Read by QxMD icon Read

serpin in kidney

Sho-Ichi Yamagishi, Takanori Matsui
Pigment epithelium-derived factor (PEDF) is a glycoprotein that belongs to the superfamily of serine protease inhibitors, serpins. It was first identified as a neuronal differentiating factor secreted by human retinal pigment epithelial cells, and then found to be the most potent inhibitor of pathological angiogenesis in mammalian eyes. Recently, PEDF has been shown not only to suppress oxidative stress and inflammatory reactions in vascular wall cells, T cells and macrophages, and adipocytes, but also to exert antithrombotic and anti-fibrotic properties, thereby protecting against the development and progression of various cardiometabolic diseases and related complications...
2018: Current Medicinal Chemistry
Sheon Mary, Heather Yvonne Small, Justyna Siwy, William Mullen, Ashok Giri, Christian Delles
The kidney is centrally involved in blood pressure regulation and undergoes extensive changes during pregnancy. Hypertension during pregnancy may result in an altered urinary peptidome that could be used to indicate new targets of therapeutic or diagnostic interest. The stroke-prone spontaneously hypertensive rat (SHRSP) is a model of maternal chronic hypertension. Capillary electrophoresis-mass spectrometry was conducted to interrogate the urinary peptidome in SHRSP and the control Wistar-Kyoto strain at three time points: prepregnancy and gestational days 12 and 18...
May 2017: Hypertension
Mingmei Ding, Meng Chen, Xiaofang Zhong, Yuhong Wang, Shengli Fu, Xiaoxue Yin, Zheng Guo, Jianmin Ye
C1 inhibitor (C1INH) is a multi-functional serine protease inhibitor in plasmatic cascades, not only inactivating various proteases, but also regulating both complement and contact system activation. In this study, we described the identification and characterization of a C1INH ortholog from Nile tilapia (Oreochromis niloticus) at molecular, protein and cellular levels. The full-length cDNA of Oreochromis niloticus C1INH (OnC1INH) consisted of 1791 bp of nucleotide sequence encoding polypeptides of 596 amino acids...
February 2017: Fish & Shellfish Immunology
Natsuki Kushida, Seitaro Nomura, Imari Mimura, Takanori Fujita, Shogo Yamamoto, Masaomi Nangaku, Hiroyuki Aburatani
BACKGROUND: Kidney injury, including chronic kidney disease and acute kidney injury, is a worldwide health problem. Hypoxia and transforming growth factor-β (TGF-β) are well-known factors that promote kidney injury. Hypoxia-inducible factor (HIF) and SMAD3 are their main downstream transcriptional factors. Hypoxia-HIF pathway and TGF-β/SMAD3 pathway play a crucial role in the progression of kidney injury. However, reports on their interactions are limited, and the global transcriptional regulation under their control is almost unknown...
2016: American Journal of Nephrology
S Samji, H Y Small, S Coley, H Mischak, W Mullen, C Delles
OBJECTIVE: Stroke-prone spontaneously hypertensive rats (SHRSP) are a model of chronic hypertension relative to normotensive Wistar-Kyoto rats (WKY). Our studies on urinary protein and electrolyte levels have shown kidney dysfunction and impaired water balance in pregnant SHRSP. We investigated whether urinary peptidomics can identify target molecules involved in kidney dysfunction in pregnant SHRSP. DESIGN AND METHOD: We analyzed urine of non-pregnant and pregnant WKY and SHRSP (n = 7) at 0th, 12th and 18th gestational day (GD) using capillary electrophoresis on-line coupled to ESI-TOF-MS...
September 2016: Journal of Hypertension
Nicole Rainville, Edward Jachimowicz, Don M Wojchowski
INTRODUCTION: Recombinant human erythropoietin (rhEPO) is a first-line therapeutic for the anemia of chronic kidney disease, cancer chemotherapy, AIDS (Zidovudine therapy), and lower-risk myelodysplastic syndrome. However, rhEPO frequently elevates hypertension, is costly, and may affect cancer progression. Potentially high merit therefore exists for defining new targets for anti-anemia agents within erythropoietin (EPO) and EPO receptor (EPOR) regulatory circuits. AREAS COVERED: EPO production by renal interstitial fibroblasts is subject to modulation by several regulators of hypoxia-inducible factor 2a (HIF2a) including Iron Response Protein-1, prolyl hydroxylases, and HIF2a acetylases, each of which holds potential as anti-anemia drug targets...
