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https://read.qxmd.com/read/30747617/expression-of-human-ficolin-2-in-hepatocytes-confers-resistance-to-infection-by-diverse-hepatotropic-viruses
#1
Paywast J Jalal, Richard A Urbanowicz, Emma Horncastle, Monika Pathak, Chun Goddard, Amanj Saeed, Christopher P Mason, Jonathan K Ball, William L Irving, C Patrick McClure, Barnabas J King, Alexander W Tarr
The liver-expressed pattern recognition receptors mannose-binding lectin (MBL), ficolin-2 and ficolin-3 contribute to the innate immune response by activating complement. Binding of soluble ficolin-2 to viral pathogens can directly neutralize virus entry. We observed that the human hepatoma cell line HuH7.5, which is routinely used for the study of hepatotropic viruses, is deficient in expression of MBL, ficolin-2 and ficolin-3. We generated a cell line that expressed and secreted ficolin-2. This cell line (HuH7...
February 12, 2019: Journal of Medical Microbiology
https://read.qxmd.com/read/30747207/identification-of-critical-genes-associated-with-spinal-cord-injury-based-on-the-gene-expression-profile-of-spinal-cord-tissues-from-trkb-t1-knockout-mice
#2
Li Wei, Fei He, Wen Zhang, Wenhua Chen, Bo Yu
The present study aimed to identify the genes and underlying mechanisms critical to the pathology of spinal cord injury (SCI). Gene expression profiles of spinal cord tissues of trkB.T1 knockout (KO) mice following SCI were accessible from the Gene Expression Omnibus database. Compared with trkB.T1 wild type (WT) mice, the differentially expressed genes (DEGs) in trkB.T1 KO mice following injury at different time points were screened out. The significant DEGs were subjected to function, co‑expression and protein‑protein interaction (PPI) network analyses...
January 22, 2019: Molecular Medicine Reports
https://read.qxmd.com/read/30743201/association-between-the-ratio-of-aryl-hydrocarbon-receptor-ahr-in-th17-cells-to-ahr-in-treg-cells-and-sle-skin-lesions
#3
Haitao Yu, Lili Jiang, Ruiqi Liu, Aimin Yang, Xuemei Yang, Liming Wang, Wei Zhang, Tuanjie Che
Skin lesions are typical clinical manifestations of systemic lupus erythematosus (SLE) and the biomarker for predicting SLE skin injury is not clear. We conducted a hospital-based case-control study with aim to explore the predictive value of the ratio of aryl hydrocarbon receptor (AhR) in T helper 17 (Th17) cells to AhR in regulatory T (Treg) cells (AhR ratio) in SLE skin lesions. The clinical and laboratory data were obtained from their medical records, and the AhR relative expression levels were evaluated by reverse transcription-quantitative polymerase chain reaction...
February 8, 2019: International Immunopharmacology
https://read.qxmd.com/read/30735814/few-plasmodium-falciparum-merozoite-ligand-and-erythrocyte-receptor-pairs-show-evidence-of-balancing-selection
#4
Lynette Isabella Ochola-Oyier, Kevin Wamae, Irene Omedo, Christabel Ogola, Abneel Matharu, Jean Pierre Musayibimana, Francis K Njogu, Kevin Marsh
Erythrocyte surface proteins have been identified as receptors of Plasmodium falciparum merozoite proteins. The ligand-receptor interactions enable the parasite to invade human erythrocytes, initiating the clinical symptoms of malaria. These interactions are likely to have had an evolutionary impact on the genes that encode the ligand and receptor proteins. We used sequence data from Kilifi, Kenya to detect departures from neutrality in a paired analysis of P. falciparum merozoite ligands and their erythrocyte receptor genes from the same population...
