Eugene Y Chan, Nuno M Goncalves, Rebecca A Haeusler, Amie J Hatch, Jonathan W Larson, Anthony M Maletta, Gregory R Yantz, Eugene D Carstea, Martin Fuchs, Gordon G Wong, Steven R Gullans, Rudolf Gilmanshin
We have developed a rapid molecular mapping technology--Direct Linear Analysis (DLA)--on the basis of the analysis of individual DNA molecules bound with sequence-specific fluorescent tags. The apparatus includes a microfluidic device for stretching DNA molecules in elongational flow that is coupled to a multicolor detection system capable of single-fluorophore sensitivity. Double-stranded DNA molecules were tagged at sequence-specific motif sites with fluorescent bisPNA (Peptide Nucleic Acid) tags. The DNA molecules were then stretched in the microfluidic device and driven in a flow stream past confocal fluorescence detectors...
June 2004: Genome Research