Kenneth P Hough, Jennifer L Trevor, Balu K Chacko, John G Strenkowski, Yong Wang, Kayla F Goliwas, Nathaniel B Bone, Young-Il Kim, Renita Holmes, Shia Vang, Alexandra Pritchard, Jay Chin, Sandeep Bodduluri, Veena B Antony, Sultan Tousif, Mohammad S Athar, Diptiman Chanda, Kasturi Mitra, Jaroslaw Zmijewski, Jianhua Zhang, Steven R Duncan, Victor J Thannickal, Susanne Gabrielsson, Victor M Darley-Usmar, Jessy S Deshane
RATIONALE: Asthma is a chronic inflammatory disease of the airways that involves crosstalk between myeloid-derived regulatory cells (MDRCs) and CD4+ T cells. Although small extracellular vesicles (sEVs) are known to mediate cell-cell communication, the role of sEV signaling via mitochondria in perpetuating asthmatic airway inflammation is unknown. OBJECTIVES: We investigated the effects of MDRC-derived exosomes on dysregulated T cell responses in asthmatics. METHODS: Small extracellular vesicles isolated from bronchoalveolar lavage fluid or airway MDRCs of mild to moderate asthmatics or healthy controls were co-cultured with autologous peripheral and airway CD4+ T lymphocytes...
May 3, 2024: bioRxiv