February 5, 2019: Infection, Genetics and Evolution
https://read.qxmd.com/read/30734226/immuno-pharmacological-characterization-of-presynaptic-glun3a-containing-nmda-autoreceptors-relevance-to-anti-nmda-receptor-autoimmune-diseases
#5
Guendalina Olivero, Matteo Vergassola, Francesca Cisani, Cesare Usai, Anna Pittaluga
Mouse hippocampal glutamatergic nerve endings express presynaptic release-regulating NMDA autoreceptors (NMDARs). The presence of GluN1, GluN2A, GluN2B, and GluN3A subunits in hippocampal vesicular glutamate transporter type 1-positive synaptosomes was confirmed with confocal microscopy. GluN2C, GluN2D, and GluN3B immunopositivity was scarcely present. Incubation of synaptosomes with the anti-GluN1, the anti-GluN2A, the anti-GluN2B, or the anti-GluN3A antibody prevented the 30 μM NMDA/1 μM glycine-evoked [3 H]D-aspartate ([3 H]D-ASP) release...
February 7, 2019: Molecular Neurobiology
https://read.qxmd.com/read/30713336/autophagy-and-oncosis-necroptosis-are-enhanced-in-cardiomyocytes-from-heart-failure-patients
#6
Giovanni Corsetti, Carol Chen-Scarabelli, Claudia Romano, Evasio Pasini, Francesco S Dioguardi, Francesco Onorati, Richard Knight, Hemang Patel, Louis Saravolatz, Giuseppe Faggian, Tiziano M Scarabelli
BACKGROUND Although originally described as a survival mechanism, it is unknown whether and to what extent autophagy is implicated in the terminal stages of heart failure. Here, we studied magnitude and evolution of autophagy in patients with intractable heart failure. MATERIAL AND METHODS Myocardial samples were obtained from 22 patients with ischemic cardiomyopathy and idiopathic dilated cardiomyopathy who were undergoing cardiac transplantation. Hearts from 11 patients who died from non-cardiac causes were used as control samples...
February 4, 2019: Medical Science Monitor Basic Research
https://read.qxmd.com/read/30712871/natural-killer-cells-degenerate-intact-sensory-afferents-following-nerve-injury
#7
Alexander J Davies, Hyoung Woo Kim, Rafael Gonzalez-Cano, Jahyang Choi, Seung Keun Back, Seung Eon Roh, Errin Johnson, Melanie Gabriac, Mi-Sun Kim, Jaehee Lee, Jeong Eun Lee, Yun Sook Kim, Yong Chul Bae, Sang Jeong Kim, Kyung-Mi Lee, Heung Sik Na, Priscilla Riva, Alban Latremoliere, Simon Rinaldi, Sophie Ugolini, Michael Costigan, Seog Bae Oh
Sensory axons degenerate following separation from their cell body, but partial injury to peripheral nerves may leave the integrity of damaged axons preserved. We show that an endogenous ligand for the natural killer (NK) cell receptor NKG2D, Retinoic Acid Early 1 (RAE1), is re-expressed in adult dorsal root ganglion neurons following peripheral nerve injury, triggering selective degeneration of injured axons. Infiltration of cytotoxic NK cells into the sciatic nerve by extravasation occurs within 3 days following crush injury...
January 25, 2019: Cell
https://read.qxmd.com/read/30710623/crispr-cas9-mediated-knockout-of-both-the-pxabcc2-and-pxabcc3-genes-confers-high-level-resistance-to-bacillus-thuringiensis-cry1ac-toxin-in-the-diamondback-moth-plutella-xylostella-l
#8
Zhaojiang Guo, Dan Sun, Shi Kang, Junlei Zhou, Lijun Gong, Jianying Qin, Le Guo, Liuhong Zhu, Yang Bai, Liang Luo, Youjun Zhang
Rapid evolution of resistance by insect pests severely jeopardizes the sustainable utilization of biopesticides and transgenic crops that produce insecticidal crystal proteins derived from the entomopathogenic bacterium Bacillus thuringiensis (Bt). Recently, high levels of resistance to Bt Cry1 toxins have been reported to be genetically linked to the mutation or down-regulation of ABC transporter subfamily C genes ABCC2 and ABCC3 in seven lepidopteran insects, including Plutella xylostella (L.). To further determine the causal relationship between alterations in the PxABCC2 and PxABCC3 genes and Cry1Ac resistance in P...
