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Congshan Liu, Jiaqing Yao, Jianhai Yin, Jian Xue, Haobing Zhang
Echinococcosis, which causes a high disease burden and is of great public health significance, is caused by the larval stage of Echinococcus species. It has been suggested that tubulin is the target of benzimidazoles, the only drugs for the treatment of echinococcosis. This study evaluated the characteristics of tubulins from Echinococcus granulosus. The full-length cDNAs of E. granulosus α- and β-tubulin isoforms were cloned by reverse transcription PCR from protoscolex RNA. Then, these two tubulin isoforms (α9 and β4 ) were recombinantly expressed as insoluble inclusion bodies in Escherichia coli...
2018: Parasite: Journal de la Société Française de Parasitologie
Congshan Yang, Jing Liu, Lei Ma, Xichen Zhang, Xiao Zhang, Bingxin Zhou, Xingquan Zhu, Qun Liu
Neospora caninum is an obligate intracellular protozoan parasite that infects a wide range of mammalian species, and particularly causes the reproductive loss in cattle. We identified a novel dense granule protein, N. caninum granule protein 17 (NcGRA17) using the CRISPR/cas9 genome editing system and studied its function. We generated the NcGRA17 knockout strain (ΔNcGRA17) and NcGRA17 complementary strain (iΔNcGRA17). Plaque assays and intracellular proliferation tests showed that the ΔNcGRA17 strain formed smaller plaques and had slower intracellular growth...
December 15, 2018: Veterinary Parasitology
Yong Fu, Xia Cui, Jing Liu, Xiao Zhang, Heng Zhang, Congshan Yang, Qun Liu
Toxoplasma gondii relies on apicoplast-localized FASII pathway and endoplasmic reticulum-associated fatty acid elongation pathway for the synthesis of fatty acids, which flow through lipid metabolism mainly in the form of long-chain acyl-CoA (LCACoAs) esters. Functions of Toxoplasma acyl-CoA transporters in lipid metabolism remain unclear. Here, we investigated the roles of acyl-CoA-binding protein (TgACBP1) and a sterol carrier protein-2 (TgSCP2) as cytosolic acyl-CoA transporters in lipid metabolism. The fluormetric binding assay and yeast complementation confirmed the acyl-CoA binding activities of TgACBP1 and TgSCP2, respectively...
October 26, 2018: Cellular Microbiology
Xiaochun Xue, Jianhua Wu, Junhui Li, Jianguo Xu, Haiying Dai, Congshan Tao, Chao Li, Jinhong Hu
It was previously reported that the expression of CD274 was down-regulated in psoriatic epidermis, leading to immune disorders of psoriasis. However, the regulatory mechanisms of CD274 were rarely elucidated. We aimed to explore the regulatory mechanisms of CD274.Skin samples were collected from 18 patients with psoriasis vulgaris and 9 healthy participants for RNA sequencing. Candidate genes were chosen based on degree and k-core difference of genes in the co-expression network. The relations between candidate genes and CD274 were validated by flow cytometry and realtime PCR in primary human epidermal keratinocytes...
October 19, 2018: Bioscience Reports
Jing Huang, Zhiqiang Huang, Xiangjun Zhou, Chao Xia, Muhammad Imran, Shujuan Wang, Congshan Xu, Manrong Zha, Yan Liu, Cankui Zhang
The vasculature of higher plants is important with transport of both nutrient and information molecules. To understand the correspondence of this tissue in molecular responses under phosphate (Pi) deficiency, Plantago major, a model plant for vasculature biology study, was chosen in our analysis. After RNA-Seq and de novo transcriptome assembly of 24 libraries prepared from the vasculature of P. major, 37,309 unigenes with a mean length of 1571 base pairs were obtained. Upon 24 h of Pi deficiency, 237 genes were shown to be differentially expressed in the vasculature of P...
September 28, 2018: Molecular Genetics and Genomics: MGG
Sarah Logan, Congshan Jiang, Yasheng Yan, Yasuyoshi Inagaki, Thiago Arzua, Xiaowen Bai
BACKGROUND: Propofol induces acute neurotoxicity (e.g., neuroapoptosis) followed by impairment of long-term memory and learning in animals. However, underlying mechanisms remain largely unknown. Long non-coding RNAs (lncRNAs) are found to participate in various pathological processes. We hypothesized that lncRNA profile and the associated signaling pathways were altered, and these changes might be related to the neurotoxicity observed in the neonatal mouse hippocampus following propofol exposure...
