Hideki Yagi, Yuta Hara, Kinji Yoshida, Shogo Okazaki, Masakazu Tamura, Yuki Abe, Toshinori Agatsuma, Shin-Ichiro Niwa, Kazue Masuko, Takashi Masuko
L-type amino-acid transporter 1 (LAT1) disulfide-linked to the CD98 heavy chain (hc) is highly expressed in most cancer cells, but weakly expressed in normal cells. In this study, we developed novel anti-LAT1 mAbs, and demonstrated the internalization activity, inhibitory effects of amino acid uptake and cell growth and antibody-dependent cellular cytotoxicity, as well as in vivo anti-tumor effects in athymic mice. Furthermore, we examined the reactivity of mAbs with LAT1 of Macaca fascicularis to evaluate possible side effects of anti-human LAT1 mAbs in clinical trials...
December 10, 2018: Cancer Science
https://read.qxmd.com/read/30547053/quantification-of-infectious-sendai-virus-using-plaque-assay
#10
Narihito Tatsumoto, Takamasa Miyauchi, Moshe Arditi, Michifumi Yamashita
Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae . SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy. Virus quantification is essential not only to determine the original viral titers for an appropriate application, but also to measure the viral titers in samples from the harvests from experiments. There are mainly a couple of units/titers for Sendai viral quantification: plaque-forming units (PFU) and hemagglutination (HA) titer...
November 5, 2018: Bio-protocol
https://read.qxmd.com/read/30536793/quality-bioactivity-study-and-preclinical-acute-toxicity-safety-pharmacology-evaluation-of-pegylated-recombinant-human-endostatin-m-2-es
#11
Lifang Guo, Xingchao Geng, Li Liu, Yufa Miao, Zhi Lin, Min Yu, Yan Fu, Lihong Liu, Bo Li, Yongzhang Luo
Endostar, a potent endogenous antiangiogenic factor, is wildly used in clinics. However, it was easily degraded by enzymes and rapidly cleared by the kidneys. To overcome these shortcomings, PEGylated recombinant human endostatin was developed. In this study, the purity of M2 ES was evaluated by silver stain and reversed-phase high-performance liquid chromatography. Ultraviolet spectrum was used to examine the structural of M2 ES and endostar. The bioactivity and antitumor efficacy of M2 ES were evaluated using an in vitro endothelial cell migration model and athymic nude mouse xenograft model of a heterogeneous lung adenocarcinoma, respectively...
December 10, 2018: Journal of Biochemical and Molecular Toxicology
https://read.qxmd.com/read/30522906/comparison-of-vaccination-with-rhesus-cmv-rhcmv-soluble-gb-with-a-rhcmv-replication-defective-virus-deleted-for-mhc-class-i-immune-evasion-genes-in-a-rhcmv-challenge-model
#12
Sarah Valencia, Rachel B Gill, Kennichi C Dowdell, Yanmei Wang, Ron Hornung, J Jason Bowman, Juan C Lacayo, Jeffrey I Cohen
A human cytomegalovirus (HCMV) vaccine to prevent infection and/or reduce disease associated with congenital infection or visceral disease in transplant recipients is a high priority, but has remained elusive. We created a disabled infectious single cycle rhesus CMV (RhCMV) deleted for glycoprotein L (gL) and the MHC class I immune evasion genes Rh178 and Rh182-189, and restored its epithelial cell tropism by inserting the Rh128-131A genes. The resulting virus, RhCMVRΔgL/178/182-189, was used to vaccinate rhesus monkeys intramuscularly and was compared with vaccination of animals with soluble RhCMV glycoprotein B (gB) in alum/monophosphoryl lipid A or with PBS as a control...
January 7, 2019: Vaccine
https://read.qxmd.com/read/30522424/-evaluation-of-the-reproductive-potential-of-encephalitozoon-intestinalis-in-four-different-cell-line
#13
Ülfet Çetinkaya, Arzuv Charyyeva, Esra Gürbüz
Microsporidia are parasites that can cause infections in many vertebrate and invertebrate organisms and produce small spores resistant to environmental conditions. As they are obligate intracellular parasites, axenic cultures cannot be performed. The aim of this study was to investigate the reproductive potential of the parasite in human colon epidermal adenocarcinoma (Caco-2), human monocytic (U937), African green monkey renal epithelial (VERO) and human kidney epithelial (HEK-293) cell lines of tissue and organs where the parasite is located by following the culture of the parasites and the amount of spores for six weeks...
October 2018: Mikrobiyoloji Bülteni
https://read.qxmd.com/read/30515410/cytotoxic-and-genotoxic-effects-of-fluconazole-on-african-green-monkey-kidney-vero-cell-line
#14
Regianne Maciel Dos Santos Correa, Tatiane Cristina Mota, Adriana Costa Guimarães, Laís Teixeira Bonfim, Rommel Rodriguez Burbano, Marcelo de Oliveira Bahia
Fluconazole is a broad-spectrum triazole antifungal that is well-established as the first-line treatment for Candida albicans infections. Despite its extensive use, reports on its genotoxic/mutagenic effects are controversial; therefore, further studies are needed to better clarify such effects. African green monkey kidney (Vero) cells were exposed in vitro to different concentrations of fluconazole and were then evaluated for different parameters, such as cytotoxicity (MTT/cell death by fluorescent dyes), genotoxicity/mutagenicity (comet assay/micronucleus test), and induction of oxidative stress (DCFH-DA assay)...
