Nicole Huge, Thea Reinkens, Reena Buurman, Maria Sandbothe, Anke Bergmann, Hannah Wallaschek, Beate Vajen, Amelie Stalke, Melanie Decker, Marlies Eilers, Vera Schäffer, Oliver Dittrich-Breiholz, Engin Gürlevik, Florian Kühnel, Brigitte Schlegelberger, Thomas Illig, Britta Skawran
BACKGROUND: In hepatocellular carcinoma (HCC), histone deacetylases (HDACs) are frequently overexpressed. This results in chromatin compaction and silencing of tumor-relevant genes and microRNAs. Modulation of microRNA expression is a potential treatment option for HCC. Therefore, we aimed to characterize the epigenetically regulated miR-129-5p regarding its functional effects and target genes to understand its relevance for HCC tumorigenesis. METHODS: Global miRNA expression of HCC cell lines (HLE, HLF, Huh7, HepG2, Hep3B) and normal liver cell lines (THLE-2, THLE-3) was analyzed after HDAC inhibition by miRNA sequencing...
May 16, 2022: Cancer Cell International