January 30, 2019: Insect Biochemistry and Molecular Biology
https://read.qxmd.com/read/30696582/activated-glycine-receptors-may-decrease-endosomal-nadph-oxidase-activity-by-opposing-clc-3-mediated-efflux-of-chloride-from-endosomes
#9
Mark F McCarty, Simon Iloki-Assanga, Lidianys Maria Lewis Lujan, James J DiNicolantonio
Receptor-mediated activation of NADPH oxidase complexes commonly occurs in endosomes; the hydrogen peroxide produced by the dismutation of superoxide generated within the endosomes often functions to boost receptor function by reversibly inhibiting protein tyrosine phosphatases or by promoting formation of signaling complexes. NADPH oxidase-mediated formation of superoxide entails transfer of two electrons (provided by NADPH) from the cytosol to the endosomal lumen, where two molecules of superoxide are generated...
February 2019: Medical Hypotheses
https://read.qxmd.com/read/30695890/-orai1-variation-induced-combined-immunodeficiency-a-case-report-and-literature-review
#10
H Y Yang, X L Deng, F Yin, J Peng, L W Wu
Objective: To summarize the clinical manifestations and gene variations of combined immunodeficiency caused by ORAI1 variation with a case report and literature review. Methods: The clinical data of the patient who was diagnosed with ORAI1 variation caused combined immunodeficiency in the Department of Pediatrics in Xiangya Hospital of Central South University in February 2018 were extracted and analyzed. The literature till August 2018 was searched with key words of 'ORAI1', and 'immunodeficiency' in both English and Chinese in the database of China national knowledge infrast ructure (CNKI), Wanfang and Pubmed...
February 2, 2019: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://read.qxmd.com/read/30689631/differential-immunoglobulin-and-complement-levels-in-leprosy-prior-to-development-of-reversal-reaction-and-erythema-nodosum-leprosum
#11
Francianne M Amorim, Maurício L Nobre, Larissa S Nascimento, Alesson M Miranda, Glória R G Monteiro, Francisco P Freire-Neto, Maria do Carmo Palmeira Queiroz, José W Queiroz, Malcolm S Duthie, Marcos R Costa, Steven G Reed, Warren D Johnson, Kathryn M Dupnik, Selma M B Jeronimo
BACKGROUND: Leprosy is a treatable infectious disease caused by Mycobacterium leprae. However, there is additional morbidity from leprosy-associated pathologic immune reactions, reversal reaction (RR) and erythema nodosum leprosum (ENL), which occur in 1 in 3 people with leprosy, even with effective treatment of M. leprae. There is currently no predictive marker in use to indicate which people with leprosy will develop these debilitating immune reactions. Our peripheral blood mononuclear cell (PBMC) transcriptome analysis revealed that activation of the classical complement pathway is common to both RR and ENL...
January 28, 2019: PLoS Neglected Tropical Diseases
https://read.qxmd.com/read/30684458/exploring-electrostatic-interactions-of-relaxin-family-peptide-receptor-3-and-4-with-ligands-using-a-nanobit-based-binding-assay
#12
Jia-Hui Wang, Wei-Han Nie, Xiao-Xia Shao, Hao-Zheng Li, Meng-Jun Hu, Ya-Li Liu, Zeng-Guang Xu, Zhan-Yun Guo
Relaxin family peptides perform a variety of biological functions by activating four G protein-coupled receptors, namely relaxin family peptide receptor 1-4 (RXFP1-4). We recently disclosed electrostatic interactions of the homologous RXFP3 and RXFP4 with some agonists based on activation complementation. However, this activation assay-based approach cannot be applied to antagonists that do not activate receptors. Herein, we propose a general approach suitable for both agonists and antagonists based on our newly-developed NanoBiT-based binding assay...
