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Francis Vallières, Isabelle Durocher, Denis Girard
The biological roles of interleukin (IL)-21 in human monocytes and macrophages have been neglected. We previously demonstrated that IL-21 induce phagocytosis and established that Syk is a new molecular target of IL-21. Herein, we found that IL-21 is not chemoattractant for immature THP-1 and primary monocytes but can increase the capacity of THP-1 cells (not primary monocytes) to adhere onto a cell substratum by a Syk-dependent mechanism without altering the expression of a panel of cell surface molecules. Unlike THP- 1 and monocytes, IL-21 can increase metalloproteinase (MMP)-9 secretion and activity in monocyte-derived macrophages (HMDM), as assessed by western blot and zymography experiments, respectively...
February 8, 2019: Cellular Immunology
Soukaina Motia, Ioan Albert Tudor, Paulo Antonio Ribeiro, Maria Raposo, Benachir Bouchikhi, Nezha El Bari
Triclosan (TCS) is a topical antiseptic widely used in different cosmetic products. It is also a common additive in many antimicrobial household consumables. Over a certain concentration, it becomes risky for human and environmental health. This work describes the development of an electrochemical sensor based on molecularly imprinted polymer (MIP), assembled on screen-printed gold electrode (Au-SPE), dedicated to the TCS detection in environmental water sources. To achieve this goal, an acrylamide/bisacrylamide solution was polymerized after linking TCS with the carboxylic polyvinyl chloride (PVC-COOH) layer onto the Au-SPE...
January 26, 2019: Science of the Total Environment
Zijie Zhang, Juewen Liu
Molecular imprinting refers to templated polymerization with rationally designed monomers, and this is a general method to prepare stable and cost-effective ligands. This attractive concept however suffers from low affinity, low specificity, and limited signaling mechanisms for binding. Acrydite-modified DNA oligonucleotides can be readily copolymerized into acrylic polymers. With molecular recognition and catalytic functions, such functional DNAs are recently shown to enhance the performance of molecularly imprinted polymers (MIPs) in a few ways...
February 13, 2019: Small
Anna Nelson, Clayton L Camic, Carl Foster, Brooke Zajac, Kaela Hoecherl, Jacob Erickson, Andrew R Jagim
Multiple ingredient pre-workout supplements (MIPS) are purported to offer favorable acute metabolic changes potentially leading to improvements in body composition and training adaptations over time. However, there is limited information available regarding the long-term benefits and safety of consuming MIPS, specifically in female populations. Therefore, the purpose of this study was to investigate the effects of pre-workout ingestion on body composition, training adaptations and select markers of clinical health in recreationally active females after 7 weeks of supplementation and resistance training...
2019: International Journal of Exercise Science
Gizem Ert Uuml Rk Bergdahl, Tilde Andersson, Maria Allhorn, Sofie Yngman, Rainer Timm, Rolf Lood
In this study, a surface plasmon resonance biosensor was developed for the detection and quantification of a secreted bacterial factor (RoxP) from skin. Molecular imprinting method was used for the preparation of sensor chips and five different monomer-cross linker compositions were evaluated for sensitivity, selectivity, affinity and kinetic measurements. The most promising MIP was characterized by using scanning electron microscopy, atomic force microscopy, and cyclic voltammetry. Limit of detection (LOD) value was calculated as 0...
February 13, 2019: ACS Sensors
Jingjing Xu, Franck Merlier, Bérangère Avalle, Vincent Vieillard, Patrice Debré, Karsten Haupt, Bernadette Tse Sum Bui
We describe the preparation and characterization of synthetic antibodies based on molecularly imprinted polymer nanoparticles (MIP-NPs), for the recognition and binding of the highly conserved and specific peptide motif SWSNKS (3S), an epitope of the envelope glycoprotein 41 (gp41) of human immunodeficiency virus type 1 (HIV-1). This motif is implicated in the decline of CD4+ T cells and leads to the deterioration of the immune system during HIV infection. Therefore, the development of MIP-NPs that can target and block the 3S peptide to prevent subsequent cascade interactions directed toward the killing of CD4+ T cells is of prime importance...
February 13, 2019: ACS Applied Materials & Interfaces
Sabina Herrera, Wajiha Gohir, Farid Foroutan, Claire Aguilar, Stephen Juvet, Tereza Martinu, Deepali Kumar, Atul Humar, Coleman Rotstein, Shaf Keshavjee, Lianne G Singer, Shahid Husain
We studied cytokine patterns of LTRs with Aspergillus spp colonization who did not progress to IPA in the absence of antifungal prophylaxis. Retrospective, single center case-control study. BAL samples were analyzed for cytokines. Patients with Aspergillus spp. in BAL who didn't receive prophylaxis and didn't develop IPA were compared to LTRs with Aspergillus spp that received prophylaxis, LTRs with IPA and controls. 21 patients with Aspergillus colonization who did not develop IPA, 7 patients with suspected IPA who received prophylaxis, 4 IPA and 19 controls were included...