2018: Cellular Physiology and Biochemistry
Congshan Jiang, Sarah Logan, Yasheng Yan, Yasuyoshi Inagaki, Thiago Arzua, Peizhong Ma, Shemin Lu, Zeljko J Bosnjak, Xiaowen Bai
Mounting evidence has demonstrated that general anesthetics could induce acute neuroapoptosis in developing animals followed by long-term cognitive dysfunction, with the mechanisms remaining largely unknown. The aim of this study was to investigate the effect of the intravenous anesthetic propofol on the profiles of microRNAs (miRNAs) and messenger RNAs (mRNAs), and their interactive signaling networks in the developing mouse hippocampus. Postnatal day 7 (P7) mice were exposed to propofol for 3 hours. Hippocampi were harvested from both P7 (3 hours after exposure) and P60 mice for the analysis of the expression of 726 miRNAs and 24,881 mRNAs, and apoptosis...
September 21, 2018: Scientific Reports
Congshan Wan, Thomas K Gaylord, Muhannad S Bakir
A grating-assisted-cylindrical-resonant-cavities (GARC) interlayer coupler made of Si/SiO2 is designed and simulated to achieve efficient and broadband interlayer coupling. This coupler consists of three cylindrical resonant cavities: two waveguide cavities in the horizontal direction and one cylindrical via cavity in the vertical direction. The resonant strengths of the two cylindrical waveguide cavities are enhanced by circular Bessel-function-defined gratings and distributed Bragg reflectors. The interlayer coupling efficiency of this Si/SiO2 GARC coupler is simulated as ηc =68%(-1...
June 20, 2018: Applied Optics
Congshan Xu, Chao Xia, Zhiqiang Xia, Xiangjun Zhou, Jing Huang, Zhiqiang Huang, Yan Liu, Yiwei Jiang, Shaun Casteel, Cankui Zhang
The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at their vegetative stages. It is known that the reproductive stage of soybean is more susceptible to drought. Therefore, an understanding on the responsive mechanisms during this stage will not only be important for basic plant physiology, but the knowledge can also be used for crop improvement via either genetic engineering or molecular breeding...
December 2018: Plant Cell Reports
Jianhai Yin, Yujuan Shen, Aiping Yu, Congshan Liu, Jiaqing Yao, Wenci Gong, Jianping Cao
The aim of this study was to first evaluate the proangiogenic activity of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) in mice infected with Echinococcus granulosus. PMN-MDSCs derived from experimentally infected mice were collected and cultured in vitro, and their effect on angiogenesis was investigated using a human umbilical vein endothelial cell (HUVEC) tube-formation assay stimulated with the supernatant by microscope and the Angiogenesis module of the software NIH Image J. In addition, the expression levels of several functional factors related to proangiogenic activity were analyzed...
2018: Bioscience Trends
Kai Ma, Congshan Wang, Jie Yang, Changchun Hua, Xinping Guan
In this paper, a pricing mechanism is proposed for the electricity supply chain, which is consisting of one generation company (GC), multiple consumers, and competing utility companies (UCs). The UC participates in electricity supply chain management by a revenue sharing contract (RSC). In the electricity supply chain, the electricity real-time balance has an important role in the stable operation of the power system. Therefore, we introduce the demand response into the electricity supply chain to match supply with demand under forecast errors...
November 9, 2017: IEEE Transactions on Cybernetics
Yuanxu Guo, Zixin Min, Congshan Jiang, Wei Wang, Jidong Yan, Peng Xu, Ke Xu, Jing Xu, Mengyao Sun, Yitong Zhao, Safdar Hussain, Rui Zhang, Quancheng Wang, Yan Han, Fujun Zhang, Wenhua Zhu, Dongmin Li, Liesu Meng, Jian Sun, Shemin Lu
Osteoarthritis (OA) is the most common form of arthritis involving major structural changes of peripheral joints and local or systemic inflammation and in lack of therapeutic approaches because of complexity of underlying molecular basis. Our previous work showed that HS6ST2, an enzyme involved in the transfer of sulfate, is downregulated in cartilage tissues of OA patients compared with normal donors, but little is known about its regulatory mechanism. In this study, we demonstrated that the expression of HS6ST2 was lower in OA-damaged cartilage than smooth cartilage from the same patient...
June 13, 2018: Cell Death & Disease
Zehao Li, Xu Zhou, Lidan Li, Shue Liu, Congshan Wang, Lina Li, Changyuan Yu, Xin Su
Hybridization-based probes emerge as a promising tool for nucleic acid target detection and imaging. However, the single-nucleotide selectivity is still challenging because the specificity of hybridization reaction is typically low at room temperature. We disclose an effective and simple method for highly selective detection and in situ imaging of single-nucleotide mutation (SNM) by taking the advantages of the specific hybridization of short duplex and the signal amplifying effect of cationic conjugated polymer (CCP)...