2018: BioMed Research International
https://read.qxmd.com/read/30511051/qualitative-and-quantitative-evaluation-of-cytotoxicity-of-five-different-one-step-self-etching-adhesives
#15
Zehra Süsgün Yıldırım, Şeyhmus Bakır, Elif Bakır, Egemen Foto
PURPOSE: To qualitatively and quantitatively compare the cytotoxic potentials of five different one-step self-etching adhesives: Prime&Bond One-Select (PB-OS), Optibond All-in-One (OB-AIO), G-Bond (GB), Clearfil Universal Bond (CUB), Single Bond Universal (SBU). MATERIALS AND METHODS: During the first stage of the study, the cytotoxic activities of the test materials were evaluated qualitatively using the direct contact method. In this method, the test materials were placed directly into a monkey kidney epithelial cell culture medium...
December 3, 2018: Oral Health & Preventive Dentistry
https://read.qxmd.com/read/30508603/in-vitro-analysis-of-synthetic-peptides-in-blocking-the-entry-of-dengue-virus
#16
Asnet Mary John, Akanitt Jittmittraphap, Siriporn Chattanadee, A Alwin Prem Anand, R Shenbagarathai, Pornsawan Leaungwutiwong
Dengue fever is the most prevalent arthropod-borne viral disease, and no specific therapeutic or promising antiviral drug is available for its treatment. Peptide inhibitors are less toxic than synthetic compounds and have found proven effective against viral infections. Here, three peptides that mimic part of the E protein of the dengue virus (DENV) were synthesized and evaluated for their inhibitory activity against four serotypes of DENV in African green monkey kidney (Vero) and rhesus macaque (Macaca mulatta) monkey kidney (LLC-MK2) cell lines...
November 30, 2018: Virus Research
https://read.qxmd.com/read/30488855/-in-vitro-anticancer-anti-inflammatory-and-antioxidant-potentials-of-ephedra-aphylla
#17
Wajdy Al-Awaida, Baker Jawabrah Al-Hourani, Muhanad Akash, Wamidh H Talib, Sima Zein, Rabah Rashad Falah, Zaid Aburubaiha
Purpose: The goal of our study is to test whether a naturally occurring plant, Ephedra aphylla, will show antiproliferative ability against tested cell lines and to test its anti-inflammatory and antioxidative potentials. Materials and Methods: In our study, we used four solvents with different polarities - aqueous, chloroform, methanol, and n-hexane - to extract different compounds from the aerial parts of E. aphylla. Antioxidant activity of E. aphylla was determined by measuring nitric oxide (NO) and hydrogen peroxide (H2 O2 ) scavenging activities...
October 2018: Journal of Cancer Research and Therapeutics
https://read.qxmd.com/read/30477525/aspidosperma-pyrifolium-a-medicinal-plant-from-the-brazilian-caatinga-displays-a-high-antiplasmodial-activity-and-low-cytotoxicity
#18
Isabela P Ceravolo, Carlos L Zani, Flávio J B Figueiredo, Markus Kohlhoff, Antônio E G Santana, Antoniana U Krettli
BACKGROUND: Several species of Aspidosperma plants are referred to as remedies for the treatment of malaria, especially Aspidosperma nitidum. Aspidosperma pyrifolium, also a medicinal plant, is used as a natural anti-inflammatory. Its fractionated extracts were assayed in vitro for activity against malaria parasites and for cytotoxicity. METHODS: Aspidosperma pyrifolium activity was evaluated against Plasmodium falciparum using extracts in vitro. Toxicity towards human hepatoma cells, monkey kidney cells or human monocytes freshly isolated from peripheral blood was also assessed...
November 26, 2018: Malaria Journal
https://read.qxmd.com/read/30428879/croton-gratissimus-leaf-extracts-inhibit-cancer-cell-growth-by-inducing-caspase-3-7-activation-with-additional-anti-inflammatory-and-antioxidant-activities
#19
Emmanuel Mfotie Njoya, Jacobus N Eloff, Lyndy J McGaw
BACKGROUND: Croton species (Euphorbiaceae) are distributed in different parts of the world, and are used in traditional medicine to treat various ailments including cancer, inflammation, parasitic infections and oxidative stress related diseases. The present study aimed to evaluate the antioxidant, anti-inflammatory and cytotoxic properties of different extracts from three Croton species. METHODS: Acetone, ethanol and water leaf extracts from C. gratissimus, C. pseudopulchellus, and C...
November 14, 2018: BMC Complementary and Alternative Medicine
https://read.qxmd.com/read/30423731/designing-heparan-sulfate-based-biocompatible-polymers-and-their-application-for-intracellular-stimuli-sensitive-drug-delivery
#20
Lipeng Qiu, Lu Ge, Miaomiao Long, Qian Wang, Yan Zhang, Xiaotian Shan, Jingxiao Chen, Huijie Zhang, Xiaomei Li, Guozhong Lv, Jinghua Chen
Heparan sulfate (HS) is a kind of natural polysaccharides with good biocompatibility. And as drug carriers, it has some advantages compared to heparin. However, the preparation of HS is cumbersome and difficult, which limits its application in drug delivery. Here, we use modern separation technique combined with chromatography to establish a new preparation method of HS. The molecular weight and degree of dispersion of HS were (1.03 × 104  ± 107) kDa and 1.106, respectively. HS also showed low anticoagulation activity in comparison with heparin...
January 1, 2019: Materials Science & Engineering. C, Materials for Biological Applications
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