2016: Expert Opinion on Therapeutic Targets
Gustavo Núñez-Acuña, Ana Teresa Gonçalves, Valentina Valenzuela-Muñoz, Jorge Pino-Marambio, Simon Wadsworth, Cristian Gallardo-Escárate
One of the most significant threats to the Chilean salmon aquaculture industry is the ectoparasitic sea louse Caligus rogercresseyi. To cope with sea lice infestations, functional diets have become an important component in strengthening the host immune response. The aim of this study was to evaluate molecular mechanisms activated through immunostimulation by in-feed plant-derived additives in Atlantic salmon infected with sea lice. Herein, a transcriptome-wide sequencing analysis was performed from skin and head kidney tissues, evidencing that the immune response genes were the most variable after the challenge, especially in the head kidney, while other genes involved in metabolism were highly expressed individuals fed with the immunostimulants...
November 2015: Fish & Shellfish Immunology
Alaa S Awad, Hanning You, Ting Gao, Anzor Gvritishvili, Timothy K Cooper, Joyce Tombran-Tink
Our recent publication showed that a small bioactive pigment epithelium derived factor (PEDF) peptide (P78-PEDF) prevents the development of diabetic nephropathy (DN). However, its effects on the progression of established DN were not clear. Therefore, the purpose of this study was to determine the effect of P78-PEDF in the progression of DN and to compare the effects of P78-PEDF and an ACE inhibitor (ACEi), a standard of care in DN. Experiments were conducted in Ins2(Akita) mice treated with P78-PEDF or captopril starting at 6 wks of age for 12 wks (early treatment) or starting at 12 wks of age for 6 wks (late treatment)...
2015: PloS One
Milan Obradovic, Nikola Bogdanovic, Djordje Radak, Esma R Isenovic
No abstract text is available yet for this article.
2015: Current Vascular Pharmacology
Jibin Zhang, Jinsoo Ahn, Yeunsu Suh, Seongsoo Hwang, Michael E Davis, Kichoon Lee
Secretory factors in animals play an important role in communication between different cells, tissues and organs. Especially, the secretory factors with specific expression in one tissue may reflect important functions and unique status of that tissue in an organism. In this study, we identified potential tissue-specific secretory factors in the fat, muscle, heart, lung, kidney and liver in the mouse by analyzing microarray data from NCBI's Gene Expression Omnibus (GEO) public repository and searching and predicting their subcellular location in GeneCards and WoLF PSORT, and then confirmed tissue-specific expression of the genes using semi-quantitative PCR reactions...
2015: PloS One
Pengfei Li, Youming Guo, Grant Bledsoe, Zhi-Rong Yang, Hongkuan Fan, Lee Chao, Julie Chao
INTRODUCTION: Kallistatin levels in the circulation are reduced in patients with sepsis and liver disease. Transgenic mice expressing kallistatin are resistant to lipopolysaccharide (LPS)-induced mortality. Here, we investigated the effect of kallistatin on survival and organ damage in mouse models of established sepsis. METHODS: Mice were rendered septic by cecal ligation and puncture (CLP), or endotoxemic by LPS injection. Recombinant human kallistatin was administered intravenously six hours after CLP, or intraperitoneally four hours after LPS challenge...
May 1, 2015: Critical Care: the Official Journal of the Critical Care Forum
Y Bocheva, P Bochev, B Chaushev, S Ivanov
Squamous cell carcinoma antigen (SCC) is isolated as a heterologic antiserum against cells of cervical carcinoma in 1977 by Kato u Torigoe. It is not specific for cervical carcinoma and rises up in cases of carcinoma vulvae, esophageal carcinoma, carcinoma pulmonum, ext. High levels are detected also in psoriasis, sarcoidosis, liver and kidney diseases. SCC is not a reliable marker in diagnosis and screening. Some trials show a correlation between the preterapeutic levels of SCC and the prognosis, but none of them is randomized...
2015: Akusherstvo i Ginekologii︠a︡
Haiyan Hu, Wei Chen, Jiarong Ding, Meng Jia, Jingjing Yin, Zhiyong Guo
Nephrolithiasis is a common kidney disease and one of the major causes of chronic renal insufficiency. We develop and utilize a glyoxylate induced mouse model of kidney calcium oxalate crystal deposition for studying the pharmacological effects of fasudil, a Rho associated protein kinase (ROCK) specific inhibitor, on the kidney injury and fibrosis caused by calcium oxalate crystallization and deposition. Glyoxylate was administrated intraperitoneally to C57BL/6J mice for five consecutive days to establish a mouse model of kidney calcium oxalate crystal formation and deposition...
April 2015: Experimental and Molecular Pathology
Shuqin Zhou, Yingying Sun, Yugang Zhuang, Wei Zhao, Yuanzhuo Chen, Bojie Jiang, Changfeng Guo, Zhonglin Zhang, Hu Peng, Yanqing Chen
BACKGROUND: Kallistatin (KS) is a serine proteinase. The result of KS on 'Renal Ischemia- Reperfusion Injury' (IRI) has not clearly been researched. In this study, investigative research has been conducted to draw results on the administration of human KS on kidney response conducted within a mouse model of IRI. MATERIALS AND METHODS: BALB/c mice were used and given 30 min ischemia that was injected into the kidney which was followed with 24 h reperfusion. The human KS gene contained within an adenoviral vector was injected intravenously 30 min before reperfusion and 12h after reperfusion...