January 23, 2019: Biochimica et biophysica acta. Biomembranes
https://read.qxmd.com/read/30678306/integrative-analyses-of-genes-associated-with-subcutaneous-insulin-resistance
#13
Manoj Kumar Pujar, Basavaraj Vastrad, Chanabasayya Vastrad
: Insulin resistance is present in the majority of patients with non-insulin-dependent diabetes mellitus (NIDDM) and obesity. In this study, we aimed to investigate the key genes and potential molecular mechanism in insulin resistance. Expression profiles of the genes were extracted from the Gene Expression Omnibus (GEO) database. Pathway and Gene Ontology (GO) enrichment analyses were conducted at Enrichr. The protein⁻protein interaction (PPI) network was settled and analyzed using the Search Tool for the Retrieval of Interacting Genes (STRING) database constructed by Cytoscape software...
January 22, 2019: Biomolecules
https://read.qxmd.com/read/30670763/vaccine-induced-antibodies-target-sequestered-viral-antigens-to-prevent-ocular-hsv-1-pathogenesis-preserve-vision-and-preempt-productive-neuronal-infection
#14
Derek J Royer, Joshua F Hendrix, Chelsea M Larabee, Alaina M Reagan, Virginie H Sjoelund, Danielle M Robertson, Daniel J J Carr
The cornea is essential for vision yet highly sensitive to immune-mediated damage following infection. Generating vaccines that provide sterile immunity against ocular surface pathogens without evoking vision loss is therefore clinically challenging. Here, we tested a prophylactic live-attenuated vaccine against herpes simplex virus type 1 (HSV-1), a widespread human pathogen that can cause corneal blindness. Parenteral vaccination of mice resulted in sterile immunity to subsequent HSV-1 challenge in the cornea and suppressed productive infection of the nervous system...
January 22, 2019: Mucosal Immunology
https://read.qxmd.com/read/30656202/data-on-inflammatory-cytokines-and-pathways-involved-in-clearance-of-nontypeable-haemophilus-influenzae-from-the-lungs-during-cigarette-smoking-and-vitamin-d-deficiency
#15
Jef Serré, Carolien Mathyssen, Tom Tanjeko Ajime, Hannelie Korf, Karen Maes, Nele Heulens, Conny Gysemans, Chantal Mathieu, Bart Vanaudenaerde, Wim Janssens, Ghislaine Gayan-Ramirez
This article contains data related to the inflammatory cytokine and investigated pathways involved in bacterial clearance reported in "Airway infection with Nontypeable Haemophilus influenzae is more rapidly eradicated in vitamin D deficient mice" (Serré et al., 2018) [1]. Vitamin D deficient or sufficient mice were oropharyngeally instilled with 106 NTHi and sacrificed at 4, 8, 24 and 72 h post-infection. We measured proinflammatory cytokines (KC, TNF-α, IL-1β, IL6 and MCP-1) markers of bacterial clearance pathways (myeloid peroxidase, nitric oxide, complement C5a and immunoglobulin A) in bronchoalveolar fluid (BALF) during infection and mRNA expression levels of innate immune defense mechanism markers (mucin glycoproteins, pathogen recognitions receptor TLR2 and TLR4, antimicrobial peptides SLPI, REG3γ, lysozyme, BD-1, BD-2, BD-3 and surfactant proteins SP-A and SP-D) in lung homogenate...
February 2019: Data in Brief
https://read.qxmd.com/read/30651113/memantine-potentiates-cytarabine-induced-cell-death-of-acute-leukemia-correlating-with-inhibition-of-k-v-1-3-potassium-channels-akt-and-erk1-2-signaling
#16
Theresa Lowinus, Florian H Heidel, Tanima Bose, Subbaiah Chary Nimmagadda, Tina Schnöder, Clemens Cammann, Ingo Schmitz, Ulrike Seifert, Thomas Fischer, Burkhart Schraven, Ursula Bommhardt
BACKGROUND: Treatment of acute leukemia is challenging and long-lasting remissions are difficult to induce. Innovative therapy approaches aim to complement standard chemotherapy to improve drug efficacy and decrease toxicity. Promising new therapeutic targets in cancer therapy include voltage-gated Kv 1.3 potassium channels, but their role in acute leukemia is unclear. We reported that Kv 1.3 channels of lymphocytes are blocked by memantine, which is known as an antagonist of neuronal N-methyl-D-aspartate type glutamate receptors and clinically applied in therapy of advanced Alzheimer disease...
