Camila Alves-Mondini, Martina Beltramino, Isabella Gizzi Jiacomini, Sabrina Karim-Silva, Nicolle Dos Santos Antunes, Juliana de Moura, Nicolas Aubrey, Philippe Billiald, Ricardo Andrez Machado-de-Ávila, Larissa M Alvarenga, Alessandra Becker-Finco
In the Americas and specially in Brazil, the Loxosceles intermedia, Loxosceles gaucho and Loxosceles laeta are the three most medically relevant brown spider species, and whose bites can lead to the condition known as loxoscelism. Here, we report the development of a tool capable of identifying a common epitope amongst Loxosceles sp. venom's toxins. A murine monoclonal antibody (LmAb12) and its recombinant fragments (scFv12P and diabody12P) have been produced and characterized. This antibody and its recombinant constructs were able to recognize proteins of Loxosceles spider venoms with specificity...
July 1, 2023: International Journal of Biological Macromolecules