February 12, 2019: Transplant Infectious Disease: An Official Journal of the Transplantation Society
Stacey Piotrowski, Lindsay Wilson, Neeraja Dharmaraj, Amani Hamze, Ashley Clark, Ramesh Tailor, Lori Hill, Stephen Lai, F Kurtis Kasper, Simon Young
INTRODUCTION: Tissue engineering technologies aiming to enhance maxillofacial wound healing are often tested in vivo in pre-clinical models that do not necessarily reflect the complexity of the clinical need. The aim of this study was to develop a rabbit model of compromised craniofacial wound healing that more accurately mimics clinical scenarios. MATERIALS AND METHODS: An experimental group of rabbits (n= 10) received fractionated radiation of the mandible totaling 36 Gy...
February 12, 2019: Tissue Engineering. Part C, Methods
Botond Szucs, Mate Petrekanits, Janos Varga
Background: Chronic obstructive pulmonary disease (COPD) may have considerable cardiovascular risk. Physical activity has a paramount role in COPD treatment. Our aim was to evaluate the applicability of arteriograph in COPD and measure the effectiveness of pulmonary rehabilitation on endothelial function. Methods: A total of 40 patients with COPD (FEV1 : 45.43±20.20%pred, BMI: 27.99±6.98 kg/m2 , male: female was 21:19, age: 65.47±7.39 years) participated in a 4-week rehabilitation program...
December 2018: Journal of Thoracic Disease
Erich Müller, Patrick Pröller, Fatima Ferreira-Briza, Lorenz Aglas, Thomas Stöggl
Purpose: We set out to investigate the effectiveness of grounded sleeping on the time course of recovery with respect to muscle soreness and athletic performance after intensive eccentric muscle loading. Methods: Twenty-two healthy participants were recruited for this study and randomly assigned to an experimental group (GRD, grounded sleeping, n = 12) or control group (UGD, sham-grounded sleeping, n = 10) to evaluate the effects of 10 days recovery with GRD vs. UGD following a single intensive downhill treadmill intervention in a triple-blinded (participant, tester, and data analyst) manner...
2019: Frontiers in Physiology
Katrijn Gijbels, Sheldon Landsberger, Pieter Samyn, Remus Ion Iacobescu, Yiannis Pontikes, Sonja Schreurs, Wouter Schroeyers
Alkali-activated materials (AAMs) based on ground granulated blast furnace slag (GGBFS) and phosphogypsum (PG) were investigated in order to quantify leaching of naturally occurring radionuclides (NOR) and inorganic non-radiological elements according to an up-flow percolation column test as described in CEN/TS 16637-3. Gamma spectroscopy and neutron activation analysis (NAA) were applied for radiological characterization, inductively coupled plasma optical emission spectrometry (ICP-OES) and ion-chromatography (IC) for chemical characterization...
April 10, 2019: Science of the Total Environment
J Garcia Lopez, E V Piletska, M J Whitcombe, J Czulak, S A Piletsky
A novel bacterial quorum quenching system is presented. For the first time the degradation of N-l-hexanoyl homoserine lactone (C6-AHL), a Gram-negative quorum sensing autoinducer, has been enhanced using molecularly imprinted nanoparticles (MIP NPs) which were prepared using transition state analogue of the γ-lactone ring hydrolysis as template.
February 11, 2019: Chemical Communications: Chem Comm
Yu Zhang, Rui Zhang, Xiaolin Yang, Honglan Qi, Chengxiao Zhang
Electrogenerated chemiluminescence (electrochemiluminescence, ECL) generates species at electrode surfaces, which undergoes electron-transfer reactions and forms excited states to emit light. It has become a very powerful analytical technique and has been widely used in such as clinical testing, biowarfare agent detection, and pharmaceutical analysis. This review focuses on the current trends of molecular recognition-based biosensing methods for pharmaceutical analysis since 2010. It introduces a background of ECL and presents the recent ECL developments in ECL immunoassay (ECLIA), immunosensors, enzyme-based biosensors, aptamer-based biosensors, and molecularly imprinted polymers (MIP)-based sensors...