January 16, 2019: Cell Communication and Signaling: CCS
https://read.qxmd.com/read/30647151/complement-targets-newborn-retinal-ganglion-cells-for-phagocytic-elimination-by-microglia
#17
Sarah R Anderson, Jianmin Zhang, Michael R Steele, Cesar O Romero, Amanda G Kautzman, Dorothy P Schafer, Monica L Vetter
Microglia play important roles in shaping the developing central nervous system, and at early stages they have been proposed to regulate progenitor proliferation, differentiation, and neuronal survival. However, these studies reveal contradictory outcomes, highlighting the complexity of these cell-cell interactions. Here, we investigate microglia function during embryonic mouse retina development, where only microglia, progenitors, and neurons are present. In both sexes, we determine that microglia primarily interact with retinal neurons and find that depletion of microglia via conditional knockout of the Csf1 receptor results in increased density of retinal ganglion cells (RGCs)...
January 15, 2019: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://read.qxmd.com/read/30616520/interaction-between-tp53-and-xrcc1-increases-susceptibility-to-cervical-cancer-development-a-case-control-study
#18
Gui-Cen Liu, Yun-Fei Zhou, Xiao-Chao Su, Jun Zhang
BACKGROUND: Cervical cancer is the 4th highest cause of female reproductive tract malignancies. Multiple loci have been identified as important determinant factors for tumor susceptibility. In this report, we aimed to explore the roles of gene polymorphisms affecting x-ray repair cross complementing 1 (XRCC1), the tumor protein p53 (TP53), and fibroblast growth factor receptor 3 (FGFR3) in the context of susceptibility to cervical cancer. Additionally, we assessed the impact of single nucleotide polymorphism-single nucleotide polymorphism (SNP-SNP) interaction of these three genes in the context of cervical cancer risk in Chinese women...
January 7, 2019: BMC Cancer
https://read.qxmd.com/read/30614533/characterization-of-the-epithelial-membrane-protein-3-interaction-network-reveals-a-potential-functional-link-to-mitogenic-signal-transduction-regulation
#19
Arne Christians, Eric Poisel, Christian Hartmann, Andreas von Deimling, Stefan Pusch
Epithelial Membrane Protein 3 (EMP3), a 4-transmembrane glycoprotein, first gained attention as a putative tumor suppressor. Accumulating evidence, however, points to a more tumor promotive function of EMP3. The biological function of EMP3 is still largely unclear. To elucidate more of EMP3's interaction network, we performed a Yeast-Two-Hybrid (Y2H) screening, followed by validation of candidate interactors by Biomolecular Fluorescence Complementation (BiFC) and Proximity Ligation Assay (PLA). Furthermore, we generated stable EMP3 knockdown cell lines and measured cell proliferation, migration and sensitivity to apoptosis induction as well as the expression and activation levels of important signal pathway components...
January 7, 2019: International Journal of Cancer. Journal International du Cancer
https://read.qxmd.com/read/30599993/curdlan-oligosaccharides-having-higher-immunostimulatory-activity-than-curdlan-in-mice-treated-with-cyclophosphamide
#20
Jiqing Tang, Hongmin Zhen, Nannan Wang, Qiaojuan Yan, Hao Jing, Zhengqiang Jiang
This study evaluated the immunostimulatory activity of curdlan oligosaccharides (GOS) in cyclophosphamide (CTX)-induced immunosuppressed mice and in RAW264.7 cells. GOS was able to stimulate the release of nitric oxide (NO), cytokines (IL-1β, IL-6 and TNF-α) and improve the phagocytic rate of peritoneal macrophages and RAW264.7 cells. It further enhanced immunoglobulins (Ig) release (IgG by 50.6%-74.7%, IgA by 31.3%-34.9%, IgM by 28.3%-66.7%), splenic lymphocyte proliferation (by 74.8%-91.3%), nature killer cells cytotoxicity (by 32...
March 1, 2019: Carbohydrate Polymers
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