June 5, 2018: Analytical Chemistry
Nazim Hussain, Wenhua Zhu, Congshan Jiang, Jing Xu, Manman Geng, Xiaoying Wu, Safdar Hussain, Bo Wang, Muhammad Shahid Riaz Rajoka, Yue Li, Juan Tian, Liesu Meng, Shemin Lu
Synoviocytes from rheumatoid arthritis (RA) patients share certain features with tumor cells, such as over proliferation and invasion. Anomalous microRNA (miRNA) expression may participate in the pathogenesis of RA in different ways. The objective of the present study was to observe the role of miR-10a-5p targeting T-box transcription factor 5 (TBX5) gene on synoviocyte proliferation and apoptosis in RA. Human synovial sarcoma cell line, SW982 cells stimulating with interleukin-1β (IL-1β) were transfected with miR-10a-5p mimic and siRNA of TBX5...
April 27, 2018: Bioscience Reports
Jianhai Yin, Congshan Liu, Yujuan Shen, Haobing Zhang, Jianping Cao
BACKGROUND: Cystic echinococcosis is a global public health problem; however, the drugs (albendazole and mebendazole) currently recommended by WHO for its treatment, have limited efficacy. Therefore, novel drugs are required to provide more choices for the treatment of this disease. METHODS: The anthelmintic effects of ursolic acid (UA) were tested on Echinococcus granulosus protoscoleces, germinal cells and metacestodes in vitro. The in vivo efficacy of UA was investigated in mice following secondary infection with E...
January 24, 2018: Parasites & Vectors
Biquan Xiong, Gang Wang, Congshan Zhou, Yu Liu, Panliang Zhang, Kewen Tang
An efficient phosphorylation of C(sp3 )-H bonds of arenes with diaryl phosphinic acids via Bu4 NI-catalyzed dehydrogenative coupling has been developed. This transformation proceeds efficiently under transition-metal-free reaction conditions and represents a straightforward method to prepare valuable organophosphorus compounds from readily available arenes and diaryl phosphinic acids.
January 19, 2018: Journal of Organic Chemistry
Bo Wang, Panpan Zhao, Yan Zhou, Liesu Meng, Wenhua Zhu, Congshan Jiang, Linyu Wang, Yongsong Cai, Shemin Lu, Weikun Hou
The objective of this study was to identify the key changed subtype of T helper cells (Th cells) and their cytokines in pristane-induced arthritis (PIA) in rats. The severity of arthritis was evaluated by body weight, clinical score, the perimeter of ankle and mid-paw and histological assessment of ankle joints. Cytokines of Th1, Th2 and Th17 were determined in the spleen and inguinal lymph nodes at 28 days after pristane injection by real-time qPCR. The mRNA levels of IL-22 receptors, IL-22R1 and IL-22BP, in the spleen were quantified by real-time qPCR...
2017: PloS One
Yasheng Yan, Shigang Qiao, Chika Kikuchi, Ivan Zaja, Sarah Logan, Congshan Jiang, Thiago Arzua, Xiaowen Bai
It has been shown that propofol can induce widespread apoptosis in neonatal mouse brains followed by long-term cognitive dysfunction. However, selective brain area and cell vulnerability to propofol remains unknown. This study was aimed to dissect toxic effect of propofol on multiple brain cells, including neurons, astrocytes, oligodendrocytes, and neural stem cells (NSCs). Seven-day-old mice were intraperitoneally administrated propofol or intralipid as a vehicle control for 6 hours. To identify vulnerable cells undergoing apoptosis following propofol exposure, brain sagittal sections were co-stained with antibodies against an apoptosis marker along with neuron, astrocyte, oligodendrocyte, or NSC markers using immunofluorescence staining...
October 14, 2017: Brain Sciences
Congshan Liu, Jianhai Yin, Jian Xue, Yi Tao, Wei Hu, Haobing Zhang
Cystic echinococcosis is a globally distributed zoonotic disease, which is caused by the larval stage of Echinococcosus granulosus sensu lato. The chemotherapy of the disease is limited to the use of benzimidazoles. Recently, mefloquine and its analogues, aminoalcohol-carbazole, and some amino alcohol derivatives were reported to display inhibitory effects on parasites. Here, the activities of 130 amino alcohol compounds against E. granulosus were tested on protoscoleces and germinal cells at a concentration of 20 μg/ml over a period of three days...
June 2018: Acta Tropica
Nazim Hussain, Wenhua Zhu, Congshan Jiang, Jing Xu, Xiaoying Wu, Manman Geng, Safdar Hussain, Yongsong Cai, Ke Xu, Peng Xu, Yan Han, Jian Sun, Liesu Meng, Shemin Lu
MicroRNAs are considered to play critical roles in the pathogenesis of human inflammatory arthritis, including rheumatoid arthritis (RA). The purpose of this study was to determine the relationship between miR-10a-5p and TBX5 in synoviocytes and evaluate their contribution to joint inflammation. The expression of miR-10a-5p and TBX5 in the synovium of RA and human synovial sarcoma cell line SW982 stimulated by IL-1β was determined by RT-qPCR and Western blotting. The direct interaction between miR-10a-5p and TBX5 3'UTR was determined by dual-luciferase reporter assay in HeLa cells...
January 2018: Journal of Cellular and Molecular Medicine
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