2015: Current Vascular Pharmacology
Songul Yasar Yildiz, Pinar Kuru, Ebru Toksoy Oner, Mehmet Agirbasli
Plasminogen activator inhibitor-1 (PAI-1) is the main inhibitor of plasminogen activators, such as tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA), and a major regulator of the fibrinolytic system. PAI-1 plays a pivotal role in acute thrombotic events such as deep vein thrombosis (DVT) and myocardial infarction (MI). The biological effects of PAI-1 extend far beyond thrombosis including its critical role in fibrotic disorders, atherosclerosis, renal and pulmonary fibrosis, type-2 diabetes, and cancer...
2014: TheScientificWorldJournal
Annalisa Castagna, Sarath Kiran Channavajjhala, Francesca Pizzolo, Oliviero Olivieri
Human urine represents a good source for proteomic research for clinically related studies as it can be collected and processed easily and can give information about kidney-related mechanisms. Little is known about the urinary proteomic changes resulting from physiological (normal), pathological, or environmental variations, and there are few reports on hormone-related modifications of urine proteome. In our study, we highlighted the variations of urinary proteins associated with menstrual cycle or estro-progestin pill in females...
2015: Advances in Experimental Medicine and Biology
Yi-Reng Lin, Chao-Jen Kuo, Hugo You-Hsien Lin, Chin-Jen Wu, Shih-Shin Liang
We synthesized unmodified Fe₃O₄ nanoparticles (NPs) with particles size from 10 nm to 100 nm. We cultured NRK-52E cell lines (rat, kidney) and treated with Fe₃O₄ NPs to investigate and evaluate the cytotoxicity of NPs for NRK-52E cells. Through global proteomics analysis using dimethyl labeling techniques and liquid phase chromatography coupled with a tandem mass spectrometer (LC-MS/MS), we characterized 435 proteins including the programmed cell death related proteins, ras-related proteins, glutathione related proteins, and the chaperone proteins such as heat shock proteins, serpin H1, protein disulfide-isomerase A4, endoplasmin, and endoplasmic reticulum resident proteins...
2014: TheScientificWorldJournal
Elaine Hui, Chun-Yip Yeung, Paul C H Lee, Yu-Cho Woo, Carol H Y Fong, Wing-Sun Chow, Aimin Xu, Karen S L Lam
CONTEXT: Pigment epithelium-derived factor (PEDF), a circulating glycoprotein with antiangiogenic, antioxidative, and anti-inflammatory properties, protects against diabetic nephropathy (DN) in animal models. OBJECTIVE: We investigated whether circulating PEDF predicted the progression of DN in a 4-year prospective study. DESIGN, SETTING, AND PARTICIPANTS: Baseline plasma PEDF levels were measured in type 2 diabetic subjects recruited from the Hong Kong West Diabetes Registry...
November 2014: Journal of Clinical Endocrinology and Metabolism
Meiyu Yan, Bin Su, Wenhui Peng, Liang Li, Hailing Li, Jianhui Zhuang, Yuyan Lu, Weixia Jian, Yidong Wei, Weiming Li, Shen Qu, Yawei Xu
Vaspin and adiponectin are two adipocytokines with antidiabetic effects. Some studies reported that levels of adiponectin and vaspin were correlated with decreased glomerular filtration rate (FGR) and increased albuminuria. We therefore evaluated the vaspin and adiponectin levels in renal insufficiency (RI) patients with or without T2DM. Serum vaspin, adiponectin levels were measured in 416 subjects with or without T2DM. Analysis was made between groups divided by these subjects presence or absence of RI. We found that serum adiponectin level was significantly higher in nondiabetic patients with RI than in nondiabetic subjects without RI; however, there were no statistical differences between the diabetic patients with RI and without RI...
2014: Journal of Diabetes Research
Xiao-Hua Zhang, Min-Li Li, Bin Wang, Mei-Xia Guo, Ren-Min Zhu
AIM: To assess the effect of inhibition of caspase-1 on acute renal injury in rats with severe acute pancreatitis (SAP). METHODS: Forty-two Sprague-Dawley rats were randomly divided into three groups: healthy controls (HC, n = 6), SAP rats treated with saline (SAP-S, n = 18), or SAP rats treated with a caspase-1/interleukin (IL)-1β-converting-enzyme (ICE) inhibitor (SAP-I-ICE, n = 18). SAP was induced by retrograde infusion of 5% sodium taurocholate into the bile-pancreatic duct...
August 14, 2014: World Journal of Gastroenterology: WJG
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"