February 2019: Journal of Pharmaceutical Analysis
YuLan Liang, QianYu Zhao, HongDong Liu, XiaoTian Chen, DongMing Zhang, XueWen Lai, Bin Li, Xin Yang
A general method for efficient and selective extraction of a target compound from complex natural products remains elusive, despite decades of research. By introducing a functional amido group on the surface of dispersity-enhanced magnetic nanoparticles, a nanoparticle receptor to selectively recognize Sibiskoside (a monoterpene) from the aerial portion of Sibiraea angustata by hydrogen bond interaction was synthesized. The superparamagnetic Fe3 O4 nanoparticles were successively modified with tetraethyl orthosilicate (TEOS), amino and amido functional groups, and 4-vinylbenzoic acid (VBZA) was used as the functional monomer...
January 18, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Bernie M Bissett, Jiali Wang, Teresa Neeman, I Anne Leditschke, Robert Boots, Jennifer Paratz
BACKGROUND: Inspiratory muscle training (IMT) increases inspiratory muscle strength and improves quality of life in intensive care unit (ICU) patients who have been invasively mechanically ventilated for ≥7 days. The purpose of this study was to identify which patients benefit most from IMT following weaning from mechanical ventilation. METHODS: Secondary analysis of a randomized trial of supervised daily IMT in 70 patients (mean age 59 years) in a 31-bed ICU was carried out...
February 9, 2019: Physiotherapy Theory and Practice
Maryam Bakhshizadeh, Seyed Ahmad Mohajeri, Habibollah Esmaily, Seyed Amir Aledavood, Fatemeh Varshoei Tabrizi, Mahmoud Seifi, Farzin Hadizadeh, Ameneh Sazgarnia
BACKGROUND: Some materials such as TiO2 display a luminescence property when exposed to X-ray radiation. Therefore, a proper photosensitizer can induce photodynamic effects by absorbing the emitted photons from these materials during radiotherapy. In this way, the problem of limited photo- penetration depth in photodynamic therapy is resolved. In this paper, following the production of a nanopolymer containing TiO2 cores and imprinted for mitoxantron (MIP), the possibility of utilizing its optical and radio properties on two lines of cancer cells were studied...
February 6, 2019: Photodiagnosis and Photodynamic Therapy
Heng Yao, Yunjia Shi, Jihong Guan, Shuigeng Zhou
Identifying protein complexes is helpful for understanding cellular functions and designing drugs. In the last decades, many computational methods have been proposed based on detecting dense subgraphs or subnetworks in Protein-Protein Interaction Networks (PINs). However, the high rate of false positive/negative interactions in PINs prevents from the achievement of satisfactory detection results directly from PINs, because most of such existing methods exploit mainly topological information to do network partitioning...
February 6, 2019: IEEE/ACM Transactions on Computational Biology and Bioinformatics
Ewa Grudzińska, Sławomir Grzegorczyn, Zenon P Czuba
The role of cytokines in the pathogenesis of chronic venous disease (CVD) remains obscure. It has been postulated that oscillatory flow present in incompetent veins causes proinflammatory changes. Our earlier study confirmed this hypothesis. This study is aimed at assessing chemokines and growth factors (GFs) released by lymphocytes in patients with great saphenous vein (GSV) incompetence. In 34 patients exhibiting reflux in GSV, blood was derived from the cubital vein and from the incompetent saphenofemoral junction...
2019: Mediators of Inflammation
Kiyofumi Yamada, Shinichi Yoshimura, Manabu Shirakawa, Kazutaka Uchida, Shigehiro Nakahara, Shuji Nishida, Yasuhiro Iwamoto, Yoshikazu Sato, Masanori Kawasaki
Carotid intraplaque hemorrhage (IPH) plays a critical role in the progression of carotid atherosclerotic disease. IPH was associated with high intensity signal (HIS) in the plaque on Maximum intensity projection (MIP) images from routine three dimensional magnetic resonance imaging (3D-TOF MRA). The aim of this study was to evaluate the relationships among HIS, new ipsilateral ischemic stroke and a progression rate in carotid plaques with moderate stenosis. We included 45 carotid plaques with moderate stenosis (50%-69%) in 45 patients who could be followed more than 12 months...
February 4, 2019: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
Chung-Yin Lin, Sheng-Hung Tsai, Dar-Fu Tai
The cystine-bridged cyclic peptide hormones (CBCPHs) represent signature structural feature as well as unique biological activity. In this study, three CBCPHs have been identified and characterized, namely, oxytocin, atrial natriuretic peptides (ANPs), and brain natriuretic peptides (BNPs). Because research has shown that ANPs and BNPs are powerful diagnostic biomarkers for heart disease, a highly laudable endeavor would be to develop a novel sensor for detecting ANP or BNP levels. Therefore, an amphiphilic monomer Acr-His-NHNH-Fmoc was synthesized to form molecularly imprinted polymers (MIPs) for targeted CBCPH detection...
February 5, 2019: Journal of Peptide Science: An Official Publication of the European Peptide Society
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